Al-Kauniyah: Jurnal Biologi
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Pengujian Toksisitas Akut Oral Dan Dermal pada Biolarvasida Metarhizium anisopliae terhadap Tikus Putih Spraque Dawley
Get PDFAcute oral and dermal toxicity test against white rats was conducted to determine the toxicity and side effects of bio-larvacide (Metarhizium anisopliae crude extract) on humans. In the oral test used a maximum dose 5000 mg/kg and dermal testing used a maximum dose of 2000 mg/kg. Dose treatment and control tested to 5 Spraque Dawley male rats. The results showed that oral treatment with a dose of 5000 mg/kg did not cause mortality and did not cause changes in anatomic pathology of viceral organs. In the dermal treatment with a dose of 2000 mg/kg did not cause mortality and did not cause changes in anatomic pathology of viceral organs. Based on these results LD50 acute oral M. anisopliae biolarvacide above 5000 mg/kg and the acute dermal is above 2000 mg/kg. It was therefore concluded that the formulation of Metarhizium anisopliae biolarvasida classified as not hazardous when used in accordance with the recommendation of the class I (WHO, 2003)
Kadar Protein Klebsiella pneumoniae Hasil Pemanasan 65 Derajat Celcius
No full textKlebsiella pneumoniae is one of a coliform bacteria that causing mastitis. This disease were founded in dairy cows and can be prevented by vaccination. The research has been conducted to determine the inactive times, the protein concentration and profile of K. pneumoniae which inactivated by heating of 65oC as material of mastitis vaccine. The cells culture inactivated by the different times, i.e. 0, 10, 20, 30, 40, 50 and 60 minutes. The inactive times was determined by the drop test method, whereas the protein concentration of cells were determined by Lowry method. The results showed that the inactive times occured after 30 minute, and has a significant different on the protein concentration of bacteria cells that inactivated by the different times
Perbandingan Morfologi Dua Jenis Anggrek Epifit pada Pohon Rambutan ( Nephelium lappaceum L.) : Acriopsis liliifolia (J.Koenig) Seidenf. dan Dendrobium crumenatum Sw.
Get PDFThe stem of rambutan tree (Nephelium lappaceum) has suitable microenvironment for the growth of epiphytic flora, due to its rugose stem. Therefore, it may trap and collect plant debrish or other organic material as well as rain drops. One of plant group found on the rambutan tree is orchid (Orchidaceae). This study aimed to observed the morphology of epihytic orchids on rambutan tree. The result showed two orchid species, Acriopsisliliifolia (J. Koenig) Seidenf. and Dendrobium crumenatum Sw
Ekstrak Secang sebagai Bahan Diuretikum (Percobaan terhadap Tikus Putih Jantan Galur Spraque Dawley)
Get PDFEmpirically, secang (Caesalpinia sappan L.) utilized as natural materials to overcome various types of disease but not yet known how the influence of consuming secang against the volume urine. The experiment was conducted on white male rats varian Spraque dawley and sample test extract ethanol 96% of dried bark secang. The animal try grouped in seven groups and each group consists of six animal try, namely normal group, positive group (furosemide 10 mg/200 g BW), negative group (water 2 ml/200 g BW), Group of I dose (62,5 mg/kg BW), dosage II (125 mg/kg BW), dosage III (250 mg/kg BW), and dosage IV (500 mg/kg BW). Thirty minutes after granting test sample, the animal try given drinking water 2 ml/200 g BW by gastric sonde, then put them in metabolite cages for 16 hours (overnight). The result of experiment conducted show the average volume urine of animal try of normaly group was 4,5 ml, negative group was 4,25 ml, dosage I was 4,5 ml, dosage II was 8 ml, Dosage III was 5,75 ml and dosage IV was 5,5 ml. The volume of animal try urine in the group II was the highest, so it can be said that secang extract (dosage II – 125 mg/kg BW) can be used as diureticum material
Produksi Enzim Amilolitik dari Bacillus megaterium Menggunakan Variasi Kadar Pati Sagu (Metroxylon sp.)
