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Biosorption of fluoride from aqueous solutions by Rhizopus oryzae: Isotherm and kinetic evaluation
No full textRemoval of high fluoride contents from aqueous solutions by inexpensive, and biocompatible biosorbent is a main concern for human health. In the present study the efficacy of Rhizopus oryzae fungal biomass in removal of fluoride from aqueous solutions considering the effects of different parameters such as contact time (10–180 min), initial fluoride concentration (2–10 mg/L), and biosorbent dose (0.25–2 g/L) was studied in a batch system. The optimum condition for the maximum fluoride biosorption (90.5%) was obtained at 2 g/L biosorbent dose, 30 min contact time and 2 mg/L initial fluoride concentration. The Langmuir model (R2 = 0.9844) fitted better than the other models and showed a homogeneous biosorption surface with the possibility of a monolayer biosorption of fluoride by biosorbent. The Langmuir maximum biosorption capacity of fluoride for R. oryzae was obtained at 1.16 mg/g. The biosorption kinetic was controlled by the pseudo-second-order (R2 = 0.9999) model and based on the intra-particle diffusion model; the fluoride biosorption rate was not controlled only by the mechanisms of intra-particle diffusion. Consequently, the results showed that fungal biomass can be used as a suitable biosorbent for fluoride removal from aqueous solutions. © 2021 American Institute of Chemical Engineers
Azole antifungal resistance in candida albicans and candida glabrata isolated from vulvovaginal candidiasis patients
Get PDFBackground: Vulvovaginal candidiasis (VVC) is the most frequent fungal disorder in healthy and normal women. Objectives: The aim of this study was to evaluate the in vitro antifungal susceptibility of clinical isolates Candida albicans and Candida glabrata, the two most common candida species in Iranian patients with VVC. Methods: One hundred and eight clinical isolates of candida, including; C. albicans (n = 77) and C. glabrata: (n = 31) were isolated from the 108 patients with VVC. The in vitro activity of caspofungin (CAS), amphotericin B (AMB), voriconazole (VRC), itraconazole (ITC), fluconazole (FLC), and nystatin (NYS) were determined according to the CLSI M27-A3 and CLSI M27-S4. Results: Our results were shown 8 (25.8 %) and 6 (7.8 %) C. glabrata and C. albicans isolates resistance to FLU, respectively. Furthermore, resistance to VRC and ITC were observed in 8.4%, and 3.7% of all isolates, and six isolates (5.6%) had intermediate MIC to CAS. Conclusions: We reported 8 (25.8 %) and 6 (7.8 %) C. glabrata and C. albicans isolates resistance to FLU, respectively. Furthermore, resistance to VRC and ITC were observed in 8.4% and 3.7% of all isolates, respectively. © 2021, Author(s)
Modulation of LXR signaling altered the dynamic activity of human colon adenocarcinoma cancer stem cells in vitro
Get PDFBackground: The expansion and metastasis of colorectal cancers are closely associated with the dynamic growth of cancer stem cells (CSCs). This study aimed to explore the possible effect of LXR (a regulator of glycolysis and lipid hemostasis) in the tumorgenicity of human colorectal CD133 cells. Methods: Human HT-29 CD133+ cells were enriched by MACS and incubated with LXR agonist (T0901317) and antagonist (SR9243) for 72 h. Cell survival was evaluated using MTT assay and flow cytometric analysis of Annexin-V. The proliferation rate was measured by monitoring Ki-67 positive cells using IF imaging. The modulation of LXR was studied by monitoring the activity of all factors related to ABC transporters using real-time PCR assay and western blotting. Protein levels of metabolic enzymes such as PFKFB3, GSK3β, FASN, and SCD were also investigated upon treatment of CSCs with LXR modulators. The migration of CSCs was monitored after being exposed to LXR agonist using scratch and Transwell insert assays. The efflux capacity was measured using hypo-osmotic conditions. The intracellular content of reactive oxygen species was studied by DCFH-DA staining. Results: Data showed incubation of CSCs with T0901317 and SR9243 reduced the viability of CD133 cells in a dose-dependent manner compared to the control group. The activation of LXR up-regulated the expression and protein levels of ABC transporters (ABCA1, ABCG5, and ABCG8) compared to the non-treated cells (p < 0.05). Despite these effects, LXR activation suppressed the proliferation, clonogenicity, and