Jurnal Sain Veteriner
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High Risk Period (HRP) Kasus Penyakit Mulut dan Kuku Pasca Wabah pada Peternakan Sapi Perah di Kabupaten Malang
Get PDFPenyakit Mulut dan Kuku (PMK) merupakan penyakit ternak yang sangat merugikan secara ekonomi. Perhitungan periode berisiko atau high risk period (HRP) akan membantu otoritas veteriner untuk melakukan pelacakan secara efisien potensi wabah sekunder atau penyebaran virus dari kasus indeks. Penelitian ini dilakukan untuk menganalisis perhitungan HRP pada peternakan sapi perah di Kabupaten Malang berdasarkan variabel 1) pengetahuan peternak mengenali gejala klinis PMK, 2) waktu pelaporan peternak, 3) waktu petugas datang menangani pelaporan, dan 4) waktu yang dibutuhkan untuk konfirmasi uji laboratorium. Survei dilakukan pada 126 peternak pada bulan Juli-Agustus 2023. Ukuran sampel dihitung berdasarkan asumsi tingkat kepercayaan 95%, prevalensi dugaan 13%, tingkat kesalahan 6% menggunakan software WinEpiscope 2.0. Data dianalisis secara deskriptif. Peternak sapi perah di Kabupaten Malang memiliki pengetahuan yang baik tentang gejala klinis PMK meski ada beberapa ciri yang luput dan perlu ditingkatkan pemahamannya. Sebanyak 98% peternak segera melakukan pelaporan setelah mengamati adanya gejala klinis PMK pada ternaknya. Kecepatan petugas dalam menanggapi pelaporan sudah baik yakni seketika pada hari pelaporan dilaporkan dan pengujian laboratorium yang segera dilakukan untuk mengonfirmasi penyakit PMK dapat membuat probabilitas HRP 7 hari 91% (SK: 0,87-0,95), HRP 14 hari 7% (SK: 0,04-0,11) dan HRP 21 hari 2% (SK: 0,01-0,05). Pengetahuan mengenai gejala klinis dan kesadaran peternak dalam melakukan pelaporan PMK di Kabupaten Malang sudah baik, namun perlu ditingkatkan. Upaya peningkatan peran peternak dalam deteksi dini dapat dilakukan dengan intervensi berupa penyuluhan dan membangun kedekatan dengan petugas lapangan
Kualitas Spermatozoa Sapi Bali Polled setelah Pemberian Pakan Tersuplementasi Taoge (Phaseolus radiatus L.)
Get PDF The study aimed to increase the sperm quality of polled Bali bulls with the supplementation of bean sprout (Phaseolus radiatus L.) in feeding. This research was used two polled Bali bulls aged 5-6 years old and supplemented with bean sprout 1 kg/bull two times in a week for two months. Semen was collected using artificial vagina method. The evaluation macroscopic of semen was volume while the microscopic evaluation such as motility, viability, abnormality, plasma membrane integrity (PMI), acrosome integrity, and DNA fragmentation. The result of this research showed that semen volume and spermatozoa abnormality of fresh semen after feeding of bean sprouts were not significantly difference (P>0.05) compared to before feeding, but motility and viability were significantly higher (P0.05) while viability and PMI were significant higher (P<0.01), abnormality and DNA Fragmentation were significant lower (P<0.01) after feeding compared to before. The research revealed that the supplementation of bean sprouts could increase the motility and viability but had no effect on volume and abnormality of fresh semen. Meanwhile in frozen semen, it increased viability and PMI and decreased abnormality and DNA fragmentation but had no effect on motility and acrosome integrity
Pengaruh Penggunaan Grid terhadap Kualitas Hasil Rontgen Anjing dan Kucing Pasien RSH Prof. Soeparwi FKH UGM
Get PDFDokter hewan praktisi sering menjumpai permasalahan dalam penegakan diagnosis, salah satunya adalah pemanfaatan rontgen atau hasil X-ray yang belum optimal. Kualitas radiografi itu sendiri meliputi densitas, kontras, ketajaman dan distorsi, sedangkan salah satu penyebab yang dapat menurunkan kualitas radiografi adalah radiasi hambur. Penelitian ini bertujuan untuk meningkatkan kualitas radiograf dengan mengurangi radiasi hambur sinar-x menggunakan grid. Penelitian ini menggunakan 10 ekor anjing dan 10 ekor kucing dengan berat badan bervariasi dan fokus rontgen yang berbeda. Setiap hewan dilakukan pengambilan sampel xray sebanyak 2 kali, yaitu foto rontgen menggunakan grid dan tanpa grid pada posisi lateral dan anterior-posterior. Hasil rontgen dibandingkan antara foto rontgen dengan grid dan tanpa grid. Dari penelitian ini diperoleh hasil bahwa pengujian foto rontgen menggunakan grid mampu mengurangi radiasi hambur sehingga lebih mengoptimalkan radiograf dan membantu dokter hewan dalam penegakan diagnosa
