BIOLINK : Jurnal Biologi Lingkungan, Industri dan Kesehatan
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EFFECT OF COMBRETUM INDICUM LEAF EXTRACT ON BLOOD SUGAR LEVELS AND PANCREAS HISTOLOGY OF ALLOXAN INDUCED MICE
Get PDFDiabetes is a health problem that affects more than half a billion people in the world. Several plants are known to have anti-diabetic activity, one of which is Dutch jasmine (Combretum indicum). This research was conducted to determine the effect of Combretum indicum leaf extract on blood sugar levels and histology of islets of Langerhans in mice. A total of 36 male mice aged 6-8 weeks were divided into 6 treatment groups, namely normal control (KN) without treatment; negative control (K) with alloxan treatment; positive control (K+) with alloxan and glibenclamide treatment; P1 treated with alloxan and Combretum indicum leaf extract at a dose of 100 mg/kgBW; P2 with treatment with alloxan and Combretum indicum leaf extract at a dose of 200 mg/kgBW; P3 with alloxan treatment and Combretum indicum leaf extract at a dose of 200 mg/kgBW. Mice that have been induced by alloxan will be treated with leaf extract and glibenclamide for 20 days. Blood sugar levels 3 days after alloxan induction were checked and checked again on the 10th and 20th days of treatment by taking blood through the blood vessels in the mice's tails and then measuring them using a glucometer. The pancreas was dissected on the 20th day and pancreatic histology preparations were made for histological analysis. The results of this study indicate that Combretum indicum leaf extract has antihyperglycemic effects by protecting islets of Langerhans cells from alloxan-induced oxidative stress
EFFECTIVENESS OF LOTUS PHYTOREMEDIATION (NYMPHAEA PUBESCENS) IN REDUCING AMMONIA AND PHOSPHATE CONCENTRATIONS IN COFFEE LIQUID WASTE
Get PDFBased on laboratory tests of ammonia and phosphate concentrations in coffee wastewater from coffee factory in Semarang, the concentrations were found to be 8.55 and 1.01 mg/L, respectively. These high concentrations negatively impact fish farming production. Nymphaea pubescens has shown potential in remediating ammonia and phosphate concentrations in coffee wastewater. Therefore, the objective of this study is to determine the effectiveness of lotus phytoremediation (Nymphaea pubescens) in reducing ammonia and phosphate concentrations in coffee wastewater. The research method employed is true experiment with Completely Randomized Design, involving four treatments and three replications. According to ANOVA and Duncan's Multiple Range Test results, Nymphaea pubescens significantly reduced ammonia and phosphate concentrations in coffee wastewater. So far there have been no specific studies on the phytoremediation of Nymphaea pubescens for ammonia and phosphate concentrations in coffee wastewater, making this study a novel contribution. The findings suggest that this approach should be widely implemented, especially within educational settings and communities
THE POTENTIAL UTILIZATION OF RASAU FIBER (Pandanus helicopus) AS AN ALTERNATIVE NON-FOOD INDUSTRY MATERIAL TOWARDS A CREATIVE ECONOMY
Get PDFPandanus helicopus is one of the fibrous plants that is considered a nuisance so that its utilization is still lacking. Meanwhile, P. helicopus's fiber has the potential to be used in industry, especially in the manufacture of food brushes. The objective of this study is to identify the potential of P. helicopus's fiber, which is used as an alternative to non-food industrial materials, especially brushes that are halal toward the principle of creative economy. This study is experimental research by combining two methods, there are exploration and experiment laboratory. The results showed that P. helicopus leaf fibers have the characteristics of fibers that are quite fine, strong and elastic. Based on the laboratory tests, it was found that fibers became stronger and finer when given immersion treatment in NaOH solution with the most effective concentration of 7.5% with the test results of a fineness level of 2462.88 Tex, a tenacity value of 145.07 mN/Tex and 14.80 gf/Tex, and fiber elasticity of 19.56%. The characteristics of P. helicopus leaf fiber has the potential to be used as an alternative material in the industrial field, especially in the manufacture of halal plant fiber brushes. The utilization of P. helicopus's fiber in industry can be a creative innovation that can realize the principles of creative economy
INSECT DIVERSITY IN MANGROVE ECOSYSTEMS IN PAYUM BEACH, MERAUKE REGENCY, PAPUA
