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    Effect of yerba mate ( Ilex paraguariensis A. St.-Hil.) supplementation on oxidative stress in ruminants

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    Nutrition, especially antioxidant-rich herbal supplementation, is one of the most important factors in the reaction to oxidative stress. Moreover, it influences animal productivity and reproduction. When oxidant production exceeds the capacity of antioxidant defense, this results in oxidative damage to lipids, DNA, and proteins. Oxidative stress, which is described as an imbalance between oxidant and antioxidant levels, is a current field of research in ruminant medicine, and it has been involved in numerous disease processes, including sepsis, mastitis, acidosis, and ketosis. Interest in plant extracts has therefore increased due to the high amount of natural biologically active substances that are potential natural fodder additives. This paper focused on yerba mate tea, an infusion made from the leaves of the Ilex paraguariensis A. St.-Hil. tree, which is a widely consumed traditional beverage in South America. There is growing interest for the properties of yerba mate. Supplementation of I. paraguariensis for large and small ruminants resulted in lower oxidative stress and also improved their productive and reproductive performance

    Latex quality and yield parameters of Hevea brasiliensis (Willd. ex A. Juss.) Mu\u308ll. Arg. clone PB 260 for different tapping and stimulant application frequencies

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    Tapping and stimulant applications are an attempt to increase latex yield. A combination of stimulants with low intensity tapping systems was expected to reduce tapping costs. In addition, it was necessary to regulate the stimulant application frequency based on type and potential clone production. The objective was to determine the effect on physiological parameters and latex yield of Hevea brasiliensis (Willd. ex A. Juss.) Mu\u308ll. Arg. clone PB 260 for different tapping and stimulant application frequencies. The study design was split block in randomized complete block plots with three replicates and two factors. The main plot was the tapping system consisting of four levels: quarter-spiral downward tapping (S/4 d3), quarter-spiral upward tapping (S/4U d3), half-spiral downward tapping (S/2 d3), and half-spiral upward tapping (S/2U d3). The subplots were four stimulant application frequencies consisting of ethephon 2.5% applied once every 15 d (ET 15d) and gas stimulant (equal to \ub1 100% ethylene) applied once every 9 d (ETG 9d), once every 18 d (ETG 18d), and once every 27 d (ETG 27d). The highest sucrose levels (3.62 mM) were obtained in S/2 d3 and ET/15d combination. The highest inorganic P content (27.68 mM) was in S/2 d3 and ETG/18d. The highest yield (2418.53 kg ha-1 yr-1) was in S/4d3 and ETG/18d. The tapping system S/4d3 and ETG/18d combination was able to increase production by 102.26% compared with the conventional tapping system (S/2 d3 and ET/15d). After the 1-yr trial, all treatments showed 0.47 to 0.54 mM thiol content, which indicated that plants did not experience over-exploitation stress

    Community composition of rhizosphere fungi as affected by Funneliformis mosseae in soybean continuous cropping soil during seedling period

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    Arbuscular mycorrhizal (AM) fungi can enhance plant resistance particularly against soil-borne pathogenic fungi. However, little is known about the effects of Funneliformis mosseae on the community composition of rhizosphere fungi in soybean ( Glycine max [L.] Merr.) continuous cropping soil. Here, the disease index of soybean root rot was analyzed, and high throughput sequencing technology was applied to investigate whether F. mosseae could change the composition of fungal communities in the rhizosphere of continuous cropping soybean during seedling period. The results indicated that the disease index of soybean root rot decreased significantly after inoculation of F. mosseae. The root rot disease index was also affected by the increasing of continuous cropping regimes. Furthermore, the relative abundance of fungal community in soybean rhizosphere soil and root samples was influenced after inoculation. Ascomycota was the dominant phylum in most samples. Basidiomycota was the second dominant phylum in all the soil samples, but Olpidiomycota was the second phylum in most root samples. At the genus level, both inoculation and continuous cropping regimes had significant effects on the dominant genus and their relative abundances in all the samples. The relative abundance of some plant pathogenic fungi such as Fusarium in the inoculated root samples was lower than those in the non-inoculated root samples in the same continuous cropping regime. The results can provide new insights into the interactive effects of AM fungi and rhizosphere fungi, and also provide theoretical evidence on biological solutions to alleviate the obstacles of soybean continuous cropping