Pengaruh Pemberian Feed Additive Terhadap Jumlah Erirosit, Kadar Hemoglobin dan Nilai Hematokrit Pedet Jantan di KSPTP Fakultas Peternakan Universitas Padjadjaran
Get PDFPenelitian ini bertujuan untuk mengetahui pengaruh pemberian feed additive terhadap jumlah eritrosit, kadar hemoglobin dan nilai hematokrit pedet peranakan Friesian Holstein (PFH). Penelitian ini menggunakan pedet PFH jantan sebanyak 16 ekor dibagi menjadi 2 kelompok berdasarkan umur yaitu 4 minggu (n=8 ekor) dan 12 minggu (n=8 ekor). Penelitian ini menggunakan rancangan acak kelompok. Masing – masing kelompok umur diberi 4 perlakuan berbagai dosis pemberian feed additive, sehingga terdapat 8 kelompok perlakuan. Setiap kelompok perlakuan terdiri dari 2 ekor pedet. Dosis perlakuan pemberian feed additive adalah sebagai berikut: 0 ml (kontrol atau P0); 0,5 ml (P1); 1 ml (P2); dan 1,5 ml (P3). Pengambilan sampel darah dilakukan sebanyak tiga kali (sebelum, 30 hari dan 60 hari) selama dua bulan pemeriharaan. Data dianalisis statistik menggunakan sidik ragam dan dilanjutkan dengan uji berganda Duncan. Hasil penelitian menunjukkan bahwa pemberian berbagai dosis feed additive tidak berbeda nyata terhadap jumlah eritrosit, kadar hemoglobin dan nilai hematokrit (P> 0,05) pada pedet. Namun, kelompok umur berpengaruh nyata terhadap rataan jumlah eritrosit dan kadar hemoglobin (P<0,05). Pemberian 1,5 ml feed addirive mampu meningkatkan profil hematologi dalam kisaran normal
Melacak Gen Faktor Virulensi Escherichia coli yang Tahan terhadap Siprofloksasin asal Usap Kloaka Ayam Petelur
Get PDFAvian Pathogenic Escherichia coli (APEC) is a cause of extraintestinal infections, particularly respiratory infections, pericarditis, and septicemia in poultry (colibacillosis). Research aimed at uncovering the virulence factors responsible for colibacillosis in poultry, especially laying hens in Indonesia, is still quite rare. The purpose of this study is to identify the virulence factors responsible for colibacillosis in E. coli strains resistant to ciprofloxacin. The virulence factors targeted in this research are the genes iss, iutA, iroN, ompT, and hlyF. The study utilized 327 E. coli isolates from the archives of the National Veterinary Drug Assay Laboratory, which were collected in 2021 in seven provinces.All isolates were tested for pathogenicity using Congo Red. Subsequently, E. coli strains determined to be pathogenic were tested for their sensitivity to ciprofloxacin using the agar dilution method. Isolates that were both resistant and pathogenic were then examined for the presence of the virulence genes using polymerase chain reaction (PCR). Based on the results of the Congo Red test, it was found that 59 isolates (18%) were pathogenic E. coli. Among the 54 pathogenic E. coli isolates subjected to sensitivity testing, 30 isolates were found to be resistant to ciprofloxacin. Subsequently, PCR analysis of these 30 ciprofloxacin-resistant pathogenic E. coli isolates revealed the presence of the iss gene in 29 isolates (96%), iutA in 23 isolates (76.6%), ompT in 19 isolates (63.3%), hlyF in 14 isolates (46.6%), and iroN in 11 isolates (36.6%). Eight isolates showed the presence of all five virulence genes, while the rest had one to three virulence genes. This study demonstrates that virulence genes are commonly found in ciprofloxacin-resistant pathogenic E. coli. This suggests a potential threat to the effectiveness of ciprofloxacin in managing colibacillosis in laying hens.
