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    Alteration in morpho-physiological attributes of Leymus chinensis (Trin.) Tzvelev by exogenous application of brassinolide under varying levels of drought stress

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    Climate change and prolong drought adversely affect the grassland ecosystem, is precarious environmental constraint restricting plant growth and productivity. An experiment was conducted to alleviate adverse impacts of drought on physiochemical and morphological attributes of Leymus chinensis (Trin.) Tzvelev using brassinosteroid (BR). Treatments comprised well watered control (soil water contents 80%-85%) including Control (T0) and Control+ BR (TB0), moderate drought stress (soil water contents 50%-55%) including (T1) and T1+ BR (TB1) and severe drought stress (soil water contents 30%-35%) including (T2) and T2+ BR (TB2), they were laid out in completely randomized design with five replicates. Drought stress significantly impaired growth, osmotic substances, photosynthetic rate, and other physiochemical process. Moreover, perturbation in recorded attributes was aggravated by increasing drought severity from moderate to severe. Nevertheless, BR application (0.1 mg L-1) improved plant weight (38.8%-46.2%), relative water content (23.4%-29.1%), chlorophyll a (16.6%-56.5%), chlorophyll b (8.5%-16.9%), carotenoids (29.1%-67.3%), soluble sugars (1.7%-29.4%), free amino acids (15.4%-19.4%), and proline content (45.2%-79.4%); while, lowered malondialdehyde (34.8%-45.4%) at each level of drought. Likewise, BR application enhanced net photosynthetic rate (69.8%-165.1%), transpiration rate (45.0%-146.9%), water utilization efficiency (9.3%-16.0%), carboxylation utilization efficiency (80%-250%), sunlight utilization efficiency (66.6%-158.8%) and improved chlorophyll fluorescence characteristics at each level of drought, as compared to untreated controls. The adverse implications on physiochemical and morphological attributes were aggravated with the enhancing severity of drought from control to severe drought. Yet, 0.1 mg L-1 exogenous BR improved morphological and physiochemical attributes remarkably over respective controls and hence can be used to improve growth of drought stressed L. chinensis

    Differences in metabolism of three Conyza species to herbicides glyphosate and triclopyr revealed by LC-MS/MS

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    Conyza spp. are among the most common weeds found in perennial crops, and resistance to several herbicides has already occurred worldwide. In the present study, pot and laboratory experiments were conducted to evaluate differences in glyphosate and triclopyr susceptibility between different biotypes of Conyza spp. Plant material was obtained from seeds collected in perennial crops in Greece. Seeds of four Conyza spp. biotypes were sown in separate pots and treated with glyphosate at the rosette stage, while seeds of six different Conyza spp. biotypes were sown and treated with triclopyr. Twenty-four hours after the herbicide treatment, the aboveground part of the weeds was manually collected and plant samples were prepared to determine the analytes with LC-MS/MS. In two C. bonariensis biotypes, the glyphosate concentration was 50% to 52% lower than for other biotypes; therefore, they could be considered as potentially resistant to glyphosate because the resistant biotype had a stronger and faster metabolism than the sensitive biotype, transforming glyphosate into non-toxic metabolites. The higher glyphosate concentration was detected in C. canadensis biotypes, which proved to be potentially susceptible to glyphosate. Three C. bonariensis biotypes and one C. albida biotype were less susceptible to triclopyr because analytes were detected in 63% to 82% higher concentrations than the two C. canadensis biotypesthat were susceptible to triclopyr. These results reveal significant differences between the three species, confirm the problem of herbicide resistance, and suggest that glyphosate and triclopyr efficacy in Conyza spp. is merely due to herbicide metabolism to other compounds

    Endophytic fungi associated with four endemic wild coffee species (Mascarocoffea) in Madagascar

