BIOLINK : Jurnal Biologi Lingkungan, Industri dan Kesehatan
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THE INFLUENCE OF DIFFERENT GROWING MEDIA ON THE GROWTH OF WATER SPINACH (IPOMEA REPTANS Poir) USING AQUAPONIC TECHNOLOGY
This research aims to determine the best-growing medium to support the growth of water spinach plants using aquaponic technology. The study used a non-factorial Completely Randomized Design (CRD) method. There were four types of planting media treatments, namely, rockwool (control), cocopeat (P1), pasir malang (P2), and burnt husk (P3), there were five growth parameters observed (plant height (cm), number of leaves, stem diameter (mm), wet weight (gr), and leaf color) were monitored once a week starting from 7 HST to 28 HST. Data were analyzed using variance at a confidence level of 5% and continued with the Duncan test (DMRT) at a level of 5%. The results showed that the type of growing medium affects the growth of water spinach. pasir malang provided the best results for all plant parameters. However, it was not significantly different from burnt husk, except for stem diameter, where pasir malang differed significantly from the other treatments. Rockwool, pasir malang, and the burnt husk produced better and healthier leaf colors compared to cocopeat
INVENTORY OF INVASIVE INSECT SPECIES IN COBAN TALUN, BATU CITY
Insects are the greatest number of animal species on earth. Invasive species are insects that are non-native to a particular area, and negatively affect other organisms in their ecosystem. This study examines the diversity of invasive insect species with the aim of determining the diversity of invasive insect species found in Coban Talun, Batu City, effective and sustainable invasive insect control strategies, as well as conservation actions that need to be taken to maintain the balance of the ecosystem in Coban Talun. This study used a descriptive method using exploration techniques at three observation locations to collect data. This method used was observation of the focus of the problem under study, namely invasive insects which are the object of research. The data collection technique was carried out by exploring the location under study by observing, documenting, describing and identifying specimens using various literature. The results of the study obtained were the discovery of several types of invasive insects including black cockroaches (Phyllodromica maculata), large heath butterflies (Coenonympha tullia), green grasshoppers (Tettigonia viridissima), flies (Musca domestica), Mexican bean beetles (Epilachna varivestis), and soldier beetles (Rhagonycha recta). Invasive types of insects need population control by using natural insecticides so that the ecosystem in Coban Talun remains balanced
IDENTIFICATION OF ESCHERICHIA COLI AND ORGANIC SUBSTANCES BASED ON THE DISTANCE FROM THE DUG WELL TO THE SEP-TIC TANK
As many as 70% of drinking water sources in Indonesia are contaminated with feces which can cause the spread of diarrheal diseases. There were 1,177 cases of diarrhea spread across Colomadu District. In Pucung Village, Colomadu District, complaints about well water being colored, smelly, tasteful, slimy, and people often experiencing diarrhea, especially those located less than 10 m from the septic tank. In accordance with Minister of Health Regulation Number 32 of 2017 concerning Water Hygiene, the E. coli content is 0 CFU/100 ml and the maximum organic material content is 10 mg/l. The E. coli analysis method uses Total Plate Count (TPC) on agar plates according to SNI 2897:2008. Research needs to be carried out to ensure the quality of well water regarding the content of E. coli and organic substances. Determination of organic substance levels was carried out using the permanganometric titration method according to SNI 06-6989.22-2004. This research uses the TPC method to determine water quality and is included in qualitative research which focuses on case studies. Based on the research, the results showed that the values of organic substances and E. coli in sample I were 17.20 mg/l and 1.6 x 109 CFU/100 ml, sample II was 11.88 mg/l and 5.0 x 105 CFU / 100 ml, while sample III was 6.88 mg/l and 2.3 x 104 CFU/100 ml. So, the quality of the well water in the Pucung Village studied did not meet the requirements of Minister of Health Regulation Number 32 of 2017.