    Stay green physiological capacity of drought tolerant maize inbred lines

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    Stay green is the ability of a plant to maintain photosynthetically functional green leaf area for longer periods, even under excessive water stress. This study was done to establish the physiological capacity of Ugandan drought tolerant maize inbred lines to stay green under water shortage conditions using various stay green physiological determinants. Seventy-six maize inbred lines characterised for drought tolerance by CIMMYT, were planted under a rain out shelter, with a well-watered control (WW) and excessive water-stress treatments applied at six weeks after planting (6 WAP) and eight weeks after planting (8 WAP). The most maintained stay green physiological determinants under excessive water-stress were non-photosynthetic quench (NPQt) and leaf pigmentation per unit leaf area (RC: LAUG) with inbred lines CELQ15028 and CELQ15022 showing the highest maintenance for the two parameters, respectively. The highest level of variation for quantum yield of photosystem II activity (Phi2) and NPQt among inbred lines was at 40 and 60 days after flowering, respectively. Phi2 was negatively correlated to NPQt but positively correlated to the linear electron flow (LEF); while NPQt had a significantly negative correlation with LEF. This study established that these drought tolerant maize inbred lines have moderate stay green physiological capacity, with inbred line CEL15027 performing the best.Le retard de s\ue9nescence du feuillage (\u201cstay green\u201d) est la capacit\ue9 d\u2019une plante \ue0 maintenir une surface foliaire verte photosynth\ue9tiquement fonctionnelle pendant de plus longues p\ue9riodes, m\ueame en cas de stress hydrique excessif. Cette \ue9tude a \ue9t\ue9 r\ue9alis\ue9e pour \ue9tablir la capacit\ue9 physiologique des lign\ue9es consanguines de ma\uefs ougandaises tol\ue9rantes \ue0 la s\ue9cheresse \ue0 retard de s\ue9nescence du feuillage (\u201cstay green\u201d) dans des conditions de p\ue9nurie d\u2019eau en utilisant divers d\ue9terminants physiologiques de retard de s\ue9nescence du feuillage (\u201cstay green\u201d). Soixante-seize lign\ue9es consanguines de ma\uefs caract\ue9ris\ue9es pour la tol\ue9rance \ue0 la s\ue9cheresse par le CIMMYT ont \ue9t\ue9 plant\ue9es sous un abri anti-pluie, avec un contr\uf4le bien arros\ue9 (WW) et des traitements contre le stress hydrique excessif appliqu\ue9s six semaines apr\ue8s la plantation (6 WAP) et huit semaines apr\ue8s la plantation. (8 WAP). Les d\ue9terminants physiologiques verts de s\ue9jour les plus maintenus sous un stress hydrique excessif \ue9taient la trempe non photosynth\ue9tique (NPQt) et la pigmentation des feuilles par unit\ue9 de surface foliaire (RC: LAUG) avec les lign\ue9es consanguines CELQ15028 et CELQ15022 pr\ue9sentant le maintien le plus \ue9lev\ue9 pour les deux param\ue8tres respectivement. Le niveau de variation le plus \ue9lev\ue9 du rendement quantique de l\u2019activit\ue9 du photosyst\ue8me II (Phi2) et du NPQt parmi les lign\ue9es consanguines \ue9tait respectivement \ue0 40 et 60 jours apr\ue8s la floraison. Phi2 \ue9tait corr\ue9l\ue9 n\ue9gativement au NPQt mais positivement corr\ue9l\ue9 au flux d\u2019\ue9lectrons lin\ue9aire (LEF) tandis que NPQt avait une corr\ue9lation significativement n\ue9gative avec le LEF. Cette \ue9tude a \ue9tabli que ces lign\ue9es consanguines de ma\uefs tol\ue9rantes \ue0 la s\ue9cheresse avaient une capacit\ue9 physiologique \ue0 retard de s\ue9nescence du feuillage (\u201cstay green\u201d) mod\ue9r\ue9e, la lign\ue9e consanguine CEL15027 a mieux fonctionn\ue9

    Biosorption of Zn (II) ions from Aqueous Solution by Immobilized Aspergillus fumigatus

