Jurnal Sain Veteriner
Not a member yet
821 research outputs found
Sort by
Kajian Fenotip Kuskus (Famili Phalangeridae) di Penangkaran Desa Lumoli, Kecamatan Piru, Maluku
Get PDFKuskus merupakan satwa Australia (marsupial) yang termasuk dalam famili Phalangeridae danpersebarannya terbatas di Indonesia bagian Timur, Australia dan Papua New Guinea. Melalui data IUCN, kuskus dikategorikan endangered spesies, dalam CITES digolongkan Appendiks II. Populasi kuskus semakin menurun akibat ancaman deforestrasi, banyak diburu untuk dikonsumsi, dan diperjualbelikan secara ilegal. Untuk mengatasi persoalan diatas maka perlu dilakukan kajian komprehensif tentang fenotip kuskus dengan harapan dapat membantu usaha konservasi satwa ini di masa mendatang. Tujuan penelitian ini adalah mengetahui jenis serta sifat fenotip berdasarkan warna rambut kuskus yang hidup di penangkaran Desa Lumoli Kabupaten Seram Bagian Barat, Maluku. Penelitian ini diawali dengan tahap identifikasi dan karakterisasi setiap spesies secara fenotip melalui tampilan warna rambut. Karakter fenotip disajikan melalui gambar dan tabel, kemudian dianalisis secara deskriptif. Hasil penelitian karakterisasi fenotip menunjukkan terdapat empat spesies kuskus yang hidup di penangkaran desa Lumoli yakni, kuskus coklat (P.orientalis), kuskus putih (P.urinus), kuskus kelabu (P.vestitus), dan kuskus totol (S.maculatus)
Daya Hidup Spermatozoa Epididimis Kambing Peranakan Ettawa yang Dipreservasi dengan Pengencer Tris dan Bberbagai Konsentrasi Maltosa
Get PDFCauda epididymal spermatozoa could be used as an alternative source of gamete in the application of various reproductive technologies, since the spermatozoa is motile and has ability for fertilizing the oocyte. Theobjective of this research was to examine the effectivity of maltose in maintaining viability of ettawa crossbreed goat epididymal spermatozoa preserved at 3–5oC. Five testis with epididymides of ettawa crossbreed goat were obtained from slaughterhouse. Epididymal spermatozoa was collected by the combination of slicing, flushing and tissues pressure of cauda epididymides with physiological saline (0.9% NaCl). Collected-spermatozoa wasdivided in equal volume into three tubes and diluted with Tris extender containing 20% egg yolk (control), Tris extender + 0.3 g maltose/100 ml (M0.3), and Tris extender + 0.6 g maltose/100 ml (M0.6), respectively. Dilutedspermatozoa was stored in refrigerator at 3–5oC. Quality of diluted-spermatozoa including percentages of motile spermatozoa (MS) and live spermatozoa (LS) were evaluated every day during storage at 3–5oC for four days. Data were analyzed using completely randomized design with three treatments and five replicates. Means were compared significant difference test at 0.05 significant level. Results of this study showed that mean spermatozoaconcentration, percentage of MS, percentage of LS, and percentage of abnormal spermatozoa of ettawa crossbreed goat fresh epididymal spermatozoa were 3,220 million cell/ml, 70%, 81%, and 4.3%, respectively. At day-5 of storage, percentages of MS and LS for M0.3 (38 and 60.4%) and M0.6 (38 and 57.2%) were significantly (P<0.05) higher than control (32 and 55.4%). In conclusion, addition of 0.3 and 0.6% maltose in Tris extender could be maintained viability of ettawa crossbreed goat epididymal spermatozoa preserved at 3–5oC forthree days
Respons Imun Ayam Petelur Pascavaksinasi Newcastle Disease dan Egg Drop Syndrome
Get PDFSome viral diseases in poultry could lead to huge losses to the farmers. Newcastle Disease (ND) and Egg Drop Syndrome (EDS) are a group of infectious viral disease that can cause the decreasein egg production. Newcastle Disease is caused by Avian paramyxovirus type 1 (PMV-1) Paramyxoviridae family. The causative agent of EDS is Duck adenovirus-I Adenoviridae family. Both of these diseases affect the economic losses to the poultry. The main action to prevent hens from ND and EDS virus diseases is vaccination. The success ofvaccination can be tested by serology. ND and EDS virus characteristically agglutinate hen’s erythrocyte they have Hemagglutine protein on virus envelope so can be tested by hemagglutination. The study was conducted ona commercial poultry farm in order to determine the success of vaccination against ND and EDS. The hens were vaccinated by Newcastle Disease-Infectious Bronchitis- Egg Drop Syndrome (ND-IB-EDS) inactivated vaccines.Serological test was conducted in pre and post vaccination by using microtiter hemagglutination test. The antibody titre is expressed in units of HI log2. The results of the study, the mean antibody titer against ND pre vaccinationwas 4,53 ± 1,356 HI log2 and antibody titre in 2nd, 3rd and 4th week were 8,67 ± 0,617 HI log2, 7,73 ± 1,335 HI log2 and 5,20 ± 0,862 HI log2 post vaccination. Antibody titre against EDS pre vaccination was 0 ± 0,000 HI log2 and antibody titre post vaccination in 2nd, 3rd and 4th week were 7 ± 1,363 HI log2, 7,27 ± 1,438 HI log2 and 7,6 ± 1,056 HI log2. It showed that ND-IB-EDS inactivated vaccines is serological protective for ND and EDS titres