Get PDFMangrove forest is a permanent habitat and transit area for various fauna. In the mangrove community, it forms a mixed habitat between terrestrial and aquatic fauna groups. This study aims to analyze the composition of mangrove species and the abundance of insects. There are 3 research station on the mangrove forest ecosystem on Payum beach. Sampling was carried out using the Line Transect method, starting with making a line transect (50 meters) on the outermost mangrove stands and pulling them inland. On each transect line a plot measuring 10 x 10 m is placed, in each of which 5 subplots/quadrants measuring 1 x 1 m are placed. Each transect line consist of 3 plots and 15 sub-plots will be obtained, so there are a total of 45 sub-plots. Based on the research results, it was found that several types of macrobenthos live abundantly and are associated with mangrove ecosystems, such as Terebralia palustris, Terebralia semistriata, Nerita sp., Cassidula angulifera, Cerithidea sp., Telescopium telescopium. Four species of mangroves are found on Payum Beach, Merauke Regency, South Papua Province, namely Avicennia alba, Rhizophora apiculate, Sonneratia alba, and Aegialitis annulata. The insect diversity index in the mangrove forest on Payum Beach in Merauke is H'=2.61, which indicates that the environmental conditions of Payum Beach are still quite good and insect diversity is in the medium category. The evenness index of 0.42 is included in the medium category because human activities vary, and the dominance index is 0.63 because the Payum Beach mangrove forest has various types of insects with moderate dominance
ANTIBIOFILM ACTIVITY OF MANGOSTEEN (Garcinia mangostana L.) LEAF EXTRACT AGAINST COLLECTION OF BACTERIAL ISOLATES FROM DIABETIC ULCERS
Get PDFMangosteen (Garcinia mangostana L.) is a plant that contains many benefits and has the potential to be a medicinal plant in treating various diseases. Mangosteen leaves contain flavonoids, saponins, tannins, alkaloids and terpenoids which are known to have antibacterial and antibiofilm properties. This anti-biofilm agent is an alternative treatment for diabetic wound infections where cases of antibiotic resistance have increased. One of the causes of resistance is the biofilm formed by infectious bacteria. This research is a type of laboratory experimental research which aims to determine the anti-biofilm activity of mangosteen leaf extract against biofilms formed by a collection of bacterial isolates from diabetic wounds. The antibiofilm activity test carried out consisted of a cell attachment prevention test, a biofilm formation inhibition test, and a biofilm destruction test using the crystal violet staining method using the tube method and measuring optical density values on a UV-Vis spectrophotometer. The concentrations of mangosteen leaf extract used are 60%, 80% and 100% as well as control – and control +. The results of the antibiofilm test showed that mangosteen leaf extract had the best activity in inhibiting biofilm formation, preventing cell attachment, and destroying biofilm, respectively. The three most optimal activities were found at a concentration of 100%, with an inhibition percentage of 50.51%, prevention of 32.56%, and destruction of 5.63%
TOXICITY OF ETHANOL EXTRACT OF PELAWAN LEAVES (TRISTANIOPSIS OBOVATA Benn.) ON THE HEMATOLOGICAL PROFILE OF WHITE RATS (RATTUS NORVEGICUS L.)
Get PDFTristaniopsis obovata Benn. is one of the herbal plants that contains phenolics flavonoids, saponins, tannins and steroids/triterpenoids. The secondary metabolites are known to have antioxidant activity and toxicity that can affect hematological profiles. This study aims to determine the toxicity effects of ethanol extract of T. obovata Benn. leaves on LD50 value, erythrocyte morphology, and hematological profile in white rats. The study used a Complete Randomized Design (CRD) with 4 treatments and 4 repeats. The treatment consisted of P0 (control), P1 (dose of 300 mg/kg body weight) (P1), P2 (dose of 2000 mg/kg body weight), and P3 (dose of 5000 mg/kg body weight). LD50 calculation using AOT 425 StatPgm software and hematological profile analysis using ANOVA. The results showed LD50 value of T. obovata Benn. leaf extract. was 1.750 mg/kg BW as mild toxic. The morphology of P2 erythrocytes shows abnormal form anisocytosis and P3 form Burr cells and macrocytosis. The administration of extract in all treatments was not significant from the number of erythrocytes, leukocytes and hemoglobin comcentration of white rats
ANALYSIS OF MINERAL CONTENT IN ZAM-ZAM WATER CIRCULATION IN PADANGSIDIMPUAN CITY
Get PDFThis research was motivated by the existence of Zam-zam water trade in Padangsidimpuan City, while the Saudi Arabian government prohibited the export of Zam-zam water. This causes people to suspect that the quality of Zam-zam water circulating in the market is not the same as the Zam-zam water found in Mecca. Therefore, research needs to be carried out to answer these concerns. This research aims to analyze the mineral content of labeled packaged Zam-zam water circulating in Padangsidimpuan City compared with Zam-zam water brought from Mecca City. The mineral concentrations tested were sodium and chloride. This research uses laboratory experimental methods, including quantitative analysis. According to the type of research, the data analysis technique used is to compare the concentration of each mineral in the water labeled Zam-zam with the mineral concentration in the Zam-zam water used as a control. The research results showed that Zam-zam water obtained from Mecca City contained 42.4 mg/L chloride, 2.06 mg/L sodium, while the labeled Zam-zam water circulating in Padangsidimpuan, namely sample 1, contained 160.7 mg/L chloride. sodium 2.06 mg/L in sample 2 contains 50.5 mg/L chloride, sodium 2.17 mg/L. The three Zam-Zam samples circulating in Padangsidimpuan that have been tested show that the quality of Zam-Zam water is suitable for consumption based on determining a good water quality test, namely at the suitability threshold
