Jurnal Sain Veteriner
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Prolapsus Rektum Pada Domba Dorper (Case Report: Rectal Prolapses in Dorper Sheep)
No full textProlaps rektum merupakan tonjolan jaringan rektal di luar anus. Faktor penyebab prolaps rektum pada hewan muda dan tua meliputi konstipasi, endoparasit, diare, faktor keturunan, kehilangan daya spinchter ani dan pelonggaran selaput lendir rektum. Hewan kasus adalah tiga ekor domba Dorper berasal dari Wonosari, Gunungkidul dengan keluhan prolaps rektum selama 3-4 hari, nafsu makan menurun dan domba masih aktif. Domba pertama betina 50 kg berusia 2.5 tahun, domba kedua betina 30 kg berusia 5 bulan, dan domba ketiga jantan 35 kg berusia 5 bulan. Pemeriksaan fisik menunjukkan gejala klinis primer prolapsus rektum berupa massa silindris memanjang yang menonjol melalui lubang anus, bagian proksimal rectum berwarna merah muda sedangkan bagian distal berwarna merah, serta terdapat peradangan pada mukosa rektum. Tindakan operasi dilakukan untuk reposisi rektum. Premedikasi menggunakan Acepromazine, dilanjutkan injeksi anestesi epidural menggunakan Lidocaine. Anestesi lokal Lidocaine line block diinjeksikan di sekitar anus. Rektum dimasukkan perlahan secara manual, kemudian dilakukan penjahitan di sekeliling anus dengan pola purse string agar rektum tidak kembali prolaps. Terapi post operatif diberikan injeksi antibiotik broad spectrum Amoxicillin dengan sediaan 150 mg/ml (1 ml/10kg BB) dan injeksi antiinflamasi (NSAIDs) Flunixin Meglumine 50 mg/ml (0,02-0,04 ml/kg BB; q12h). Domba Dorper jantan dipotong karena mengalami prolaps rektum berulang dan lemah.
Isolation of Aspergillus from edible-nest swiftlet feces in bird houses of Central Java
No full textFive edible-nest swiftlet houses in Central Java were sampled for fecal specimens. The samples were obtained using a sterile container to maintain aseptic conditions. Subsequently, the samples were mixed thoroughly in a tube containing sterile saline and allowed to incubate for a duration of 30 minutes. One milliliter of supernatant was placed onto Potato Dextrose Agar (PDA) with chloramphenicol (50 mg/L) and incubated at a temperature of 25 ºC for a duration of one week. The results showed that all samples had a high percentage of Aspergillus niger (100%), Aspergillus flavus (100%), and Aspergillus fumigatus (100%) isolates. The findings suggest that edible-nest swiftlet houses in Central Java can serve as a potential reservoir and conduit for fungal infection and transmission
Pengaruh Pemberian Ekstrak Etanol Kulit Buah Jeruk Baby (Citrus sinensis L. Osbeck) Selama 28 hari terhadap Kadar SGOT dan SGPT
Full text linkOne of the plants used as natural medicine is baby orange (Citrus sinensis L. Osbeck). Baby oranges contain hesperidin, hesperetin, narirutin and nobiletin compounds. The purpose of this study was to determine the effect of giving ethanol extract of baby orange peel for 28 days on liver function as seen from the increase in SGOT and SGPT levels. This research is a pure experimental study with a completely randomized design (CRD) with a unidirectional pattern, namely the method used to observe the relationship of independent variables (including: normal control, negative control, ethanol extract of baby orange peel (EEKBJB) doses of 50, 100, 200 and 400 mg/kg BW) with the dependent variable (SGOT and SGPT levels). The test animals used in this study were 30 male Wistar rats which were divided into 6 treatment groups which were treated for 28 consecutive days. On day 29, blood samples were taken from the orbital sinus of the eye and the levels of SGOT and SGPT were measured. There was a significant difference in the levels of SGOT and SGPT as indicated by the results of the One Way ANOVA test (p<0.05). The results of the Post Hoc-Tukey test for SGOT levels showed a significant difference between the control group and the 50 mg/kg BW dose group, while the 100, 200 and 400 mg/kg BW doses did not show a significant difference. The results of the Post Hoc-Tukey, s-HSD test for SGPT levels did not show a significant difference between the control group and the 50, 100, 200 and 400 mg/kg BW dose groups. Significant differences were shown by doses of 50 and 400 mg/kg BW. Giving EEKBJB for 28 days did not cause toxic effects on liver function, seen from the absence of an increase in SGOT and SGPT levels
Kristaluria disertai Azotemia dan Uremia pada Kucing
