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    CASSAVA MOSAIC DISEASE INCIDENCE AND SEVERITY AND WHITEFLY VECTOR DISTRIBUTION IN GABON

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    Cassava mosaic disease (CMD) is a major constraint to cassava ( Manihot esculenta Crantz) production in sub-Saharan Africa. The objective of this study was to gain insights into the epidemiology of CMD in cassava production systems in Gabon. An epidemiological survey was conducted throughout Gabon from October 2020 to May 2021 to evaluate the epidemiology of cassava mosaic disease (CMD) in cassava farms. A phytosanitary diagnosis was conducted at each farm, using the harmonised and unified WAVE protocol to assess the incidence and severity of CMD and the abundance of its whitefly vector. All data collected were recorded using WAVE\u2019s mobile application and uploaded into the WAVE Cube data system. The results showed that CMD was present in all farms surveyed (n = 227; 100%), but with a variable incidence rate. At national level, the incidence of CMD was high, with an average of 64.29%. However, incidence differed among regions with 82.22 and 79.20% for Ogooue-Maritime and Nyanga; respectively, but only 40.42% for Estuaire. Woleu-Ntem had the highest mean severity (3.47) and Ogooue-Maritime the lowest (2.64). Overall, incidence and severity differed significantly among regions (P< 0.05). The average abundance was 2.58 whiteflies per plant (w/p), varying between 1.06 (Nyanga) and 5.25 w/p (Estuaire). The use of infected cuttings was the main mode of CMD spread (62.67%). These results highlight the need to identify the viruses responsible for the observed cases of CMD, to sanitise cultivated plant material, and to implement a CMD control strategy in Gabon.La maladie de la mosa\uefque du manioc (CMD) est une contrainte majeure \ue0 la production de manioc (Manihot esculenta Crantz) en Afrique subsaharienne. L\u2019objectif de cette \ue9tude \ue9tait de mieux comprendre l\u2019\ue9pid\ue9miologie de la CMD dans les syst\ue8mes de production de manioc au Gabon. Une enqu\ueate \ue9pid\ue9miologique a \ue9t\ue9 men\ue9e dans tout le Gabon d\u2019Octobre 2020 \ue0 Mai 2021 afin de mieux connaitre l\u2019\ue9pid\ue9miologie de la mosa\uefque du manioc. Un diagnostic phytosanitaire a \ue9t\ue9 fait dans chaque champ prospect\ue9. L\u2019incidence, la s\ue9v\ue9rit\ue9 de la mosa\uefque et l\u2019abondance des mouches blanches vectrices ont \ue9t\ue9 estim\ue9es en utilisant le protocole harmonis\ue9 et unifi\ue9 du programme WAVE. Toutes les donn\ue9es de terrain ont \ue9t\ue9 saisies gr\ue2ce \ue0 l\u2019application mobile de WAVE puis stock\ue9es dans le gestionnaire de donn\ue9es de WAVE, le Cube. Les r\ue9sultats de cette \ue9tude ont montr\ue9 que la mosa\uefque du manioc \ue9tait pr\ue9sente dans toutes les zones prospect\ue9es (n = 227; 100%), avec une incidence variable. Au niveau national, l\u2019incidence de la CMD \ue9tait \ue9lev\ue9e, avec une moyenne de 64,29% pour une s\ue9v\ue9rit\ue9 moyenne de 3,16. Cette incidence varie entre les provinces avec respectivement 82,22% et 79,20% pour l\u2019Ogoou\ue9-Maritime et la Nyanga, mais seulement, 40,42% pour l\u2019Estuaire. La r\ue9gion du Woleu-Ntem a pr\ue9sent\ue9 la plus grande s\ue9v\ue9rit\ue9 moyenne (3,47) et l\u2019Ogoou\ue9-Maritime, la plus faible moyenne (2,64). L\u2019incidence et la s\ue9v\ue9rit\ue9 ont diff\ue9r\ue9 significativement entre les r\ue9gions (P< 0,05). L\u2019abondance moyenne des mouches blanches \ue9tait de 2,58 mouches/plante (m/p). Cette abondance a oscill\ue9 entre 1,06 m/p (Nyanga) et 5,25 m/p (Estuaire). L\u2019utilisation des boutures de manioc infect\ue9es est le principal mode de propagation de la CMD (62,67%). Ces r\ue9sultats pr\ue9liminaires mettent en exergue la n\ue9cessit\ue9 imp\ue9rieuse d\u2019identifier les virus responsables des cas de CMD observ\ue9s, d\u2019assainir le mat\ue9riel v\ue9g\ue9tal cultiv\ue9 et de mettre en place une strat\ue9gie de contr\uf4le de la CMD au Gabon