Get PDFThe application of enzymes as biocatalysts for the industries in Indonesia has increased. Among the enzymes that are needed in Indonesia, one of which amylolytic enzymes. Amylolytic enzymes constitute a group of enzymes that catalyze the hydrolysis of starch into simple sugars consisting of glucose units. In this study amylolytic enzyme isolated from the bacterium Bacillus megaterium. Enzyme production was submerged fermentation method for 14 hours using sagostarch Metroxylon sp. varies. Measurement of enzyme activity was determined by the method amylolytik Somogy-Nelson. Research results showed that2 % (w/v) of sago starchis the optimum consentration in media with highest amylolytic enzyme activity that is equal to 0.076 units/ml and sugar medium formed by181.254ug/ml in the fermentation medium
Biomassa Bibit Tanaman Jambu Mete (Anacardium occidentale L.) yang Ditanam pada Tanah Pascatambang Emas Bombana dengan Variasi Pupuk Kandang
Get PDFResearch to back up ex gold mining restoration must been done with productive plant so gets to answer about problem environmental one arises mining industry effect and can get to increase economy of plant exploit facet. This research aims was to know plant seed growth Cashew nut (Anacardium occidentale L.) with biomassa accumulation on organ comes to root, bar, and plant seed leaf of Cashew nut ( Anacardium occidentale L.) that old three-month after implant. Experiment research utilize fledged random design (RAL), consisting tree manures (crib manures, goat den manures, and henhouse manures) with same dose, which is 150 ha-1 tons, and control (without manures) with five replicated. Experimental procedure consisting of plant media preparation utilize ex mining land, preparation organic and an organic manure seed instilling, manuring, preserve, and cropping. Instilling and plant preserve is done in green house FMIPA UHO\u27S Biological. Result observationaling to point out that biomassa allocation plant tends to be presented at by leaf then bar organ and root, well on control and also on conduct by manures den. Biomassa allocation percentage in plant organ that is given manures greater crib than deep plant organ on conduct manures henhouse, goat den manure and control. Biomassa allocation most little on organ comes to root with percentage most little on conduct manures crib then on henhouse manure and supreme on goat den manure
Performa Udang Hias Red Cherry (Neocaridina heteropoda) pada Fase Pembesaran Melalui Aplikasi Warna Wadah Berbeda
Get PDFShrimp red cherry shrimp is one of the ornamental potential developed. One of the conservation efforts can be made to increase production by optimizing keragaannya on enlargement phase. This study aims at scaling up the production of red cherry shrimp, especially in the phase of enlargement. Treatment in the form of background color is composed of (A) without colors (control), (B) white, (C) in black, and (D) red. Each treatment was repeated three times with a 45-day long research. Container maintenance in the form of an aquarium measuring 14x14x14 cm3 volume of 1 l and equipped with aeration. The prawns used were 10 fish / aquarium with a weighted average of 0.018 ± 0.21 g and the total length of the average of 0.82 ± 0.21 cm. Feeding in the form of an ad bloodworm satiation and include aquatic plant Hydrilla sp. as a source of additional food and shelter in the form of detritus. The results showed that the weight gain and the highest long background color was achieved by treatment D (red) which is equal to 0.09 ± 0.3 g and 1.62 ± 0.3 cm. Survival at each treatment reached 100%. The water quality of all treatments during the study period remained within normal limits
Perbandingan Pertumbuhan Jamur Tiram Putih di Kumbung Ciseeng dan Universitas Al-Azhar Indonesia
