Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences
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COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL
Get PDFThis study aimed to determine whether the variant or biotype of Trypanosoma evansi can be seen from their polypeptide profiles using 12%sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) stained with Brilliant Blue Commasie. The results generally showed thatthe molecular weight (MW) of polypeptides from nine isolates from East Java, Central Java, Banten, South Kalimantan, Central Kalimantan, andLampung provinces were in the range of 85.46 to 15.76 kD and each isolate has different polypeptide profile. Isolates A13 and A14 were isolatedfrom the same place but have different polypeptide profiles. Likewise, isolates S13 and S18 also have different polypeptide profiles despite beingisolated from the same place at the same time. On the other hand, isolate 372, 87, and 06 have different protein profiles but was classified in thesame biotype namely biotype I. Generally, the difference in protein profile actually more related to the biological diversity of the metabolism ofeach Trypanosoma evansi isolate from Indonesia
THE IMAGE OF EMBRYO AND FETAL OF ETAWA CROSSBRED GOATS USING ULTRASONOGRAPHY AT DIFFERENT GESTATIONAL AGES
Get PDFThis study aimed to estimate the gestational age of Etawa crossbred goats based on ultrasound images of embryo and fetal usingtranscutaneous ultrasonography. Etawa crossbred does (n= 5) were synchronized using double PGF2 injection with 10 days interval and matednaturally. Early pregnancy was diagnosed on 20th day after mating base on isoechogenic image of the embryo (2.650.75 mm) andhypoechogenic appearance of gestational sac. On 30th day of the first trimester, the embryo size was 8.310.42 mm. The embryo reached10.440.51 mm on 35th day. On 60th day, the existence of the fetal were clearly appeared along with isoechogenic of umbilical cord,hyperechogenic os cranium (25.50.1 mm), and body length (51.831.59 mm). On 75th day, the diameter of the os cranium was 33.030.20 mm,body length of 1101.73 mm, and bones structures appeared hyperechogenic for os thoracalis and os vertebrae. The placenta was isoechogenoicwith diameter of 23.30.2 mm. On 90th day, the placenta dominated the uterus (22.671.70 mm) and reached its maximum diameter on 145th day(33.732.91 mm). In conclusion, early pregnancy detection of Etawa crossbred goats using transcutaneous ultrasonography could be performedon 20th day after matin
MUCOLYTIC ACTIVITY OF ROSELLE (Hibiscus sabdariffa L.) CALYCES EXTRACT ON COW INTESTINAL MUCUS
Get PDFThis study aimed to examine the mucolytic activity of roselle calyces extract on cow intestinal mucus in vitro. The extraction of rosellecalyces was performed by the subsequence maceration method using different polarity solvent (n-hexane, ethyl acetate, and methanol). Mucolyticactivity of each extract was evaluated by measuring the viscosity of mixture of cow intestinal mucus and phosphate buffer pH 7 compared toacetylcysteine as the standard mucolytic drug. The result showed that the decreasing of cow intestinal mucus and phosphate buffer pH 7 mixturesviscosity was indicated the increasing of mucolytic activity. The n-hexane, ethyl acetate, and methanol extract of roselle calyces exhibit dosedependentmucolytic activity. Mucolytic activity of roselle calyces ethyl acetate extract with dose of 0.8% and roselle calyces methanol extractwith dose of 0.6% showed no significant difference to mucolytic activity from acetylcysteine with dose of 0.1% (positive control). An increaseddose of roselle calyces extract will decrease the viscosity of cow intestinal mucus and phosphate buffer pH 7 mixtures, indicating an increase inmucolytic activity
THE ROLE OF GUAVA FRUIT FILTRATE ON MAINTAINING THE PRE-FREEZING AND POST-THAWED QUALITY OF BALI BULL SPERMATOZOA
Get PDFThis study aimed to determine the role of guava fruit filtrate (GFF) on maintaining the pre-freezing and post-thawed quality of Bali bullspermatozoa in tris-egg yolk (TEY) extender. The bovine semen was collected using artificial vagina twice a week (n= 10). Ejaculate sampleswere divided into four tubes and each tube was respectively added the following diluents: TEY, TEY + 5% GFF, TEY + 10% GFF, and TEY +15% GFF. The diluted semen was filled into the straws (0.25 mL) and cooled in refrigerator at 4 C for 2.5 hours. The samples were kept onvapor phase of liquid nitrogen (N2) (-120 C) for 10 minutes prior to be stored in liquid phase (-196 C). The quality of pre-freezing and postthawedspermatozoa in straw sample (i.e. motility, viability, and abnormality) was evaluated under light microscopy at 400x magnification. Datawere analysed statistically. The percentage of motility and viability both on pre-freezing and post-thawed process were significantly higher insemen diluted with TEY + 10% GFF compared to control (TEY), TEY + 5% GFF, and TEY + 15% GFF. Addition of 10% GFF into tris-egg yolkextender play role for maintaining the quality of pre-freezing and post-thawed Bali bull spermatozoa