    Comparing durum wheat cultivars by genotype 7 yield 7 trait and genotype 7 trait biplot method

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    The specification of the most convenient cultivars based on multiple trait indices is a new approach in durum wheat ( Triticum durum Desf.) adaptation and stability studies. This approach helps to define the best cultivar based on multiple traits and multiple locations because cultivars are affected by unpredictable climatic conditions. Some traits (ears per square meter, spike length, number of grains per spike, spike yield, and leaf chlorophyll content among others) can be produced for primary breeding purposes because they are influenced by environmental factors and indirectly affect grain yield and quality. Therefore, in the present study, the new genotype 7 yield 7 trait (GYT) biplot approach was used to identify the best cultivar among 10 durum wheat cultivars based on multiple environments (8) and multiple traits (18). Cultivar ranking was examined by a superiority index that combined yield and other target traits with the GYT biplot. The general adaptability of each cultivar in terms of all the traits indicated differences based on environment means, and significant differences were found between varieties for the GYT biplot. In the GYT biplot, yield-trait combinations clearly indicated the most stable cultivars, whereas in the genotype 7 trait (GT) biplot, the best cultivars were not defined for all traits. \u2018Sari\ue7anak\u2019 was ranked as the best combination of physio-morphological traits with grain yield, \u2018Z\ufchre\u2019 was the best for more quality traits, and \u2018G\ufcneyyildizi\u2019 was the best for both physio-morphological and quality traits in the GYT biplot. The GYT biplot combines traits with yield and can help the visual identification of the best cultivars; it is better than the GT biplot method

    Transcriptome assembly and expression analysis in Colletotrichum gloeosporioides -tolerant Rubus glaucus Benth.

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    Andean blackberry (Rubus glaucus Benth.) is an important crop of the Andean region affected by Colletotrichum gloeosporioides. In Colombia, tolerant plant material has been detected, but it has not been completely characterized. The objective of this research was oriented to analyze de novo transcriptome assembly of R. glaucus, and the comparison of the assembly with different reference genomes to further complete differential expression analysis of R. glaucus tolerant to C. gloespoiorides attack. To achieve this, three groups were used: infected tolerant material, infected susceptible material, and a susceptible group without inoculation. The RNA-seq sequencing was achieved through Illumina Hi-seq 2000. De novo assembly (Trinity, CD-HIT, TopHat) and functional annotation of sequences were carried out, additionally, mapping with reference genomes belonging to Rosaceae families was conducted (Bowtie2, TopHat). Subsequently, the differential expression was quantified (Cuffdiff) and analyzed through EdgeR. Variant analysis was made using MISA and SAMtools. After editing and assembly, 43579 consensus sequences were obtained (N50 = 489 bp; GC = 44.6%), annotation detected 35824 and 35602 sequences in Nt (partially non-redundant nucleotide sequences) and Nr (non-redundant protein sequences) databases, respectively. The 85% of Nr sequences was linked to members of Rosaceae family, mainly strawberry (67.6%). A total of 3570 simple sequence repeat (SSR) markers and 38791 single nucleotide polymorphisms (SNP) were found. The transcriptome of tolerant plants exhibited less SNPs. Finally, differentially expressed genes were found, including RPM1, MAPKBP1, CKX2. This research represents a contribution for future understanding of R. glaucus transcriptome, since there is little information available, and it would help further tolerance-related analysis

    Distribution and molecular characterization of Citrus yellow vein clearing virus in Yunnan Province of China

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    In 2009, a new citrus viral disease caused by Citrus yellow vein clearing virus (CYVCV) was first discovered in Yunnan province of China. In this study, a survey was conducted in 27 orchards from Yunnan province from April 2017 to September 2018. In all, 45 of a total of 513 citrus samples were tested positive for CYVCV by reverse transcription polymerase chain reaction (RT-PCR). Furthermore, the complete genome sequences of six CYVCV isolates from different hosts were sequenced. Comparisons of the whole genome sequences of these six CYVCV isolates as well as 34 isolates previously reported from around the world revealed the sequence identity ranged from 96.9% to 99.8% at nucleotide level, indicating that there is a very low level of sequence heterogeneity among CYVCV isolates of different hosts in Yunnan province

