Jurnal Sain Veteriner
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Analisis Hubungan Antara Pengetahuan dan Sikap Terhadap Praktik Penggunaan Antibiotika oleh Peternak Babi di Kota Kupang Provinsi Nusa Tenggara Timur
No full textThe prevalence of antibiotic resistance is an increasing problem due to the use of false and excessive antibiotics. The use of antibiotics in pig farms is increasing and needs to be aware of side effects, it is also influenced by the knowledge, attitudes and practices of farmers. This study is a cross-sectional study using a structured questionnaire interview to analyze the relationship between knowledge and attitudes towards the practice of using antibiotics by pig farmers in Kupang city. A total of 96 respondents pig farmers spread over 6 districts, District of Maulafa (9 villages) District of Alak (12 villages) District of Oebobo (7 villages), District of Kelapa Lima (5 villages), District of Kota Raja (2 villages) and the district of Kota Lama (1 village). The variables observed were individual characteristics, knowledge, attitudes and practices of the use of antibiotics by pig farmers in relation to the occurrence of antibiotic resistance. The relationship between the variables was tested using the Pearson correlation, while the relationship and magnitude of the direct and indirect impact on the observed variables was determined using path analysis. The results showed knowledge has a very significant impact (p <0.01) and indirectly contributes to the level of antibiotic usage practices preventing the occurrence of antibiotic resistance. Attitudes have a significant effect (p <0.05) and directly affect the practice of antibiotics to prevent the use of antibiotic resistance in Kupang. Conclusion of this study shows that knowledge has a major impact on the practice of using antibiotics by pig farmers, so by increasing farmers' knowledge of the facts incidence of antibiotic resistance, the attitude of farmers will increasingly have a positive significant effect on the level of practice use of antibiotics Prevent antibiotic resistance at a pig farm in the city of Kupang
Kajian Kliniko-patologik dan Antimikroba Ekstrak Biji Alpukat (Persea Americana Mill)
Get PDFThe phytochemical components of avocado seeds (Persea americana Mill) consist of alkaloids, flavonoids, tannins and saponins, so that they have the potential as antibacterials. The aim of this study was to determine the ability of Persea americana Mill extract to inhibit the growth of Staphylococcus aureus through inhibition zone observation and the ability to accelerate wound healing infected by S. aureus through observation of wound closure and cytopathology. Persea americana Mill were extracted using maceration method with 70% ethanol. The activities of this extract were tested by diffusion disc agar method. Biomedical tests were carried out on anaestetic Wistar rats whose were S. aureus infected wounds and treated with Persea Americana Mill extract. Based on the results of the antimicrobial test, avocado seed extract has a inhibitory effect on the growth of S. aureus at a concentration of 6.25% and best at a concentration of 100% compared to the control. Tests in experimental rats showed the effect of wound healing on the 2rd day after giving the extract Persea americana Mill ointment, faster than the control, which was need 4 days of wound closure. Cytopathology results showed that leukocyte activity was more prominent in rats treated with avocado seed extract compared to control rats. Avocado seeds as byproducts have the potential to be new herbal medicines that can be used as antimicrobials of S. aureus which have been confirmed to be resistant to various antibiotics
Seroprevalensi Penyakit Egg Drop Syndrome pada Itik di Desa Tumbak Bayuh, Kecamatan Mengwi, Badung, Bali