EVALUATION OF THE WASTE WATER TREATMENT INSTALLATION OF THE SELE BE SOLU REGIONAL PUBLIC HOSPITAL SORONG CITY
The Hospital Wastewater Treatment Plant (WWTP) is a system of hospital wastewater treatment designed based on the characteristics of waste water entering from several sources of waste discharge. The research location is at Sele Be Solu Regional Hospital, Sorong City, West Papua. The land owned by Sele Be Solu Regional Hospital is approximately 120,000 m2, and the number of beds is 158. This study uses laboratory tests to measure the quality of wastewater and the wastewater treatment plant. From the calculated results, the maximum discharge produced by Sele Be Solu Regional Hospital is 4343 m3/month. With the actual pollution load for the BOD parameters of 26.36 kg/month, COD of 157.98 kg/month, and TSS of 38,343 kg/month, all three are greater than the maximum pollution load allowed, namely for the BOD of 12,708 kg/month, COD of 105.9 kg/month, and TSS of 211.8 kg/month. The quality of wastewater from the Sele Be Solu Regional Hospital's wastewater treatment plant in 2017, which was tested by PT. Kehati Lab Indonesia, showed that all parameters met the quality standard requirements of the Minister of Environment Regulation Number P.68 of 2016. Meanwhile, the quality of processed wastewater in 2019 and 2021 showed that the parameters for Coli Group germs exceeded the permitted quality standards, while other parameters still meet the quality standards
An IN VIVO EVALUATION OF COMBRETUM INDICUM FLOWER METHANOLIC EXTRACT AS OINTMENT FOR BURN WOUND ON MALE MICE
Burns that are not treated properly can be dangerous, even can lead to death. Combretum indicum is reported to have anti-inflammatory, antibacterial, and high antioxidant activities, making it effective in healing dermal wounds. This study aimed to evaluate the wound healing effect of C. indicum flower methanolic extract on burn wound model in male mice. A total of 36 adult male mice were randomly assigned to 6 groups namely Negative control (I) without treatment; Positive Control I (II) with Burnazin treatment; Positive control II (III) with Vaseline treatment; Treatment group I (IV) with 25% concentration of C. indicum flower methanolic extract ointment; Treatment group II (V) with 50% concentration of C. indicum flower methanolic extract ointment; Treatment group III (VI) with 75% concentration of C. indicum flower methanolic extract ointment. Mice were treated daily for 20 days, and wound areas were observed every 2 days. Hydroxyproline level and total DNA content were tested on day-11. Skin tissue was analysed histologically. The results indicate that C. indicum flower extract accelerate wound healing process and repair the damaged skin tissue by accelerate the Proliferative phase
EFFECT OF FERMENTATION OPTIMIZATION WITH PH CONTROL ON ACTINOMYCETES ISOLATE AS ANTIBACTERIAL STAPHYLOCOCCUS AUREUS
Actinomycetes are Gram-positive bacilli noted for their secondary metabolites that are effective as antibacterials. Production of the antibacterial compounds is determined by nutrition, temperature, pH, and fermentation time. This study aims to study the antibacterial activity of Actinomycetes isolates from Bogor Botanical Gardens soil by fermentation optimization with pH control on the 3rd, 4th, and 5th day against the growth of Staphylococcus aureus bacteria. Actinomycetes isolates were inoculated on Starch Casein Agar (SCA) and fermented in liquid media containing 7 g glucose, 14 g mannitol, 14 g peptone for 5 days with the pH controlled within the range of 6.2–6.8. Antibacterial test method against S.aureus using well diffusion method with Nutrient Agar (NA) media. The inhibition zone diameter was measured every 24 hours and and the results obtained were 3.73 mm; 6.05mm; and 5.34mm. Those data were analyzed using the Kruskal-Wallis test and then continued with the Mann Whitney test which had significant differences between groups. The conclusion of the study is Actinomycetes isolates have antibacterial potential against the growth of S. aureus with the largest average diameter of inhibition zone in the 4th day fermentation group, which had moderate inhibition
DETECTION OF DANGEROUS FOOD ADDITIVE IN TAKJIL SNACKS DURING OF RAMADHAN