MOLECULAR DETECTION OF GENE RESISTANT TO VARIOUS INSECTICIDES IN Aedes aegypti AT BANYUWANGI EAST JAVA USING POLYMERASE CHAIN REACTION
Get PDFThe purpose of this study was to determine the type of insecticides which Aedes aegypti mosquitoes are resistant to, so that other susceptibleinsecticides still can be used. The study was a cross sectional epidemiological study with cluster sampling in Sub-district Wongsorejo,Banyuwangi, Muncar, Tegaldlimo, Kalibaru which is considered sufficient to represent Banyuwangi Regency. Mosquito samples appropriate tothe characteristics were isolated and tested for insecticide-resistance primer namely voltage gated sodium channel (VGSC) to determine thespecific resistance expressed in mosquitoes. The result of resistance test using WHO standard method showed that Aedes aegypti mosquito fromBanyuwangi Regency were resistant to cypermethrin 0.25% and malathion 0.8%. There was a VGSC coding gene with 250 bp band detectedusing polymerase chain reaction (PCR) technique which was associated with the resistance of Aedes aegypti mosquitoes in Banyuwangi Regencyto organophosphate insecticides (malathione) and pyrethroid insecticides (cypermethrin)
TOTAL BACTERIA AND IDENTIFICATION OF PROTEOLYTIC RUMINAL AND RETICULUM BACTERIA FROM LOCAL KACANG GOAT
Get PDFThis study aimed to calculate the total bacteria and to identify the proteolytic bacteria in rumen and reticulum of local Kacang goat. The samples used were the rumen and reticulum fluids of five goats at abattoirs in Banda Aceh. Isolation of proteolytic bacteria was carried out using skim milk agar with pour plate method and incubated at 39 C for 48 hours. The bacterial colonies morphology was observed and the total bacterial count was recorded. DNA of the widest proteolytic index colony was isolated, amplified, and sequenced. The results showed that the dominant colonies morphology was white with position inside the agar. The average of total proteolytic bacteria in goat rumen fluid and reticulum fluid were 6.416x106 CFU/mL and 2,382x107 CFU/mL, respectively. Isolates with the widest proteolytic index was Ru3 (2.5 mm) in the rumen which homology and phylogenetic tree analysis of 16S rRNA showed 93% sequence similarity with Bacillus subtilis, while in reticulum was Re1 (2.0 mm) which has 92% sequence similarity to Tatumella. It is concluded that the number of proteolytic bacteria in reticulum is greater than the number of proteolytic bacteria in the rumen of local kacang goat. Homology analysis in this study proved that the Ru3 and Re1 isolate were probably either a new species or unconfirmed species
THE DILATATION OF BRAIN VENTRICLE DUE TO CONGENITAL TOXOPLASMOSIS IN MICE CORRELATED WITH APOPTOSIS BUT NOT WITH TRANSFORMING GROWTH FACTOR BETA
Get PDFThis study aimed to determine the occurences of mice brain ventricles dilatation that congenitally infected with Toxoplasma gondii (T. gondii) as a marker of hydrocephalus and cellular changes in the brain. A total of twenty pregnant mice (11.5 days pregnacy) were divided into 2 groups, which were control (P1) group and treatment (P2) group. The mice in the treatment group were infected with 101 tachyzoites of T. gondii. All mice were maintained until delivery. The newborn mice were sacrificed and their brain were removed and fixed in 10% buffered formalin to prepare histology slides with HE staining for observation of ventricular width, TUNEL assay for apoptosis observation, and immunohistochemistry for the expression of transforming growth factor beta (TGF-) observations. The data were analyzed using t test and linear regression. The results showed that ventricular width and apoptosis index significantly increased (P0.01) in the treatment group compared to control group, but there was no difference in the expression of TGF- (P0.05) in both groups. Dilatation of ventricle correlated with the apoptotic index of brain cells but did not correlated with the expression of TGF-
THE POTENCY OF PULUTAN (Urenalobata L.) LEAVES DECOCTION AS ANTIFERTILITY BASED ON ITS EFFECT ON UTERINE DEVELOPMENT OF BALB C MICE (Musmusculus)