migration of CD133 cells, and increased hypo-osmotic fragility (p < 0.05). We also showed that SR9243 inhibited the proliferation and clonogenicity of CD133 cells through down-regulating metabolic enzymes PFKFB3, GSK3β, FASN, and SCD as compared with the control cells (p < 0.05). Intracellular ROS levels were increased after the inhibition of LXR by SR9243 (p < 0.05). Calling attention, both T0901317 and SR9243 compounds induced apoptotic changes in cancer stem cells (p < 0.05). Conclusions: The regulation of LXR activity can be considered as a selective targeting of survival, metabolism, and migration in CSCs to control the tumorigenesis and metastasis in patients with advanced colorectal cancers
Three-dimensional and two-dimensional relationships of gangliogenesis with folliculogenesis in mature mouse ovary: a Golgi–Cox staining approach
Get PDFThe present study was set out to investigate two-dimensional (2D) and three-dimensional (3D) evaluations of ovarian nervous network development and the structural relationship between folliculogenesis and gangliogenesis in mouse ovaries. Adult mice ovarian tissue samples were collected from follicular and luteal phases after cardiac perfusion. Ovarian samples were stained by a Golgi–Cox protocol. Following staining, tissues were serially sectioned for imaging. Neural filaments and ganglia were present in the ovaries. In both 2D and 3D studies, an increase in the number and area of ganglia was seen during the follicular growth. The same pattern was also seen in corpora lutea development. However, in some cases such as ratio of ganglia number to follicle area, the ratio of ganglia area to follicular area, 2D findings were different compared with the 3D results. 3D analysis of ovarian gangliogenesis showed the possible direct effect of them on folliculogenesis. Golgi–Cox staining was used in this study for 3D evaluation in non-brain tissue. The results of 3D analysis of the present study showed that, in some cases, the information provided by 2D analysis does not match the reality of ovarian neuronal function. This confirmed the importance of 3D analysis for evaluation of ovarian functio
Prevalence of depression in iranian college students: A systematic review and meta-analysis
No full textContext: Depression is a common problem in students. The current study aimed to determine the prevalence of depression in Iranian college students. Evidence Acquisition: The study was conducted using preferred reporting items for systematic reviews and meta-analysis (PRISMA). We searched both international (i.e. PubMed, Scopus, Science Direct, Web of Science, Embase, and Google Scholar) and national (i.e. SID, Magiran, Iranmedex, and IranDoc) databases from 1991 to June 2019 to identify relevant studies. Cochran’s Q test, I2 index, and τ2 index were used to assess the heterogeneity. A random-effects model was employed to combine the results using comparative meta-analysis software version 2.2. Results: Overall, 89 studies dealing with a total of 33,564 Iranian students were included. The prevalence of depression was estimated at 48.0% [95% confidence interval (CI): 43.6 - 52.5%]. The prevalence of depression was 51.3% (95% CI: 41.1 - 61.4%) and 48.9% (95% CI: 41.0 - 54.8%) in males and females, respectively. The prevalence of mild, moderate, and severe depression was estimated at 26.1% (95% CI: 23.8 - 28.6%), 15.5% (95% CI: 13.1 - 18.3%), and 6.4% (95% CI: 5.1 - 8%), respectively. The Beck-I Questionnaire and the GHQ-28 Questionnaire indicated the highest and the lowest prevalence of depression [68.4% (95% CI: 38.8 - 88.1%) vs. 39.2% (95% CI: 24.6 - 56.1%)], respectively. It was proved that the prevalence of depression among Iranian college students has significantly increased during the study period (P < 0.001). Interest in the field was significantly correlated with depression (P < 0.001). Conclusions: Given the high prevalence of depression among Iranian college students, prevention and treatment programs are strongly recommended
An innovative index for assessing vulnerability of employees of different occupations from the COVID-19 pandemic in Iran