Get PDFEgg Drop Syndrome can cause detrimental impacts on breeders due to reducing production and quality of theaffected eggs.This study aim was to determine the seroprevalece ofantibody against Egg Drop Syndrome (EDS) virus in ducks. Antibody titers examination was done from 75blood samplesof ducks thathave not been vaccinatedagainstEDS virus. The duck samples were collected from Tumbak Bayuh Village, Mengwi, Badung. Serological examination was held at the Virology Laboratory,Faculty of Veterinary Medicine, Udayana University by usingHaemagglutination Inhibition (HI) test. HI test results showed that 24 samples were positive contained antibody of the EDS,while 51 samples were negative. Range of EDS antibody titer observed was from24 to 27 HI units. This results indicates that the ducks have protective antibody titer against EDS virus. The positive serum samples were also tested using HI test against Newcastle Disease (ND) virus with negative result. It can be concluded that the ducks in Tumbak Bayuh Village, Mengwi, Badung have 32% of antibody titer against EDS virus whichmight result from being exposed by EDS virus naturally
Pelacakan eskpresi protein pada testis mencit (Mus musculus) setelah paparan esktrak etanol daun mimba (Azadirachta indica)
Get PDFAbstract Azadirachta indica (Neem) has been shown to affect the fertility of mice by interfering with the synthesis of testosterone in mice. The aim of this study was to detect the testes protein expression of mice after exposure to the ethanolic Neem leaf extract. The laboratory animals of this study were 20 male Swiss Webster mice with three months in age and body weight ranging from 27.5 grams. The mice were divided into two treatment groups, namely K (control group, exposed with distilled water) and P (treatment group, exposed to etahnolic Neem leaf extract with 14 mg/animal /day). The treated were given for 21 days and the testicular protein was carried out on the 22nd day. The variables observed were testes weight, concentration and expression of proteins isolated from the testes. The protein concentration is determined by a spectrophotometer at a wavelength of 450nm. The protein expression was observed and determined based on the results of protein electrophoresis (SDS-PAGE). The results showed that protein expression in the treatment group has a lower concentration compared to the control group. Those results was confirmed by thinner bands in SDS-PAGE result. Those proteins thought to be a fertility determinant in mammals. Keywords : anti-fertility; Neem; protein expression Abstrak Azadirachta indica (Mimba) telah terbukti mempengaruhi fertilitas mencit dengan cara mengganggu sintesis hormon testoteron pada mencit. Penelitian ini bertujuan untuk melacak ekspresi protein pada testis mencit setelah paparan ekstrak etanol daun Mimba. Hewan uji penelitian ini adalah 20 ekor mencit Swiss Webster jantan dengan umur tiga bulan dan bobot badan berkisar 27.5 gram. Hewan uji dibagi menjadi 2 kelompok perlakuan, yaitu K (kelompok kontrol, dipapar akuades) dan P (kelompok perlakuan, dipapar dengan ekstrak etanol daun Mimba dengan dosis 14 mg/ekor/hari). Pemberian bahan uji dilakukan secara oral selama 21 hari. Variabel yang diamati adalah bobot testes, konsentrasi serta eskpresi protein yang diisolasi dari testis. Isolasi protein testis dilakukan pada hari ke-22. Konsentrasi protein ditentukan dengan spectrofotometer pada panjang gelombang 450nm. Ekspresi protein diamati dan ditentukan berdasar hasil elektroforesa protein. Hasil penelitian menunjukkan bahwa ekspresi protein pada kelompok perlakuan menunjukkan konsentrasi yang lebih rendah dengan pita yang lebih tipis jika dibandingkan dengan kelompok kontrol. Kesimpulan penelitian ini adalah paparan ekstrak etanol daun Nimba menyebabkan gangguan ekspresi protein yang diduga berperan dalam menentukan fertilitas mamalia. Kata kunci : Mimba; anti-fertilitas; eskpresi protein;
Uji Ekstrak n-Hexana Rumput Kebar (Biophytum petersianum Klotzsch) pada Tikus Wistar Hiperkolesterolemia
