BIOLINK : Jurnal Biologi Lingkungan, Industri dan Kesehatan
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DIVERSITY OF PISCES IN THE LEMBAH TUMPANG
Get PDFThe objective of this study was to determine the diversity of fish species found in Tumpang Valley, Tumpang Sub-district, Malang Regency, East Java as an effort to preserve fish species. The method used in this research is observation in various ponds by using the help of reflection book and google lens to identify the types of fish found. Based on the results of research conducted by researchers on the diversity of fish species in Tumpang Resort Valley Area, Tumpang District, Malang Regency, the results indiccate that there are 5 species, namely koi fish, Javanese wader fish (tawes), tilapia fish, citul fish, and goldfish. There are various types of koi fish with different genetic variations, but there are 8 koi fish that can be identified by researchers, namely platinum koi fish, orenji ogon koi fish, karasugoi koi fish, kigoi koi fish, yamato nishiki koi fish, nezu ogon koi fish, silver koi fish, and sanke koi fish. The result of the fish diversity index obtained is 1.173 (in the low category)
THE IDENTIFICATION OF MICROPLASTICS IN GILLS AND DIGESTIVE TRACT IN TILAPIA FISH (Oreochromis mossambicus) IN LAKE TOBA
Get PDFMicroplastics threaten the survival of aquatic organisms including fish in Lake Toba. This study aims to identify the type and abundance of microplastics found in the gills and digestive tract of tilapia (Orochromis mossambicus) in cages and non-cages of Lake Toba, Samosir Regency. Sampling was conducted in February-March 2024 at three stations namely Tanjung Bunga, Palipi, Simarsasar with sampling at each location of 10 caged and non-caged fish each. Extraction of microplastics using the Fenton. Observation parameters included gill weight, gut length, and abundance of microplastics in the gills and digestive tract. The results revealed that the majority of microplastics identified in cages and non-cages were fiber and filament types, with non-cage fish having a higher abundance of microplastics than cage fish
CARBON ABSORPTION POTENTIAL IN MANGROVE ECOSYSTEM IN BORONGKALUKUA, MAROS REGENCY, SOUTH SULAWESI, INDONESIA
No full textMangrove ecosystems are essential in mitigating climate change through Carbon storage in sediments. This study aims to analyze the bulk density values, carbon concentration, and Carbon storage and absorption in mangrove sediments in Borongkalukua, Maros Regency. Sampling was carried out at five sediment depths (0-10 cm, 10-20 cm, 20-30 cm, 30-40 cm, and 40-50 cm) using the Loss on Ignition (LOI) method and analyzed statistically using one-way and two-way ANOVA. The results showed an average bulk density value of 0.33 g/cm³, with the highest value at a depth of 30-40 cm (0.28 g/cm³). Furthermore, the average Carbon concentration was 11.37%, with the highest value at a depth of 40-50 cm (20.50%) and the lowest at 0-10 cm (1.98%), with no significant differences between depths (p-value > 0.05). Meanwhile, Carbon storage in sediment reached 2,423.77 tons/ha. Total absorption reaching 8,895.23 tons/ha. In conclusion, sediment depth affects carbon accumulation, with higher carbon content in deeper layers due to the sedimentation process and minimal decomposition. This study emphasizes the importance of mangrove conservation in supporting Carbon storage and climate change mitigation
THE EFFECT OF ORGANIC BIOFERTILIZER FROM BSF LARVAE (Hermetia illucens) AND LOCAL MICROORGANISM ON THE GROWTH OF CAISIM MUSTARD PLANTS