No full textThe vulnerability of employees of different occupations from the Coronavirus disease 2019 (COVID-19) pandemic in Iran was assessed using an innovative index. The vulnerability index was developed in five steps as follows: (1) determining the principles and components of employees' susceptibility and resilience, (2) weighting the principles and components, (3) converting the levels of components to the sub-index values, (4) introducing the aggregation functions, and (5) characterizing the vulnerability index values in five categories as very high (80–100), high (65–79), medium (50–64), low (30–49), and very low (0–29). The average values of susceptibility, resilience, and vulnerability index of the employees were determined to be 35.2 ± 15.0, 73.9 ± 17.0, and 32.9 ± 12.7, respectively. The average resilience of the employees was more desirable than their average susceptibility. The distribution of the employees into the vulnerability index categories was 46.3% for very low, 41.9% for low, 3.6% for medium, and 8.2% for high. The worst cases of susceptibility and resilience principles were exposure to contaminated surfaces (59.1 ± 22.8) and top management commitment (66.6 ± 23.1). The elderly staff (especially over 50 years old), employees with low education levels, and employees in private and self-employment sectors were significantly more vulnerable (p value < 0.01) from the COVID-19 pandemic. The principles with significant incremental effects on the vulnerability index (p value < 0.05) were respectively top management commitment (+1.78), exposure to COVID-19 patients at work (+1.36), exposure to contaminated surfaces (+0.82), installing clear shields and wearing PPE (+0.59), observance of social distancing (+0.48), and just culture (+0.22). An especial plan to support the more vulnerable employees with an emphasis on the principles with the most incremental effects on the vulnerability index can efficiently control the inequality between the employees as well as occupational transmission of the COVID-19 in Iran
Characterization, possible sources and health risk assessment of PM2.5-bound Heavy Metals in the most industrial city of Iran
Get PDFAir pollution associated with particulate matters results in different types of disease including allergy, lung destruction, heart failure, and related problems. This study has been designed and performed to examine the concentration of PM2.5-bound heavy metals, risk assessment, possible sources and effect of meteorological parameters on 17 sites of the air of the most industrial city of Iran (Karaj) in 2018-19. For this purpose, four samples were taken from every point of Karaj air over one year using a pump (Leland Legacy (SKC)) with flow rate of 3 L/min on PTFE filter for 24 h. Overall, 68 samples of PM2.5-bound heavy metals were collected. Note that during the sampling, atmospheric parameters including temperature, pressure, humidity, and wind speed were regularly recorded using PHB318 portable device. In examining the chemical composition of these particles, the concentration of metals (Al-Zn- Ar-Cd-Cr-Cu-Fe-Hg-Mn-Ni-Pb) was determined after digestion of the collected samples and through injection into ICP-OEC device. The results indicated that the mean annual concentration of PM2.5 particles range from 21.84 to 72.75 µg/m3. The mean concentration of heavy metals lied within the range of 25.63 to 336.27 ng/m3. Among heavy metals, the maximum concentration belonged to aluminum (277.95 ng/m3) and iron (336.27 ng/m3), which are known as elements with a ground source (sources such as car fuels, exhaust gases, decorative materials, batteries, indoor smoking, the paint used for painting walls, erosion and corrosion of rubber of cars). Meanwhile, there was a positive relationship between heavy metals and temperature(r: 0.418, p < 0.019), pressure (r: 0.184, p < 0.0.402), as well as wind speed (r: 0.38, p < 0.017), while an inverse relationship was observed with relative humidity (r: -0.219, p < 0.018). The ecological risk of the metals calculated was very notable, with the maximum environmental risk being related to cadmium in children (6.61) and manganese in adults (0.82). The largest HQ in children and adults was associated with Cr. Finally, ILCR values for cadmium in both children (1.19 E-04) and adult (4.81 E-04) groups indicated high risk of developing cancer in humans
Intraprostatic prophylactic antibiotic injection in patients undergoing transrectal ultrasonography-guided prostate biopsy