Performa Estrus dan Hormon Estrogen Sapi Friesian Holstein Postpartus yang Diberi Urea Multinutrient Molasses Block (UMMB) dengan Perekat Tepung Tapioka
Get PDFThe reproductive ability of Holstein Friesian (FH) cows greatly affects milk yield because, however, in the lactation period, dairy cows anestrus postpartus, which is the condition where does not appear in estrus postpartus. Anestrus postpartus to uterine involution as a consequence of nutritional deficiencies. Recovery from can be this condition can be conducted by additional feeding. One of the supplementary feeds is urea multinutrient molasses block (UMMB). UMMB consists of fillers and adhesives and cement is the most used in UMMB production as an adhesive. UMMB is an additional feed using cement dairy cows health problems. Therefore, the objective of this research was to determine the effect of cement substitution with tapioca meal as an adhesive on postpartus estrus of FH cows. A total of 9 lactating FH dairy cows were divided into 3 dietary groups. Each treatment had 3 cows as replicates. The dietary treatments consisted of UMMB with 100% cement adhesive (as D0); 50% cement and 50% tapioca (D1); and 0% cement and 100% tapioca meal (D2). The parameters were postpartus estrus, estrus speed, estrus duration, estrus intensity, and estrogen hormone levels. The results revealed that the treatment had a significant effect (P0.05) neither on postpartus estrus, estrus speed, estrus intensity, or estrogen hormone levels. Therefore, it can be concluded that tapioca meals can be used as a substitute for cement as much as 50% provides as an adhesive in UMMB production for better estrus performance
Komparasi Metode coa Polymorphism dan coa Typing pada Bakteri Staphylococcus aureus yang Diisolasi dari Sumber Berbeda
Get PDFStaphylococcus aureus (S. aureus) merupakan golongan bakteri koagulase positif yang menyebabkan berbagai macam penyakit klinis pada manusia dan hewan. Determinasi pola gen coa merupakan salah satu metode yang sering digunakan dalam lingkup studi epidemiologi S. aureus. Penelitian ini dilakukan untuk membandingkan metode coa polymorphism dan coa typing pada isolat S. aureus yang diisolasi dari sumber berbeda. Tujuh belas koleksi isolat S. aureus asal susu pasteurisasi, susu mastitis kambing peranakan etawa, dan daging ayam segar ditumbuhkan dari stok gliserol -80oC, kemudian dilakukan ekstraksi DNA dan amplifikasi gen penyandi koagulase (coa) dengan teknik PCR menggunakan primer untuk coa polymorphism dan coa typing. Diketahui, level diskriminasi coa typing lebih variatif dibandingkan dengan coa polymorphism pada 17 isolat yang diuji. Metode coa typing menunjukkan 7 tipe berbeda, sedangkan coa polymorphism hanya 3. Empat dari 17 isolat tidak dapat ditentukan coa typing, menunjukkan potensi pengembangan tipe baru coa typing untuk isolat-isolat asal Indonesia, untuk kepentingan studi epidemiologi
Development of In-House ELISA using recombinant LipL32 for Detection of Human Leptospirosis in Indonesia
Get PDFEarly laboratory confirmation is important for the accurate diagnosis and treatment of patient infected by leptospirosis. However, Microscopic agglutination test (MAT) as the gold standard for detection of human leptospirosis has many limitation and only available in reference laboratories. Therefore, many studies suggested LipL32 protein as a good candidate for development of leptospirosis detection kit because it is highly conserved and produced only in pathogenic Leptospira species. In this study, we aim to investigate the performance of our in-house ELISA using recombinant LipL32 to detect leptospirosis in Indonesia. Fourteen human sera were used in this study and the infection status were determine using MAT. The result showed that nine of eleven MAT positive sera were successfully recognized by LipL32 ELISA. The antibody binding to LipL32 was also confirm by immunoblot. There was one of three MAT negative sera has high OD above 0.5 in ELISA, but it showed negative reaction in immunoblot result. Overall, this study demonstrated that recombinant LipL32 protein can recognized antibody from human leptospirosis and can be used as a universal antigen to detect infection by any serovars of pathogenic leptospira
Aktivitas Madu sebagai Antibakteri Staphylococcus aureusdan Escherichia ColiO157:H7