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    The objective of this study was to investigate the biosorption of Zn (II) from aqueous solution by Aspergillus fumigatus immobilized in calcium alginate. The effects of adsorbent dosage, initial solution pH, contact time and initial Zn (II) concentrations were investigated. Results were fitted to the Langmuir isotherm. The results showed an increase in biosorption efficiency with increase in biosorbent dosage. The optimum pH of adsorption was 5.0 while the maximum adsorption was achieved within 10 minutes at initial Zn (II) concentration of 1 mg/L. The experimental results showed a high R2 (0.9070) value for the Langmuir isotherm. This therefore suggests that it is a monolayer adsorption. The maximum biosorption capacity was 3.55 mgg-1. These results indicate that zinc metal removal by biomass of Aspergillus fumigatus immobilized in alginate is a low cost wastewater treatment option and can be effectively used in small scale treatment plants

    Protective Function of Butylated Hydroxytoluene in Lead-Induced Oxidative Alterations in Tissues of Wistar Rats

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    This study was designed to evaluate the protective function of butylated hydroxytoluene (BHT) in Pb-induced oxidative damage to blood, hepatic and renal tissues of exposed rats. Four groups (I-IV) with six male Wistar rats each, were considered in this study, where group I rats (control) received 1 ml of corn oil, group II receive intraperitoneally 30 mg PbCl2/kg b. wt of rats only, group III rats were administered with NOAEL of BHT at 25 mg/kg and group IV rats were administered intraperitoneal injection of 30 mg PbCl2 plus oral administration of 25 mg BHT dispersed in corn oil per kg b. wt of the rats for 28 days with the evaluation of oxidative stress biomarkers in the tissues. Pb significantly increased tissue MDA levels in group II rats, but BHT reduced MDA levels in tissues for groups III and IV rats at p<0.05 relative to control. In serum, biomarkers (GSH: 1.46 \ub1 0.08 \u3bcg/mg protein; CAT: 63.74 \ub1 0.20 nits/mg protein; SOD: 16.49 \ub1 0.39 units/mg protein and GPx: 12.84 \ub1 0.17 units/mg protein) exhibited significant reduction (p<0.05) in group II rats, but were significantly stimulated in groups III and IV rats relative to group I rats. Similar trends were observed in hepatic and renal tissues. Therefore, the present study successfully established the functional role of BHT in the amelioration of Pb-induced oxidative stress in exposed rats

    Occurrence of Acetaminophen, Amoxicillin, Diclofenac and Methylparaben in Lagos and Ologe Lagoons, Lagos, Nigeria

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    The occurrence of acetaminophen, amoxicillin, diclofenac and methylparaben in Lagos and Ologe Lagoons was investigated by random sampling of the water bodies during rainy and dry seasons. Samples analysis was carried out using high performance liquid chromatography. For the two seasons, the mean environmental concentrations of acetaminophen in Lagos and Ologe Lagoons were 0.075 \u3bcg/L and 1.233 \u3bcg/L respectively. The mean environmental concentration of amoxicillin in Lagos Lagoon was 0.008 \u3bcg/L while 0.003 \u3bcg/L was the mean value in Ologe Lagoon. Diclofenac occurrence in Lagos Lagoon had a mean value of 0.136 \u3bcg/L while 0.519 \u3bcg/L was recorded in Ologe Lagoon. The environmental concentration of methylparaben in Lagos Lagoon was 0.453 \u3bcg/L while its means concentration in Ologe Lagoon was 0.089 \u3bcg/L. From the results, the environmental concentrations of acetaminophen and diclofenac in Ologe Lagoon were higher than their level of occurrence in Lagos Lagoon. However, the levels of occurrence of amoxicillin and methylparaben in Lagos Lagoon were higher than their environmental concentrations in Ologe Lagoon. Methylparaben was the most frequently detected in the water bodies with 87.5% detection frequency. This was followed by diclofenac with 75%. Also, 50% of the water samples analysed contained acetaminophen while amoxicillin had the least (37.5%) frequency of occurrence. The study established that there are active pharmaceuticals compounds and excipients present in the Nigerian aquatic environment which are micropollutants of emerging environmental concerns and of significant ecological risk. Therefore, there is need for environmental regulators to make concerted efforts towards environmental monitoring of this group of pollutants

    Assessment of Physicochemical Properties and Heavy Metals in Borehole Water Used For Drinking In Okada Town, Edo State, Nigeria