    PREVALENCE OF PLANT-PARASITIC NEMATODES IN RICE FIELDS IN GHANA

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    Rice ( Oryza sativa L.) is one of the most cultivated crops worldwide whose production in sub-Saharan Africa is extensively affected by root nematodes. The objective of this study was to identify and establish the distribution of plant-parasitic nematodes (PPNs), in rice growing fields within different regions of Ghana. Soil and root samples were taken from rice fields, namely fourteen from Volta Region, eleven from the Soil and Irrigation Research Centre (SIREC), Kpong (Eastern Region); and five from Dawhenya (Greater Accra Region). The soil samples were taken from 0-30 cm depths, together with root samples. A total of twenty-four nematode genera were identified in soil and root samples from the three regions. These included: Aphelenchus spp., Belonolaimus spp., Ditylenchus spp., Dolichodorus spp., Helicotylenchus spp., Hemicriconemoides spp. Hemicycliophora spp. Heterodera spp., Hirschmaniella spp., Hoplolaimus spp., Longidorus spp., Meloidogyne spp., Paralongidorous spp., Paratylenchus spp., Pratylenchus spp., Radopholus spp., Rhabditida spp., Rotylenchulus spp., Scutellonema spp., Trichodorus spp., Tylenchornchus spp., Tylenchulus spp., Tylenchus spp. and Xiphinema spp. In the Volta Region, Tylenchus spp. was the most abundant (29.01%) in the soil; while Meloidogyne spp. was most the abundant (36.86%) in the roots. In Dawhenya regions, Meloidogyne spp. was the most abundant (26.96%) in the soil; while Tylenchus spp. was the most abundant (25.94%) in the roots. In the Eastern Region, Meloidogyne spp. was the most abundant (41.7%) in the soil; while Pratylenchus spp. was the most abundant (36.1%) in the roots. These nematodes threaten rice production in Ghana, if not managed well in farmer\u2019s fields.Le riz ( Oryza sativa L.) est l\u2019une des cultures les plus cultiv\ue9es au monde dont la production en Afrique subsaharienne est largement affect\ue9e par les n\ue9matodes des racines. L\u2019objectif de cette \ue9tude \ue9tait d\u2019identifier et d\u2019\ue9tablir la r\ue9partition des n\ue9matodes phytoparasites (NPP) dans les rizi\ue8res de diff\ue9rentes r\ue9gions du Ghana. Des \ue9chantillons de sol et de racines ont \ue9t\ue9 pr\ue9lev\ue9s dans les rizi\ue8res, \ue0 savoir quatorze de la r\ue9gion de la Volta, onze du Centre de Recherche sur les Sols et l\u2019Irrigation (SIREC), Kpong (r\ue9gion de l\u2019Est)\ua0; et cinq de Dawhenya (r\ue9gion du Grand Accra). Les \ue9chantillons de sol ont \ue9t\ue9 pr\ue9lev\ue9s \ue0 des profondeurs de 0 \ue0 30 cm, ainsi que des \ue9chantillons de racines. Au total, vingt-quatre genres de n\ue9matodes ont \ue9t\ue9 identifi\ue9s dans des \ue9chantillons de sol et de racines des trois r\ue9gions. Ceux-ci comprenaient : Aphelenchus spp., Belonolaimus spp., Ditylenchus spp., Dolichodorus spp., Helicotylenchus spp., Hemicriconemoides spp. Hemicycliophora spp. Heterodera spp., Hirschmaniella spp., Hoplolaimus spp., Longidorus spp., Meloidogyne spp., Paralongidorous spp., Paratylenchus spp., Pratylenchus spp., Radopholus spp., Rhabditida spp., T. ., Tylenchornchus spp., Tylenchulus spp., Tylenchus spp. et Xiphinema spp. Dans la r\ue9gion de la Volta, Tylenchus spp. \ue9tait le plus abondant (29,01 %) dans le sol; tandis que Meloidogyne spp. \ue9tait le plus abondant (36,86%) dans les racines. Dans les r\ue9gions de Dawhenya, Meloidogyne spp. \ue9tait le plus abondant (26,96 %) dans le sol; tandis que Tylenchus spp. \ue9tait le plus abondant (25,94 %) dans les racines. Dans la r\ue9gion orientale, Meloidogyne spp. \ue9tait le plus abondant (41,7%) dans le sol; tandis que Pratylenchus spp. \ue9tait le plus abondant (36,1 %) dans les racines. Ces n\ue9matodes menacent la production de riz au Ghana, s\u2019ils ne sont pas bien g\ue9r\ue9s dans les champs des agriculteurs

