BIOLINK : Jurnal Biologi Lingkungan, Industri dan Kesehatan
Not a member yet
260 research outputs found
Sort by
UJI EFEKTIVITAS DAUN PANDAN WANGI (PANDANUS AMARYLLIFOLIUS ROXB.) SEBAGAI ANTIBAKTERI TERHADAP SALMONELLA TYPHI
Get PDFPandanus amaryllifolius roxb leaves commonly used as food dditives which contain various compound groups namely alkaloid, saponins, flavonoids and tannins that have a role in antibacterial activity. This research is indicated to support the success of antibacterial in fragrant pandanus leaf plants against Salmonella typhi, one of the pathogenic bacteria. The antibacterial effectiveness test of ethanol-ethyl acetate extract (1: 1) of pandanus leaves which evaluated by the Kirby-bauer method and making the extract concentrations of 25%, 50%, 75%, 100%. The results showed that pandanus leaves extract had antibacterial effectiveness against Salmonella typhi with an average inhibition zone diameter of 11.6 mm; 14 mm; 14.3 mm; 15.3 mm; positive controls (Ciprofloxacin) 27 mm; and negative controls (aquadest) didn’t produce the inhibition zone of Salmonella typhi
ISOLASI DAN KARAKTERISASI BAKTERI RESISTEN TEMBAGA DARI PANTAI TIMUR SURABAYA
Get PDFCopper pollution in the East Coast of Surabaya is one of the serious cases of heavy metal pollution in Indonesia. Pollution at this location has been proven to result in fish deaths and brain damage of local residents because they consume too much copper-contaminated fish. Copper bioremediation using indigen bacteria isolated from polluted environments is a promising solution to overcome copper pollution problems. Bacteria that commonly live in polluted environments can be isolated and used as copper bioremediation agents. This study aims to do: 1) isolation and characterization of copper resistant bacteria from the East Coast of Surabaya, 2) resistance testing of bacterial isolates, and 3) copper accumulation and biosorbtion tests of bacterial isolates. The bacterial isolates were characterized by the morphology of the colonies and their cells based on Gram staining. Resistance testing is done by determining Minimum Inhibitory Concentration/MIC. The accumulation test is carried out by separating the cell and growth medium, then each of them is distructed using HNO3. The results of isolation and characterization obtained six isolates of copper-resistant bacteria, namely PmbC1, PmbC2, PmbC3, PmbC4, PmbC5, and PmbC6 with MIC = 3 mM - 5mM CuSO4. PmbC4 isolate is the most resistant bacteria with the MIC of 5 mM and is capable of accumulating copper of 6.25 mg per gram of cell dry weight and biosorbtion of 92.17%
RADIASI PLASMA PIJAR KORONA TERHADAP PERTUMBUHAN DAN KANDUNGAN NITROGEN TOTAL BAWANG MERAH DAN BAWANG BOMBAY
Get PDFShallots and onions are one of the leading vegetable commodities consumed by the public. The supply of these two onion varieties in Indonesia is not available at all times because is done seasonally. Therefore it is necessary to increase the cultivation, one of which is through the addition of nitrogen through plasma technology. This study to determine the effect of giving plasma nitrogen through plasma technology to the growth of bima onion brebes and grano onions. The study used a Completely Randomized Design (CRD) consisting of 6 treatments with 5 replications. Plasma radiation is carried out on onion seeds and onions with a radiation interval of 15 and 30 minutes. The growth parameters observed were shoot growth time, growth percentage, root length, number of roots, plant height, number of shoots, and nitrogen content. Data were analyzed using ANOVA followed by DMRT Test at 95% confidence level. The results showed that the onion irradiated by plasma for 15 minutes could increase growth and total nitrogen content, while in onions, the highest yield was precisely in the control treatment (0 minute radiation). In onions, plasma administration can inhibit growth and total nitrogen content 0,31%, but can increase the number of roots