Get PDFOne of the solutions developed for utilizing organic materials in agriculture is the use of biological fertilizer. This research is different from previous fertilizer research, where the biofertilizer in this study came from empty palm oil bunches, animal waste by using maggots (BSF larvae, Hermetia illucens) and local microorganisms to degrade them. The aim of this research is to obtain the composition of organic biofertilizer from three wastes that have been processed using maggots and local microorganisms. This research method uses RAL with 12 treatments with 3 repetitions. The results of a study of organic biofertilizer from BSF larvae and a consortium of local microorganisms on white mustard plants (Brasica chinensis) showed that treatment with a combination of animal waste, microbes and BSF larvae showed good results on the growth of white mustard plants. This is proven by the results of measuring soil pH, the highest height of chicory plants and the highest number of leaves compared to other organic biological fertilizer treatments, which were produced at the end of the measurement on day 30, the pH in the treatment with that combination is 4, plant height is 33 cm, and the number of leaves is 20
ANALYSIS OF SOIL MACROFAUNA DIVERSITY IN DRAGON FRUIT AREA AT AL-ASRI FRUIT GARDEN APPLIED WITH PALM OIL MILL EFFLUENT
Get PDFThe presence and diversity of soil macrofauna in dragon fruit areas that are not applied with Palm Oil Mill Effluent (POME) and those applied with POME as fertilizer, with variations in application time, can reflect different soil qualities. This study aims to analyze species presence, Diversity Index (H') soil macrofauna, and its correlationwith application POME in dragon fruit areas without application (Block I) and those applied with POME (Pond 5) PT. Anugrah Tanjung Medan, with variations in waste application time 14 days (Block II) and 28 days (Block III). The research used Purposive Sampling method, and soil macrofauna sampling with Quadrat and Hand Sorting methods. The results of the study revealed 52 species of soil macrofauna, with 28 species Block I, 40 species Block II, and 37 species Block III. The highest H' value for soil macrofauna was found in Block II (3.08), categorized as high diversity, followed by Block III (2.87) and Block I (2.72), which were categorized as moderate diversity. These results indicate that utilization of POME as fertilizer for 14 days (Block II) has a positive correlation with increase in the presence and diversity of soil macrofauna, contributing to improved soil quality
IN SILICO AND IN VITRO ANALYSIS OF BLASHV AND BLATEM GENE PRIMERS
Get PDFBacterial pollution that occurs in the environment causes an increase in disease and antibiotic use, which leads to resistance. This study analyzes antibiotic-resistant blaSHV and blaTEM genes in silico and in vitro. Antibiotic-resistant bacterial isolates on ESBL will be carried out PCR and electrophoresis. The type of research used is descriptive observational research conducted from April to September 2024. The sampling technique used was random sampling. Primer blaSHV with sequence F' AGGATTGACTGCCTTTTTG and R' ATTTGCTGATTTCGCTCG, while primer blaTEM has sequence F' ATCAGCAATAAACCAGC and R' CCCCGAAGAACGTTTTC. Data analysis was carried out by analyzing in silico results obtained from the NCBI website and observing the visualization results of PCR amplification indicated by the presence of DNA bands. In silico results showed 25 organisms attached to blaSHV and 29 organisms to blaTEM. In vitro results shown by electrophoresis visualization showed blaSHV amplicons measuring 392 bp with an annealing temperature of 50.5°C, and blaTEM measuring 517 bp with an annealing temperature of 42°C. The success rate of PCR was shown by clear and specific amplification of both genes, indicating that the PCR method performed was effective in detecting blaSHV and blaTEM genes in ESBL antibiotic-resistant bacterial isolate samples.
