Jurnal Sain Veteriner
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Blood Profile of Wistar Rats due to Subcronic Condition Caused by Sodium Nitrite
Get PDFAbstract Nowadays, chemicals used as food additives are increasing. One of the chemicals commonly used is sodium nitrite (NaNO2 ). Sodium nitrite is found in sausage and another meat product. Sodium nitrite is used as food preservative agent. But, the presence of NaNO2 can influence blood cells ability to carry oxygen. It causes anemia and forms nitrosamines, carcinogenic agents. This research is purposed to know blood profiles of Wistar rats on subchronic condition caused by NaNO2 . The research was done for 3 months as subchronic time. Twenty seven Wistar rats were divided into 3 groups, control group (K), first group (P1) treated by NaNO2 with dose of 11,25 mg/kg bodyweight/day, and second group (P2) treated by NaNO2 with dose of 22,50 mg/kg bodyweight/day. Blood samples were collected from orbital sinus and tested once a week for hematological parameters, including white blood cells (WBC), red blood cells (RBC), hemoglobin (Hb), hematocrit (HCT), lymphocyte level, and neutrophil level. The data were analyzed with ANOVA using SPSS 16 program for Windows and the blood profiles alteration were seen. The results from this study showed there were no significant differences among the blood profiles of Wistar rats treated by NaNO2 with dose of 11,25 mg/kg 2 bodyweight/day and dose of 22,50 mg/kg bodyweight/day compared to that of the control group, except WBC values at subchronic condition at dose of 22,50 mg/kg bodyweight/day which decreased 23,46%. The NaNO 2 could not influence the blood profiles of rats treated by NaNO2 subchronically.
Morphological Characterization and genetic Diversity of Macrobrachium rosenbergii (De Man, 1879) Crossbreeding Result from Samas, Bone, and Sintetis Populations
Get PDFAbstract In Indonesia, research for the prime seed of Macrobrachium rosenbergii crossbreeding is rarely done. The aims of this study are to study F1's morphological character and genetic diversity from the result of crossbreeding between M.rosenbergii Samas, Bone, and Sintetis populations. In this study, genetic characterization was known by using PCR RAPD method utilizes three primers and morphological characterization. Data were analysed with UPGMA algorithm and Simple Matching coefficient that were presented in dendrogram. F1's coefficient heterosis was counted based on ratio from the average of cephalothorax and abdoment lenght and also ratio from the average of standard length of charapax and the average of abdomen length. The results showed that there was a very high genetic diversity in F1 population. The specific locus was found in individuals from Sintetis and Samsam populations.100% Polimorfism was found from F1's DNA amplification result, meanwhile monomorphism 50% was found from parent's DNA amplification result on OPA 20. The highest F1 heterosis was found in Samas and Sintetis genotipe
Ovulatory Follicular Dynamics After Estrus Synchronization using Prostaglandin F2a in Dairy Cows
Get PDFThe study aimed to follow development of ovulatory follicular dynamics as well as plasma progesterone profile after estrus synchronization using PGF2 and GnRH. A total of 15 non-pregnant dairy cows, 4-5 years of age, healthy and reproductively sound were used in the present study. Treatment 1, given intramuscular injection of PGF2 25 mg (PGF2), treatment 2 PGF2 25 mg and GnRH 250 g 2 days later (PGF2-GnRH), and treatment 3 with GnRH 250 g (7 days prior to injection of PGF2), PGF2 25 mg and GnRH 250 g (2 days after injection of PGF2) (GnRH-PGF2a-GnRH) (the Ovsynch method). Transrectal ultrasonographic examination using real time, B-mode, with 7.5 MHz tranducer was performed everyday for 12 days to follow ovulatory follicular and luteal dynamics. Blood plasma was taken every day for progesterone determination using EIA technique. Data of follicular, luteal development and progesterone levels were tested using analysis of variance and correlation analysis. The animals showed estrus within 70.70 + 01.90 hours following PGF2 injection. Prostaglandin F2 induced corpus luteum regression, decreased in progesterone plasma levels, followed by ovulatory follicular development and eventually underwent ovulation. Administration of first GnRH increased corpus luteum size, enhanced its regression and decreased plasma progesterone levels, while the second administration induce better ovulatory follicular development. Rate of the corpus luteum regression, progesterone decrease and ovulatory follicular development following PGF2 injection for respective treatments 1, 2 and 3 were 2.53 + 0.24a, 2.73 + 0.36a and 3.53 + 0.28b mm/day; 1.39 + 0.14a, 1.35 + 0.18a dan 1.57 + 0.12b ng/ml/day; and 1.33 + 0.15a, 1.63 + 0.19b and 1.67 + 0.23b mm/day, respectively (P < 0.05). It can be concluded that PGF2 induced corpus luteum regression, decreased in progesterone plasma levels and ovulatory follicular development. Addition of GnRH increased corpus luteum size and plasma progesterone levels, after PGF2 injection corpus luteum regression and progesterone decrease became more prominent, while ovulatory folliculkar development occurred much better. .