UTILIZATION OF BIOMASS WASTE WITH STEARIC ACID AS FIRE STARTER
Get PDFFire starter is a product used to ignite a flame, particularly when using firewood, and is considered a potential alternative solution for managing biomass waste in Indonesia. This study aims to determine the quality of fire starters made from sawdust, pine cones, and dry leaves, using stearic acid as an adhesive. The research consists of three stages: production of fire starter, quality testing, and data analysis. The ratios of biomass to stearic acid used in this study are 3:8, 2:7, and 1:6. The quality tests conducted include ignition time, burn duration, burning rate, flame height, and durability test. The results indicate that in the ignition time test, the fire starter made from dry leaves had the fastest ignition time (17 seconds). The burn duration test showed that sawdust was the best material (1,423 seconds). The burning rate test found that dry leaves had the best result (0.035 g/s). The flame height test revealed that sawdust produced the tallest flame (35 cm). For the durability test, all the fire starters had the same durability value (100%)
RESULTS OF ECOPRINT MOTIFS BASED ON DIFFERENCES IN TYPES OF FABRIC, LENGTH OF TIME, TYPES OF MORDANTS, AND TYPES OF NATURAL DYES
Get PDFBiodiversity of flora in Indonesia greatly supports the development of textile products, ecoprint is one of the textile products that involves the utilization of this diversity. Various types of plants found in the surrounding environment can be used as dyes for making ecoprints. Ecoprint is a color transfer technique on fabric which in the manufacturing process requires a mordant to bind plant colors to the fabric. This study aims to determine the effect of variations in fabric type, length of time, type of mordant, and type of natural dye on ecoprint color expression. This experiment used the steaming technique in ecoprint to transfer motifs and colors to fabric. In this study, three types of fabric were used, namely: toyobo cotton, mori, and blanco. Variants of steaming time were tested at intervals of 60 minutes, 90 minutes and 120 minutes. Variations in the types of mordant used are vinegar and alum. Meanwhile, the types of natural dyes used are jolawe, soga, and secang. The results of the research show that there are differences in color expression produced by vinegar and alum mordant substances. Alum mordant produces intense color expression and clear form. Leaf motifs can be printed well on toyobo cotton fabric. Variations in steaming time in this study did not produce significant differences in color expression. Meanwhile, each natural dye produces a different color expression
CONSUMER KNOWLEDGE, ATTITUDE, ENVIRONMENT AND INCOME TOWARD COOKING OIL WASTE
Get PDFUsed cooking oil is waste oil that comes from a type of cooking oil that has been used repeatedly. If not managed properly, it can become a source of environmental pollution. Managing used cooking oil still needs to be in a good category. Preliminary studies from this research found that as many as 60% of people in the Cengkareng Village area, West Jakarta, need to manage used cooking oil properly. This research aims to determine factors related to knowledge, attitudes, income and environment in managing used cooking oil. The sample used was 141 homemakers using purposive sampling. The data collection methods used were interviews and observation. This research uses independent variables (Knowledge, Attitudes, Income Level, and Environment) and dependent variables (Used cooking oil management behaviour). The analysis used is descriptive analysis with the chi-square test. Based on the research results, it was found that the highest proportion of used cooking oil management behaviour was poor (51.1%), good knowledge (54.6%), negative attitude (60.3%), sufficient income level (81.6%), and bad environment (93.6%). %). There is a relationship between knowledge (p-value = 0.001) and community behaviour in managing used cooking oil and a relationship between attitude (p-value = 0.001) and behaviour in the community driving used cooking oil. There is a need for interaction and cooperation between societal stakeholders to improve public awareness of cooking oil management to create a zero-waste environment