Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences
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MICROSTRUCTURAL CHARACTERISTICS OF THE Longissimus dorsi MUSCLE IN BRAHMAN CROSS CATTLE
Full text linkThis study aimed to analyze the muscle microstructure of longissimus dorsi Brahman cross (BX) cattle slaughtered at the slaughterhouse of North Aceh District. The meat samples for the study were taken from three male BX cattle aged 18-30 months with body weights ranging from 400 kg to 500 kg and the average body condition scores (BCS) of 3-4. The muscle used was the longissimus dorsi muscle. The mean (SE) diameter and cross-sectional area (CSA) of muscle fibers and the percentage of fast fibers in the longissimus dorsi BX muscle were 71.691.23 m, 4263.43305.69 m2 , and 48.178.81%, respectively. This study concluded that the longissimus dorsi muscle of the BX has relatively large muscle fibers, some of which in each fascicle are composed of fast muscle (fast fiber/type II)
CARCASS YIELDS AND ORGANS OF BROILERS FED THE DIET CONTAINING THE FERMENTED MORINGA LEAF MEAL + YELLOW CORN
Full text linkThe purpose of this study was to evaluate the characteristics of carcass yields and internal and external organs of the broilers fed the diets containing the fermented moringa leaf meal (FMOL) + yellow corn (YC), the moringa-corn (MC) diet formulated to replace parts of the commercial diet (CD). As many as 100 DOC of broiler chicken strain CP 707 were reared for up to 5 weeks to feed experimental diets. A Block Randomized Design (BRD) with subsamples consisted of 4 treatments, 5 blocks, and 2 subsamples were applied in the recent study. The results of studies indicated that removing parts of the CD diet to substitute with the mixture of FMOL + YC caused a significant effect (P0.05) in declining the weights of the whole carcass and the cut-ups but in percentage no significant differences. The weights and percentages of all examined organs were not significantly affected (P0.05). It was concluded that using the moringa mixed with yellow corn reduced the weight of the whole carcass and cut-up carcasses but it could use up to 7.5% in the diet without detrimental effects on the internal and external organs of the broilers
CHARACTERIZATION OF GLYCOCUNJUGATE DISTRIBUTION IN THE EPIDIDYMIS OF THE JAVAN MUNTJACS (Muntiacus muntjak muntjak)
Full text linkThe presence of glycoconjugates in caput, corpus, and cauda epididymis of the Javan muntjacs was performed using lectin histochemical method. The epididymis organs were obtained from two adult male Javan muntjacs in hard antler stage and then immediately fixed in Bouins solution and processed in histological manner. Six types of biotinylated lectins namely Peanut agglutinin (PNA), Soybean agglutinin (SBA), Wheat germ agglutinin (WGA), Ricinus communis agglutinin (RCA), Concanavalin A (Con A), and Ulex europaeus agglutinin I (UEA I) were applied to detect the specific glycoconjugates in the epididymal tissue of muntjacs. The result showed the presence of glycoconjugates were detected by five types of lectins except UEA I in caput, corpus, and cauda epididymis with specific distribution and also the intensity of lectins binding. Glycoconjugates -galactose, -glucose, mannose, N-acetyl-galactosamine, N-acetyl-glucosamine, and sialic acid were stained with various intensity in epithelial cells, basal cells, stereocilia, spermatozoa, and stroma of caput, corpus, and cauda epididymis. The presence of sialic acid on the surface of spermatozoa detected by WGA are thought to be play a role on sperm protection during their maturation and transit along epididymal lumen. In conclusion, the difference of glycoconjugates distribution pattern and lectin binding in the epididymal caput, corpus, and cauda of the Javan muntjac indicates the regional differences in epididymal function in spermatozoa maturation
THE INFLUENCE OF NUTRITIONAL FLUSHING ON BODY MORPHOMETRICS OF FEMALE BREEDING GOATS