Review of The Artificial Insemination Result Based on Anestrus Post Insemination in Bali Cattle Herds at The Regency of Sikka, East Nusa Tenggara
Get PDFAnestrus is one of indirect clinical signs of cattle pregnancy. Based on the present of sufficient progesterone on the blood, the cattle with pregnancy condition will not show the signs of estrus. The success of artificial insemination (AI) is the occurrence of fertilization followed by pregnancy and parturition. This involves a complex relationship between semen quality and oocytes, estrus detection, AI punctuality, inseminator and farmers ability. Artificial insemination should be performed at the relative optimum time to ovulation. Estrus detection is an important factor to determine the time of AI. Estrus Detection of Bali cattles of the farmer is often difficult observed or not observed, especially in semi-intensive system maintenance. From this review it can be seen that there is the different proportion of inseminators on the incidence of estrus after AI. However, determination of the role of inseminator on the success of AI and then the other factors that affect the success of the AI should be under ideal conditions. Straw from the different bulls breed gave the same of AI result. The estimate of pregnancy rate of 80 cows after AI is 55 %. Estrus detection on 21st days after AI can be used as a detection method for early diagnosis of pregnancy, especially for the farmers who did not have any ability or authority to perform medical acts of reproduction which is only owned by a veterinarian. Observation, recording and evaluation of the implementation of the AI should be made until the end of pregnancy followed by the process of parturition
Hypertropic Osteopathy (Marie Diseases) in The German Shepherd Dog
No full textAbstract A Male German Shepherd dog, five years old, twenty six kg of body weight was submitted to Professor Soeparwi Animal Hospital, Faculty of Veterinary Medicine, Gadjah Mada University by Mr. Siswo Han, the owner who lives in Cangkringan, Yogyakarta on May 13, 2013. The dog was suffered from the pain and lameness on the front-left extremity for about 2 weeks. The dog having registered number of 8738/V/A/RSH/13 did not show any specific clinical signs, except the lameness. Skin and hairs, mucous membranes, lymphoid glands, and respiratory, digestive, circulatory and uropoetica organs as well were within the normal limits. The result of standard blood test for both red blood cell and white blood cell were still within normal values. Evaluation of fecal sample of the dog was also normal meaning that no parasites were found in the fecal sample. Radiographic analysis of the upper left extremity and the lungs area showed that there was an irregular bone growth on the left caput humeri, and the lungs were normal. Based on the lesion, the dog was diagnosed as having a hyperthropic osteopathy (Marie Disease).
Isolation, Identification and Sensitivity Test of Staphylococcus aureus against Amoxicillin of the Milk sample in the Mastitis Crossbred Ettawa Goat at Girimulyo Area, Kulon Progo, Yogyakarta
No full textStaphylococcus aureus is the main bacteria causing mastitis of the Ettawa crossbreed goat. The mastitis causes significant economic losses due to decreasing of milk production. Mastitis in goat could threat the survival rate of her kids because decrease in quality and quantity of milk products. The toxin produced by S.aureus can also cause the death of their mothers. The present research is aimed to isolate and identify S. aureus from mastitis milk sample of Ettawa crossbreed goat, and does its sensitivity to amoxicillin as well. The milk samples were collected from the mastitis crossbreed Ettawa goat at Girimulyo area, Kulonprogo, Yogyakarta. In the present study, 20 milk samples of Ettawa crossbreed goat were used to isolate and identify S. aureus based on the culture, Gram staining, biochemical and sugar tests. The isolates then tested for their sensitivity to amoxicillin. Nine isolates in the present study identified as S. aureus in which they grew on MSA, formed round cells cluster, Gram +, fermented manitol, lactose and maltose. They coagulated the rabbit plasma and reacted positively to the clumping factor and Voges Proskouer test. Based on the results of the sensitivity test to 10 ìg amoxicillin is known that 8 isolates (88,89%) are sensitive to amoxicillin. Therefore, it can be concluded that there are 9 isolates from 20 mastitis milk samples of Ettawa crossbreed goat identified as S. aureus. Staphylococcus aureus isolates are mostly sensitive to amoxicillin.
