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Objective Structured Clinical Examination (OSCE)
No full textThroughout your nursing degree you will need to undertake a broad range of assessments, each requiring a different skill set in order to pass. Taking an assessment literacy approach that explains the purpose and process behind each assessment, this book offers practical tips and guidance to help you feel confident, demonstrate learning and application to practice and achieve success
Novltex: a new class of antibiotics with potent activity against multidrug-resistant bacterial pathogens - design, synthesis, and biological evaluation
Get PDFIncreasing spread of multidrug-resistant (MDR) bacteria demands antibiotics that combine potent activity with scalable synthesis. Novo29 (clovibactin) is promising but suffers from low yield (1%), dependence on costly and noncommercial d-hydroxy-asparagine (d-Hyn5), and lengthy syntheses. We report “Novltex”, a novel class of antibiotic that fuses the Leu10-teixobactin macrocycle to the Novo29 N-terminus tail, replacing d-Hyn5 with inexpensive threonine. Our efficient synthesis delivers 30% yield with faster coupling cycles (∼10 min), enabling rapid and low-cost scale-up. A 16-member analogue library systematically probing amino-acid configuration identified analogue 4 (d-Leu2) as the initial lead, informing the rational design of analogue 12 (d-cyclohexylalanine2). Analogue 12 displays potent antibacterial activity (minimum inhibitory concentration (MIC) 0.12–0.5 μg/mL) against World Health Organization (WHO)-priority pathogens, including methicillin-resistant Staphylococcus aureus (MRSA) and Enterococcus faecium, surpassing several licensed antibiotics while maintaining an excellent safety profile. Lipid II-binding assays confirm the conservation of the parent mechanism. Novltex, therefore, offers a practical, high-yielding, and cost-efficient platform for the development of next-generation antibiotics targeting MDR infections
Who Cares installation at IFTR 2025
No full textWho Cares is a video installation work created by choreographer Angela Woodhouse and visual artist Caroline Broadhead using dance and thermal imagery that draws on the expanded body through heat data to explore themes of touch, heat trace, intimacy, and care.
Dancers Martina Conti and Alice Labant.
The piece is made up of 7 videos between 3-5 minutes in length. You are free to view for as long as you wish.
Videos in order individually titled:
Distancing
Mingle
Stir
Cover up
In Isolation
Shift
Trac
Not knowing, yet living: AI and the modern legal trial of Prometheus in medicine
No full textThis paper explores the paradox of Artificial Intelligence (AI), particularly Machine Learning (ML), in modern medicine, promising enhanced diagnostics and treatment while in many instances also leading to a profound loss of transparency and interpretability. Complex AI systems such as Automated Decision-Making Systems (ADMS) increasingly guide critical clinical decisions; however, their opaque “black box” nature challenges traditional medical ethics centred on transparency, due process, accountability, and patient autonomy. ADMs often make recommendations that cannot be fully understood or explained due to the system’s opacity. This raises both legal and ethical concerns. For example, the ability of clinicians to justify clinical decisions informed by AI when they themselves lack insight into its reasoning raises concerns about clinical legal liability. This, in turn, raises ethical concerns regarding patient autonomy if patients are unable to meaningfully make informed choices on treatments due to the opacity of decisions made by AI. The extent to which patients should consent to and place their trust in life-saving technologies whose inner workings remain opaque remains an open debate. These technologies, arguably, can diminish patients’ rights to knowledge, autonomy, and dignity. Such concerns underscore the fragility of trust within clinical relationships and expose the limitations of prevailing frameworks for medical explanation (MedX) and informed consent. This paper examines the role of explainability in AI-driven medicine, arguing that while explanation is essential, it must be balanced against the clinical utility of high-performing, opaque systems. To address this tension, the paper proposes the Promethean Threshold Heuristic (PTH)—a normative framework designed to reconcile the need for explanation with clinical efficacy. Drawing on Promethean myth, it frames the ethical dilemma posed by AI as one of balancing risk, knowledge, and human dignity. Ultimately, it calls for principled AI governance in healthcare—one that preserves trust, prioritises transparency where feasible, and recognises the trade-offs inherent in deploying powerful but opaque technologies
Integrated cellular, proteomic and metabolite analysis of argyrin B with rational design approaches for immunoproteasome-selective inhibitors
No full textThe ubiquitin-proteasome system plays a critical role in cellular protein degradation and homeostasis. Current proteasome inhibitors (PI) present great efficacy against haematologic cancers due to the high secretory load creating an increased dependency on proteostasis, yet resistance and adverse effects persist. The immunoproteasome has recently emerged as an attractive and selective target due to its preferential expression in disease states. Here the constitutive proteasome (CP) active sites β1c, β2c and β5c are replaced with immunoproteasome isoform containing β1i, β2i and β5i. Many ongoing efforts are focused on studying immunoproteasome-selective inhibitors, characterising their active site binding specificity and biochemical impact.