    Fodder yield and nutritive quality of haulm from dual-purpose cowpea cultivars for dry season livestock feeding in Nigeria

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    The availability of high quality forage, especially during the dry season remains a major challenge to ruminant livestock production in Nigeria and many parts of West Africa. This study was conducted to evaluate forage yield and nutritive quality of haulm from selected dual purpose cowpea ( Vigna unguiculata L. Walp.) cultivars in the humid rain forest zone of Nigeria. The forage and pod yields of twenty cultivars were evaluated at the Teaching and Research Farm of the Federal University of Technology, Owerri, Imo State, Nigeria. Subsequently, five dual-purpose cultivars, namely IT04K-334-2, IT07K-293-3, IT04K-194-3, IT04K-405-5, IT06K-147-2, were selected based on forage and pod yields from previous trial, for determination of chemical and fibre compositions. The results revealed significant (P<0.05) differences in pod and forage yields among the twenty cowpea cultivars, with yields ranging from 0-1.5 t ha-1 and 0.1-4.5 t ha-1 for pod and forage, respectively. Cultivars IT07K-293-3, IT04K-405-5, IT06K-147-2, IT07K-194-3, IT04K-334-2, IT04K-267-8 and IT04K-339-1 recorded the highest fodder yields of at least 2715 t ha-1, with crude protein contents ranging from 10.49% in IT07K-194-3 to 13 .57 % in IT04K-405-5. Cultivars IT07K-194-3, IT07K-293-3, IT07K-220-1-9, IT06K-147-2, IT07K-187-55IT04K-332-1 recorded the highest pod yields (>700 t ha-1). There were significant (P<0.05) differences in crude protein, ether extract and non-fibre carbohydrates (NFC) contents among five cultivars selected from the top ten high yielding cultivars, based on mean ranking score. These selected cultivars are recommended as dual-purpose cowpea for the humid forest zone.La disponibilit\ue9 de fourrage de la haute qualit\ue9, en particulier pendant la saison s\ue8che, reste un d\ue9fi majeur pour l\u2019\ue9levage de ruminants au Nigeria et dans de nombreuses r\ue9gions d\u2019Afrique de l\u2019Ouest. Cette \ue9tude a \ue9t\ue9 men\ue9e pour \ue9valuer le rendement de fourrage et la qualit\ue9 nutritive des fanes de cultivars s\ue9lectionn\ue9s de ni\ue9b\ue9 \ue0 double usage ( Vigna unguiculata L. Walp.) dans la zone de for\ueat tropicale humide du Nigeria. Les rendements en fourrage et en gousse de vingt cultivars ont \ue9t\ue9 \ue9valu\ue9s \ue0 la Ferme d\u2019Enseignement et de Recherche de l\u2019Universit\ue9 F\ue9d\ue9rale de Technologie, Owerri, \uc9tat d\u2019Imo, Nig\ue9ria. Les cinq cultivars \ue0 double usage \ue0 savoir IT04K-334-2, IT07K-293-3, IT04K-194-3, IT04K-405-5, IT06K-147-2 ont \ue9t\ue9 s\ue9lectionn\ue9s sur la base des rendements en fourrage et en gousse des essais pr\ue9c\ue9dents pour la d\ue9termination des compositions chimiques et des fibres. Les r\ue9sultats ont r\ue9v\ue9l\ue9 des diff\ue9rences significatives (P<0,05) dans les rendements des gousses et du fourrage parmi les vingt cultivars de ni\ue9b\ue9, avec des rendements allant de 0-1,5 t ha-1 et 0,1-4,5 t ha-1 pour les gousses et le fourrage respectivement. Les cultivars IT07K-293-3, IT04K-405-5, IT06K-147-2, IT07K-194-3, IT04K-334-2, IT04K-267-8 et IT04K-339-1 ont enregistr\ue9 les rendements fourragers les plus \ue9lev\ue9s d\u2019au moins 2715 t ha-1 avec des teneurs en prot\ue9ines brutes allant de 10,49 % en IT07K-194-3 \ue0 13,57 % en IT04K-405-5. Les cultivars IT07K-194-3, IT07K-293-3, IT07K-220-1-9, IT06K-147-2, IT07K-187-55IT04K-332-1 ont enregistr\ue9 les rendements en gousses les plus \ue9lev\ue9s (>700 t ha-1). Il y avait des diff\ue9rences significatives (P<0,05) dans les teneurs en prot\ue9ines brutes, en extraits \ue9th\ue9r\ue9s et en glucides non fibreux (GNF) parmi cinq cultivars s\ue9lectionn\ue9s parmi les dix premiers cultivars \ue0 haut rendement, sur la base du score de classement moyen. Ces cultivars s\ue9lectionn\ue9s sont recommand\ue9s comme ni\ue9b\ue9 \ue0 double usage pour la zone de for\ueat humide