PENGGUNAAN TANAMAN AREN PADA UPACARA SIPAHA LIMA MASYARAKAT PARMALIM
Get PDFThe purpose of this research are to know the beneficiaries and symbols contained in sugar palm plants in traditional ceremony Sipaha Lima. The method used in this research are the method of observation and depth interview. The data obtained in the form of plant parts used, how to obtain it and the meaning of symbol of the plant.The results is obtained eight types of use of sugar palm in traditional ceremony Sipaha Lima. Part of the plants used are: stems, leaves, and midrib. These plants are obtained from forests and crops
ISOLASI DAN AKTIVITAS ANTI MIKROBA BAKTERI ASAM LAKTAT DARI FERMENTASI NIRA KELAPA SAWIT
Get PDFThe aim of the study was to isolate lactic acid bacteria (LAB) from palm oil sap and to investigate the anti microbial activity of LAB against pathogen bacteria, Staphylococcus aureus and Escherichia coli using disc diffusion method. Palm oil sap were enriched using spesific medium MRS (Man Rogosa Sharpe) broth overnight, serial dilution were conducted and the culture were spread on the surface of MRS agar and then incubated for 48 hours. The result showed the total colonies of LAB were 1,4 x 107 CFU/mL. 6 colonies were randomly selected namely EHN1, EHN2, EHN3, EHN4, EHN5 and EHN6. The selected colonies are classified as Gram positive and bacilli in morphology. Antimicrobial activity against pathogenic bacteria was investigated using disc diffusion method. Antimicrobial activity of isolates against E.coli and S.aureus were 12,2 mm; 9mm; 11mm; 11mm; 10,5mm; 12 mm dan 13,5mm; 11mm; 12mm; 11,5mm; 12mm; 12,5mm respectively. from these result it can be concluded that LAB isolated from oil palm sap has potential as probiotic
Morfologi Spermatozoa Itik Lokal (Anas platyrhyncos) Pasca Preservasi Dalam Medium Berbeda Dikombinasi Krioprotektan Kuning Telur
Get PDFThe aim of this study was to assess the percentage of local duck spermatozoa morphology (Anas platyrhynchos) for 6 days of storage in three storage mediums combining various egg yolk (EY) concentrations.This experimental study applied three combinations of factors as treatment. Medium (Tris, PBS or Ringer lactate), egg yolk (EY) concentration 0,5,10,15 or 25% and storage time (0,1,2,3,4,5,6 day). Spermatozoa obtained by sequence method, ejaculate or sperm obtained in swim up in medium, swim up spermatozoa were used for treatment in storage medium according to treatment and assessed before and after morphology of storage in refrigerator temperature (50 C). The results showed that spermatoza morphology was significantly different (P <0.01) between 15 treatments. The medium factor and concentration of EY significantly (P <0.05) affected the morphology of treatment spermatozoa. The highest percentage of highest post-preservation spermatozoa morphology for six days was obtained in 5% yolk treatment in PBS medium (5EYPBS). This study proves that egg yolk cryoprotectants are good for maintaining the morphology of local duck spermatozoa
POTENSI BAKTERI KITINOLITIK NR09 PADA BEBERAPA MEDIA PEMBAWA DALAM MENGHAMBAT PERTUMBUHAN JAMUR PATOGEN Sclerotium rolfsii dan Fusarium oxysporum PADA BENIH CABAI MERAH (Capsicum annuum L.)