The Effect of Various Thawing Temperatures of Frozen Semen on the Success of Artificial Insemination of Beef Cattle
Get PDFThe study about the effect of various thawing temperatures frozen semen on the success of artificial insemination of beef cattle had been conducted in Residency of Sleman Territory of Yogyakarta. The study used 59 heifers and cows that were oestrus (without oestrus stimulation) with body condition score (BCS) of 3 and 1,5-8 years age. They were inseminated using frozen semen of Simmental thawed in temperature of 37°C, 35°C (warm water) and 28-30°C (cold water). Effect of thawing temperatures was evaluated by non returnrate (NR). NR of thawing temperatures of 37°C, 35°C and 28-30°C were 63,16%, 55%, and 45%. Thawing temperature of 37°C increased the non returnrate compared to the thawing temperatures of 35°C and 28-30°C. Statistical analysis by Chi-square showed there were no differences between thawing temperatures of 37°C, 35°C and 28-30°C on the success of artificial inseminatio
The Influence of Kebar Grass Infuse to Mice (Mus musculus) Spermatogenesis
Get PDFThis study was aim to examine the effect of grass infusion kebar (Biophytum petersianum) to increase the activity of spermatogenesis in male mice. This research was conducted at the Laboratory of Botany University Discourse Kristen Duta Yogyakarta, Laboratory of Experimental Animal Care Unit and Unit III LPPT Gadjah Mada University in April 2009 to May 2009 using Swiss strain male mice aged 2.5 months as many as 25 tails, which are grouped in 5 groups treated with different concentrations, 0% (control), 1%, 3%, 5%, and 10% for 30 days, 2 times a day at a dose of 0.5 ml each time. Results of TLC method proves that these plants contain three types of chemical compounds that could potentially impact the process of spermatogenesis, ie, saponins, flavonoids and tannins. The analysis showed an increase in activity with increasing concentrations of spermatogenesis up to a concentration of 5% (spermatogonia: 226, primary spermatocytes: 227, secondary spermatocytes: 377, spermatids: 302) with a significant value of 0.432. Yet, at a concentration of 10% actually decreased spermatogenesis activity due to negative feedback. Conclusion: Infuse kebar grass proved to increase the activity of spermatogenesis, but at concentrations that are too high can cause a decrease in spermatogenesis activity
Prevalence of Toxoplasmosis in Sheep Slaughtered in Ngampilan Slaughterhouse Yogyakarta Using CATT Method
Get PDFThe research was conducted to find out the prevalence of toxoplasmosis in sheeps which were slaughtered in Ngampilan Slaughterhouse Yogyakarta using card agglutination test (CATT) method. Blood samples were collected from 50 sheeps. The blood samples were then centrifuged at 5000 rpm for 5 minutes in 4°C in order to obtain the sera of the sheeps. After that, each serum in the amounts of 10 µl was tested using CATT method. The positive CATT test resulted in the green background with red aggregate in the middle, whereas the negative CATT test resulted in the uniform brown discoloration. In conclusion, about 72% of sheeps slaughtered in Ngampilan Slaughterhouse Yogyakarta was positively infected toxoplasma. Toxoplasmosis prevalences for ewes, rams, sheeps and lambs are 79 %, 59 %, 72 % and 67 %, respectively.
Study of The Effects of Carboxymethyl Chitosan on The Non-specific Defense System in The Carp (Cyprinus Carpio)
Get PDFAbstract Carp (Cyprinus carpio) is a freshwater fish with a high economic value, but very susceptible to diseases. One of effort to increase the productivity is by enhancing non-specific defense system. The purpose of this study is to determine the effect of carboxymethyl chitosan on enhancement non-specific defense system of carp. Carboxymethyl chitosan was obtained by alkylation process in which monochloroacetic acid in alkaline conditions was added. Carboxymethyl chitosan was given to carps at dosages of 30 μg/g, 75 μg/g and 105 μg/g, by intra muscular injection respectively. Seven and 14 days after administration of carboxymethyl chitosan, measurements of non-specific immune system parameters were done. The results showed that, administration of carboxymethyl chitosan on carps affected the phagocytic activity and lymphocytes counts. However, carboxymethyl chitosan did not give any effect to NBT activity, hematocrit, number of erythtocytes and leukocytes, monocytes and neutrophil counts in blood as well.