Get PDFHoney is a natural substance from flower nectar and produced by honey bees. The honey has been used as healing. The study was conducted to determine the antibacterial activity of Blora and Kaliandra honey against pathogenic bacteria such as Staphylococcus aureus (S. aureus) and Escherichia coli O157:H7 (E. coli O157:H7). As a comparison, was used commercial honey. The results showed Blora and Kaliandra honey had antibacterial activity, however the commercial honey has not antibacterial activity. Kaliandra honey has antibacterial activity against S. aureus and E. coli O157:H7 better than Blora and commercial honey. Concentration 25% of Kaliandra honey was able to inhibit S. aureus, while blora honey showed antibacterial activity at 50% and commercial honey did not show antibacterial activity until concentration 50%. Inhibition zone of S. aureus produced by Kaliandra honey with concentration 25 and 50% were 8.0 ± 0.1 mm and 9.0 ± 0.2 mm respectively and concentration 50% Blora honey was 7.3 ± 0.1 mm. Inhibition zone of E. coli O157:H7 produced by concentration 50% Kaliandra honey was 8.0 ± 0.3 mm and Blora honey was 7.7 ± 0.1 mm. The study showed that Blora and Kaliandra honey have antibacterial activity and can be used as alternative substitution in antibiotic therapy
Identification of Bovine Rotavirus Group A in Bogor, West Java
Get PDFAbstrak Diare merupakan penyakit yang menyebabkan angka kesakitan yang tinggi pada pedet dan kematian neonatal. Penyakit ini dapat disebabkan oleh beberapa agen penyakit yang berbeda. Rotavirus Grup A (RVA) atau Bovine Rotavirus merupakan salah satu agen infeksi penyebab diare pada pedet. Selanjutnya, diare neonatal pada pedet dapat berdampak pada kerugian ekonomi bagi ternak sapi perah dan sapi potong di seluruh dunia karena menyebabkan gangguan pertumbuhan, meningkatnya biaya perawatan, dan/atau kematian pada hewan sakit. Prevalensi Bovine Rotavirus dapat berbeda antar negara di seluruh dunia. Sirkulasi Bovine Rotavirus pada sapi telah dilaporkan di beberapa negara tetapi sirkulasi Bovine Rotavirus pada sapi di Indonesia belum diketahui. Untuk mengetahui prevalensi Rotavirus grup A atau Bovine Rotavirus (BRV), 100 sampel feses dikoleksi dari pedet dengan gejala klinis diare atau tidak diare di Kabupaten Bogor, Jawa Barat pada tahun 2021. Sampel dianalisis terhadap urutan yang mengkode protein kapsid bagian dalam VP6 (subkelompok) menggunakan Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Lima dari 100 sampel feses sapi (5%) terdeteksi positif BRV. Pada penelitian ini menunjukkan bahwa kelompok Rotavirus atau Bovine Rotavirus (BRV) telah bersirkulasi di antara ternak sapi di Indonesia, khususnya Kabupaten Bogor. Sampel positif Rotavirus grup A atau Bovine Rotavirus (BRV) dapat diidentifikasi dengan metode diagnosis dini (RT-PCR). Kata kunci : Bovine Rotavirus; RT PCR; Bogor Abstract Diarrhea is the most disease that cause high morbidity in calves and neonatal mortality. This disease can be caused by several different infectious agents. Group A rotaviruses (RVA) or Bovine Rotavirus are one of the infectious agents causing diarrhea in calves. Then, Neonatal calf diarrhea can impact to economic losses to dairy and beef cattle herds worldwide, in consequence of growth disorders, value of treatment, and/or death of sick animals. The prevalence of Bovine Rotavirus can become different in the worldwide. The circulation of these bovine rotavirus in calves from the some region has already been demonstrated but the circulation of bovine rotavirus in Indonesia is not known. To investigate the prevalence of A group Rotaviruses or Bovine Rotavirus (BRV), 100 fecal samples were collected from calves with diarrhea or no diarrhea in Bogor district, West Java at 2021. The samples were analyzed for sequences encoding the inner capsid protein VP6 (subgroup) using RT-PCR. Five of 100 specimens of bovine fecal (5%) were detected positive as BRV positive. In this study, A group Rotaviruses or Bovine Rotavirus (BRV) have been circulated among cattle herds in Indonesia, particularly Bogor District. The positive samples of A group Rotaviruses or Bovine Rotavirus (BRV) can be identified using the early diagnosis method (RT-PCR). Keywords : Bovine Rotavirus; RT PCR; Bogor Distric