    TEMPORAL AND SPATIAL DYNAMICS OF Bemisia tabaci POPULATIONS AND CASSAVA VIRAL DISEASES ON SELECTED WHITEFLY RESISTANT CASSAVA GENOTYPES IN UGANDA

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    Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a pest that causes widespread damage on cassava ( Manihot esculanta Crantz), a staple food crop for millions of households in sub-Saharan Africa. Bemisia tabaci also acts as a vector responsible for spreading plant viruses that cause two of the most economically damaging diseases in cassava; namely cassava mosaic disease (CMD) and cassava brown streak disease (CBSD). The objective of this study was to determine the population dynamics of B. tabaci on whitefly resistant cassava genotypes in different agro-ecologies in Uganda. Data were collected once a month, for six months on adult whitefly populations, cassava mosaic disease (CMD) and cassava brown streak disease (CBSD) incidence and symptom severity. The results obtained indicated that cropping season (2015 and 2016), agro-ecology, cassava age and genotypes strongly (P 640.001) influenced the population of adult whitefly. Moderate (47.44% \ub1 0.7821) and high (72.04% \ub1 0.6916) overall means incidences of CBSD recorded across agro-ecologies on the whitefly resistant genotypes in 2015 and 2016 trials, respectively, were likely due to use of cutting-infected planting materials that were not detected through visual inspection at trial establishment. The analysis further revealed that the interaction effect of genotype, agro-ecology and crop age (months after planting: MAP) had a highly significant influence (P 64 0.001) on whitefly abundance and high significant effect (P 640.05) on CMD incidence and severity. The CMD and CBSD incidence as well as symptom severity increased with crop age from 3 months after planting across agro-ecologies. The study demonstrates that whitefly population dynamics and viral disease incidence are influenced by cassava genotypes, agro-ecological differences and crop age (MAP). Information generated could be used to guide the development of appropriate area-specific control strategies to mitigate the effect of whitefly and whitefly-transmitted viral diseases in cassava.Bemisia tabaci (Gennadius) (Hemiptera : Aleyrodidae) est un ravageur qui cause des d\ue9g\ue2ts \ue9tendus sur le manioc ( Manihot esculanta Crantz), une culture vivri\ue8re de base dans des millions de m\ue9nages en Afrique subsaharienne. Bemisia tabaci agit \ue9galement en tant que vecteur responsable de la propagation de virus v\ue9g\ue9taux qui causent deux des maladies les plus dommageables sur le plan \ue9conomique du manioc; \ue0 savoir la maladie de la mosa\uefque du manioc (CMD) et la maladie des stries brunes du manioc (CBSD). L\u2019objectif de cette \ue9tude \ue9tait de d\ue9terminer la dynamique des populations de B. tabaci sur les g\ue9notypes de manioc r\ue9sistants aux aleurodes dans diff\ue9rentes agro-\ue9cologies en Ouganda. Des donn\ue9es ont \ue9t\ue9 recueillies une fois par mois pendant six mois sur les populations d\u2019aleurodes adultes, l\u2019incidence de la maladie de la mosa\uefque du manioc (CMD) et de la maladie des stries brunes du manioc (CBSD) et la gravit\ue9 des sympt\uf4mes. Les r\ue9sultats obtenus ont indiqu\ue9 que la saison de culture (2015 et 2016), l\u2019agro-\ue9cologie, l\u2019\ue2ge et les g\ue9notypes du manioc ont fortement influenc\ue9 (P 640,001) la population d\u2019aleurodes adultes. Les incidences moyennes globales mod\ue9r\ue9es (47,44\ua0% \ub1 0,7821) et \ue9lev\ue9es (72,04\ua0% \ub1 0,6916) de CBSD enregistr\ue9es dans les agro-\ue9cologies sur les g\ue9notypes r\ue9sistants aux aleurodes dans les essais de 2015 et 2016, respectivement, \ue9taient probablement dues \ue0 l\u2019utilisation de mat\ue9riel de plantation infect\ue9 par des boutures qui n\u2019ont pas \ue9t\ue9 d\ue9tect\ue9s par inspection visuelle dans l\u2019\ue9tablissement d\u2019essai. L\u2019analyse a en outre r\ue9v\ue9l\ue9 que l\u2019effet d\u2019interaction du g\ue9notype, de l\u2019agro-\ue9cologie et de l\u2019\ue2ge de la culture (mois apr\ue8s la plantation: MAP) avait une influence hautement significative (P 640,001) sur l\u2019abondance des aleurodes et un effet significatif \ue9lev\ue9 (P 640,05) sur l\u2019incidence et gravit\ue9. L\u2019incidence de CMD et de CBSD ainsi que la s\ue9v\ue9rit\ue9 des sympt\uf4mes ont augment\ue9 avec l\u2019\ue2ge de la culture \ue0 partir de 3 mois apr\ue8s la plantation dans toutes les agro-\ue9cologies. L\u2019\ue9tude d\ue9montre que la dynamique des populations d\u2019aleurodes et l\u2019incidence des maladies virales sont influenc\ue9es par les g\ue9notypes de manioc, les diff\ue9rences agro-\ue9cologiques et l\u2019\ue2ge des cultures (MAP). Les informations g\ue9n\ue9r\ue9es pourraient \ueatre utilis\ue9es pour orienter l\u2019\ue9laboration de strat\ue9gies de contr\uf4le appropri\ue9es et sp\ue9cifiques \ue0 la zone pour att\ue9nuer l\u2019effet des aleurodes et des maladies virales transmises par les aleurodes sur le manioc

