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Evaluating green suppliers: improving supplier performance with DEA in the presence of incomplete data
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Prosedur Pengelolaan Utang Terhadap Pihak Ketiga Pada PT. PLN (Persero) Sektor Pengendalian Pembangkit Bandar Lampung [ Laporan Akhir - CD ]
No full textPengaruh Konsumsi Kopi Lampung (Coffea Robusta) 100% Terhadap Kadar Low Density Lipoprotein (LDL) Dalam Darah Pada Mahasiswa Fakultas Kedokteran Universitas Lampung [ Skripsi-CD ]
No full textAnalisis potensi ekowisata birdwatching di Pusat Latihan Gajah Taman Nasional Way Kambas [Skripsi]
No full textBib. hal. 62-66xv, 61 hlm. : il. ; 28 cm
Hubei poty?like virus 1 is likely an interspecies recombinantof sugarcane mosaic virus and putative bean yellow mosaic virus
No full textMolecular characterization and analysis of conserved potyviral motifsin bean common mosaic virus (BCMV) for RNAi?mediated protection
No full textAbstractAustralian bean common mosaic virus (BCMV) isolates were sequenced, and the sequences were compared to global BCMVand bean common mosaic necrosis virus (BCMNV) sequences and analysed for conserved potyviral motifs to generate inplanta RNA-interference (RNAi) resistance. Thirty-nine out of 40 previously reported potyvirus motifs were conservedamong all 77 BCMV/BCMNV sequences. Two RNAi target regions were selected for dsRNA construct design, covering920 bp of the nuclease inclusion b (NIb) protein and 461 bp of the coat protein (CP). In silico prediction of the effectivenessof these constructs for broad-spectrum defence against the 77 BCMV and BCMNV sequences was done via analysis ofputative 21-nucleotide (nt) and 22-nt small-interfering RNAs (siRNAs) generated from the target regions. The effectivenessof both constructs for siRNA generation and BCMV RNAi-mediated resistance was validated in Nicotiana benthamianatransient assays
Spatial distribution of orally administered viral fusolin proteinin the insect midgut and possible synergism between fusolinand digestive proteases to disrupt the midgut peritrophic matrix
No full textAbstractOral inoculation of entomopoxvirus spindles, microstructures composed of fusolin protein, causes disruption of the peritrophicmatrix (PM), a physical barrier against microbe infection, in the insect midgut. Although the atomic structure offusolin has been determined, little has been directly elucidated of the mechanism of disruption of the PM. In the presentstudy, we first performed an immunohistochemical examination to determine whether fusolin acts on the PM directly orindirectly in the midgut of Bombyx mori larvae that were inoculated with spindles of Anomala cuprea entomopoxvirus. Thisrevealed that the PM, rather than the midgut cells, was the attachment site for fusolin. Fusolin broadly attached to the PMfrom the anterior to the posterior region, both to its ectoperitrophic and endoperitrophic surfaces and within the PM. Theseresults likely explain why the whole of the PM is rapidly disintegrated. Second, we administered protease inhibitors mixedwith spindles and observed decreased midgut protease activity and reduced disruption of the PM. This suggests that midgutprotease(s) is also positively involved in PM disruption. Based on the present results, we propose an overall mechanism forthe disruption of the PM by administration of fusolin
Vector competence analysis of two Aedes aegypti lineages from Bello,Colombia, reveals that they are affected similarly by dengue?2 virusinfection
No full textAbstractDengue is the second most prevalent vector-borne disease after malaria in Colombia. It is caused by dengue virus, anarbovirus that exhibits high epidemic power, which is evidenced by its occurrence in more than 80% of the country, largelybecause of the extensive dispersion of the mosquito vector Aedes aegypti. The existence of two lineages of Ae. aegypti hasbeen proposed based on genetic differences at the mitochondrial level, and they have been reported to circulate in similarproportions in the municipality of Bello (Colombia). It has been suggested that the differentiation of these lineages couldinfluence features such as vector competence (VC) and life table. With the aim of testing this hypothesis, female mosquitoesfrom both lineages collected from Bello were orally challenged with dengue virus serotype 2 (strain D2-HAN) to measureinfection, dissemination, survival and fecundity. Analysis of VC showed an increase in viral titer over time; however, nosignificant differences were observed between the lineages. The survival rate was not different between the infected lineages,but comparing lineages, it was lower in infected mosquitoes, which may affect the intensity of transmission. Finally,we conclude that the genetic differentiation of Ae. aegypti into lineages did not confer differences in epidemiological statuswhen the mosquitoes were infected with this D2 serotype strain