Food is a basic human need, necessary to meet daily needs. Human health is greatly influenced by what they consume. Food products added dangerous ingredients can cause serious health effects for humans. The aim of this research was to detect substances of formaldehyde and borax in snacks in the month of Ramadhan (takjil) at Bekasi City. This was descriptive observational research design by direct examination for formalin and borax from the food samples. The population in this study were all traders who sold Takjil on the roadsides in Bekasi City. Sample selected used an incidental sampling technique by taking 8 food samples which. Based on references, they were proven to contain dangerous chemicals. Based on the research results, 2 food samples were found positive containing dangerous food additives, namely 1 sample of lontong (12.5%) was positive for containing borax and 1 sample of martabak tofu (12.5%) was positive for containing Formalin. Seasonal traders who Sell Takjil food during the month of Ramadan may not necessarily be safe. Therefore, it is recommended that health workers from Puskesmas must frequently inspect the food products and socialize the importance of using safe food additives
BIODIVERSITY OF ANGIOSPERMS IN SUMBERAWAN TEMPLE, SINGOSARI, MALANG DISTRICT
Sumberawan Temple is a tourist area that is inextricably linked to diversity of plants. One of them is angiosperm plants. Angiosperms occupy about 90% of all plant species and have 250,000 species. The objective of this study is to determine the biodiversity of angiosperm plants in Sumberawan Temple area which is located in Singosari, Malang Regency. The research method used was vegetation analysis using a single plot measuring 20 x 20 m2. The shape of the plot used wass square with an area of 400 m2. There were 12 types of angiosperm plant species in 9 different families. To determine the species richness index using the Margalef index, the diversity index using the Shannon Weinner index and the evenness index using the Evenees index. The findings indicated that species richness was in the low category, diversity and evenness were in the high category. It is due to the fewer species in the area but the greater variety of species in Sumberawan Temple area. The diversity of species indicates the number of species that coexist
The EFFECT OF COMBRETUM INDICUM LEAVES ON LIPID PROFILE AND HEMATOLOGY OF MICE INDUCED WITH ALLOXAN
Diabetes has become one of the main causes of death throughout the world recent years. Diabetes symptoms include high blood glucose and hyperlipidemia. An rise in blood glucose can produce an increase in the amount of leukocytes and erythrocytes in the hematology profile. Consequently, this study was to ascertain how extract Combretum indicum leaves affected the lipid profile and hematological of mice given alloxan. The study sample included 36 male mice divided into six groups: normal control (KN) without treatment, negative control (K-) with alloxan treatment, positive control (K+) with alloxan and glibenclamide treatment, P1 with alloxan treatment and Combretum indicum extract dose 100 mg/kgBW, P2 with alloxan treatment and Combretum indicum extract dose 200 mg/kgBW, and P3 with alloxan treatment and Combretum indicum extract dose 400 mg/kgBW. After the mice hyperglycemia on day 3rd, the therapy was continued. The treatment lasted for 20 days. On days 10 and 20, hematological and lipid profile measures were performed. Combretum indicum leaves have the ability to lower total cholesterol, LDL and triglyceride with ascalate HDL levels on 20 days of treatment. In addition, it markedly raised the percentage of lymphocytes and neutrophils with escalate the quantities of lymphocytes and neutrophils in alloxan induced mice. So, in aloxan-induced mice, this extract can reduce the effects of diabetes
ACTIVITY TEST OF BESTSELLING MOISTURIZERS ON SHOPEE IN TREATING SKIN PROBLEMS
Shopee is an e-commerce widely used by Indonesians to buy and sell products and can shop safely. Shopee shows that in 2020, moisturizers was the top selling product in the beauty category. The moisturizers sold have different ingredients, come from many brands, and have different price points. The objective of the study is to compare the 3 best-selling moisturizers in the shopee category that are more effective in improving skin problems. This study was a quantitative study using a true experimental design with a posttest only control group design consisting of 4 groups, namely 3 groups using 3 best-selling moisturizer brands based on the shopee category and 1 control group. The sample of this study was selected based on the inclusion criteria consisting of 20 volunteers and the test was conducted for 4 weeks of application on the volunteers' skin by looking at the measured parameters of moisture, pores, blemishes and wrinkles. The results showed that the activity test of moisturizer A had a faster recovery in increasing moisture content (54.8%), shrinking pore size (23.8%), reducing blemishes (19.9%) and wrinkles 28.9%