    SEED SYSTEM AND SEED QUALITY OF KERSTING\u2019S GROUNDNUT IN BENIN

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    Swift delivery of quality seeds to farming communities is a fundamental step in mainstreaming the value chain of orphan crops. The objective of this study was to characterise seed production and delivery systems of the orphan Kersting\u2019s groundnut (KG) [ Macrotyloma geocarpum (Harms) Mar\ue9chal & Baudet] and their influence on seed quality in Benin. A survey was conducted among 305 farmers from the major KG growing areas in Benin. A total of 60 seed samples were collected from different sources (farmer own seed, local markets and agro-dealers) for quality tests. The results showed that seed sources, acquisition modes and perceived quality differed significantly (P<0.01) among gender groups and with farming experience. Majority of farmers (74.93%) perceived seeds acquired from the local markets and seed stored in non-hermetic conditions as of poor quality compared to seeds sourced from their own stock and stored in hermetic conditions. Yet, the seed quality analysis revealed no differences (P<0.05) among the sources and storage methods. Less than 20% of the tested samples met the national quality standards suggesting an important quality constraint in the current system which may justify the reported low yield and crop failure.L\u2019acc\ue8s aux semences de qualit\ue9 est une \ue9tape fondamentale dans l\u2019int\ue9gration de la cha\ueene de valeur des cultures orphelines. L\u2019objectif de cette \ue9tude \ue9tait de caract\ue9riser les syst\ue8mes de production et de distribution de semences de la lentille de terre [Macrotyloma geocarpum (Harms) Mar\ue9chal & Baudet] au B\ue9nin et leur influence sur la qualit\ue9 des semences. Une enqu\ueate a \ue9t\ue9 men\ue9e aupr\ue8s de 305 agriculteurs de la principale zone de production de la lentille de terre au B\ue9nin. Au total, 60 \ue9chantillons de semences ont \ue9t\ue9 collect\ue9s aupr\ue8s de diff\ue9rentes sources (agriculteurs, march\ue9s locaux et les vendeurs d\u2019intrants agricole) pour des tests de qualit\ue9. Les r\ue9sultats ont montr\ue9 que les sources de semences, les modes d\u2019acquisition et la perception des r\ue9pondants par rapport \ue0 la qualit\ue9 des semences diff\ue9raient significativement (P<0,01) avec le genre des r\ue9pondants et leur l\u2019exp\ue9rience de production. La majorit\ue9 des r\ue9pondants (74,93%) per\ue7oivent les semences acquises sur les march\ue9s locaux et les semences stock\ue9es dans des structures non herm\ue9tiques comme \ue9tant de mauvaise qualit\ue9 par rapport \ue0 celles provenant de leur propre stock et stock\ue9es dans des structures herm\ue9tiques. Cependant, l\u2019analyse de la qualit\ue9 des semences n\u2019a r\ue9v\ue9l\ue9 aucune diff\ue9rence significative (P<0,05) entre les sources et les m\ue9thodes de stockage. Moins de 20 % des \ue9chantillons test\ue9s r\ue9pondaient aux normes de qualit\ue9 en vigueur au Benin, ce qui sugg\ue8re une contrainte de qualit\ue9 importante dans le syst\ue8me actuel et pourrait justifier le faible rendement et l\u2019\ue9chec des r\ue9coltes enregistr\ue9es

    Spatio-temporal Analysis of Urban Growth and Its Effects on Wetlands in Rwanda: The Case of Rwampara Wetland in the City of Kigali

