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Characterization of a novel lytic myophage, phiA8?29, infectingAeromonas strains
No full textAbstractMembers of the bacterial genus Aeromonas are important aquatic pathogens that cause severe fish diseases. Here, we characterizea novel lytic phage, Aeromonas virus phiA8-29, isolated from the alimentary tract of a freshwater fish. Transmissionelectron microscopy showed that phiA8-29 has a long contractile tail and thus can be classified as a member of the familyMyoviridae. The phage genome was identified as a double-stranded DNA molecule of 144,974 bp containing 185 openreading frames and nine tRNA-encoding genes. Comparative genomic analysis revealed that the phiA8-29 genome has littlesimilarity to any of the currently sequenced Aeromonas phage genomes. Our data indicate that phiA8-29 is a novel lyticMyoviridae phage that does not belong to any of the known genera
Classification of hospital admissions into emergency and elective care: a machine learning approach
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Manajemen perawatan tandan buah pisang mas (Musa acuminata) di Kelompok Tani Nakula Dusun Tanjung Sari Pekon Margoyoso Kecamatan Sumberejo Tanggamus [ CD-Tugas Akhir ] /Rizki Pratama
No full textPISANG 634.77 Pra
Strategi penetapan harga pada perusahaan PT.Penerbit Erlangga di Wilayah Bandar-Lampung [ CD-Tugas Akhir ] / Dian Afriyanto
No full textMANAJEMEN PENJUALAN658.81 Afr s c.
Strategi pelayanan untuk meningkatkan loyalitas nasabah pada PT.Pegadaian (PERSERO) UNIT PELAYANAN CABANG Way Dadi Bandar Lampung [ CD-Tugas Akhir ] /Astri Putri Hidayani
No full textPELAYANAN KONSUMEN658 Hid s c.
Manajemen panen pisang mas ( Musa acuminata ) di Kelompok Tani Arjuna Dusun IV Sailing Pekon Sumbermulyo Kecamatan Sumberejo Kabupaten Tanggamus Provinsi Lampung [ CD-Tugas Akhir ] /Hersantori Yusuf
No full textPANEN--PISANG631.55 Yus
Manajemen pengendalian hama dan penyakit pada tanaman karet ( Hevea brasiliensis Muell.Arg ) di PT. Huma Indah Mekar Desa Penumpang Baru Kecamatan Tulang Bawang Tengah Kabupaten Tulang Bawang Barat Provinsi Lampung [ CD-Tugas Akhir ] / Megi Yantori
No full textPENYAKIT TANAMAN KARET632.3 Yan
Complete genome sequence of a novel bacteriophage, PBKP05,infecting Klebsiella pneumoniae
No full textAbstractAn increasing number of Klebsiella pneumoniae isolates have been found to be multi-drug resistant. A novel bacteriophage,PBKP05, which infects K. pneumoniae, was isolated and characterized. It has a linear double-stranded DNA genome of30,240 base pairs in length. Its G+C content is 53%, and 47 putative open reading frames are functionally annotated. Thisphage can be a candidate material for phage therapy
Molecular surveillance of coxsackievirus A16 reveals the emergenceof a new clade in mainland China
No full textAbstractCoxsackievirus A16 (CV-A16) of the genotypes B1a and B1b have co-circulated in mainland China in the past decades.From 2013 to 2017, a total of 3,008 specimens from 3,008 patients with mild hand, foot, and mouth disease were collectedin the present study. Viral RNA was tested for CV-A16 by a real-time RT-PCR method, and complete VP1 sequences andfull-length genome sequences of CV-A16 strains from this study were determined by RT-PCR and sequencing. Sequenceswere analyzed using a series of bioinformatics programs. The detection rate for CV-A16 was 4.1%, 25.9%, 10.6%, 28.1% and12.9% in 2013, 2014, 2015, 2016 and 2017, respectively. Overall, the detection rate for CV-A16 was 16.5% (497/3008) inthis 5-year period in Shenzhen, China. One hundred forty-two (142/155, 91.6%) of the 155 genotype B1 strains in the studybelonged to subgenotype B1b, and 13 (13/155, 8.4%) strains belonged to subgenotype B1a. Two strains (CVA16/Shenzhen174/CHN/2017 and CVA16/Shenzhen189/CHN/2017) could not be assigned to a known genotype. Phylogenetic analysisof these two strains and other Chinese CV-A16 strains indicated that these two CV-A16 strains clustered independently in anovel clade whose members differed by 8.4%-11.8%, 8.4%-12.1%, and 14.6%-14.8% in their nucleotide sequences from thoseof Chinese B1a, B1b, and genotype D strains, respectively. Phylogenetic analysis of global CV-A16 strains further indicatedthat the two novel CV-A16 strains from this study grouped in a previously uncharacterized clade, which was designated asthe subgenogroup B3 in present study. Meanwhile, phylogenetic reconstruction revealed two other new genotypes, B1d andB4, which included a Malaysian strain and two American strains, respectively. The complete genome sequences of the twonovel CV-A16 strains showed the highest nucleotide sequence identity of 92.3% to the Malaysian strain PM-15765-00 from2000. Comparative analysis of amino acid sequences of the two novel CV-A16 strains and their relatives suggested thatvariations in the nonstructural proteins may play an important role in the evolution of modern CV-A16