Full text linkABSTRAKKristaluria merupakan salah satu gangguan yang sering ditemukan pada kucing, yang apabila tidak segera ditangani dapat menyebabkan kematian. Laporan kasus ini menyampaikan diagnosis dan terapi kucing penderita kristaluria disertai Azotemia dan Uremia. Laporan kasus ini menggunakan kucing mixdom, jantan, berumur dua tahun, bobot badan 6 kg, yang diperiksakan karena kucing tidak dapat urinasi selama 5 hari disertai nafsu makan dan minum menurun. Kucing diperiksa secara fisik dan laboratorik, serta diterapi berdasar hasil diagnosis. Hasil pemeriksaan fisik menunjukkan ekspresi muka waspada, kondisi tubuh sangat gemuk dengan body condition score (BCS) 5/5. Frekuensi nafas 80x/menit; frekuensi pulsus 116x/menit; dan suhu tubuh 38,2°C. Palpasi vesica urinaria (VU) kucing teraba mengalami distensi dan terdapat respon nyeri. Saat dilakukan palpasi VU, urin dapat keluar dan tampak berwarna kemerahan (hematuria). Pemeriksaan urin di bawah mikroskop terlihat adanya kristal magnesium amonium fosfat (struvite). Urinalisis menunjukkan kucing mengalami leukosituria, proteinuria, glukosuria, dan hematuria. Pemeriksaan hematologi dan kimia darah menunjukkan kucing mengalami anemia normositik-normokromik, leukositosis dengan neutrofilia dan limfopenia, azotemia, dan uremia. Kucing didiagnosis mengalami kristaluria disertai azotemia dan uremia dengan prognosis dubius-infausta. Terapi yang diberikan adalah pemijatan VU sampai urin keluar dan VU teraba kosong. Pasien diberikan terapi Amoxicillin 10% dengan dosis 10 mg/kg bb, IM, 1 x pemberian, diphenhydramine HCl dengan dosis 1 mg/kg bb, IM 1x pemberian. Selanjutnya diberikan amoxicillin secara oral dengan dosis 12,5 mg/kg bb,diberikan 2x sehari. Obat oral Shi Lin Tong 2x sehari sebanyak 2 tablet. Kondisi kucing menurun di hari kedua pengobatan dan berujung kematian. Perubahan makroskopis organ yang teramati yaitu pulmo mengalami edema dan hemoragi, vesica urinaria mengalami distensi dan hemoragi, ginjal mengalami hemoragi pada corticomedullary junction. Hasil pemeriksaan histopatologis menunjukkan adanya perubahan/gangguan pada vesika urinaria berupa nekrosis sebagian epithel mukosa, hemoragi subepithelial, oedema submukosa, infiltasi netrofil dan limfosit di tunika mukosa sampai tunika muskularis secara diffuse Pada ginjal terlihat vakuola berbatas jelas di sitoplasama epithel tubuli derajat berat, masa homogen eosinofilik dalam jumlah sedang di lumen tubuli. Sebagian kecil epithel tubuli mengalami nekrosis. Kongesti kapiler intraglomerular. Ginjal juga terlihat dilatasi lumen tubuli dengan epithel yang memipih. Urethra terlihat hemoragi (ekstravasasi eritrosit) di subepithelial tunika mukosa disertai infiltrasi limfosit dan neutrophil di tunika mukosa sampai submucosa Gambaran nekropsi pada organ vesica urinaria, ginjal dan urethra mendukung diagnosis berdasar pemeriksaan antemortem. Kata-kata kunci: kristaluria, azotemia, uremia, struvit
Optimization of Sybr Green Quantitative Real Time Polymerase Chain Reaction (qPCR) using Excreted-Secreted Antigens (ESAs) Genetik Marker for Detection Toxoplasma gondii
Full text linkAbstractToxoplasma gondii is an obligate intracellular parasite, causing toxoplasmosis in almost all warm-blooded animals and humans worldwide. Toxoplasmosis is a zoonotic disease of serious public health concern. Host cell invasion by T. gondii tachyzoites has process involving the sequential secretion of Excreted-Secreted Antigens (ESAs). T. gondi ESAs could be a valuable candidate for the diagnosis of toxoplasmosis. Techniques to more accurately detection of T. gondii recently developed biotechnological methods that are currently being used, conventional and real time Polymerase Chain Reaction (RT-PCR). RT-PCR is more widely used because it is more sensitive and specific. The aims of this study were to optimize the Sybr Green RT-PCR in different region gene based on Excreted-Secreted Antigens (ESAs), tachyzoite surface antigen and bradhyzoite antige, then adapt the conventional PCR program to real-time PCR for detection Toxoplasma gondii. Optimization is necessary to get optimal condition of PCR to get the best results. T. gondii RH strains derived from liquid nitrogen and DNA extracted by DNAzol. The genetic marker used GRA1#1, GRA1#2, GRA7#1, GRA7#2, ROP1, MIC3, SAG1 and BAG1. The results of the optimization of multiple primer genes can adapt and be used optimal in RT-PCR by using the same cycle program simultaneously in one run. Overall, RT-PCR for the detection of T. gondii DNA demonstrated excellent agreement with conventional PCR. RT-PCR with melting curve analysis is rapid and simple that facilitates high throughput analysis to detect T. gondii. The optimal conditions obtained from the optimization results can facilitate further research to detect T. gondii.Keywords: Biotechnology molecular, Detection, excretory-secretory antigen, toxoplasmosi