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    This study aimed at analyzing the spatio-temporal patterns of urban growth and its effects on Rwampara wetland, located in the City of Kigali, Rwanda. First, the study was based on the application of remote sensing technology, where 4 Landsat images (1987, 1999, 2009 & 2018) were classified using maximum likelihood classification algorithm. This helped in analyzing the Land Use and Land Cover (LULC) trends in the study area. Secondly, it used the existing LULC data for the years 1990, 2000, 2010 and 2018 in order to investigate the overall changes in LULC in Kigali City. Finally, semi-structured interviews were used to collect data from local people and decision-makers about their past and future management strategies of Rwampara wetland. In this regard, 30 local communities (mainly natives from the study area), 15 local government authorities at sector and district levels as well as 4 senior government authorities in the central administration were interviewed. The findings revealed that over the past 4 decades, urban growth in Kigali City has rapidly increased at the expense of resource degradation in Rwampara wetland. Specifically, there has been an increase of about 77% of the built-up area over the last 31 years (1987-2018) which has led to the decrease of the wetland surface area from 24 ha in 1987 to only 7.7 ha in 2018. The results also revealed that demographic factors (i.e. a high population growth rate and high population densities) were mainly responsible for urban growth and degradation of wetland resources in the area under investigation

    Rural Households\u2019 Attitude towards Potential Use of Jatropha for Environmental Restoration in Saki West Local Government Area of Oyo State, Nigeria

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    The study examined rural households\u2019 attitude towards potential use of Jatropha for environmental restoration in Saki-West Local Government Area. A purposive sampling procedure was used in selecting due to deforestation for charcoal production, while 120 respondents were sampled with a simple random technique from the study area. The data were collected through a well-structured questionnaire with interview section, and were analyzed with frequencies, percentages, mean scores, and Pearson product moment correlation. There was correlation between rural households\u2019 awareness on use of Jatropha and use of Jatropha for environmental restoration (r = 0.399**, p < 0.05). The respondents\u2019 attitude was significantly related to their behavior in utilization of Jatropha for environmental restoration (r = - 0.182*, p < 0.05). The study showed that awareness and attitude of rural households strongly influenced use of Jatropha for environmental restoration. The implication of this study is to ensure that the environment becomes free of hazards and degradation which affect survival of the ecosystem and biodiversity. Hence, government should join in the advocacy to open continuous channels of communication on the importance of Jatropha, and to also motivate the people to having right attitude towards the use of Jatropha as a measure to restoring the environment

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    Bioline International
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