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    The consumption of unsafe water is detrimental to human health. It is therefore important to ascertain the quality and purity of water set out for drinking. This paper investigates the physiochemical properties of drinking water obtained from boreholes located at various sites in Okada town, Nigeria. The assessment of physicochemical properties and heavy metal contents of groundwater (borehole) quality were evaluated. Ten water samples labeled A-J were collected from boreholes at different locations in Okada community and subjected to laboratory test to determine the pH, biological oxygen demand (BOD), chemical oxygen demand (COD), dissolved oxygen (DO), hardness, turbidity, total organic carbon, total dissolved solids, electrical conductivity, and concentration of phosphates, nitrates, lead, sodium, calcium and chloride. The pH in the water samples is an indication of acidity and the WHO standard for pH value is specified within the range of 6.5-8.5. However the pH values obtained for the samples are A(4.37), B(4.44), C(5.06), D(4.86), E(6.40), F(4.25), G(5.95), H(4.53 ), I(6.61) and J(5.70). This implies that only the sample I of pH value 6.61 falls within the WHO standard and safe for drinking. Thus, the water from the community can only be considered safe to drink except a neutralizing filter is incorporated into the water system in other to avert the implications of consuming acidic water

    Vertical Electric Sounding of Leachate Contaminant Plumes at a Dumpsite in Obigbo, Rivers State, Nigeria

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    The quality of underground water at a dumpsite in Obigbo, Rivers State, Nigeria, was investigated by collecting 7 vertical electrical soundings (VES) using Wenner electrode configuration with current electrode spacing (AB/2) ranging from 1.0 m to 300 m. Field data were acquired using the SAS 300C resistivity meter and accessories, coordinates and elevation were measured using the Global Positioning System (GPS) at each sounding station. The VES data showed that the area is composed of clay, sandy clay and sand. The contaminated zones have low resistivity and corresponding high conductivity. Two zones were identified, zones of low resistivity and high resistivity with values of 11.3 \u3a9m to 21.4 \u3a9m, and 357.0 \u3a9m to795.0 \u3a9m respectively. There is thus, both lateral and downward movement of the contaminant leachate plumes. The results also revealed that the surrounding soil and groundwater around the landfill has not been contaminated to depths exceeding 15.6 m, which is shallower than the productive aquifer depth greater than 37.0 m. Judging from the age of the dumpsite, this site has a good protecting capacity, probably as a result of the presence of sufficient confined layers of clay that have impeded or slowed down the percolation of leachate into the aquifer. The aquifer is the underlying rocks of sand and gravel that are porous which constitute the water body

    Assessment of Radionuclide Concentration in Surface Soil and Human Health Risk Associated with Exposure in Two Higher Institutions of Esan land, Edo State, Nigeria

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    The aim of this study is to assess the activity concentration of radionuclides and the possible health risk associated with exposure to radiation from the soil samples collected from two higher institutions in Esan land of Edo State, Nigeria. The gamma spectrometry system was used to determine the activity concentrations of radionuclides 238U, 232Th and 40K in the surface soil samples. The activity concentration of radionuclides ranged from 7.16 \ub1 0.63 to 102.37 \ub1 2.37 BqKg\u20141 for 40K, from 0.75 \ub1 0.05 to 3.13 \ub1 0.11 BqKg\u20141 for 238U and from 2.25 \ub1 0.20 to 9.02 \ub1 0.40 BqKg\u20141 for 232Th. The measured mean activity concentration of radionuclides for 40K, 238U and 232Th from the examined topsoil of Samuel Adegboyega University were 57.80 \ub1 1.7 BqKg\u20141; 2.07 \ub1 0.09 BqKg\u20141 and 6.89 \ub1 0.34 BqKg\u20141 respectively. At the neighbouring higher institution; College of Education, Igueben, the mean activity concentration of 40K, 238U and 232Th in its surface soil were 30.19 \ub1 1.22 BqKg\u20141, 1.41 \ub1 0.07 BqKg\u20141 and 4.85 \ub1 0.28 BqKg\u20141 respectively. The total mean values for radiation dose estimation for Radium Equivalent Activity, Absorbed Dose, Excess Lifetime Cancer Risk, Outdoor and Indoor Annual Effective Dose Equivalent are 13.20 BqKg\u20141, 6.03 nGy/\u210e, 0.026, 7.40 \u3bcSv/y, and 29.60 \u3bcSv/y respectively. These values were found to be lower than the global average values. Hence, there is no radiation induced health threat to the students and members of staff in the study area

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