Get PDFThe purpose of this research was to determine the effect of decoction of pulutan leaves simplicia(Urenalobata L.) on the development of the uterus of Balb C mice (Musmusculus). Female mice aged 10-12 weeks were given decoction of pulutan leaves simplicia with concentration of 5%, 7.5%, 10%, 12.5%, 15%, and control. The administration of pulutan leaves was performed by gavage for 11 consecutive days. On day 12, mice were dissected, then, the uterus was taken for histological preparations using paraffin method. Data such as uterine diameter and myometrium and epimetrium thickness were analyzed using one way analysis of variance followed by least significant different. The results showed that decoction of pulutan leaves simplicia decrease the uterine diameter and epimetrium and myometrium thickness compared to control. The decrease of uterine diameter and uterine myometrium and epimetrium thickness was started at the concentration of 7.5%. Decoction of pulutan leaves simplicia could reduce uterine diameter and thickness of uterine myometrium and epimetrium, thus, it might decrease the uterine function as the site of implantation. The decline in the function of the uterus might decrease the number of born children, thus decoction of pulutan leaves simplicia is potential as antifertility agent
Effect of PMSG administration in combination with vaginal sponge on estrous occurrence and litter size of Javanese sheep
No full textAbstractThe use of vaginal sponge in estrous synchronization has been a popular method to uniform estrous cycle of small ruminants. In order to increase the litter size, the use of the sponge is combined with PMSG. Therefore the objective of this study was to evaluate the effect of PMSG in combination with vaginal sponge on estrous response and litter size of Javanese local sheep. The study was conducted in Faculty of Animal Husbandry, Padjadjaran University, Indonesia with 32 non-pregnant local sheep. All these sheep was divided into four groups of treatment, i.e: 1) group of sheep was subjected to vaginal sponge (VS) treatment containing 30 mg Medroxyprogesterone acetat (MAP) inserted in the vagina for 14 days; 2) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection -1 d before VS removal; 3) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection at the day of VS removal; 4) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection +1 d after VS removal. The result showed that all treatment groups had 100% of estrous response. However, the occurrence of estrous time varied among group after VS removal. Group 1 (G1) and G4 tend to have estrous occurrence at day 3 after VS removal by 55% and 75% response, respectively. Whilst, G2 and G3 tend to have estrous occurrence respectively at day 1 (62,5%) and day 2 (50%) after VS removal. A 100% of pregnancy rate occurs in G2 and G4, while G1 and G3 have a 88,9% and 87,5%, respectively. No significant effects of the treatments on the ewes litter size. In conclusion, the use of PMSG administration 24 h prior, after, or at sponge withdrawal increase a tighter synchrony of oestrous and pregnancy rate, but has no effect on the increasing of litter size in Javanese local sheep.Keywords: Vaginal sponge, PMSG, estrous response, pregnancy rate, litter size, shee
THE EFFECTIVENESS OF PROGESTERONE HORMONE OR PGF2 AND hCG APPLICATION TO INCREASE REPRODUCTIVE EFFICIENCY OF POSTPARTUM OF ANESTRUS PE GOATS
Get PDFThis study aimed to improve reproductive condition of postpartum anestrus of Etawa crossbreed (PE) goats using PGF2 hormone or progesterone (controlled internal drug release/CIDR-G) and improve pregnancy success from artificial insemination (AI) with human chorionic gonadotrophin (hCG) hormone administration. Administration of progesterone increased estrus by 100%, which was higher than the 70% produced from prostaglandin F2 alfa (PGF2) administration. Estrus onset in CIDR group (35.8013.33 hours) was faster than PGF2 group (45.69.2 hours). Clinical signs of vulva (redness and swollen) were more intense in CIDR-G group (80% and 70%, respectively) compared to PGF2 group (70% and 57%, respectively). Estrus duration in PGF2 group (52.173.34 hours) was longer than CIDR-G group (49.613.56 hours). Pregnancy success in group given hCG was 66.7% while in non-hCG group was 50%. In conclusion, CIDR-G and PGF2 administration is effective to induce estrus of postpartum anestrus PE goats. Administration of hCG at the onset of estrus is effective to improve pregnancy success