Pharmaceutical Sciences and Research (PSR)
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Uji Aktivitas Anti-Inflamasi Minyak Atsiri Daun Kemangi (Ocimum americanum L.) pada Tikus Putih Jantan yang Diinduksi Karagenan
Get PDFKemangi (Ocimum americanum L.) is an aromatic plant that contains citral and known as anti-inflammatory agents. The aimed of this study was to determine the anti-inflammatory activity of the essential oil kemangi leaves on carrageenan-induced rat. The male rats were divided into six groups, each consisting of four rats. Group I as negative control was given 0.5% CMC solution, group II as positive control was given diclofenac sodium, group III was given sitral, groups IV, V and VI were given 40 mg/200 g BW, 80 mg/200 g BW, and 160 mg/200 g BW essential oil of Ocimum americanum L., emulsified in 0.5% CMC solution. After 30 minutes of administration, left paw of rats was injected bycarragenan 0.2 mL to induce edema. Edema volume was measured using pletismometer every hour for six hours. The result showed that at dose 160 mg/200 g BW gives the best effect in inhibited the inflamation response 44,83%. There was significant difference (p<0.05) at dose 160 mg/200 g BB to negative control. From this study can be concluded that essential oil kemangi leaves has anti-inflammatory activity
FORMULASI DAN UJI KESTABILAN FISIK SUSPENSI TOPIKAL YANG MENGANDUNG EKSTRAK NERII FOLIUM SEBAGAI ANTIBAKTERI DALAM SEDIAAN ANTI JERAWAT
No full textNerium oleander is one of the plants that can be developed to be a herbal medicine because the leaf extract has an antibacterial activity against some microorganisms and empirically had been used to solve the acne problem. The anti acne formulation should not make the acne worse because of mistaken to choose the dosage form. The suspension dosage form (has no oil content makes the acne worse) containing the dried leaf extract of Nerium oleander in 97% alcohol was chosen to be formulated by varying respectively carbomer, sodium carboxy methyl cellulose and tragacanth as a suspending agent. The concentration of Nerii folium extract in the suspension was based on the Minimal Inhibitory Concentration of the extract against Propionibacterium acnes and Staphylococcus. The evaluation of the formula was done by physical stability test including organoleptic test (color and odor), pH, volume of sedimentation, mean particle diameter size, viscosity stored in low temperature of 4oC, room tperature of 28oC, and high temperature of 40oC, cycling test and mechanical test.The results showed that suspension with sodium carboxy methyl cellulose as the suspending agent has better physical stability than carbomer or tragacanth
PENGARUH PEMBERIAN EKSTRAK BIJI KLABET (TRIGONELLA FOENUM-GRAECUM L.) TERHADAP KADAR HORMON ESTRADIOL DAN FSH PLASMA TIKUS PUTIH BETINA GALUR WISTAR PREPUBERTAL
No full textBiji Klabet or Fenugreek (Trigonella foenum-graecum L.) contains steroidal sapogenins such as diosgenin, tigogenin, gitogenin, yamogenin and trigoneoside, that are suspected having an estrogen-like effect or as phytoestrogen. The objective of this study was to evaluate the estrogen-like effect of biji klabet alcohol extracts. This research used immature Wistar female rats, age of 19 days, which were represent the climacteric/menopause phase. The testing animals were divided into five groups, namely normal group, estradiol control group and three level dose groups of fenugreek extract (30 mg/200 gBW; 60 mg/200 gBW and 120 mg/200 gBW). The result showed that on 120 mg/200 gBW fenugreek extract significantly (a=0.05) increased estradiol and FSH level on immature rat. Analysis of vaginal smear showed vaginal lubrication effect, surprisingly on 120 mg/200 gBW could induce estrous cycle
Pemodelan Molekul Turunan p-Metoksi sinnamoil Hidrazida Sebagai Inhibitor Checkpoint Kinase 1 dan Inhibitor Aromatase secara In silico
Get PDFThe development of anticancer drugs from ethyl p-methoxycinnamate (EPMC) derivatives continue to obtain compounds that have high ability of cancer cells apoptosis and minimal side effects. Compounds p-Methoxycinnamoyl hydrazide derivatives from EPMC structure modification were docked into the ligand-binding pocket of Check point kinase 1 enzymes (2YWP) and the aromatase enzyme (3S7S) using software Molegro Virtual Docker (MVD) Ver.5.5.We compared the Rerank score of native ligand with derived compounds p-Methoxycinnamoyl hydrazide. Rerank score of Compound 4b and 4c (-99.98 Kcal/mol and -99.80Kcal/mol) is lower than the native ligand A42 in inhibiting the enzyme checkpoint kinase 1. Rerank value of compounds p-Methoxycinnamoyl hydrazide derivatives is greater than the native ligand EXM in inhibiting the enzyme aromatase.Compounds p-Methoxycinnamoyl hydrazide derivatives especially compound 4b and 4c have anticancer mechanism by inhibiting the enzyme pathway checkpoint kinase 1 and have not activity in inhibiting the enzyme aromatase
Perbandingan Efektifitas Antara Minyak Atsiri Kulit Batang Kayu Manis (Cinnamomum burmannii) dengan Temephos sebagai Larvasida Aedes aegypti