Full text linkThe purpose of this current study was to evaluate the influence of nutritional flushing on body morphometrics of female breeding goats. This study used 32 local female goats or does with an average age of 3.281.08 years and an average body weight of 42.478.28 kg. The goats were distributed in a completely randomized design using a 2 x 2 factorial pattern with 8 replications. The first factor was the flushing feed (FF) type, consisting of rice bran and concentrate, whereas the second factor was the level of tannin-protected arginine (TPA: 0 and 300 mg/kg). The body morphometrics observed in this study were initial body length (IBL), final body length (FBL), body length change (BLC), initial chest girth (ICG), final chest girth (FCG), chest girth change (CGC), initial wither height (IWH), final wither height (FWH), and wither height change (WHC). The IBL, FBL, BLC, ICG, FCG, IWH, FWH, and WHC of the local does were not significantly different (P0.05) after receiving the FF type. However, FF significantly affected the CGC of the goats (P0.001). The use of concentrate for the local female local goats resulted in a higher CGC (P0.001) compared to the use of rice bran. On the other hand, TPA did not significantly affect all body morphometrics of the goats (P0.05). Thus, it can be concluded that the use of concentrate leads to higher chest girth change, giving better body growth compared to the use of rice bran. The addition of tannin-protected arginine was also found to have no significant contribution to the body morphometrics of the local female goats. ____________
A CLOSE POSITIVE ASSOCIATION BETWEEN OBESITY AND BLOOD PRESSURE IN RATS
Full text linkThe purpose of this study was to analyze how the effect of each increase in Body Mass Index (BMI) on the increase in blood pressure (BP). The research design was a laboratory experiment with 52 non-obese and obese rats as subjects (non-obese, n=14 and obese, n=38). Rats were induced obesity using a high-fat diet for four weeks. BMI used is based on the Lee Index with obesity indicators if 300 g/cm. Data analysis using independent sample t-test, simple linear regression test, and Pearson correlation test; (p0.05). The results: BP in non-obese rats was lower than obese rats (89.007.38 vs 190.114.42; p=0.001*), BMI was positively associated with BP (p=0.001*); an increase in BMI will increase BP (p=0.001*), there is a strong correlation between BMI and BP (r=0.977; p=0.001*). The regression equation (Y = 707.42 + 2.75 X) indicates that every 1 g/cm increase in BMI will be followed by an increase in BP of 2.75 mmHg. The analysis of R-square = 0.942 shows that the accuracy of the linear equation model is 94.2%. In conclusion, BMI has a strong positive correlation with BP, an increase in BMI will increase BP; obesity induces high blood pressure in rats
THE EFFECTIVITY OF CYSTEAMINE SUPPLEMENTATION ON IMPROVING THE IN VITRO FERTILIZATION RATE OF SHEEP OOCYTES
Full text linkThe purpose of this study was to evaluate the effectiveness of cysteamine supplementation on the maturation medium and/or in vitro fertilization medium with regards to improving the normal fertilization rate of sheep oocytes, which are characterized by the formation of two pronuclei. Grade A and B oocytes were matured in medium-199 with 0.3% bovine serum albumin (BSA), 10 IU/mL follicle stimulating hormone (FSH), 10 IU/mL human chorionic gonadotrophin (hCG), and 50 g/mL gentamicin added for 24 hours in a 5% CO2 incubator at 39 C. The treatment group was divided into the following groups: a control group with no cysteamine (P1), a group with 100 M cysteamine in the maturation medium (P2), a group with 100 M cysteamine in the fertilization medium (P3), and a group with 100 M cysteamine both in the maturation and fertilization medium (P4). The fertilization was carried out by incubating sperm-oocytes for 12 hours and then staining them with 1% aceto-orcein to observe the formation of a pronucleus. Normal fertilization rates obtained by each treatment group were 56.5% (P1), 57.1% (P2), 57.8% (P3), and 59.9% (P4) with no significant difference (P0.05) between groups. It was concluded that 100 M cysteamine supplementation in both the maturation medium and fertilization medium was not able to increase the normal fertilization rate of sheep oocytes
Escherichia coli CONTAMINATION AND ITS RESISTANCE TO ANTIBIOTICS IN SEI MEAT
