Jurnal Sain Veteriner
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Performa Produksi dan Analisis Usaha Puyuh (Coturnix coturnix japonica) yang diberi Substitusi Black Soldier Fly Larvae (BSFL) pada Pakan Komersil
Get PDFBlack Soldier Fly Larvae (BSFL) is an alternative protein source for livestock. This research aimed to know the effectiveness of BSFL for commercial feed substitution, related to production, productivity, and profitability in quail farm. This study used 80 quails which divided into 4 groups and each group consist of 20 quails. The treatments contained BSFL substitution with different composition, control group (P0) was given 100% commercial feed, (P1) was given a 5% BSFL substitution, (P2) was given a 10% BSFL substitution and (P3) was given a 20% BSFL substitution. Contribution margin (CM) analysis was used to determine the yields of the groups in this study. Results showed that the highest feed consumption rate is in P3, production (egg in total and weight), productivity and yields in P3 is the highest, although there's no difference between P0, P2 and P3 based on the analysis MANOVA. BSFL substitution at 20% is recommended to the farmers to increase profits
INDENTIFY OF SEROVAR THAT CAUSED CANINE LEPTOSPIROSIS IN YOGYAKARTA
No full textAbstractLeptospirosis is a zoonotic disease of global concern, and is caused by pathogenic serovar Leptospire interrogans. Canine Leptospirososis is widespread throughout the world, dogs can act as incidental hosts or maintenance hosts for various serovars. The purpose of this reaserch was to identify leptospire serovars that infect healthy and suspected leptospirosis dogs in Yogyakarta. A total of 56 dogs (36 healthy dogs and 20 suspect leptospirosis dogs) were done blood collection from cephalica vein 3 ml, serum was separated for leptospirosis examination with Microscopic Aglutination Test (MAT) which conducted at the Research Center for Veterinary Science, Bogor. Microscopic Aglutination Test carried out on various Leptospire serovar, namely: Ichterohaemorrhagiae, Javanica, Celledoni, Ballum, Pyogenes, Cynopeteri, Rachmati, Auatralis, Pomona, Canicola, Grippotyphosa, Bataviae, Hardjo, and Tarrasovi. The results showed that Celledoni serovars infected 25% of healthy dogs and 5% of suspect leptospirosis dogs, Javanica serovar infected 19% of healthy dogs, Bataviae serovars infected 15% of suspect leptospirosis dogs, Grippotyphosa serovar infected 11% of healthy dogs, Tarrasovi serovar infected 10% of suspect leptospirosis dogs, serovars Cynopteri infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, serovar Pyrogenes infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, and serovar Rachmati infects 5% of suspect leptospirosis dogs. Seven healthy dogs (19%) and 2 suspect leptospirosis dogs (10%) were infected with more than 2 leptospire serovars. From the results of this study it can be concluded that Celledoni serovar of Leptospira interrogans infection causes subclinical leptospirosis, while Bataviae serovar infection causes clinical leptospirosis in dogs in Yogyakarta. Abstrak Leptospirosis adalah penyakit zoonosis yang menjadi perhatian global, dan disebabkan oleh serovar patogen Leptospira interrogans. Leptospirososis pada anjing tersebar luas di seluruh dunia, anjing dapat berperan sebagai incidental host ataupun maintenance hosts untuk berbagai serovar. Tujuan penelitian ini untuk mengidentifikasi serovar leptospira yang menginfeksi anjing sehat maupun anjing terduga leptospirosis di Yogyakarta. Penelitian ini menggunakan serum yang diambil dari 56 ekor anjing di Yogyakarta, yang terdiri dari 36 anjing tanpa gejala leptospirosis (sehat) dan 20 anjing dengan gejala leptospirosis (sakit). Serum dikoleksi untuk pemeriksaan Microscopic Agglutination Test (MAT) yang dilaksanakan di Balai Besar Penelitian Veteriner (BBLitvet), Bogor. Uji dilakukan terhadap 14 serovar Leptospira, yaitu: Ichterohaemorrhagiae, Javanica, Celledoni, Ballum, Pyogenes, Cynopeteri, Rachmati, Australis, Pomona, Canicola, Grippotyphosa, Bataviae, Hardjo, dan Tarrasovi. Hasil penelitian menunjukkan bahwa serovar Celledoni menginfeksi 25% anjing sehat dan 5% anjing sakit, serovar Javanica meninfeksi 19% anjing sehat, serovar Bataviae menginfeksi 15% anjing sakit, serovar Grippotyphosa menginfeksi 11% anjing sehat, serovar Tarrasovi menginfeksi 10% anjing sakit, serovar Cynopteri menginfeksi 5% anjing sehat dan 5% anjing sakit, serovar Pyrogenes menginfeksi 5% anjing sehat dan 5% anjing sakit, dan serovar Rachmati menginfeksi 5% anjing sakit. Tujuh ekor anjing sehat (19%) dan dua ekor anjing sakit (10%) terinfeksi lebih dari dua serovar leptospira. Dari hasil penelitian ini dapat disimpulkan bahwa infeksi Leptospira interrogans serovar Celledoni menyebabkan terjadinya leptospirosis subklinis, sedangkan infeksi serovar Bataviae menyebabkan leptospirosis klinis pada anjing-anjing di Yogyakarta.