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    Mascarocoffea are wild coffee plants endemic to Madagascar. These plants produce diverse, often specific biomolecules that are not found in cultivated coffee plants. Production of these compounds could be due to interactions between the endophytes and the host plant. Few studies have been carried out on the richness and diversity of microorganisms associated with these coffee plants. The objective of this study was to identify endophytic fungi isolated from the leaves of species of Mascarocoffea by morphological and molecular methods. Fifteen taxa were morphologically identified among the 30 isolated. These included Phyllosticta sp., Colletotrichum sp., Daldinia sp., Diaporthe sp., Cladosporium sp., Fusarium sp.01, Fusarium sp. 02, Fusarium sp. 03, Monilinia sp., Trichoderma sp., Alternaria sp, Penicillium sp., Aspergillus niger , Rhizopus sp. and Nigrospora sp. The phylum Ascomycota was the most represented, with 14 taxa and 1 taxon (Rhizopus sp.) belonging to the phylum of Zygomycota. Molecular characterisation confirmed of the identity of these 15 taxa and those of the morphologically Unidentified (NI) mycotaxa including Colletotrichum karstii , Colletotrichum siamense , Neofusicoccum parvum , Colletotrichum siamense, Punctularia strigosozonata , Stemphylium solani , Phoma multirostrata , Calophoma complonata, Daldinia vanderguchtiae , Phoma exigua and Boremia exigua . This study allowed us to identify the endophytic fungi isolated from Mascarocoffea leaves from Madagascar.Les Mascarocoffea sont des caf\ue9iers sauvages end\ue9miques de Madagascar. Ces plantes produisent des biomol\ue9cules diversifi\ue9es, souvent sp\ue9cifiques et inexistantes chez les caf\ue9iers cultiv\ue9s. La production de ces compos\ue9s pourrait \ueatre due aux interactions des microorganismes endophytes et la plante h\uf4te. Peu d\u2019\ue9tude a \ue9t\ue9 r\ue9alis\ue9e sur la richesse et la diversit\ue9 des microorganismes associ\ue9s \ue0 ces caf\ue9iers. L\u2019objectif de cette \ue9tude est d\u2019identifier les champignons endophytes isol\ue9s \ue0 partir des feuilles de quatre esp\ue8ces de Mascarocoffea par la m\ue9thode morphologique et mol\ue9culaire. Quinze taxons ont pu \ueatre identifi\ue9s morphologiquement parmi les 30 isol\ue9s. Ce sont\ua0: Phyllosticta sp., Colletotrichum sp., Daldinia sp., Diaporthe sp., Cladosporium sp., Fusarium sp.01, Fusarium sp.02, Fusarium sp. 03, Monilinia sp., Trichoderma sp., Alternaria sp., Penicillium sp., Aspergillus niger , Rhizopus sp. et Nigrospora sp. Le phylum des Ascomycota est le mieux repr\ue9sent\ue9 avec 14 taxons et 1 taxon (Rhizopus sp.) appartient au phylum de Zygomycota. La caract\ue9risation mol\ue9culaire a permis de confirmer l\u2019identit\ue9 de ces 15 taxons et celles des mycotaxons morphologiquement Non Identifi\ue9es (NI) dont Colletotrichum karstii , Colletotrichum siamense , Neofusicoccum parvum , Colletotrichum siamense, Punctularia strigosozonata , Stemphylium solani , Phoma multirostrata , Calophoma complonata, Daldinia vanderguchtiae , Phoma exigua et Boremia exigua . Cette \ue9tude nous a permis d\u2019identifier les champignons endophytes des feuilles de Mascarocoffea de Madagascar

