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Identification of a novel bipartite nuclear localization signalin the DNA polymerase of the betabaculovirus Pieris rapaegranulovirus
No full textAbstractDNA polymerase (DNApol) is highly conserved in all baculoviruses and plays an essential role in viral DNA replication.Previous results showed that the DNApol of the betabaculovirus Pieris rapae granulovirus (PiraGV) can localize in thenucleus. However, it is not clear how the DNApol is transported into the nucleus. Bioinformatic and GFP localization analysisshowed that PiraGV DNApol contains a nuclear localization signal (NLS) at aa 4-25 (LFKRKLDEPPTDHTLVKAIKLS)of the N-terminus that does not match either the classical monopartite or the bipartite NLS consensus sequence. Multiplepoint-substitution analysis confirmed that the NLS is required for transport of PiraGV DNApol into the nucleus. We alsosubstituted the NLS of the PiraGV DNApol for that of the alphabaculovirus Spodoptera litura nuclear polyhedrosis virus(SpltNPV) DNApol. A viral growth curve and quantitative real-time PCR revealed that the substitution impaired viral DNAreplication and resulted in a reduction in virus production. Together, our results show that PiraGV contains a novel NLS andthat the NLS cannot efficiently replace that of SpltNPV DNApol for viral DNA synthesis and virus production
Alteration and Role of Interhemispheric and IntrahemisphericConnectivity in Motor Network After Stroke
No full textAn intelligent truck scheduling and transportation planning optimization model for product portfolio in a cross-dock
No full textConcurrent design of cell formation and scheduling with consideration of duplicate machines and alternative process routings
No full textInventory management for a remanufacture-to-order production with multi-components (parts
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Boundary Spanning Strategy: Case of Hong Kong Firms
No full textHong Kong firms, named as Hong Kong-owned firms in the China mainland, inthe Pearl River Delta contributed to economic development much during the pastmany years. With the global economic structure changing in the recent years,plenty of Hong Kong-owned firms in the China mainland are increasingly facingthe new challenges. This chapter is to explore the boundary spanning for thetransformation strategies, which can be used by the Hong Kong-owned firmsconfronting with the transformation challenges. Geographic boundary spanningdriven transformation strategy, organizational boundary spanning driven transformationstrategy, and technological boundary spanning driven transformationstrategy will be discussed in this chapter. And the conclusion shows that it isnecessary to combine the three transformation strategies according to th