Suplementasi Calcitriol Menurunkan Risiko Osteoporosis Tikus Ovariektomi
Get PDFSuplemen calcitriol memicu pembentukan tulang, namun juga meningkatkan resorpsi tulang. Penelitian ini dilakukan untuk mengkaji efektivitas calcitriol dalam menurunkan risiko osteoporosis tikus ovariektomi. Lima belas tikus Wistar betina umur delapan minggu, dibagi tiga kelompok (kontrol normal KN, kontrol ovariektomi KOV, dan ovariektomi+ calcitriol OVD ) dan diberi perlakuan selama delapan minggu. Pada akhir perlakuan, 100 dilakukan pengambilan darah melalui pleksus retroorbitalis untuk pemeriksaan estradiol, selanjutnya semua tikus dietanasi menggunakan campuran ketamin10% dengan xylaxine 2%. Femur kiri diambil untuk pemeriksaan histopatologis. Hasil analisis menunjukkan tikus KOV mempunyai konsentrasi estradiol yang secara signifikan lebih rendah dibandingkan tikus KN, dan tikus OVD mempunyai konsentrasi estradiol tidak 100 berbeda signifikan dibanding tikus KOV. Gambaran histopatologis epifisis tulang femur distalis tikus OVD 100terlihat mempunyai lebih sedikit jaringan adiposit dan lebih banyak spikulum trabekula dibanding tikus KOV. Dari hasil penelitian disimpulkan bahwa suplementasi calcitriol 100ng/hari selama delapan minggu pada tikus ovariektomi menurunkan risiko osteoporosis
Karakterisasi Antibodi Poliklonal terhadap Aflatoksin M1
Get PDFThe aim of the research was characterized the polyclonal antibody at AFM for diagnostic reagens on track 1 and measure the levels of AFM in milk. The series of experiments are given a polyclonal antibody testing 1 dimetilaminobenzidin (DAB) by dot blot immunoassay (DBIA) so it will look brown color display on the positive control sample; purification and estimation antibody of AFM concentration with ammonium sulfate; 1 dialysis; fractionation using HiTrap Protein A HP column, measured using a spectrophotometer at a wavelength of 280 nm; and characterized of IgG using SDS-PAGE. DBIA test results showed a typical reaction betweenantigen AFM1-BSA with AFM1-BSA antibody to rabbit serum in the form of brown dots after addition of DAB substrate. The results of spectrophotometric against rabbit serum fractionation showed the type of IgG heavy chain
Detection of Edwardsiella tarda From African Catfish (Clarias garipienus) by Agar Gel Precipitation (AGP) Method in jambi
Get PDFFor the past few years, Edwardsiella tarda has become major problem in African catfish culture in Jambi. Detection by biochemical characteristic can lead to inaccurate result and there is a necessity to develop more specific and accurate method, one of which is Agar Gel Precipitation (AGP) method. Six samples each were collected from two African catfish farm located in District Sungai Gelam and Telanai Pura in Jambi, which was showed clinical signs of E. tarda outbreak with more than fifty percent mortality rate. Heat stable soluble antigen was prepared from 2 groups of pure culture isolated from sample for AGP test. Antiserum for test wells was antiserum of E. tarda (ATCC 15947) that have been produced by inoculating whole-cell antigen (heat-stable) and flagellar antigen (formalin-killed) in rabbit. For control positive, soluble antigen prepared from E. tarda (ATCC 15947), and control negative from Aeromonas hydophila (ATCC 35654) and Edwardsiella ictaluri (NCIMB 13272). Both antiserums were able to show positive reaction visible by the formation of specific precipitin lines between antiserum and antigen wells, and there was no precipitin reaction for negative control. In conclusion AGP method is a one of reliable technique to identify E. tarda
Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer
Get PDFTrimethyltin chloride (TMT) is an organotin compound which neurotoxic at limbus system and hippocampus in human and animal. Pathology changes that caused by the induction of TMT is a neurodegenerative disorder such as nerve cell death and cognitive impairment. This study was aimed to observe brain pathology induced by TMT with multiple doses for 14, 21 and 28 days after treatment. Twenty seven of Wistar rats, at 2 months of age with weight ranging between 200-300 grams were used and divided randomly into 3 groups (n=9). Group I were injected by trimetyiltin with a dose of 6 mg / kg, group II were injected bytrimetyltin with a dose of 8 mg / kg and group III as control without injection. Observation of brain pathology was done by euthanasia on day 14, 21 and 28 after treatment, three rats each. Cortex and hippocampus of the brainwere observed using Hematoxilin and Eosin