BIOLINK : Jurnal Biologi Lingkungan, Industri dan Kesehatan
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DETEKSI BAKTERI GENUS VIBRIO SEBAGAI CAUSATIVE AGENT PADA IKAN LELE SANGKURIANG (CLARIAS GARIEPINUS VAR. SANGKURIANG) DI KOTA TASIKMALAYA
Sangkuriang catfish (Clarias gariepinus var Sangkuriang) is the main commodity that widely cultivated in Tasikmalaya City. However, farmers have difficulty because of the disease which causes death in fish. High mortality is suspected as a vibriosis disease by genus Vibrio because redness wounds on dead fish. The purpose of this study is to know the genus Vibrio which causes of disease in Sangkuriang catfish. 10 samples of fish were taken from Sangkuriang catfish pond culture in Kelurahan Kersanagara Tasikmalaya City which were potentially suspected of vibriosis disease. Isolation of bacteria were done on TCBS medium. Bacterial isolates were collected from fish lesion on the body surface, liver, and kidneys of catfish. Isolation were able to gained 21 isolates and then 5 isolates (VK1, VK5, VK7, VK17, and VK21) were selected based on colony morphology and Postulates Koch’s were tested. The results showed that the clinical symptoms of catfish infected by vibriosis were redness lesions/ulcers on the body surface, hemorrhagic, fluid inside stomach, and fin eroded with redness wound. Bacterial identification through biochemical test revealed the causative agent of catfish disease at brackish pond area were bacteria of the genus Vibrio (VK 1, VK 5, and VK 7), Vibrio vulnificus (VK 17 and VK 21)
PENGARUH KADAR ASAM SULFAT PADA HIDROLISIS TANDAN KOSONG KELAPA SAWIT (TKS) DAN WAKTU FERMENTASI TERHADAP KADAR BIOETANOL YANG DIHASILKAN
The effect of sulphuric acid fermentation of processed palm oil bunch hydrolysis and on ethanol and sugar production was conducted. The aim was to investigate the hydrolytic optimum required by Saccharomyces cerevisiae for ethanol and sugar production. About 12.5 g powdered palm oil bunch was used in this experiment. The powder was hydrolized several times using sulphuric acid in several concentration. Sugar, product of hydrolysis, was detected every one hour using spectrophotometer. The sugar was fermented by Saccharomyces cerevisiae and incubated for several days. Results showed that 2-4% sulphuric acid was oprimum concentration for 30 g/L sugar production. The highest ethanol production 4.94% occurred after two days fermentation
PEMANFAATAN TUMBUHAN OBAT DIABETES MELLITUS DI MASYARAKAT ETNIS SIMALUNGUN KABUPATEN SIMALUNGUN PROVINSI SUMATERA UTARA
Diabetes Mellitus is a health issue that attracts a lot of attention because of the prevalence rate that increases every year, especially in developing countries like in Indonesia. Diabetes Mellitus (DM) is a condition of decreasing the function of the pancreas to produce insulin or insulin receptors are not sensitive so that a metabolic disorder occurs where glucose is not converted into glycogen so that glucose can not enter the cells, resulting in increased blood glucose. Simalungun ethnic is one of the ethnic group in Simalungun Regency of North Sumatera Province where the society of Simalungun ethnic still utilize herbs as an alternative treatment like diabetes mellitus, so it is necessary to do research about the Utilization of Diabetes Mellitus Medicinal Plants In Simalungun Ethnic Society of Simalungun Regency of North Sumatera Province. This research was conducted using exploratory survey by the independent variable informant. Data collection was done by in-depth interview technique to informant. The results obtained 26 species of plants, consisting of, 20 families and 15 orders that have potential as an antidiabetic drug. Plant parts used are roots, leaves, fruit, stems, bark, seeds and tubers. And the most widely used is the leaves as much as 40.74%
ISOLASI DAN KARAKTERISASI BAKTERI ENDOFIT PENGHASIL ENZIM KATALASE DARI DAUN BUASBUAS (PREMNA PUBESCENS BLUME)
This research aims to determine the presence of endophytic bacteria producing catalase enzyme in Buasbuas (Premna pubescens Blume) leaves and how endophytic bacteria producing catalase enzym characteristics of the Buasbuas (Premna pubescens Blume) leaves. This research was conducted with several characterization stages: macroscopic characterization (morphology), microscopic characterization (gram staining) and catalase test. Data analysis technique used is descriptive. The isolation result there are 5 isolates of endophytic bacteria and 3 isolates which have potential to produce catalase enzyme
EFEKTIVITAS BEBERAPA PRODUK PEMBERSIH WAJAH ANTIACNE TERHADAP BAKTERI PENYEBAB JERAWAT Propionibacterium acnes
The purpose of this study was to determine the effectiveness of some antiacne facial cleanser products against bacteria that cause acne Propionibacterium acnes, which conducted experimentally using 6 product against of antiacne facial cleanser bacteria cause Propionibacterium acnes with three replication. The parameters observed were the inhibitory zone diameters. The results showed that of some antiacne facial cleanser products that are most effective in inhibiting the growth of acne-causing bacteria Propionibacterium acnes is the product CCA code, with a 35 mm inhibit zone, because CCA products were contain 6 combinations of natural ingredients from Hamamelis virginia, Melaleuca alternifolia, Cinnamomum zeylanicum, Cedrus atlantica, Portulaca oleracea and olive oil All antiacne products are shown to have effectiveness against the bacteria Propionibacterium acnes