Argyrin analogues are naturally derived cyclic peptides showcasing antibacterial, immunosuppressive and anti-tumour effects. Efficacy and mechanisms vary by analogues, many of which are not characterised nor assessed in a variety of models. Despite this, there is demonstrable therapeutic potential through proposed targeting of the proteasome and mitochondrial elongation factor G; both integral to proteostasis. Further to this, argyrin B analogue has shown potential for selective inhibition of the β1i site of the immunoproteasome. This thesis extents studies on argyrin analogues as potential proteasome inhibitors through in silico analysis. Alongside biological studies of argyrin B cellular impact, proteomic effect and phase I metabolism.
Potential interactions of 16 argyrin analogues at CP and immunoproteasome active sites were studied by molecular docking using Autodock 4.2. Results showed striking preferential binding at β1i site over β1c site (p<0.05) in 75% of analogues, with analogues K, J, I and B additionally exhibiting improved β5i over β5c binding (p<0.05). For further SAR insights, a panel of naphthyl-azotricyclic-urea-phenyl scaffold-based compounds were docked using the same methods, corroborated through in vitro purified enzymatic assays. The key reasons for observed selectivity of these compounds are differences in subunit protein sequences, including substitutions G97H and M95S in β1c-β1i and T21S, A46S and S116E β5c-β5i, alongside differences in hydrophobic motifs that may have broader applications in rational drug design.
Argyrin B was further characterised for in vitro cellular impact in RPMI8226 multiple myeloma (MM) cells and noncancerous B-lymphocyte derived RPMI1788 cells and compared with the established PI, carfilzomib. From MTT assays and flow cytometry analysis, argyrin B exposure revealed cytotoxicity at low micromolar IC50 values. Interestingly, an increased sensitivity was shown towards RPMI8226 compared to RPMI1788, in contrast to carfilzomib. Quantitative proteomics experiments at IC50 doses, using UHPLC coupled to a Q-exactive Orbitrap mass spectrometer were employed to study the effects of argyrin B and carfilzomib on protein expression. Indicative of PIs, both compounds and cells triggered an unfolded protein response with upregulation of heat shock proteins, autophagy and proteasome bounce-back. Distinct regulation and putative selective MM targets identified in RPMI8226 included: STHM1, API5, PARK7 and MIF. Compared to carfilzomib, argyrin B exhibited greater differential protein expression between the cell types, yet overall at both cell types fewer biological pathways were impacted (FDR<0.005), together suggesting a more targeted action. Finally, the microsomal degradation of argyrin B followed by detection of metabolite compounds, exhibited oxidation and demethylation transformations, providing links back to SAR applications and analogue differences.
In summary, this study identified site-selective mechanisms for IP inhibition through in-silico investigation of argyrin analogues and analogues based on a naphthyl-azotricyclic-urea-phenyl scaffold. Further to this, the unique biochemical impacts of argyrin B on MM and B-lymphocyte cells were identified. With distinct impacts from carfilzomib, this can help to overcome current issues of resistance and off-target effects. Combined, the results from this study will advance the development of next generation PIs and understanding of the therapeutic applications of argyrins
Gestaltungsanforderungen an das trimediale Kommunikationskon-strukt von Geschäftsleitung, Pflegefachperson und Patient/in zur Förderung der Patientenzufriedenheit - Einzelfallstudie am Beispiel des Regionalspitals GZO AG Wetzikon (CH)
No full textThe Swiss healthcare system is currently facing numerous challenges. One of these is the continuous increase in health insurance premiums, which leads to rising costs. Additionally, the workload and working conditions of nursing staff are being criticized, resulting in many leaving the healthcare sector. The resulting shortage of qualified healthcare professionals impacts the quality of care. Hospital management must also address the consequences of healthcare policy reforms, which aim to reduce costs, improve transparency, and increase efficiency. Public discussions are heavily focused on costs and maintaining the quality of care.