Get PDFThis study was aims to determine the viability of chitinolytic bacterial isolates in some carrier media and to know the potential of chitinolytic bacterial isolates in various carrier media in controlling the disease of sprout caused by F. oxysporum and S. rolfsii on red pepper plant. The method used by measuring the viability and ability of bacteria in growing media and carrier media on MGMK media after storage of carrier media for 90 days. Bacteria NR09 has good viability on peat carrier media and palm oil bunch compost and has the potential to inhibit attack of pathogenic fungi S. rolfsii and F. oxysporum
Biotransformasi Tartrazin oleh Bakteri Usus Manusia
Get PDFThe aimed of this research was to investigated human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri), to investigated dose of tartrazine, and to investigated biotransformation of tartrazine by human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri). The method of this research was experiment with significant correlation to investigated biotransformation of tartrazine by human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri). This study is primarily focussed on made bacteria medium, made standart inoculums of bacteria, and evaluation for activity of human intestinal bacteria (Streptococcus faecalis, Eschericia coli, Salmonella Sp., and Vibrio fischeri). The results obtained were attributed to the human intestinal bacteria was used in this research was biotransformated tartrazine. From the results that had been increase dose of tartrazine correlation with increase biotransformation by human intestinal bacteria. Highest dose of tartrazine was 15 mg, and highest biotransformation by Vibrio fischeri, which rate of zone decolourisation diameter was 1,22 cm, and rate of zone decolourisation was 1,44 cm2. Statistically showed significantly in correlation dose of tartrazine with rate of zone decolourisation (0,001 < P < 0,05), but statistically showed non significantly in correlation bacteria with rate of zone decolourisation (0,05 < P < 0,268)
KAJIAN PEMANFAATAN TUMBUHAN OBAT PADA MASYARAKAT SUKU SIMALUNGUN DI KECAMATAN RAYA DESA RAYA BAYU DAN RAYA HULUAN KABUPATEN SIMALUNGUN
Get PDFPenelitian ini bertujuan untuk mengetahui jenis-jenis tumbuhan yang dimanfaatkan sebagai obat tradisional, cara meramu, cara pengobatan, dan khasiatnya pada masyarakat suku Simalungun di Desa Raya Bayu dan Raya Huluan. Metode yang dilakukan dalam pengambilan data adalah survei eksploratif dan metode Participatory Rural Appraisal, yaitu proses pengkajian yang berorientasi pada keterlibatan dan peran masyarakat secara aktif dalam penelitian (Martin, 1995). Variabel yang diamati meliputi jenis tumbuhan, pemanfaatan bagian tumbuhan, cara meramu, bagian yang digunakan, dan khasiatnya. Hasil penelitian menunjukkan bahwa Diperoleh 53 Jenis Tumbuhan Obat yang terdiri dari 25 Ordo dan 33 Famili dari informasi 2 battra, Diperoleh 43 jenis ramuan dari 34 jenis penyakit, Famili Zingiberaceae merupakan famili yang paling banyak digunakan sebagai obat tradisional yaitu 12,96%, Bagian organ tumbuhan yang digunakan sebagai bahan obat tradisional adalah bagian daun dengan persentase 52,08%, Sumber Tumbuhan obat paling adalah hasil budidaya dengan nilai 75,46%, Ramuan Obat yang paling banyak digunakan adalah Ramuan Obat lambung, Hipertensi, Diabetes, Asam Urat dan Lever
KUALITAS AIR SUNGAI DI DESA TANIPAH (GAMBUT PANTAI), KALIMANTAN SELATAN
Get PDFTanipah village is a coastal village in Kecamatan Aluh-Aluh, Kabupaten Banjar, Kalimantan Selatan and a tidal area of sea water. Tanipah villagers utilize river water for daily needs, so the quality of river water is a major concern. The results of this research are expected to be informations for the community about quality of river water in Tanipah. This research is a survey research and the analysis was conducted in-situ and ex-situ. The analysis was done by comparing the test result against the predefined quality standard. River water temperature in Tanipah ranges from 280C – 33,60C and has no taste and no smell. Turbidity of 54,8 NTU, indicating river water is not feasible for consumption. Dissolved oxygen is 5,9 mg/L and is in the mild contaminated category. The COD and BOD value respectively 17,03 mg/L and 6,70 mg/L, exceeds the limit of the class I water quality standard. The pH value is at 7,33, indicating that the pH is normal. The iron concentration is 1,71 mg/L, exceeds the maximum permissible level for drinking water quality. Other metals likes Mn, Pb, Cu and Cd, are within thresholds for the waters. River water in Tanipah village based on the value of dissolved oxygen, is in category of light contaminated. Thus, in general, river water in Tanipah village can only be used for class III water, as irrigation