Jurnal Sain Veteriner
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Deteksi dan Identifikasi Cemaran Virus Avian Influenza pada Pasar Tradisional di Kabupaten Aceh Besar dan Kota Banda Aceh
Get PDFPenelitian ini dirancang untuk mendeteksi keberadaan virus AI pada beberapa titik kritis, yaitu unggas, kandang penampungan sementara, tempat pemotongan dan penjualan karkas, dan mengidentifikasi subtipe virus AI pada beberapa pasar tradisional di Kabupaten Aceh Besar dan Kota Banda Aceh, Propinsi Aceh. Sampel swab diambil secara acak (random) dari empat pasar di Kabupaten Aceh Besar dan Kota Banda Aceh, Propinsi Aceh, yaitu pasar Lambaro, Ketapang, Ulekareng dan Peunayong, dan dipoolkan. Satu pool sampel berisi 1 sampai 5 swab yang dikelompokkan berdasarkan pedagang, jenis unggas, dan titik kritis cemaran AI. Jumlahtotal sampel yang diambil adalah 314 swab, yang terdiri dari swab trakea unggas hidup, kandang penampungan, meja tempat pemotongan, dan karkas, kemudian dikelompokkan dalam 121 pool. Isolasi virus AI kedalam telur ayam bertunas (TAB), pemeriksaan secara serologis (uji HA/HI), dan identifikasi molekuler (metode RT-PCR) di lakukan di bagian Virologi Laboratorium Veteriner, dinas Kesehatan Hewan dan Peternakan Provinsi Aceh, dan Laboratorium Riset Terpadu Fakultas Kedokteran Hewan Universitas Syiah Kuala Banda Aceh. Analisis hasil pemeriksaan serologis dan identifikasi molekuler virus AI dilakukan dengan metode deskriptif. Hasilisolasi virus pada TAB menunjukkan bahwa embrio mengalami kematian pada 1 hari setelah di infeksikan dengan material virus dari sampel swab yang berasal dari pasar Lambaro Kabupaten Aceh Besar, dan titer antibodi terhadap virus AI hanya terdeteksi pada itik dan ayam pedaging yang berkisar antara 24 sampai dengan 27. Dari hasil serologis tersebut dilanjutkan dengan pemeriksaan RT-PCR. Berdasarkan hasil uji RT-PCR menggunakan primer spesifik terhadap gen M, H5, dan N1 diperoleh virus AI yang bersirkulasi pada unggas di pasar Lambaro tergolong subtipe H5N1. Hasil penelitian ini membuktikan bahwa diantara beberapa titik kritiscemaran virus AI di pasar tradisional, unggas hidup (itik dan ayam pedaging) yang berada dalam kandang penampungan sementara merupakan potensial penyebaran virus AI ke lingkungannya
Deteksi Edwardsiella Ictaluri pada Ikan dengan Metode Co-Agglutination Test
Get PDFTeknik immunodiagnostik dengan menggunakan uji serologis co-agglutination telah lama dikenal dalam mendeteksi infeksi bakteri dan virus pada manusia, hewan, ikan, dan cemaran pada produk-produk perikanan. Keunggulan teknik ini adalah sederhana, cepat, murah, dan akurat, terutama untuk penerapan di lapangan karena dapat mendeteksi langsung dari organ. Namun penggunaan metode kurang dikenal dibandingkan metode lain yang lebih kompleks dan mahal seperti ELISA dan FAT. Pengembangan metode ini di Indonesia telah dilakukan sejak tahun 2014 dan telah diaplikasikan di lapangan dengan hasil yang memuaskan, terutama dalam mendeteksibakteri Edwardsiella ictaluri penyebab penyakit Edwardsiella Septicemia of Catfish pada ikan patin (Pengasius hypophtalmus) dan lele (Clarias garipienus) di Jambi. Pengambilan sampel dilakukan di 70 lokasi sentra budidaya ikan patin dan lele di 4 kabupaten, di Provinsi Jambi selama bulan April-Juni 2015. Pengujian dengan teknik co-agglutination mendeteksi 5 lokasi budidaya positif tersebut E. ictaluri. Organ yang diuji dengan teknik ini diisolasi ke media agar untuk diuji secara konvensional dan serologis dengan metode rapid slide aglutination dan terkonfirmasi positif. Isolat yang diperoleh juga diujibandingkan ke laboratorium yang telah terakreditasi dalam ruang lingkup E. ictaluri di Batam dan Tanjung Pinang, dan mendapatkan hasil positif. Uji lapangan inimembuktikan bahwa teknik co-agglutination dapat memberikan hasil yang valid dalam waktu beberapa jam saja dibandingkan dengan metode konvensional yang memakan waktu berhari-hari, ataupun dengan metode ELISA dan FAT yang membutuhkan biaya tinggi. Inovasi teknologi dengan metode ini dapat menjadi jawaban atas tantangan globalisasi terutama dengan diberlakukannya kawasan Masyarakat Ekonomi Asean, dimana lalulintas peredaran ikan dan pengembangan industri perikanan makin meluas yang membawa resiko merebaknya wabahpenyakit ikan
Efek Ekstrak Daun Binahong (Anredera cordifolia (Ten) Steenis) terhadap Profil Darah Merah pada Marmut (Cavia cobaya)
