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Pekerjaan sistem pemipaan air bersih air buangan, Air hujan, Penanggulangan Api Ringan (APAR) pada proyek pembangunan gedung Kantor Dinas Kesehatan Kota Bandar Lampung [CD-Laporan Kerja Praktek] /Nova Tri Astuti
No full text725.5 Ast p c.
Sistem informasi trainer berbasis WEB di PT Great Gian Pineapple [CD-Tugas Akhir] / Septia Wulandari
No full textSISTEM INFORMASI 005.36 Wul
Perancangan dan pembuatan profil Sekolah berbasis WEBSITE di SMK PGRI 2 Bandar Lampung [ CD-Tugas Akhir ] /Tri Wahyuni
No full textWEBSITE 004.678 Wah
Prosedur pemulihan plois atas penunggakan pembayaran premi pada AJB Bumiputera 1912 Cabang TanjungkArang [ CD-Laporan Akhir ] /Irma Lestari Manik
No full textASURANSI JIWA 368.3 Man
Sitem akuntansi pembelian bahan baku pada PT Lambang Jaya [ CD-Laporan Akhir ] /Melinda Saputri
No full textAKUNTANSI 657 Sap
Sistem akuntansi penggajian Penerima Penghasilan Non Pegawai Negeri (PPNPN) pada KPP Pratama Tanjung Karang [ CD-Laporan Akhir ] / Wulan Puspita Sari
No full textGAJI 658.32 Sar
No full textAbstractIn the winter of 2017-2018, there was significant influenza activity in China, resulting in unprecedented usage of influenzarapid antigen tests (IRAT) and neuraminidase inhibitors (NAIs). The aim of this study was to characterize the most prevalentinfluenza virus type in a clinical setting with respect to diagnosis and concomitant NAI treatment. From Dec 2017 to Jan 2018,3257 patients with influenza-like illness (ILI) were screened using IRAT. We summarized and compared the results with thelast influenza season. Subtyping of influenza B viruses and identification of NAI drug resistance mutations were carried outby sequencing the HA and NA genes and aligning these with genetic isotypes. The performance of IRAT and RT-PCR wascompared. Screening results indicated that influenza B virus was the leading cause of this influenza epidemic, with childrenbeing more susceptible to infection than adults. Phylogenetic analysis revealed that the prevailing influenza B virus belongedto the Yamagata lineage and were genetically similar to strains isolated from North America in the same influenza season.Cross-continental spread of influenza/B/Yamagata occurred. NAI resistance mutations were not identified in the 18 samplesanalyzed. The current antiviral protocol was still effective for influenza B control. RT-PCR positivity was significantly higherthan that of IRAT (P = 0.004). IRAT and RT-PCR had a consistency rate of 86.9%, with the consistency rates of the positiveand negative cases being 54.3% and 97.3%, respectively. Clinicians should be alert to the possibility of obtaining falsenegative results when using IRAT, and RT-PCR is recommended to improve the accuracy of pathogen detection
Multiplex one?step real?time PCR assay for rapid simultaneousdetection of velogenic and mesogenic Newcastle disease virusand H5?subtype avian influenza virus
No full textAbstractH5 avian influenza virus (AIV) and velogenic Newcastle disease virus (v-NDV) are pathogens listed in the OIE TerrestrialAnimal Health Code and are considered key pathogens to be eliminated in poultry production. Molecular techniques forrapid detection of H5 AIV and v-NDV are required to investigate their transmission characteristics and to guide prevention.Traditional virus isolation, using embryonated chicken eggs, is time-consuming and cannot be used as a rapid diagnostictechnology. In this study, a multiplex real-time RT-PCR (RRT-PCR) detection method for six H5 AIV clades, three v-NDVsubtypes, and one mesogenic NDV subtype was successfully established. The detection limit of our multiplex NDV and H5AIV RRT-PCR was five copies per reaction for each pathogen, with good linearity and efficiency (y = ?3.194x + 38.427for H5 AIV and y = ?3.32x + 38.042 for NDV). Multiplex PCR showed good intra- and inter-assay reproducibility, withcoefficient of variance (CV) less than 1%. Furthermore, using the RRT-PCR method, H5 AIV and NDV detection rates inclinical samples were higher overall than those obtained using the traditional virus isolation method. Therefore, our methodprovides a promising technique for surveillance of various H5 AIV clades and multiple velogenic and mesogenic NDVsubtypes in live-poultry markets