KUALITAS SPERMATOZOA DAN INDEKS FERTILITAS TIKUS PUTIH DEWASA (Rattus novergicus L.) SETELAH PEMBERIAN EKSTRAK DAUN WUNGU (Graptophyllum pictum. L.Grifft)
This research objective is to identify the effects of wungu leaf extract (Graptophyllum pictum L. Griff) on the fertility index and spermatozoa of normal morphology in male rat ( Rattus novergicus.L). Fifteen male and 45 female rats of 9 to 10 weeks old were used as research samples. The fifteen male rats were divided into five treatment groups of wungu extract dosage: control I (0mg/KgBW), control II (0.5ml olive oil), dosage 1 (100mg/KgBW), dosage 2 (200mg/KgBW), and dosage 3 (400mg/KgBW) for 60 days. The extract was given orally and each group consisted of three test rats as repetition. On the day 55 to day 60, each test male animal was mated with three female rats. After 60 days, an observation was done to 15 male and 45 female rats to identify fertility index. Normal spermatozoa morphological observation with microscope. The data obtained were tested by Analysis of Variance Test (ANOVA ? = 0.05) and Duncan’s Multiple Range Test (DMRT). Based on the observation and statistical analysis, it was found that wungu leaf extract of different dosages did not give significant effects on fertility index but have significant effects on spermatozoa of normal morphology. This was proven by the fact that the higher the dosage given, the worse the spermatozoa of normal morphology. Based on Linear Regression Analysis, it was found significant correlation between dosage variation extract of wungu leaf and fertility index, and number of normal spermatozoa
Efek Ekstrak dan Fraksi Herbal Peperomia pellucida (L.) Kunth., Terhadap Beberapa Bakteri Patogen Kulit
The study of extract and fraction of Peperomia pellucida (L.) Kunth., herbal’s against several dermal pathogenic bacteria have been carried out in Research Laboratory of Pharmacy Department and Microbiology and Micology Laboratory Biology Department Faculty Mathematic and Natural Sciences Andalas University and Regional Medical Laboratory Center in Gunung Pangilun Padang from July until October 2007. Three kinds of dermal pathogenic bacteria were used : Staphylococcus aureus, Staphylococcus epidermidis and Proteus vulgaris. Antimicrobial activity was determined base on their MIC (Minimal Inhibitory Concentration) and MBC (Minimal Bactericidal Concentration) through dilution method that used “Microtiterplate 96-wellâ€. The Result showed that the best activity of herbal extract was against Proteus vulgaris (MIC 7,81 ppm and MBC 125 ppm). The most active fraction was showed in n-hexane against Proteus vulgaris (MIC 0,63 ppm and MBC 250 ppm), the etil acetat fraction (MIC 2,50 ppm and MBC 500 ppm), while the activity of n-butanol and aquaeous fraction (MIC 5 ppm and MBC 500 ppm, respectively)
FAKTOR – FAKTOR YANG MEMPENGARUHI PERMINTAAN TANAMAN ANGGREK (Orchidaceae) DI KOTA MEDAN
The purpose of this study was to determine the effect of prices, consumer tastes, income and location / place to Requests Orchid Plant in the city of Medan, and to know the Demand Elasticity of orchids in the city of Medan. The data collected from the data Primary and Secondary Data. Based on the results of testing statistical analysis obtained by value Multiple R of 86.6%. The value of F table at the level of 0.05 is thus calculated F 2,76 = 18.801> F table = 2.76 at 95% confidence level. Furthermore, in testing T test, to see the effect of demand factors partially on the request of orchids, the value of the T-table 2.045 with a confidence level of 86.6%. X2, X3 and X4 real impact While real variables X1 No Effect on Demand (Y). Analysing the value of Elasticity, Elastic Retrieved much as 3.395> 1. Demand level of sensitivity to the independent variable (tastes, income, and location) of 3.395
KEANEKARAGAMAN NEPENTHES DI KECAMATAN SIPIROK
This research aims to determine the diversity of Nepenthes species in Sipirok sub-district. Location determination research using survey method. The collection of the data is performed using the methods of exploration. Found 7 types of Nepenthes eustachya Nepenthes i.e., Nepenthes ovata, Nepenthes gracilis, Nepenthes rhombicaulis, Nepenthes reinwardtiana, Nepenthes tobaica and Nepenthes reinwardtiana natural silangan one x Nepenthes tobaica. Nepenthes found the lowlands and the Highlands. Nepenthes eustachya is found at an altitude of 1008 mdpl, where this type previously found only in lowland Sumatra
KETEPATAN PEMBERIAN ANTIBIOTIK PADA PASIEN ISPA BAGIAN ATAS DI PUSKESMAS PEKAN LABUHAN MEDAN PADA BULAN JANUARI – JUNI 2017
Acute respiratory tract infection is one of the diseases that attack the main chanel of the raspiration of the nose, alveoli, andeksana, sinus, middle ear cavity and pleura. Acute infection is an infection that lasts for a 14 day time limit. This study aims to determine the accuracy of antibiotics in patients with URI in public health center Pekan Labuhan Medan is in accordance or not in accordance with the basic treatment guidelines in public health center 2007. Administration of antibiotics based on diagnosis of upper respiratory infection patients at Public Health Center Pekan Labuhan Medan of 93,2% there is not yet according to basic treatment guidance in Public Health Center 2007 which is set. Such discrepancies include the type of antibiotics and the suitability of the indication of administration