staining (HE). All of the research procedure was done with the approval and supervision of Animal Ethics Committee LPPT UGM No. 300/KEC-LPPT/VII/2015. The observation of histopathology of the brain's neuron cells injected by trimetyltin dose of 6 mg/kg and 8 mg/kg body weight was showed increasing cell death of brain neurons in the cortex and hippocampus compared to the control group. The highest cell death was on day 14 in the hippocampus and cortex cerebral on day 21after TMT injection. The neuron cell death characterized by the shrink of brain neurons as well as colored eosinophilic cytoplasm. One way ANOVA statistical analysis showed a significant difference number of neurons cell deathbetween control and treatment groups. Based on this research, it can be concluded that the trimetyltin injection dose of 6 mg / kg and 8 mg / kg of body weight caused neuron cell death in the brain rats from fourteen day aftertreatment, especially in the hippocampus and cortex
Keberadaan Anisakis typica (Anisakidae) dari Ikan Tongkol dan Ikan Layang dari perairan Sulawesi Barat
Get PDFAnisakis (L3-stage) is a parasitic nematode commonly found in marine fish or squid serve as intermediate or paratenic host. The purpose of this research was to identify Anisakis larvae using PCR-RFLP and sequencing methods. A total of 30 individuals of each species: bullet tuna (Auxis rochei) and Indian scad(Decapterus russeli) were examined for presence of Anisakis spp. Fish samples caught by fishermen around Mamuju waters were purchased at a fish market. The fish was necropsied and the organs: liver, intestine wall, heart and muscle were removed and put on separated petri dishes and examined for parasites under a stereo microscope. Anisakis found was cleaned and fixed in 70% alcohol. Initial identification was based on ventriculus shape and presence of mucron to distinguish Anisakis type I and Anisakis type II. All Anisakis found belong to Anisakis type I. Ten Anisakis type I were isolated, cleaned and stored in microfuge tubes containing 70% alcohol. The parasite gDNA was extracted using Wizard genomic DNA extraction and purification kit (Promega).Species of Anisakis was determined by PCR-RFLP at ITS1-5.8S-ITS2 region using two restriction enzymes: Taq I and Hinf I and PCR-sequencing in mtDNA cox2 region. Analysis by PCR-RFLP showed that all Anisakis type Iexamined was in the same pattern as Anisakis typica. Sequencing in mtDNAcox-2 region and the phylogenetic analysis showed that all samples were in the same cluster as A. typica. Based on PCR-RFLP and sequencinganalysis, all Anisakis found in this study belong to A. typica
Kerusakan Usus pada Mencit (Mus musculus) yang Diinokulasi Larva 3 (L3) Anisakis spp.
Get PDFThis study aims to be show damage in the intestine of mice caused by inoculation of the larvae of Anisakis spp. alive and who has died because of a warming 750C for 7 minutes. Eighteen male mice (Mus musculus) were divided into three groups: control group, a group of mice were inoculated the larvae of Anisakis spp. alive andgroups of mice were inoculated the larvae of Anisakis spp. who have died. 48 hours post inoculation, the mice’s intestines necropsy performed later performed HE staining to identify and scoring intestinal histopathology. The results showed inoculation of the larvae of Anisakis spp. either alive or dead induce histological changes in the intestine in the form of infiltration of inflammatory cells, epithelial changes and structural changes in the intestinal mucos
Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani
No full textGlucose transporter (GLUT 4) is glucose transporter protein regulated by insulin, found in adipose tissue and striated muscle (skeletal and cardiac muscle). Kedu cemani chicken is one of Indonesia endemic animal, found in Kedu, Temanggung regency, Central Java. This study was required to complete microscopic documentation of Indonesia’s native biodiversity. The objective of this study was to clarify GLUT 4 distribution in skeletal muscle fibers of kedu cemani chicken by using avidin-biotin-peroxidase complex (ABC) immunohistochemistry method. This study was conducted by using pectorales major, biceps brachii, and biceps femoris muscle tissue from 5 kedu cemani chicken. The result showed that GLUT 4 immunoreactivity were detected in sarcolemma and myofibrils component of pectorales major, biceps brachii, and biceps femoris muscle tissue. Intensity of GLUT 4 immunorectivites increased from weak intensity in pectorales major muscle tissue, moderate intensity in biceps brachii muscle tissue, then strong intensity in biceps femoris muscle tissue. This result might motivate to further exploration about the other kedu cemani chicken specific features to complete microscopic documentation of Indonesia’s native biodiversity.