Full text linkThe purpose of this study was to obtain Escherichia coli isolates from 11 sei meat sellers in Kupang City and to observe their resistance to13 types of antibiotics. Escherichia coli were isolated and identified based on SNI 2897: 2008. Antibiotic resistance of the Escherichia colii isolates was determined using the Kirby-Bauer method. The results showed that 13 isolates (39.39%) of Escherichia coli in sei meat samples had a high level of resistance to erythromycin (100%), tetracycline (76.92%), and doxycycline (61.54%). Isolates demonstrated increased resistance to streptomycin (46.15%), cephalothin (38.46%), trimethoprim-sulfamethoxazole (38.46%), amoxicillin (30.77%), chloramphenicol (30.77%), and choline sulfate (30.77%). The antibiotics nalidixic acid and ciprofloxacin demonstrated low Escherichia coli resistance (7.69%). Escherichia coli are sensitive to cefotaxime and gentamicin. A total of 12 isolates (92.31%) experienced MDR. The presence of non-MDR and MDR resistant Escherichia coli in sei meat can seriously threaten community health
HONEY SUPPLEMENTATION IN LACTATE RINGER-EGG YOLK EXTENDER ON QUALITY OF PELUNG CHICKEN SPERMATOZOA POST-CHILLING
Full text linkThe purpose of this research was to determine the influence of honey supplementation in lactate ringer-egg yolk extender with 0.025% sodium dodecyl sulfate and 2% vitamin E addition (LREYSE) on the quality of Pelung chicken spermatozoa preserved at 5 C for 72 hours.Semen was collected from three Pelung chickens once per day over a course of three days using the dorsal-abdominal massage method. Semen was divided into 5 treatment groups of honey supplementation that are 0% as control (LREYSEH0), 1% (LREYSEH1), 2% (LREYSEH2), 3% (LREYSEH3), and 4% (LREYSEH4). This liquid semen was observed for sperm motility and viability every 12 hours. Complete random designrepeated measurement with 4 replications was used in this study. The results showed the motility and viability of spermatozoa in LREYSEextender with 2% honey supplementation (61.251.25% and 71.500.74%) was significantly higher (P0.05) than other treatments that are 0% (51.251.25% and 61.881.36%), 1% (52.501.44% and 63.251.38%), 3% (51.251.25% and 61.631.48%), and 4% (50.002.04% and 60.632.29%) of honey supplementation in extender at 36 hours of storage until the end of the observation at 72 hours of incubation. According to the results of this study, it can be concluded that the 2% honey supplementation in extender is the best treatment to maintain sperm motility and viability for 72 hours of storage
ULTRASOUND IMAGING OF THE SPLEEN, STOMACH, LIVER, KIDNEYS, BLADDER, AND PROSTATE IN PIGLETS (Sus scrofa domestica)
Full text linkThis study aims to describe ultrasonographic imaging of the spleen, stomach, liver, kidney, bladder, and prostate in piglets (Sus scrofa domestica). Ultrasonography was performed on six male piglets aged 1 month with a body weight of 4-8 kg. Ultrasonography of the spleen, stomach, liver, urinary bladder, and prostate was observed in the dorsal-recumbency position, and the kidney was observed in the lateral-recumbency position. The ultrasound examination used the Chison EBit 60, which is a linear type transducer with a frequency of 9 MHz, transverse and sagittal positions, and a gain of about 55-80. Ultrasonography of the spleen showed that the shape of the spleen head varied in each individual. The results of gastric ultrasonography showed the gastric lining clearly and the similarity of the position, shape, structure, echogenicity, and size of the stomach. The results of liver ultrasonography showed that the echogenicity of the liver tended to be hyperechoic, the kidneys were hyperechoic, and the bladder was hypoechoic due to the use of improper gain. The average length of the right kidney was 4.730.50 cm and the left kidney was 4.080.26 cm. The bladder wall thickness was 1.30.31 mm. Imaging of the bladder showed changes in wall size and echogenicity. Prostate ultrasound results obtained an average length of 2.680.45 cm, width 1.690.35 cm. The results of this study can be used as a reference for normal data on organ position, organ structure, and organ echogenicity of the spleen, stomach, liver, kidney, bladder, and prostate in piglets