PRIMA KRIM SEBAGAI SOLUSI PENYEMBUH LUKA PADA PENDERITA DIABETES MELITUS
No full textJumlah penderita diabetes melitus sangat besar, menurut International Diabetes Federation (IDF) pada tahun 2013 mencapai 382 milyar orang dengan usia antara 40-59 tahun. Luka diabetik dikarakteristikkan sebagai luka kronis yang memiliki waktu penyembuhan yang lama. Limbah darah di Rumah Potong Hewan selama ini masih belum termanfaatkan, darah mengandung PRP (Platelet Rich Plasma) yang mampu mempercepat penyembuhan tulang dan jaringan lunak. Pembuatan PRP (Platelet Rich Plasma) dari darah sapi dilakukan dengan metode sentrifus. Tujuan penelitian adalah membuktikan bahwa Prima Krim mampu menyembuhkan luka pada penderita Diabetes Melitus. Hewan coba yang digunakan dalam penelitian ini adalah 20 ekor tikus galur wistar. Induksi diabetes dengan menggunakan streptozootocin 65 mg/kg dan nicotinamide 235 mg/kg. Dibagi menjadi 4 kelompok yaitu non diabetes diberi placebo (ND-), diabetes diberi placebo (DM-), diabetes diberi obat komersil yang mengandung kloramfenikol basa 20 mg + prednisolon 2,5 mg (DM+) dan diabetes diberi krim PRP dengan konsentrasi 20% (DM PRP). Parameter yang dipakai untuk mengetahui tingkat kesembuhan luka adalah diameter luka dan pemeriksaan histologi. Analisis data dengan Anova One Way pada derajat signifikansi 95%. Kelompok DM PRP memiliki tingkat kesembuhan luka yang lebih baik daripada kelompok ND-, DM- dan DM+. Berdasarkan pembacaan preparat histologi melalui skoring hasil meliputi epitelialisasi, membrana basalis, fibroblast, jaringan kolagen, dan ada tidaknya sel radang, didapatkan hasil kesembuhan terbaik ditunjukkan pada DM PRP. Krim PRP 20% yang dihasilkan dari limbah darah sapi mampu memberikan kesembuhan luka pada penderita diabetes melitus
Fucoidan : Manfaat dalam Industri Peternakan
No full textResearch of interest marine algae and seagrasses have been renewed, because they are considered to be promising resources of functional ingredients in the development of novel products. Fucoidans are a class of fucose-rich sulfated carbohydrates found in brown marine algae and echinoderms, and more recently identified in seagrasses. Chemical compositions of most fucoidans are complex, mainly being composed of fucose and sulfate. They also contain other monosaccharides like mannose, galactose, glucose, xylose and uronic acids, even acetyl groups and protein and it’s have potential therapeutic properties, including anti-viral activity. Currently, fucoidans are available for use in therapeutic and dietary supplements for inclusion in pet, livestock and aquaculture feed supplements. This review summarizes experimental evidence indicating that Fucoidan can improve the quality of feed ingredients for livestock, pets and aquaculture to improve health
Deteksi Kejadian Residu Tetrasiklin pada Daging Ikan Nila di Kota Yogyakarta dengan Kromatografi Cair Kinerja Tinggi (KCKT)
Get PDFFood products of animal origin which are free from biological and chemical contamination are an absolute requirement of food safety. Antibiotics residues in the food of animal origin is one of the chemical contaminants in food are harmful/hazardous to human health. Tetracycline is an antibiotic that is often used in the fishing industry. The study aims to detect the occurrence of tetracycline residues in tilapia sold in traditional markets in Kota Yogyakarta. The research was conducted with detection test (detect disease). The study used 61 samples of tilapia fish from 16 traditional markets in Kota Yogyakarta. Tilapia meat samples are prepared according to the Association of Official Analytical Chemistry (AOAC). The existence of tetracycline residues in meat can be detected by high performance liquid chromatography (HPLC). Residue analysis in fish meat was conducted at the Department of Pharmacology, Faculty of Veterinary Medicine of Gadjah Mada University using HPLC Shimadzu version 6.1. The results showed that 24 samples of tilapia meat marketed in Kota Yogyakarta was positively contained tetracycline antibiotic residues. A total of 19 samples contained residues above maximum residue limits (MRL). The conclusion of the study is 31% of tilapia marketed in Kota Yogyakarta contains tetracycline residues above MRL. Surveillance of residues in food of animal origin and further research in terms of reducing antibiotic residue in tilapia meat is highly recommended to improve the quality and safety of food of animal origin