Get PDFWhite oyster mushroom (Pleurotus ostreatus) is one of wood fungi which easy to grow at rotting wood and organic waste. White oyster mushroom is rich in protein, carbohydrates, minerals, and vitamins. Environmental factors affecting the growth of white oyster mushrooms is temperature and humidity. The study aims to compare the growth and production of white oyster mushroom in different location, Ciseeng and University of Al-Azhar Indonesia (UAI). Cultivation in UAI done in a controlled situation using autoclave sterilization, inoculation using enkas and the treatment is watering on gunny sack in the mushroom house regularly. Whereas, cultivation in Ciseeng less controlled by traditional cultivation using steam sterilization and inoculation process at open space. Wet weight, dry weight, and biological efficiency results is higher in UAI than Ciseeng at 7.95%, 14.5%, and 7.34% higher, respectively. Morphological parameter such as number of fruiting body, mushroom cap diameters, and stem lenght also show better in UAI than Ciseeng at 23.67%, 8.75%, 54.96% higher, respectively. Better result of mushroom cultivation at UAI supported by 28.3oC average temperature and 93.95% humidity, meanwhile in Ciseeng average temperature and humidity was 29.25oC and 82.15%
Pengurangan Kadar N dan P pada Limbah Cair Laboratorium secara Fitoremediasi Menggunakan Enceng Gondok (Eichornia crassipes)
Get PDFLaboratory liquid waste contains both metal, nonmetal, organic and anorganic content. If it is discharged directly to environment, it will cause pollution that is harmful for living thing. Data of waste survey result in 2009-2010 indicated that liquid waste of Laboratory in Chemical Engineering Department, Gadjah Mada University (JTK UGM) consist of various complex compound such as NH4+, PO43-. JTK UGM has wastewater treatment plant (WWTP) to deal with environmental pollution coming from waste of each laboratory and have three process, anaerobe activated sludge, aerobe and phytoremediation. Because the laboratory’s waste was very complex, the WWTP have not operated maximally yet. So, it is necessary research on wwtp processing step, particularly for phytoremediation process using water hyacinth. Objective of this research was to study effect of concentrations N and P on adaptability and capability of water hyacinth to grow. In addition it developed a mathematical model to estimate of N and P concentration decrease rate in liquid. The research was done in batch by planting water hyacinth that has been acclimated in wastewater containing N-NH4OH and P-H3PO4. Parameter observed is N and P concentration in liquid. The result indicated that water hyacinth can absorb N and P. Water hyacinth grow well in N-NH4OH concentration of 9.651 ppm and 15.573 ppm and P-H3PO4 concentration of 2.013 ppm and 2.7373 ppm. It was marked with additional weight and colour of water hyacinth leaves of fresh green. Mathematical model presented to estimate N concentration decrease rate indicated good result in experiment. However, P concentration decrease in liquid phase, the presented model is still not good, because P nutrient decomposition process has not been accommodated during absorption by water hyacinth
Jenis dan Status Konservasi Ikan Hiu yang Tertangkap di Tempat Pelelangan Ikan (TPI) Labuan Bajo, Manggarai Barat, Flores
Get PDFThe study aimed to determine the types of sharks are caught, sold and conservation status in the fish auction place (TPI) Labuan Bajo, West Manggarai, Flores, East Nusa Tenggara have been conducted in February and March 2014 in Labuan Bajo. The research method was the survey method and used Market Survey sampling techniques and identification by Rapid Assessment method. Descriptively analyzed data, displayed in the form of narration and a table. Based on the results of the study, found 114 individual sharks, 55 individual males, 59 females were classified into three orders, namely Orectolobiformes, Lamniformes, and Carcharhiniformes; 4 families that Carcharhinidae, Odontaspididae, Rhynchobatidae, and Ginglymostomatidae; 5 genera namely Carcharhinus, Triaenodon, Rhyncobatus, Prionace, and Rhizoprionodon and 9 types of sharks that Carcharhinus melanopterus, Triaenodon obesus, Carcharhinus limbatus, Carcharhinus taurus, Carcharhinus amblyrhynchos, Rhynchobatus australiae, Prionace glauca, Rhizoprionodon acutus, and Nebrius ferrugineus. Based on the state of conservation, shark found in Labuan Bajo TPI classified Vulnerable and Near Threatened, the dominant type of shark found is Carcharhinus melanopterus (91 fishes) while the least was found is Rhynchobatus australiae, Prionace glauca, Rhizoprionodon acutus, and Nebrius ferrugineus (each 1 individual)