No full textObjectives: To evaluate the efficacy of intraprostatic injection of prophylactic antibiotics for the prevention of infections following transrectal ultrasonography-guided prostate biopsy. Methods: In this prospective interventional study, patients aged between 40 and 70 years with clinical indications for transrectal ultrasonography-guided prostate biopsy were enrolled. Consecutive patients who received intraprostatic injection of amikacin coupled with oral ciprofloxacin were compared with historical controls receiving only prophylactic oral fluoroquinolones prior to biopsy. Patients were followed for 7 days after biopsy for signs and symptoms of infection. Results: A total of 210 patients were included in this study (mean age 65.7 ± 7.8 years). Among patients without intraprostatic injection, nine (8.6%) developed infectious complications (lower urinary tract infection in seven patients, and prostatitis in two), which led to hospitalization in six patients (5.7%). None of the patients who received intraprostatic antibiotic injection developed infectious complications. The difference in the incidence of post-transrectal ultrasonography-guided biopsy infections between the two groups was statistically significant (P = 0.003). Conclusion: Local intraprostatic antibiotic injection during transrectal ultrasonography-guided prostate biopsy adjuvant to oral fluoroquinolones can significantly reduce the rate of post-biopsy infectious complications
Cardiotoxicity and cardiac monitoring following the use of radiotheranostics agents including 177Lu-PSMA for prostate cancer and 177Lu-DOTATATE for neuroendocrine tumors
No full textBackground The aim of this study was to determine the probable cardiotoxicity following radionuclide therapy (RNT), specifically peptide receptor radionuclide therapy (PRRT) with 177Lu-DOTATATE and radioligand therapy (RLT) with 177Lu-PSMA by evaluation of serum troponin I and cardiac profile change during a follow-up time. Materials and Methods Patients with prostate cancer and neuroendocrine tumours (NETs) referred for PRRT and RLT, respectively, were enrolled in this study. The cardiac profiles of the patients were evaluated by a cardiologist and a cardiac history was obtained from all patients. Also, troponin I was measured before and 48 hours after treatment. Results In this retrospective study for assessment of RLT associated cardiotoxicity, 24 patients were evaluated with a median age of 64 years (27-99 years) including 13 NET patients and 11 prostate cancer patients. Patients were followed up for 4 to 31 months which no cardiovascular problem was observed. In evaluation of troponin I, 39 RNT cycles were evaluated. In all patients, the value of troponin I was in normal range. In all patients, the median values of serum troponin I before and after treatment were 0.2±0.02 (range: 0.00-0.42) and 0.28±0.02 (range: 0.00-0.46) ng/ml, respectively (p>0.05). In the prostate cancer patients, the median values of serum troponin I before and after treatment were 0.26± 0.04 (0.04-0.42) and 0.30±0.04 (0.00-0.41) ng/ml, respectively (p>0.05). In the NET patients, the median values of serum troponin I before and after treatment were 0.18± 0.03 (0.00-0.42) and 0.17±0.03 (0.00-0.46) ng/ml, respectively (p>0.05). Conclusion PRRT with 177Lu-DOTATATE and RLT with 177Lu-PSMA as emerging therapeutic modalities have no significant cardiotoxicity. However, further well-designed studies are recommended
COVID-19 and smoking: More severity and death-An experience from Iran
No full textBackground: Few studies have shown that smokers are more likely than nonsmokers to contract COVID-19, while others report that smokers are underrepresented among those requiring hospital treatment for this illness. This study was designed and implemented for investigating the severity and outcome of COVID-19 based on underlying smoking status. Materials and Methods: This was a case-control study that was implemented in Tehran and Ahvaz with all COVID-19 patients from February to July 2020. Patients were divided into two groups of COVID-19 positive (1044 cases) and negative (1231 controls) randomly based on entrance number. Results: The frequency of smokers in the case group is significantly lower than the control group (15.4% vs. 28%). However, the number of days of hospitalization and the use of more than 3 medications in the group of smokers were significantly higher. A comparison between groups of case and control based on smoking indicates that the death rate was significantly higher in smokers with COVID-19. Conclusions: The results of this study showed that although the number of smokers in COVID-19 patients is significantly lower, smokers run an aggresive curse and have higher mortality