Pengaruh Infusa Biji Buah Pinang (Areca catechu) Terhadap Tingkat Kematian dan Morfometri Cacing Dewasa Ascaridia galli Secara In Vitro
No full textThe study evaluates in vitro effects of Areca catechu crude aqueous extract as anthelmintics on Ascaridia galli adult mortality rate and morphometry. Naturally infected chickens were collected from slaughtered chickens at local slaughterhouses in Yogyakarta. Chicken’s intestines were carefully examined and transported to the Parasitological Laboratory of Gadjah Mada University, Yogyakarta. Ascaridia galli were collected from intestines (including duodenum, jejunum, and ileum) and put into a petri dish containing 0.62% saline water. There were 60 A. galli in each concentration of Areca catechu crude aqueous extract. Investigation on mortality rate of adult worms at various concentration and observation as well as differences in A. galli morphometry and mortality rate were analyzed using analysis Anova. The figures of the parasites or the parts of the parasites were captured using camera Lucida and then they were measured using both micrometer and curvimeter. The morphology of the parasites was identified to find out their morphometric characteristics.The results of morphometric observation of the A. galli showed that there was a significant difference in the body width and the vulva length of the females and in the body width, the esophageal length and the esophageal width of the males.This research disclosed that Areca catechu crude aqueous extract significantly affected the mortality rate of adult A.galli at various concentration. This research observed that the best concentration to kill the adult worms was 25% Areca catechu crude aqueous extract
Studi In-Vivo Ekstrak Daun Teh Hijau (Camellia Sinensis) sebagai Alternatif anti Bakteri Eschericia Coli pada Ayam Broiler
No full textThe prevalence of colibasillosis in chicken farms in Indonesia is very high, treatment using antibiotics is experiencing resistance, so it is necessary to look for alternatives to antibacterial. The study was aimed to determine the antibacterial effect of green tea leaf extract on broiler chickens infected with Eschericia coli by looking at the score of macroscopic lesions strengthened by histopathological examination, heterophile examination, plasma protein and fibrinogen. The research used 20 day old broilers (DOC) which were randomly divided into 4 groups, group A group B, group C and group D, each consisting of 5 DOC broilers. While maintaining ND and Gumboro vaccines on schedule like maintenance in general. At the age of 21 days all broilers in each group began to be treated as controls (Group A) without infecting E. coli and were not given 0,1g/ml water extract of green tea leaves (Camillia sinensis). Group B, intratracheal-infected broilers with local strains of E.coli were 108 cells / ml according to 0,5 Mc Farland standard, and were not given green tea leaf extract. Group C, broilers infected by intratracheal with local strains of E. coli 108 cells / ml by 0,5 Mc Farland standard, and given to drink green tea leaf extract (Camillia sinensis) 0,1 g/ml and group D, broilers were given drinking green tea leaf extract (Camillia sinensis) 0,1g/ml. During the treatment all of chickens were given food and drink ad libitum. Fourteen days after infection of E.coli, 5 chickens in each group were collected to collect blood for heterophyll, total plasma protein (TPP) and fibrinogen. And then were euthanasied with Mg SO4 saturated solution intravenously injection and necropsied for gross and histpathological examination. Analysis of blood tests results were used one way of anova (SPSS version 22 program), whereas for gross and histopathological examination with descriptive analysis. The results showed that the gross examination and histopathological organs of brolier infected with E. coli without being given a green tea extract experienced airsacculitis, pericarditis, perihepatitis and peritonitis, whereas broilers infected with E. coli and given green tea extract does not indicate the presence of inflammation. Examination of heterophile counts and blood fibrinogen levels had shown a difference (P 0.05). The conclusion can be drawn, that the study of in vivo green tea extract (Camelia sinensis) 0,1g/ml has the potential to inhibit the infection of Eschericia coli bacteria in broiler chickens