    ANALYSIS OF ADAPTATION DIVERSITY TO CLIMATE VARIABILITY AND CHANGE AMONG PASTORAL COMMUNITIES IN NORTH-EASTERN UGANDA

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    Adaptation framing remains one of the major challenges to achieving greater implementation of adaptation initiatives in Sub-Saharan Africa (SSA). Using an integrated analytical framework that frames adaptation indicators into three dimensions; adaptive, absorptive and transformative capacities, we analysed the adaptation diversity in Karamoja sub-region, Uganda. We found a strong perception of the existence of climate variability and change manifested through the occurrence of droughts, floods, hailstorms, late onset and early rainfall onset. Absorptive capacity revealed varied status of asset ownership, custodianships, and access to these assets, presence of informal social safety nets, and social cohesion. Adaptive capacity revealed the presence of a diversity of livelihood sources, livelihood assets and associated income, but its human capital indicator revealed considerably high illiteracy levels among respondents. Meanwhile, transformative capacity revealed existence of network structures, governance and institutions, facilitated access to early warning information on pests, diseases and rainfall onset. Traditional institutions and the justice system played a key role in conflict resolution, mediation and negotiation for kraals establishment, grazing, and watering rights. We conclude that pastoral communities in Karamoja have a high inclination to maintenance of stability while their flexibility and ability to change decreases with the intensity of change pro-rata.Le cadrage de l\u2019adaptation reste l\u2019un des d\ue9fis majeurs pour parvenir \ue0 une plus grande mise en \u153uvre des initiatives d\u2019adaptation en Afrique subsaharienne (ASS). En utilisant un cadre analytique int\ue9gr\ue9 qui encadre les indicateurs d\u2019adaptation en trois dimensions; capacit\ue9s d\u2019adaptation, d\u2019absorption et de transformation, nous avons analys\ue9 la diversit\ue9 de l\u2019adaptation dans la sous-r\ue9gion de Karamoja, en Ouganda. Nous avons trouv\ue9 une forte perception de l\u2019existence de la variabilit\ue9 et des changements climatiques qui se manifestent par la survenue de s\ue9cheresses, d\u2019inondations, de temp\ueates de gr\ueale, d\u2019apparition tardive et pr\ue9coce des pr\ue9cipitations. La capacit\ue9 d\u2019absorption a r\ue9v\ue9l\ue9 des statuts vari\ue9s d\u2018\ua0actifs\ua0de\ua0propri\ue9t\ue9, de la protection de ses actifs et d\u2019acc\ue8s \ue0 ces actifs, la pr\ue9sence des r\ue9seaux de la s\ue9curit\ue9 sociale informels et la coh\ue9sion sociale. La capacit\ue9 d\u2019adaptation a r\ue9v\ue9l\ue9 la pr\ue9sence d\u2019une diversit\ue9 de sources de subsistance, d\u2019actifs de subsistance et de revenus associ\ue9s, mais son indicateur de capital humain a r\ue9v\ue9l\ue9 des niveaux d\u2019analphab\ue9tisme consid\ue9rablement \ue9lev\ue9s parmi les r\ue9pondants. Pendant ce temps, la capacit\ue9 de la transformation a r\ue9v\ue9l\ue9 l\u2019existence de structures de r\ue9seau, de gouvernance et d\u2019institutions, a facilit\ue9 l\u2019acc\ue8s aux informations d\u2019alerte pr\ue9coce sur les ravageurs, les maladies et l\u2019apparition des pluies. Les institutions traditionnelles et le syst\ue8me judiciaire ont jou\ue9 un r\uf4le cl\ue9 dans la r\ue9solution des conflits, la m\ue9diation et la n\ue9gociation pour l\u2019\ue9tablissement des \ue9tables et les droits de p\ue2turage et d\u2019abreuvement. Nous concluons que les communaut\ue9s pastorales du Karamoja ont une forte tendance au maintien de la stabilit\ue9 tandis que leur flexibilit\ue9 et leur capacit\ue9 \ue0 changer diminuent avec l\u2019intensit\ue9 du changement au prorata

    Assessment of Antimicrobial Effect of Alcohol and Aqueous Extracts of Garcinia kola on Staphylococcus aureus , Bacillus cereus , Escherichia coli and Klebsiella pneumonia

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    As a result of the development of resistance of microorganisms to older antimicrobial agents there is need for a search for new agents, which are effective for the treatment of infections. The crude aqueous and alcoholic extr acts of Garcinia kola fruits were assayed against Staphylococcus aureus, Bacillus cereus, Escherichia coli and Klebsiella pneumoniae. The results revealed that the plant extracts possess inhibitory effect against the microorganisms tested. The minimum inhibitory concentration of the plant extracts ranged between 20mg/ml and 45mg/ml. There was a change in the antibacterial activity of the test extracts on variation of temperature. The results obtained may suggest that the plant extract is thermal stable and could serve as a source of industrial drugs useful in chemotherapy of some microbial infections

    Food Waste Bioeconomy: Sustainable Waste Management Options for Hawassa University Campuses, Ethiopia

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    Food waste management is a challenge in University Campuses of developing countries. This study assessed food waste management challenges in Hawassa University and the possibility of cascading the waste through biomass bioeconomy model by using interviews, observations and published and unpublished documents. The results show that so far the food leftover is being used by poor people, collected by animal ranchers or damped in an open-pit. Food leftover use by poor people was challenged due to poor hygienic quality, health implication to users, insecurity to campus community and theft of property in the campuses. The university\u2019s animal enterprise was also forced to quiet its agreement with the university due people\u2019s competition for the leftover. Generally food waste management at the University is reactive and long-term sustainability is needed. This study suggests the cascading use of biomass, i.e. using food waste as animal feed; animal waste as feedstock for biogas generation; biogas-slurry as an organic fertilizer for university farm and plantations. If implemented the model improves the waste management practices of the University; improves the resource use efficiency and energy security, and reduces fuel wood consumption and mitigate greenhouse gas emission. Moreover the model creates circular economy that serves as a sustainability showcase in practice for research, training, recreation, experience sharing and income generation activities

    Fibre Characterization of Cassava Peel Leaf Meal and Its Utilization by Broilers

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    One hundred and twenty ( n = 120) day old broiler chicks with an average weight (100.00\ub17.00g) were randomly allotted to four dietary treatments, with cassava peel leaf meal (CPLM) as replacement for maize at (starter and finisher phases).The trial was in three phases of adaptation (1-2week),starter (3-5week) and finisher (6-9week).Thirty broilers chicks were assigned to each of the four treatments, while was replicated three times at 10 chicks each. Performance of the chicks such weight gain, feed intake and carcass yield was evaluated and the proximate and fiber quality of the diets and CPLM were also determined. Data obtained for performance evaluated was analyzed using ANOVA, while significant means were separated by SAS of the same package. The outcome revealed that CPLM had (89.93%) dry matter, (18.93%) crude protein and (43.28%) NDF, with anon-significant (p< 0.05) variation in weight gain, enhanced (p< 0.05) feed intake and depressed (p< 0.05) feed to gain ratio as CPLM inclusion increased. Dressing percentage values (69.00-73.00%) were obtained on the treatments. CPLM inclusion (0, 15, 30 and 45%) as replacement for maize in the diets of broilers produced a favourable performance