Get PDFThe purpose of this study was to determine the effect of binahong leaves extract (BLE) on Cavia cobaya through the number of erythrocytes, hemoglobin, pack cell volume (PCV) and erythrocyte indices. Complete Random Design (CRD) was used in this study with 4 treatment and 4 replicate, those were T0 (control), T1 (10 mg / head / body weight), T2 (50 mg / head / body weight) and T3 (90 mg / head / weight). The administration of BLE was orally for 10 days pre partum and 10 days postpartum. Blood sampling was done three times, which wasbefore giving BLE , 1 day postpartum and 1 day after the giving of BLE. Data was analyzed using ANOVA and if there was effect of treatment, then continued with Duncan multiple range test. There was not significantly different among treatments for PCV before giving BLE, while the postpartum and post giving BLE showed significantly different (P<0.05). There was not significantl different in the percentage of Hb among treatments for postpartum while in before giving BLE and post giving BLE were showed significantly different (P<0.05). Total erythrocytes was not significantly difference among treatments for before giving BLE while in the postpartum and post giving BLE significantly different (P <0.05). MCHC value was not significantly different between treatments which was before giving BLE and post giving BLE while in the postpartum was showedsignificantly different (P<0.05). MCV and MCH values were not significantly different between treatments before giving BLE while in the postpartum and post giving BLE significantly different (P <0.05). In conclusion, giving BLE at a dose of 50 mg/head/body weight can improve immunity and endurance Cavia cobaya on the treatment group before giving BLE, postpartum and post giving BLE of PCV, Hb, total erythrocytes, MCHC, MCV and MCH
Pengendalian Folikulogenesis Ovarium dengan Pemberian Ekstrak Biji Kapas
Get PDFGosipol is a substances contained in extracted cotton seed which is thought to have the antifertility ability therefore it is often used as a herbal contraceptive. The aim of this study were to assess the folliculogenesis in mice after administrated with cottonseed extract. 60 female mice strain DDY which was 14-15 weeks old and 30-35 g body weight were divided into five groups and given cottonseed extract each 0; 1,5; 2,1 and 2,7 g/kg BW for 5, 10, 15, 24, and 24 + 10 days (without cottonseed treatment). At the end of the treatment period, mice was euthanasia to observe follicular development histomorphology (each three mice of each treatment). Mice estrous status were evaluated based on the description of the vaginal smear cells with Giemsa staining. The results showedthat the number of developing follicles was low (P < 0.05) compared with control after 5 days cottonseed extract administration at dose 2,7 g/kg BW that were 23 ± 3,6. At dose 1,5 and 2,1 g/kg BW the number of follicles was low after 24 days that were 25 ± 10,4 and 27 ± 3,5. Recovery effects of follicle number after cottonseed extract administration for 24 days was the best at a dose of 1,5 g/kg BW. Prolonge of estrous cycle occured in mice which were administrated the cottonseed extract of at all dose treatment. In conclusion, although the decrease in the number of developing follicles and prolonge of estrous cycles occurred after cottonseed extract administration, but these effects are reversible after the administration ended
Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani dengan Metode Imunohistokimia Avidin-Biotin-Peroxidase Complex
No full textGlucose Transporter 4 (GLUT 4) merupakan protein transporter glukosa yang diatur oleh insulin, ditemukan terutama di jaringan adiposa dan otot lurik (baik otot skelet maupun otot jantung). Ayam kedu cemani merupakan ayam endemik Indonesia yang terdapat di wilayah Kedu, Temanggung, Jawa Tengah. Penelitian ini diperlukan untuk melengkapi dokumentasi data anatomi mikroskopik kekayaan hayati asli Indonesia. Penelitian ini bertujuan untuk mengklarifikasi distribusi GLUT 4 dalam serabut otot skelet ayam kedu dengan metode imunohistokimia avidin-biotin-peroxidase complex (ABC). Penelitian dilakukan dengan menggunakan jaringan otot yang meliputi otot pectorales mayor, biceps brachii, dan biceps femoris dari 5 ekor ayam kedu cemani. Hasil penelitian menunjukkan bahwa immunoreaktivitas GLUT 4 terutama terdeteksi di sarkolema dan komponen miofibril pada otot pectorales mayor, biceps brachii, maupun biceps femoris. Intensitas imunorekatifitasnya meningkat dari intensitas lemah pada otot pectorales mayor, menjadi intensitas sedang pada otot biceps brachii, dan kemudian intensitas kuat pada otot biceps femoris. Hasil penelitian memunculkan dorongan untuk menggali lebih lanjut ciri-ciri khusus ayam kedu cemani lainnya, untuk mendokumentasi data anatomis kekayaan hayati asli Indonesia
