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The effects of ownership identity on corporate diversification strategy of Chinese companies in foreign markets
No full textIndependent evolution of porcine reproductive and respiratorysyndrome virus 2 with genetic heterogeneity in antigenic regionsof structural proteins in Korea
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Characterization of newly emerged NADC30?like strains of porcinereproductive and respiratory syndrome virus in China
No full textAbstractDifferent strains of porcine reproductive and respiratory syndrome virus (PRRSV) have emerged and circulated in differentregions of mainland China since 1996, particularly after 2006. In 2012, NADC30-like PRRSV was first isolated in HenanProvince. By 2016, it had spread to most provinces in China. In the present study, the whole genomes (excluding the poly(A)tails) of 13 newly emerged NADC30-like PRRSV strains were sequenced and analyzed. Furthermore, the pathogenicity ofSD53-1603, one of the 13 PRRSV strains, was assessed. Phylogenetic analysis showed that these 13 newly emerged NADC30-like PRRSV strains, together with some reference strains, formed a new subgroup (subgroup 5), characterized by a predicted131-amino-acid deletion in the nonstructural protein (NSP) 2. However, low levels of whole-genome similarity and a widevariety of recombination patterns complicated the classification of the NADC30-like PRRSV isolates. Interestingly, almostall of the recombination breakpoints found in these 13 PRRSV isolates and other NADC30-like PRRSV isolates occurred ingenes encoding NSPs and/or minor structural proteins. In addition, piglets infected with the newly emerged NADC30-likestrain SD53-1603 displayed clear clinical respiratory symptoms and underwent typical pathological changes. The findingsmay be useful for elucidating the characteristics and epidemic status of NADC30-like PRRSV in China
Development and application of high?resolution melting analysisfor the classification of infectious laryngotracheitis virus strainsand detection of recombinant progeny
No full textAbstractLive attenuated vaccines against infectious laryngotracheitis virus (ILTV) are widely used in the poultry industry to controldisease and help prevent economic losses. Molecular epidemiological studies of currently circulating strains of ILTV withinpoultry flocks in Australia have demonstrated the presence of highly virulent viruses generated by genomic recombinationevents between vaccine strains. In this study, high-resolution melting (HRM) analysis was used to develop a tool to classifyILTV isolates and to investigate ILTV recombination. The assay was applied to plaque-purified progeny viruses generatedafter co-infection of chicken embryo kidney (CEK) monolayers with the A20 and Serva ILT vaccine strains and also toviruses isolated from field samples. The results showed that the HRM analysis is a suitable tool for the classification ofILTV isolates and can be used to detect recombination between ILTV vaccine strains in vitro. This method can be used toclassify a broad range of ILTV strains to facilitate the classification and genotyping of ILTV and help to further understandrecombination in these viruses
Adecisionsupportsystemforreal-timeschedulingofmultiplepatient classesinoutpatientservices
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Factors affecting platform default risk in online peer-to-peer (P2P) lending business: an empirical study using Chinese online P2P platform data
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