EVALUATION OF THE CAPABILITY OF IMMOBILIZED CHLORELLA VULGARIS (BEIJERINCK, 1890) IN ALGINATE BEAD MATRIX AS A BIOADSORBENT FOR HEAVY METAL ZINC (Zn)
No full textBioremediation intends to use living organisms to remove or decompose pollutants, using their ability to metabolize or accumulate hazardous substances, thus reducing toxicity. This study aims to compare the removal efficiency of free suspended Chlorella vulgaris and immobilized Chlorella vulgaris. Monoculture isolates of Chlorella vulgaris were cultured in an aqueous medium with KW21 fertilizer for approximately 12 days until reaching the stationary phase. The biomass was harvested, dried, and separated. A ZnCl₂ solution was prepared with 2, 4, and 8 ppm working concentrations. The biosorption efficiency was calculated to evaluate the biosorption capacity. The results indicated that the removal efficiency of free suspended C. vulgaris was 14.73%, 6.76%, and 10.22% for the respective concentrations. Immobilized C. vulgaris exhibited significantly higher removal efficiencies of 73.79%, 77.87%, and 83.80%. This study shows that immobilizeed C. vulgaris have a higher removal efficiency value than free suspended C. vulgaris cells because they can withstand heavy metal toxicity better. This study implicates that the treatment of alginate beads increases the stability and effectiveness of biosorption, and makes this method a preferred approach for heavy metal bioremediation applications in aquatic environments
MOSSES DIVERSITY IN GROJOGAN SEWU, JUMOG, AND PARANG IJO WATERFALL, KARANGANYAR DISTRICT
Get PDFMoss is a plant that can be found in low and highlands and lives attached to the substrate. The purpose of this study was to determine the diversity of lichens that grow in waterfalls located in Karanganyar district. The method used in this research was purposive sampling method. Based on the observations of the species found came from 5 classes namely Bryopsida, Marchantiopsida, Anthecerotopsida, Hepaticopsida and Jungermanniopsida. The level of diversity in each waterfall is influenced by environmental factors such as temperature, humidity and sunlight. Jumog and Parang ijo waterfalls have a high diversity index, while the evenness value of the three waters is high, and has the same dominance value which is classified as low
THERAPEUTIC EFFECTS OF Myrmecodia sp. ON THE MORPHOLOGY OF FALLOPIAN TUBE EPITHELIAL CELLS IN MICE WITHPELVIC INFLAMMATORY DISEASE
Get PDFPelvic Inflammatory Disease (PID) is a serious reproductive health issue affecting women of reproductive age, with Candida albicans as a common causative agent. This infection can damage the fallopian tube mucosa, increasing the risks of infertility, ectopic pregnancy, and chronic pelvic pain. With rising antimicrobial resistance, alternative therapies are needed. Myrmecodia sp. (ant plant) is a promising herbal candidate, rich in bioactive compounds such as flavonoids, tannins, terpenoids, and saponins, known for their antimicrobial, anti-inflammatory, and antioxidant properties. This study investigates the therapeutic effects of Myrmecodia sp. extract on the fallopian tube mucosa of BALB/c mice infected with Candida albicans. Using a true experimental post-test design, 24 mice were infected for four days and treated with varying concentrations of Myrmecodia sp. extract for seven days. Data were analyzed using ANOVA for vaginal color changes and the Kruskal-Wallis test for irritation. The results revealed that Myrmecodia sp. extract significantly reduced inflammation (p < 0.001) and showed a trend toward reduced irritation (p = 0.077). In conclusion, Myrmecodia sp. is a potential herbal therapy for PID caused by Candida albicans, offering a safe and effective option to support tissue regeneration and reduce complications
POTENTIAL FORMATION OF ACID MINE DRAINAGE BASED ON THE GEOCHEMISTRY OF GOLD ORE ROCKS
No full textOpen pit mining activities can have many impacts on the environment, such as the formation of mine pits containing acid mine drainage. This requires proper and correct handling so as not to harm the environment. The acidity of mine drainage can vary greatly depending on the quantity and type of minerals it contains. The objectives of this research are: to identify and determine the rock materials that have the potential to generate acid mine drainage (AMD) as Potentially Acid Forming (PAF) rocks and Non-Acid Forming (NAF) rocks. The research began with sample preparation, mineralogical analysis, AAS, static testing, and XRF testing. The research results indicate that the samples are primarily composed of quartz, with some hematite and goethite grains observed as fine, curved pore fillings. Gold (Au) is found within the mineral electrum (AuAg). The minerals present in the gold ore rocks are predominantly oxides, consisting of 84.7% SiO2, 8.23% Fe2O3, and other components. The rock samples are classified as NAF, as indicated by the paste pH analysis results of 7.05 and 6.94, total sulfur analysis values of 0.37% and 6.43%, and NAPP values of -5.1 and -3.5