Histological Structure of Spleen White Rat (Rattus norvegicus L.) After Induced of Nanochitosan Preparation of Neem Leaf (Azadirachta indica A. Juss) Ethanol Extract
No full textAgents of immunomodulation are compounds that have the ability to balance the immune system throughout the body. The neem plant is one of the herbal plants that is often used as traditional medicine. Neem leaves (Azadirachta indica A. Juss) contain antioxidants that have the potential to be antiinflammatory, antiviral, and as an immunomodulatory agent. The constraint in giving medicine orally is the low bioavailability of drugs and the distribution of active compounds of herbal plants. This problem can be solved by packaging herbal plant extracts in nanochitosan. The purpose of this study was to analyze the effect of induced of a nanochitosan ethanol extract of neem leaves on the histological structure of the spleen tissue of female Wistar white rats. This study used a randomized design (CRD) which was divided into 3 treatment groups with 4 repetitions. Treatments include control (P0), an ethanol extract of neem leaves 2 ml/head/day (P1), and nanochitosan preparation of neem leaves 2 ml/head/day (P2). The parameters measured were the diameter of white pulp, the diameter of germinal centre, the area of white pulp, and the area of germinal centre in the spleen. Data on the diameter and the area of the white pulp and germinal centre were analyzed statistically parametrically with ANOVA with a 95% level of confidence, if the data were not normally distributed, the Kruskall Wallis nonparametric test was carried out. Data that were significantly different were carried out by Duncan’s further test. The results showed that the treatment of nanochitosan preparations of neem leaf ethanol extract had a significant difference in the diameter of the white pulp and germinal centre as well as the area of the white pulp and germinal centre (P<0,05). The conclusion of this study was that the nanochitosan preparation ethanol extract of neem leaves at a dose of 14 mg/kg BB can increase the diameter and the area of the white pulp and germinal centre
Aktivitas Sitotoksik Ekstrak Keong Laut Matah Merah (Cerithidea obtusa) terhadap Sel Kanker Kolon WiDr
Full text linkBased on data released by Globocan in 2020, the incidence of colorectal cancer is the fourth highest in Indonesia (8.6%) and the third in the world (10%). This disease is hard to treat because the available therapy is less effective. Therefore, it is necessary to develop effective alternative therapies, especially those originating from Indonesia's natural resources, easy to obtain and reproduce. This study aims to determine the potential of red eye snail extract as an anticancer through cytotoxicity tests with the MTT Assay method on colon cancer cells WiDr and DNA fragmentation tests with Hoescth staining. In this study, we used various concentrations of the red-eye snail extract to test with 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) and doxorubicin reagents as positive controls. Absorbance values were read using a microplate reader at a wavelength of 595 nm. The cell absorbance data was converted into cell viability and probit analyzed to obtain the IC50 value. The results showed that the higher the concentration of the extract caused a decrease in the number of WiDr cells and an increase in damage to the structure of WiDr cells. Based on the results of probit analysis, it was found that the IC50 value of the extract was 36.28 µg/mL or classified as moderate cytotoxicity. The DNA fragmentation test showed that at concentrations of 125 ppm and 62.5 ppm, it was able to provide an effect similar to doxorubicin, namely triggering apoptosis in WiDr colon cancer cells
Analysis of Storage Time for Merawang Chicken Eggs (Gallus Gallus) against Hatchability: A Systematic Review