Efek Ekstrak Air Biji Pepaya (Carica papaya L.) terhadap Fertilitas Mencit (Mus musculus L.) Betina
No full textPapaya seeds were known as medicinal plant which have anti-fertility potencies. So it could be used as a herbal contraceptive. The purpose of this study was to test the anti-fertility effect of Papaya seed water extract on mice (Mus musculus L.) fertility which proved by pregnancy rate, litters number and sex ratio of litters. This study was designed by Completely Randomized Design, using 15 female Swiss Webster mice that were divided into 5 treatment groups with triplicates. The K(-) group were treated by distilled water; K(+) group were treated by contraceptive pills; P1, P2, P3 groups that were treated by papaya seeds extract with 1.4, 3.5 and 7 mg/animal/day respectively. Each treatment was given orally with 0.5 ml in volume for 21 days. At the end of treatment, mice was mated. The observed parameters were pregnancies occurence, litters number and sex ratio of the litters. The litters number data were analyzed by Analysis of Variance. The results showed there was differences in pregnancy time and sex ratio between control and treatment groups, but there was not significantly different in litters number. It could be concluded that the fertility of mice was inihibited by water extract of papaya seeds
Karakteristik Fisik dan Kimia Telur Burung Mamoa (Eulipoa Wallacei) di Pantai Uwo Uwo Kecamatan Galela Kabupaten Halmahera Utara
No full textThe aim of this research was to determine physicochemical characteristics of Mamoa egg. The eggs were collected about 100 eggs from Uwo uwo beach. Physical quality was observe in Animal Husbandry laboratory, Faculty of Agricultural, Khairun University. The physical characteristics observed were measuring include weight, shape, colour of eggshell, length, diameter, egg index, albumen, yolk, weight of eggshell, haugh unit, and thickness of eggshell. The chemical characteristics consist of moisture content, protein, fat content, fiber content, ash, carbohydrate, cholesterol, and antioxidant. Data were analyzed by quantitative descriptive analysis. The result showed that physical quality of egg were weight = 97.28 g, length = 7.12 cm, diameter = 5.45 cm, index of egg = 71.89%, Percentage of yolk and albumen were 69.12% and 23.24%, haugh unit score = 68.78 and shape of egg was ovale. The chemical characteristic of egg chemical quality were protein content = 15.47%, fat content = 8.31%, carbohydrate = 25.52 %, cholesterol = 387.54 mg/100gr, and antioxidant = 49.08% dpph
Penyakit Ginjal Kronis pada Anjing dan Kucing: Manajemen Terapi dan Diet
Get PDFChronic kidney disease is a progressive impairment of renal function and irreversible. The kidneys fail to maintain metabolism and fluid and electrolyte balance, causing uremia. This disease is a common problem in old cats and dogs that not detected by the owners up to 75 % of kidney function is damaged. Clinical signs vary as polyuria, polidipsi, anorexia, vomiting, weight loss, pale mucous membranes, mouth ulceration, halitosis and acute blindness. Chronic renal failure is not curable so that the necessary medication management and proper diet in orderto improve the quality of life and prolong the life of the animal
Morfologi Hemipenis Biawak Air (Varanus salvator bivittatus)
Get PDFVaranus salvator bivittatus has a pair of hemipenes, which is macroscopic anatomically like the copulatory organ in other amniotes, cylindrical-shaped (truncus) with quite flexible, and it located on the base of the caudal tailof the cloaca. However, information about the microscopic anatomy of hemipenes of this animal is scientifically unpublicized. Therefore, the aim of this research is to study the microscopic anatomy of hemipenes of male Varanussalvator bivittatus. The animals were sacrificed by exsanguination under deep anesthetized and fixed in 4 % paraformaldehyde through perfusion then observed visceral site and morphometric. Histomorphological evaluationwas obtained by paraffin preparation with section thickness of 3-4 μm then stained in Hematoxylin-Eosin (HE) and Masson’s Trichrome (MT). The results showed that truncus of hemipenes was lined by stratified squamousepithelium and supported with thick of dense connective tissue and contain cavernous body and blood vessels are found, the muscles not found. The presence of connective tissue that supported in the down part sometimesmake hemipenes are rigid while prurient condition. In the caudal of truncus hemipenes there is retractor muscle of hemipenes which arranged by striated muscles. Hemipenes is flexible because contain with much of blood vesselthat found in truncus hemipenes