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The self-renewal of mouse embryonic stem cells is regulated by cell-substratum adhesion and cell spreading
No full textMouse embryonic stem cells (mESCs) undergo self-renewal in the presence of the cytokine, leukaemia inhibitory factor (LIF). Following LIF withdrawal, mESCs differentiate, and this is accompanied by an increase in cell–substratum adhesion and cell spreading. The purpose of this study was to investigate the relationship between cell spreading and mESC differentiation. Using E14 and R1 mESC lines, we have restricted cell spreading in the absence of LIF by either culturing mESCs on chemically defined, weakly adhesive biomaterial substrates, or by manipulating the cytoskeleton. We demonstrate that by restricting the degree of spreading by either method, mESCs can be maintained in an undifferentiated and pluripotent state. Under these conditions, self-renewal occurs without the need for LIF and is independent of nuclear translocation of tyrosine-phosphorylated STAT3 or β-catenin, which have previously been implicated in self-renewal. We also demonstrate that the effect of restricted cell spreading on mESC self-renewal is not mediated by increased intercellular adhesion, as evidenced by the observations that inhibition of mESC adhesion using a function blocking anti E-cadherin antibody or siRNA do not promote differentiation. These results show that mESC spreading and differentiation are regulated both by LIF and by cell–substratum adhesion, consistent with the hypothesis that cell spreading is the common intermediate step in the regulation of mESC differentiation by either LIF or cell–substratum adhesion.
The phenomenon of cyberbullying and the criminal justice system
No full textResearch proposal (PhD)--University of South Australia, 2011.
Understanding the factors that influence patient satisfaction with ambulance services
No full textThe quality of ambulance services has an immense impact on patients’ future wellbeing and quality of life. Patient satisfaction is one of the key metrics for evaluating the quality of this service. Yet, the patient satisfaction measurement may be limited in its ability to accurately reflect this service quality, and even reflect factors beyond the patient experiences. The authors analyze 10 years of survey data to reveal a number of factors that systematically bias ambulance satisfaction ratings. Taking into account these biases provides more robust comparison of ambulance performance over time or across different jurisdictions.
O&G Magazine submission on Iodine Deficiency and Pregnancy [Folder Title]
No full textIn this folder it contains documents relating to Basil Hetzel’s article submission on iodine deficiency in pregnancy to The Royal Australian and New Zealand College of Obstetricians & Gynaecologists (O& G) magazine. Documents include: correspondence between O&G magazine and Hetzel before and after his article submission on iodine deficiency in pregnancy; a draft of the article written by Hetzel titled “The Elimination of Brain Damage due to Iodine Deficiency in Pregnancy”; a copy of the O&G magazine vol. 3, no. 3 September 2001 issue where his article is featured.
Version 2 of “Teacher training and re-training for tomorrow”
No full textThis item is a version of a 7 page paper written by Dr Gregor Ramsey and presented to the conference of the technical teachers association, Adelaide in August, 1981. The paper is about the professional development of teachers in the context of recent changes and pressures in the higher education sector.
How do salespeople make decisions? The role of emotions and deliberation on adaptive selling, and the moderating role of intuition
No full textThis research explores how salespeople make decisions and what factors influence these decisions. Research in psychology suggests that, in making decisions, people use both intuition and deliberation, often relying on some degree of both processes. This study examines the impact of emotion, intuition, and deliberation on a salesperson’s adaptability and resulting performance. Intuition is found to play a significant moderating role in the relationships between both deliberation and regulation of emotions on adaptive selling. However, as anticipated, the role of this moderation variable differs for each of these relationships. Findings suggest that intuition provides an important input to deliberative and emotive thought processes, and plays an important role in salesperson adaptiveness. Implications for salesperson mentoring and training programs are explored.
Correspondence from Brian Lee to Dr Gregor Ramsey concerning the second national conference of design education in schools
No full textThis item is 1 page of correspondence from Brian Lee to Dr Gregor Ramsey received on the 13th of August, 1982. The correspondence is an invitation to attend the opening of the second national conference of design education in schools, to be held at the Underdale campus of the South Australian College of advanced education in September of 1982.
U.S. multi-campus systems – Is there a lesson for Australia? A seminar
No full textThis item is a 3 page document consisting of a 2 page notification of a seminar and 1 page attendance registration form intended to be filled out and returned. The seminar is about multi-campus higher education institutions, and speakers at the seminar include Professor John Creswell and Dr Gregor Ramsey.
Basil Hetzel's Foreword in Goitre Monitor: History of Iodine Deficiency Disorders in Tasmania from Pre-British Settlement to the Present Day
No full textThis item is Basil Hetzel’s Forward submission to the “Goitre Monitor: History of Iodine Deficiency Disorders in Tasmania from Pre-British Settlement to the Present Day” titled “History of Iodine Deficiency in Tasmania.”