    Socio-Economic Importance of Honey among Traditional Healers and Orthodox Practitioners in Iwo Local Government Area of Osun State, Nigeria

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    The necessity for people\u2019s adequate and quality as well as good health and standard of living for overall National development informed the main reason for the study on Socio-economic importance of honey among traditional healers and orthodox medical practitioners in Iwo Local Government Area, Osun State. A total of 105questionnaires were administered, while 91 were retrieved from the respondents. Various uses of honey were investigated both for personal consumption and medicinal purpose. The result shows that 41.8 % of the respondent use honey for multiple purpose, while 35.2% use honey mainly for medicinal purpose, 16.5% use it mainly as beverages, while the remaining 6.6% for use in treatments. The Chi Square Test showed a higher calculated value of 385.71 than the tabulated value of 21.026. This confirms a strong relationship between the use of honey and social life of Iwo people. The major constraints discovered was that Traditional healers do not exercise strict control on the quantity doses of honey used for different ailments. It is therefore recommended that both rural and urban dwellers should be sensitized about the use of honey, in order to encourage sustainable consumption for improvement of health status

    Market Opportunities and Profitability of Tree Crop Planting in Nigeria

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    Investing in trees offer financial and social returns for years to come and it is a lucrative long-term investment in Nigeria. Despite the substantial gain on investment in tree planting, only few people are still involved in the business. This paper analyzed the profitability of investment in tree crop planting for income generation. Benefit Cost Ratio (BCR) and Net Present Value (NPV) were calculated to determine the financial viability of tree crop planting. The study showed that tree crop planting is a profitable business as shown by positive NPV of \u20a62,187,822.0 and \u20a62,782,214.97 for Tectonia grandis and Moringa oleifera plantations respectively, after 10-years of maturation and harvesting at 14 % discount rate with a B/C Ratio of 1.97 and 1.6 respectively. Also, NPV of \u20a61, 575,487.10 for a 5-year small scale private nursery establishment with a B/C Ratio of 2.79 at a 14% discount rate also confirm that the investment on nursery establishment is highly profitable. It is recommended that with adequate support and incentives, farmers can take advantage of these opportunities and invest in nursery enterprise and tree crop sub-sector of the economy

    Grain Size and Heavy Minerals Analysis of Maastrichtian Sandstone, Anambra Basin, Nigeria: Implication for Aquifer Properties

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    The Maastrichtian sediments of Anambra Basin, south western Nigeria was studied for grain characteristics and maturity of the sediments, so that by combining sedimentological characteristics, maturity and its paleoenvironment of deposition of the Ajali sandstone, in order to ascertain the aquifer properties of the groundwater within the study area and by extension the availability of drinking water for both the urban and rural settlers. Grain size analysis (51 samples) and heavy mineral analysis (15 samples each) were analyzed. The textural parameters show that the Sandstone are medium sand, poorly to moderately sorted, coarse to strongly coarse skewed with mesokurtic to leptokurtic grains. The heavy minerals present in lithologic sand and sandstone units of Ajali Formation are mainly zircon, rutile, tourmaline, apatite, staurolite, and opaque minerals (Goethite, hematite, ilmenite), these heavy minerals found in the sandstone are associated with igneous and metamorphic source which indicates that these maastritchian sediments are from basement complex rock The ZTR index of 67.96% and the values of the kurtosis indicates a sub-mature sandstone to mature sandstone, which gives an excellent aquifer properties. Also, the paleoenvironment of deposition of the Maastrichtian sediments is Fluvial. The sand and sandstone bodies deposited in a fluvial system have sheet-like geometries and due to the differences in the extent of aquifer compartmentalization, Sandstone deposited in fluvial environment normally has better hydraulic conductivity which again reveals a good aquifer property. The sedimentological characteristics for the Sandstone exposed at Fugar and environs, is a coarsening upward sequence (increasing grain sizes) with a progressive increase in sorting. since hydraulic conductivity increases with increasing grain sizes and sorting, hence, excellent aquifer characteristics for the groundwater within the study area. The foregoing revealed that the aquifer geometry and properties of the study areas is favourable for the availability of drinking water for both the Urban and rural dwellers within these communities

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