Full text linkBackground: Merawang chicken has the potential to be developed, both as local laying hens and local broilers. Hatchability and quality of hatching eggs are influenced by storage method, storage time, storage area, ambient temperature, incubator temperature, reversal during hatching. Storage that is too long causes the quality and hatchability to decrease so eggs should be stored no more than 7 days. Purpose: The purpose of this study was to analyze the length of storage of Merawang chicken eggs (Gallus gallus) on hatchability. Research methods: A literature search was carried out systematically through the PubMed, NCBI, Google Scholar databases using keywords, namely, "Storage Period, Chicken Eggs, Merawang (Gallus Gallus), Hatchability". Based on these keywords, the articles obtained were first selected by setting several inclusion criteria. Research results: Based on the search results in the PubMed, NCBI, Google Scholar databases using predetermined keywords, 15400 articles were obtained for the keywords “Storage Time of Merawang Chicken Eggs (Gallus Gallus), Hatching Power”. All articles were reselected based on inclusion criteria and exclusion and obtained as many as 23 articles that met the inclusion criteria. Conclusion: From the results it can be concluded that the storage time is 9 days (P5) showed hatchability, high fertility and lowest embryo mortality. Need research on the other animal for future research
Studi Perilaku Harian Rusa Timor (Cervus timorensis) di Lembaga Konservasi PT Taman Satwa Semarang
Full text linkAdaptation of timor deer at the Conservation Institution of PT Taman Satwa Semarang is formed through a learning and memory process. The presence of visitors stimulates the deer to walk towards the side of the pen even though it is resting. Drop in feeding by keepers in the morning, afternoon, and evening causes the deer to become more active during the day. This study aims to analyze frequency and duration of the daily behavior of timor deer on weekdays with few visitors and on weekends with lots of visitors. Behaviors observed include moving, feeding, resting, grooming, aggressive, eliminative, and reproductive behavior. This research was conducted for 30 days. The object used in this study was a population of 18 adult timor deer. The method used is scan sampling, data recording of daily behavior which includes duration and frequency are carried out periodically at 10-minute intervals. The collected data includes daily behavioral, type of feed, as well as temperature and humidity. Data analysis was performed using t-test at a significance level of 5%. The results showed that visitor activity between weekdays and weekends had a significant effect (p˂0.05) on moving, feeding, resting, grooming, aggressive, and reproductive behavior. The conclusion of this study was the most timor deer perform resting and reproductive behaviors on weekdays, while moving, eating, and social behavior are mostly carried out on weekend
Kejadian Rabbit Haemorrhagic Disease (RHD) di Peternakan Kelinci Lokal, Kabupaten Kulon Progo, Provinsi Daerah Istimewa Yogyakarta
Full text linkRabbit Haemorrhagic Disease (RHD) adalah penyakit hepatitis yang fatal dan sangat menular pada kelinci domestik ataupun kelinci liar. Penyakit ini disebabkan oleh Calicivirus, genus Lagovirus, dan famili Caliciviridae. Keberadaan RHD ini telah dilaporkan sebelumnya di negara tetangga seperti Singapura pada tahun 2020. Kasus seropositif RHD pertama kali dilaporkan di Indonesia tahun 2022 pada peternakan kelinci di daerah Lembang, Bandung, Jawa Barat. Provinsi Daerah Istimewa Yogyakarta (DIY) merupakan salah satu sentra ternak kelinci terbesar ke empat di Pulau Jawa selain Jawa Tengah, Jawa Timur, dan Jawa Barat. Penelitian ini bertujuan mengetahui keberadaan RHD melalui deteksi serologi di sentra peternakan kelinci lokal di Kabupaten Kulon Progo. Sejumlah 59 ekor kelinci dari peternakan kelinci lokal di 6 kecamatan di Kabupaten Kulon Progo diperiksa keberadaan antibodi anti RHD nya. Keenam kecamatan tersebut merupakan sentra peternakan kelinci di Kabupaten Kulon Progo yaitu Kecamatan Pengasih, Nanggulan, Kalibawang, Sentolo, Panjatan, dan Girimulyo. Serum diuji menggunakan metode Indirect Enzyme-Linked Immunosorbent Assay (ELISA) untuk mengetahui titer antibodi terhadap RHD. Berdasarkan hasil pengujian ELISA diperoleh hasil 5 dari 59 sampel serum kelinci menunjukkan hasil seropositif terhadap RHD. Adanya temuan hasil seropositif RHD tersebut menjadi bukti bahwa terdapat infeksi RHD pada kelinci di peternakan kelinci lokal, Kabupaten Kulon Progo yang diindikasikan dari adanya antibodi terhadap RHD