From the management’s perspective, patients are the most important group, as they ensure the hospital’s existence. Nevertheless, nursing staff are increasingly coming into the focus of management because the growing demand for qualified nursing personnel is leading to intense competition within the industry, forcing hospitals to critically review and adjust their working conditions. Nursing staff play a crucial role in patient care as they are responsible not only for patient care but also for contributing to efficiency and, therefore, cost control, acting as carriers of organizational culture. Given this situation, it is essential for management to shift their focus to ensure the hospital’s competitiveness and success.
The theoretical section highlighted the relationship between communication and patient satisfaction as a central theme in the Swiss healthcare system, as it has a direct impact on perception and thus the experienced quality of care. This involves not only understanding factual content but also addressing emotional aspects that play a role during an illness. Direct patient communication not only enhances the understanding of specialized knowledge but also fosters trust and quality and, therefore, has a direct impact on customer retention. Categories were defined for each group to allow for further structured empirical work on the topic.
The study was designed as a single case study and conducted at the GZO AG regional hospital in Wetzikon, where various requirements for the management team, nursing staff, and patients were identified. It was noted that direct communication with patients can be considered a decisive success factor
Anti-war protest: the historic case of Novaya Gazeta and Others v Russia
No full textA discussion of the importance of the separate opinion of Judge Darian Pavli in the ECtHR judgment of Novaya Gazeta v Russia (2025
A digital twin framework with MobileNetV2 for damage detection in slab structures
Get PDFIn this study, a digital twin framework is proposed for damage detection in a civil structure, which consists of a finite element model, neural networks, model updating methods, and signal processing. To verify the proposed framework, we present a case study of slab structure using deflection measurement as input data. The dynamic characteristics of the physical model are used to calibrate the digital twin model. Damage scenarios are created on the digital twin model. The defection of the damaged slab under static loads is analyzed with two-dimensional discrete wavelet theory (DWT), whereas the diagonal wavelets are used to extract images data set used to train the convolutional neural network (CNN). MobileNetV2 uses transfer learning can reduce the number of trained parameters and hence perform fast convergence. The proposed method gives high accuracy about detection of low-severity damage having the severity less than 10%. There is more than 80% accuracy for predicting the damaged location and its severity. The success of using MobileNetV2 and transfer learning helps to improve the methods further on mobile devices and the potential for more applications. Moreover, the proposed framework does not require the data of the intact structures, leading to much wider applications
Measuring quality in the baby room: what the global evidence says
Get PDFA new research project is looking into the quality of provision in English baby rooms following the Government's expansion of subsidised education and care to infants as young as nine months. In the first of two articles, Dr Mona Sakr shares more about the project and how the quality of provision is defined and measured
Understanding and managing iron deficiency anaemia
Get PDFIron deficiency anaemia develops when there is not enough iron in the body to sustain normal red blood cell production. It is a major cause of morbidity worldwide and is linked to a range of comorbid conditions, including gastrointestinal cancer. In the UK, iron deficiency anaemia is the most common cause of anaemia identified in primary care and is estimated to affect 3% of men and 8% of women. Nurses can support the management of iron deficiency anaemia by identifying possible causes, ordering blood tests, advising on diet and iron supplementation, and referring patients for additional investigations as required. This article provides an overview of the pathophysiology, identification and diagnosis of iron deficiency anaemia, describes sources of dietary iron and factors influencing dietary iron absorption, and discusses patient management. [Abstract copyright: © 2025 RCN Publishing Company Ltd. All rights reserved. Not to be copied, transmitted or recorded in any way, in whole or part, without prior permission of the publishers.