30732 research outputs found
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Peranan lingkungan Fisika dalam memasarkan produk Mahasiswa Center for Career and Entrepreneurship Development (CCED) Storedi UPT Pengembangan Karir dan Kewirausahaan Universitas Lampung [ CD-Laporan Akhir] / Muhammad Agung Hari P.
No full textMANAJEMEN PENJUALAN658.82 Har p c.
Manajemen panen kelapa sawit ( Elaeis guineensis Jacq.) di PT.Bumi Madu mandiri Blambangan Umpu Kabupaten Way Kanan [ CD -Tugas Akhir ] / Riski Kurniawan
No full textPANEN--KELAPA SAWIT631.55 Kur mc.
Manajemen panen tanaman karet ( Hevea brasilliensis ) di PTPN VII Unit Way Berulu Kecamatan Gedung Tataan Kabupaten Pesawaran [ CD-Tugas Akhir ] / Candra Septiawan
No full textPANEN--KELAPA SAWIT631.55 Sep
Manajemen pemeliharaan tanaman belum menghasilkan (TBM) karet ( Hevea brasiensis Muell. ) di PT Perkebunan Nusantara VII Unit Way Berulu [ CD-Tugas Akhir ] / Arpan Sahrul
No full textPOHON KARET--PENANAMAN633.8 Sah
Lipid; Biokimia, Pencernaan, Penyerapan dan Transpornya di Dalam Tubuh
No full textv, 62 hlm, : il. ; 20 x 28,5 cm
Chilo iridescent virus encodes two functional metalloproteases
No full textAbstractThe genome of Chilo iridescent virus (CIV) has two open reading frames (ORFs) with matrix metalloprotease (MMP)domains. The protein encoded by ORF 136R contains 178 amino acids with over 40% amino acid sequence identity tohypothetical metalloproteases of other viruses, and the protein 165R contains 264 amino acids with over 40% amino acidsequence identity to metalloproteases of a large group of organisms, primarily including a variety of Drosophila species.These proteins possess conserved zinc-binding motifs in their catalytic domains. In this study, we focused on the functionalanalysis of these ORFs. They were cloned into the Autographa californica multiple nucleopolyhedrovirus (AcMNPV)Bac-to-Bac baculovirus expression-vector system, expressed in insect Sf9 cells with an N-terminal His tag, and purifiedto homogeneity at 72 hours postinfection using Ni-NTA affinity chromatography. Western blot analyses of purified 136Rand 165R proteins with histidine tags resulted in 24- and 34-kDa protein bands, respectively. Biochemical assays with thepurified proteins, performed using azocoll and azocasein as substrates, showed that both proteins have protease activity. Theenzymatic activities were inhibited by the metalloprotease inhibitor EDTA. Effects of these proteins were also investigated onGalleria mellonella larvae. Insecticidal activity was tested by injecting the larvae with the virus derived from the AcMNPVbacmid carrying 136R or 165R ORFs. The results showed that the baculoviruses harbouring the iridoviral metalloproteasescaused early death of the larvae compared to control group. These data suggest that the CIV 136R and 165R ORFs encodefunctional metalloproteases. This study expands our knowledge about iridoviruses, describes the characterization of CIVmatrix metalloproteinases, and might ultimately contribute to the use of this virus as a research tool
Complete genome sequence of an alternavirusfrom the phytopathogenic fungus Fusarium incarnatum
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Expression, purification and virucidal activity of two recombinantisoforms of phospholipase A2from Crotalus durissus terrificus venom
No full textAbstractThe global emergence and re-emergence of arthropod-borne viruses (arboviruses) over the past four decades have becomea public health crisis of international concern, especially in tropical and subtropical countries. A limited number of vaccinesagainst arboviruses are available for use in humans; therefore, there is an urgent need to develop antiviral compounds.Snake venoms are rich sources of bioactive compounds with potential for antiviral prospection. The major component ofCrotalus durissus terrificus venom is a heterodimeric complex called crotoxin, which is constituted by an inactive peptide(crotapotin) and a phospholipase A2(PLA2-CB). We showed previously the antiviral effect of PLA2-CB against denguevirus, yellow fever virus and other enveloped viruses. The aims of this study were to express two PLA2-CB isoforms in aprokaryotic system and to evaluate their virucidal effects. The sequences encoding the PLA2-CB isoforms were optimizedand cloned into a plasmid vector (pG21a) for recombinant protein expression. The recombinant proteins were expressedin the E. coli BL21(DE3) strain as insoluble inclusion bodies; therefore, the purification was performed under denaturingconditions, using urea for protein solubilization. The solubilized proteins were applied to a nickel affinity chromatographymatrix for binding. The immobilized recombinant proteins were subjected to an innovative protein refolding step, whichconsisted of the application of a decreasing linear gradient of urea and dithiothreitol (DTT) concentrations in combinationwith the detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate (CHAPS) as a protein stabilizer.The refolded recombinant proteins showed phospholipase activity and virucidal effects against chikungunya virus, denguevirus, yellow fever virus and Zika virus