Get PDFDengue fever is a major health issue in the world. Preventive measure with temephos/abate has long been used to combat this problem. Istiana et al reported that there were cases of resistance of Aedes aegypti larvae to temephos in various parts of the world, including Indonesia. Indonesian Cinnamon (Cinnamomum burmannii) has been proven to possess larvacide effect and can be used as an alternative insecticide. This research was meant to compare the larvacide effect of Indonesian cinnamon (Cinnamomum burmannii) essential oil to the larvacide effect of temephos against Aedes aegypti larvae. This research used laboratory experimental design analysis. The total number of samples were 20 larvae of Aedes aegypti at 3rd and 4th instar which were tested four times on each four experimental groups. The collected data were the total number of Aedes aegypti larva deaths observed 24 hours after the variables were applied. The analysis of the results was done by using statistic analysis program. There were significant differences on larva deaths due to the essential oil and abate variables with p value<0,001. The results were as follows: negative control death (0%), emulsifier (2,5%), essential oil (22,5%), and abate (100%). The larvacide effectiveness of abate is significantly better than that of Indonesian cinnamon essential oil
ANALISIS EPIGALOKATEKIN GALAT DALAM MINUMAN TEH HIJAU SIAP SAJI
No full textEpigallocatechin gallat (EGCg) is a polyphenol constituent in tea that has antioxidant effect. The purpose of this research was to identify and examine qualitative and quantitative EGCg in green tea preparations which were stored in different condition, that was in a refrigerator and in an open air. The results showed that all samples containing EGCg. The examination of EGCg concentration in the sample was done by thin layer chromatography densitometric method using cellulose plate. N-propanol - acetic acid - water (1:1:5) was the selected eluent. Linear regression equation from calibration curve was y = -1204.071 + 7.495 x, with correlation factor (r) = 0.99911. EGCg concentration from sample that stored in refrigerator sample number one was 0.00293% w/v, sample number two was 0.00298% w/v, and sample number three was 0.00328% w/v. EGCg concentration from sample that stored in open air was lower than LOD
Efektivitas SNEDDS Ekstrak Kulit Manggis Terhadap Bakteri P. mirabilis dan S. epidermidis yang Terdapat pada Ulkus Diabetik
Get PDFMangosteen rind has the ability to conduct antibacterial activity. Under the development relating to drug delivery system, SNEDDS were known to boost the penetration of active compound. The aim of this research was to compare the effectiveness of the antibacterial activity at both SNEDDS preparation and the ethanol extract of mangosteen rind as the antibacteria against the bacteria that cause diabetic ulcers with the most prevalence whether it was Gram positive or Gram negative bacteria like Proteus mirabilis and Staphylococcus epidermidis. The research started by maceration process with the crude extract as the result continued with the phytochemical screening which subsequently prepared as SNEDDS preparations. The SNEDDS preparation prepared earlier then analyzed to see the content of the active compound using spectrophotometer UV-Vis and the measuring of antibacteria activity with bacteria growth inhibitory parameter at ethanol extract SNEDDS preparations of mangosteen rind then compared to ciprofloxacin and were analyzed the data afterwards using ANOVA. The results shows that SNEDDS preparation of mangosteen extract have an activity against both typed of bacteria that cause diabetic ulcers shows by significant differences between both group which is P. mirabilis bacteria group and S. epidermidis bacteria group in ANOVA test with significant number of 0,000 and shows significant differences between both group
EPIDEMIOLOGI DAN DIAGNOSIS DENGUE DI INDONESIA
No full textDengue fever/DF and dengue hemorrhagic fever/DHF is a global public health problem that occured in tropical and subtropical region. Epidemic dengue occurs every years, and it continues to be a major health problem in Indonesia. Due to its asymptomatic nature, a reliable, rapid and accurate dengue diagnosis is needed. Dengue diagnosis method based on molecular dengue virus properties and it will be developed by researcher. Dengue rapid test isnewly method. This article explaine about dengue epidemiology, molecular dengue virus properties, clinical diagnosis, serology diagnosis and progress research of dengue virus
Aktivitas Antioksidan Ekstrak, Fraksi dan Golongan Senyawa Kimia Daun Premna oblongata Miq.
Get PDFantioxidant activity of Cincau Perdu leaves extract (Premna oblongata Miq.) using 1-Diphenyl-2-picrylhydrazyl (DPPH) methods. P. oblongata leaves was extracted using n-hexane, ethylacetate and methanol. It was found that methanol extract has the highest activity with IC50 value 20.01 μg/mL. The extract with highest antioxidant activity were fractionated with accelerated column chromatography and were earned 6 combination fractions. The antioxidant activity of each combination fractions were tested by DPPH methods. Fraction-5 showed the highest activity as antioxidant with IC50 23.51 μg/mL. The compounds of the active fractions were flavonoid, glikon, saponin and tannin
Daun Jambu Biji (Psidium guajava L.) sebagai Antikanker Payudara
Get PDFGuava leaves are used as a cytotoxic. Previous research has demonstrated that guava leaves contained 61.71% of quersetin and has potential as an cytotoxic. The aim of this study was to determine the cytotoxic activity of 70% ethanol extract of guava leaves against cancer cells T47D. The method used direct counting (viable cell count) to obtained LC50 value. This study used a test solution with six concentrations that were 130.62; 67.98; 35.98; 18.43; 9.56 and 5 µg/ml. The LC50 value from the 70% ethanol extract of guava leaves was 27.54 µg/ml. The results suggested the extract of guava leaves has cytotoxic activity against T47D cells