Studi Reseptor Virus Infectious Bursal Disease (IBD) pada Organ Limfoid Ayam Pasca Vaksinasi dengan Metode Imunohistokimia
No full textInfectious bursal disease (IBD) affects economical impact for breeders due to it can cause damage lymphoid organ, especially bursa of Fabricius and causing failure the vaccination program. Infectious bursal disease virus receptors on lymphoid organs suspected contribute to subclinical and clinical IBD incidence in the chickens. The aim of this study was to determine and compare the distribution of IBDvirus receptors on lymphoid organ of the chicken which obtained different IBD vaccination program. The presence of virus receptors in bursa of Fabricius, spleen, and thymus were observed microscopically using immunohistochemical method and evaluated with Image J® software. Monoclonal anti LSCC-BK3 (Gifu University, Japan) antibodies as primary antibody was used in this study. The result showed that IBDvirus receptors found abudantly in the bursa of Fabricius, afterwards in the spleen and thymus. No significant differences of IBD virus receptor distribution within lymphoid organs between chicken which obtained once and twice IBD vaccination. Infectious bursal disease virus receptor distribution in bursa Fabricius chickens aged 23 days received twice IBD vaccination more than once IBD vaccination program
Kertas Saring sebagai Media Transpor Darah untuk Pemeriksaan Antibodi Rabies
Get PDFRabies is a zoonotic disease. Rabies protection level detection was performed using antibody titration. Blood sampling activities in the field require special handling to avoid blood lysis, the sample delivery requires a cold chain with a stable temperature. Alternative method forwhole blood sample delivery using filter paper were carried out on 48 samples from susceptible animals (dogs and cats) transpored through the Soekarno Hatta Agricultural Quarantine Center. This study was designed to investigate the feasibility of filter paper sampling of blood at temperature of 26 °C to detect of rabies antibodies using indirect method of ELISA. The results of statistical analysis of Randomized Block Design and Tukey's test showed that the antibody titres of whole blood extracted from filter paper diluted in the PBS T 100 μl were equivalent to antibody titres of serum. Assesment of filter paper capability has sensitivity and specificity as much as 96% and 76%, positive predictive value of 67%, negative predictive value of 94% and reliability level of filter paper were 0.5 is moderate category. This indicates that the filter paper can be used as an alternative method of blood transport medium for rabies ELISA test
Efektivitas Larutan Desinfektan Dalam Menginaktivasi Virus Avian Influenza pada Bulu Unggas
No full textAvian Influenza (AI) virus is pathogenic agent that can spread from one area to another area through the transportation of infected animals or their products such as feathers. This research was aimed to inactivated AI virus with 775 ppm sodium hypochlorite and 0.1% chloroxylenol to examine the difference of treatment by time (day) for inactivation AI virus on the feathers. AI virus isolate A/chicken/sidrap/ 07160336‐2/2016 used in this research was obtained from Balai Besar Veteriner Maros. The treatment of disinfectants were performed on the first day (the day of disinfectan solutions were prepared), the third day and the seventh day by soaked the feathers in disinfectant solution for 10 minutes. The effectiveness of disinfectans were evaluated by inactivation index. The result show that the average of inactivation index of 775 ppm of sodium hypochlorit was 4.17 for the first day, 5.17 for the third day, and 4.20 for the seventh day, while the average of inactivation index of 0.1% chloroxylenol was 5.50 for the first day, 6.43 for the third day, and 5.77 for the seventh day. Our result indicated that the sodium hypochlorit and chloroxylenol were effective for inactivation of AI virus. The 0.1% of chloroxylenol was more effective for inactivation AI virus than 775 ppm of sodium hypochlorit, whilst the most effective duration for the treatment is the three days