Get PDFThis study was aimed to determine the secure level of the n-hexane extract of kebar grass on hypercholesterolemia rats based the acute oral toxicity test. Based the literature, no report on the toxic dosage before, initial dose was started at 300 mg/kg body weight (BW) and to be increased 2000 to 5000 mg/kg BW. Toxicity data were set for 24 hours and continued until 14 days after treatment for several parameters, i.e : toxicity symptoms, death of animals, changes in body weight and the manifestation of toxicity effects. At the end of the test, the rats were sacrificed and organs were taken for abnormalities (macroscopy). The results of this study showed that the administration of the n-hexane extract of grass kebar at all doses did not show the toxicity symptoms, mortality and body weight change. The absolute and relative weights and gross pathology observation of the internal organ were normal. Conclusions: the n-hexane extract of grass kebar is safe and the LD was determined on category 5, 50 unclassified or minimum practically non toxi
MYCOTOXIN BINDERS EFFECT ON OVARIES OF PREGNANT MICE EXPOSED ZEARALENONE
No full textMycotoxin, a secondary metabolite produced by mushroom, has proven to cause various cytotoxic effects to animals. Zearalenone is one type of mycotoxin which is produced by Fusarium graminearum mushroom and has a cytotoxic effect to the reproduction system and cattle productivity by inducing apoptosis in the animal’s ovaries, uterus, and placenta. This research tested the potentials of mycotoxin binders in eliminating the effect of zearalenone which can be seen from the expression of caspase 3, the content of malondialdehyde (MDA), and the quantity of corpus luteum in the ovaries of pregnant mice (mus musculus). Twenty mice were grouped into five different treatments, namely K+ (not exposed to zearalenone and mycotoxin binders), K- (0.1 mg/mouse/day of zearalenone dosage), P1 (0.1 mg/mouse/day of zearalenone dosage and 0.5 mg/mouse/day of mycotoxin binder dosage), P2 (0.1 mg/mouse/day of zearalenone dosage and 1 mg/mouse/day of mycotoxin binders), and P3 (0.1 mg/mouse/day of zearalenone and 2 mg/mouse/day of mycotoxin binders), with 10-days treatment time. The results showed that the potentials of mycotoxin binders could be found on the gradual decline in the expression of caspase 3 in P1, P2 and P3 compared to that in K- (negative control). The results also showed a significant difference in the content of malondialdehyde (MDA) in K- compared to that in K+, P1, P2, and P3. Meanwhile, the quantity of corpus luteum in K- was also starkly different to that in K+, P1, P2, and P3. It can then be concluded that mycotoxin binders are very effective in eliminating the exposure of zearalenone
The Efficacy Study of Duramectin, Oxfendazole, Piperazine, and Pyrantel pamoate Against Gastrointestinal Worms In Horses In Yogyakarta Special Region*)
Get PDFThis research aimed at determining the efficacy of duramectin, oxfendazole, piperazine, and pyrantelpamoate against gastrointestinal worms in horses in Yogyakarta Special Region and Central Java. The object of research involved 40 horses diagnosed with gastrointestinal worm infection. Prior to the research, all of the horses were subjected to examination for clinical symptoms and parasitology laboratory checkup for signs of worm eggs in their feces, and they were pronounced positive for experiment animals with at least 150 eggs per gram of feces per horse. The research horses were weighed to determine the dose of worm medication to be used. The research horses are divided into four treatment groups, each group consists of ten horses. Group I was given duramectin with a dose of 0.2 mg/kg of body weight. Group II was given oxfendazole with a dose of 7.5 mg/kg of body weight, Group III was given 125 mg Piperazin treatment per kilogram of body weight and Group IV was given pyrantelpamoate with a dose of 20 mg/kg of body weight. After receiving themedication, the horses were observed to document the progress in terms of clinical symptoms and the amount and type of worm eggs in their feces every three days for four times in a row. The research data results were tabulated and were descriptive-comparatively analyzed.The research result showed varying efficacy level of worm medication for horses. Oxfendazole kills Strongylus and Parascaris worms. Duramectin kills Strongylus worms and reduce some of the Parascaris worms. Piperazineandpyrantelpamoate kills the Strongylus wormsand reduce some of the Parascaris.
Studi Reseptor Virus Infectious Bursal Disease (IBD) pada Organ Limfoid Ayam Pasca Vaksinasi dengan Metode Imunohistokimia
Get PDFInfectious bursal disease (IBD) affects economical impact for breeders due to it can cause damage lymphoid organ, especially bursa of Fabricius and causing failure the vaccination program. Infectious bursal disease virus receptors on lymphoid organs suspected contribute to subclinical and clinical IBD incidence in the chickens. The aim of this study was to determine and compare the distribution of IBDvirus receptors on lymphoid organ of the chicken which obtained different IBD vaccination program. The presence of virus receptors in bursa of Fabricius, spleen, and thymus were observed microscopically using immunohistochemical method and evaluated with Image J® software. Monoclonal anti LSCC-BK3 (Gifu University, Japan) antibodies as primary antibody was used in this study. The result showed that IBDvirus receptors found abudantly in the bursa of Fabricius, afterwards in the spleen and thymus. No significant differences of IBD virus receptor distribution within lymphoid organs between chicken which obtained once and twice IBD vaccination. Infectious bursal disease virus receptor distribution in bursa Fabricius chickens aged 23 days received twice IBD vaccination more than once IBD vaccination program
Pengaruh Pemberian Serum Albumin Manusia terhadap Kadar Albumin dalam Darah pada Anjing dengan status Hipoalbuminemia
Get PDFAlbumin consists of 50% of the total plasma protein and it is very important protein in the animal's body. Hypoalbuminemia can occurs due to various conditions, such as loss of protein nephropathy, loss of enteropathic proteins, chronic and acute diarrhea or heavy bleeding as well as malnutrition and malabsorption conditions. This study aims to determine the effect of human serum albumin 20% infusion to increase albumin blood level in hypoalbuminemia dogs. Animals used in this study were 5 dogs whose results showed the levels of albumin below normal limits or hypoalbuminemia. Dogs with hypoalbuminemia will be infused with human serum albumin (HSA) 20% through intravenous for ± 4 hours. Then, these dogs blood will be drawn days after infusion to screen for albumin levels. The results obtained in the form of examination data of albumin blood levels in the blood before and after infusion will be analyzed with paired T-test. The results showed an increase of albumin level significantly from an average of 1.98 ± 0.356 to 2.28 ± 0.257(P <0.05). This study shows the presence of human albumin serum through intravenous infusion was able to increase albumin levels in dogs with hypoalbuminemia
Klebsiella pneumoniae Isolated from Subclinical Mastitis Milk of Etawah Crossbreed Goat
No full textKlebsiella spp. is a common bacteria causing mastitis. Klebsiella pneumoniae and Klebsiella varicola is the most infected Klebsiella spp in the dairy farm. This study used 16 milk samples from 8 lactation goats in Sahabat Ternak farm, Sleman, Yogyakarta. Samples were tested by california mastitis test (CMT) reagent to determine the mastitis status, inoculated on blood agar (BA) then on Mac Conkey Agar (MCA) as a selective media for Gram negative bacteria. The colonies from BA and MCA were stained by Gram staining to determine Gram-positive or Gram-negative bacteria and its cell morphology. The colonies from MCA were identified by biochemical tests such as sugar fermentation tests (glucose, lactose, maltose, and saccharose), arginine, ornithine decarboxylase, indole, citrate, urea hydrolysis (Christensen’s method), lysin and malonate. The result of mastitis test showed 5 goats were positive result and 3 samples were negative. Bacterial staining showed 2 samples were Gram-negative, rods and the others were Gram-positive, coccus. The samples with rods shapes were continued by biochemical tests. The characterization result of biochemical test indicated that the rods shapes bacteria were Klebsiella pneumoniae. These bacteria identification conducted in Laboratorium Preklinis Program Studi Kesehatan Hewan, Sekolah Vokasi Universitas Gadjah Mada and the result confirmation using positive control of Klebsiella pneumoniae conducted in Balai Laboratorium Kesehatan Yogyakarta. These results showed that milk from Etawah crossbreed goat was infected by Klebsiella pneumoniae