Jurnal Sain Veteriner
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Pengaruh Ukuran Ovarium dan Folikel terhadap Penampilan Reproduksi Pada Sapi PO dan SimPO di Kecamatan Jatinom, Kabupaten Klaten
Get PDFThe increasing beef cattle population in Indonesia is not significantly with high demand of meat every year. The insignificant increasing in population is caused by fertility decreasing, infectious disease and the reduction amount of forage land. The factor which causes cattle fertility decreasing can’t be separated from the ovarian and follicular diameter which have an effect on high various reproductive appearance. Parameters that used for knowing reproductive appearance of cattle are Service per Conception (S/C) and Calving Interval (CI). The aims of this study was to describe ovary sizes and follicular diameter in cattle, to describe ovary and follicular diameter toward S/C and CI variation. This research uses female cattle, 8 Simmental-PO (SimPO) and 9 Peranakan Ongole breeds (PO), multiparous, are not pregnant, 4-8 years old, Body Condition Score (BCS) 2.5-3.5, peak phase estrus, has no reproductive problems, clear recordings and live in the area of farmer in Jatinom District, Klaten Regency, Central Java Province. Cattle are examined for ovarian size and follicle size using ultrasonography. S/C and CI data were obtained from records of artificial insemination cards (AI). The data obtained were analyzed by t test. The results of this study showed there were no differences in the diameter of ovaries in the size of SimPO and PO cattle (P> 0.05), there were no differences in the diameter of SimPO and PO cattle follicles (> 0.05), there were differences in ovarian and follicular diameter sizes with respect to S/C and CI in cattle with good and bad reproductive performance (P 0.05) but there was an influence on S/C and CI (P <0.05).
PENILAIAN ORGANISASI PERANGKAT DAERAH (OPD) TENTANG OTORITAS VETERINER DALAM KEBIJAKAN PENANGGULANGAN RABIES DI PROVINSI BANTEN
Get PDFAbstractLegislation No 41 Year 2014 about Amendment of Legislation No 18 Year 2009 about Livestock and Animal Health explains that Veterinary Authority is a government institution, formed in order to deal with decision making of anything related to animal health, by involving veterinarians and mobilizing all related professional lines, to identify problems, deciding policies, coordinating policy implementation, and handling the field operations.This research material uses the result from a group of respondents, which represents 36 Regional organizations (OPD), they are: 9 OPD Technical services, supervising animal health (keswan) and veterinary public health (kesmavet), 9 OPD Health services, 9 OPD Regional development planning services, and 9 OPD Regional staffing services (BKD), for regencies, and cities in Banten Province. Data collection is done using two methods, quantitatively by using data from all 36 OPD, and qualitatively by using observation, interviews, dan questionnaires.First 5 questions: 1). Are the organization bureaucratic? 2). Positions, roles, and authority of veterinarians? 3). Is the veterinary authority regarding rabies control at agencies, clinics, and animal health center already in line with the implementation? 4). Strategic policy and 5). Integrated, directed, and continuous program regarding public awareness of rabies, for rabies control in provincial or regency government. The answers are as follows, Technical services of animal health (keswan) and veterinary public health (kesmavet) : 0%, 33,33%, 77,78%, 100% and 77,78% answered yes; 100%, 66,67%, 22,22%, 0% and 22,22% answered no. Health services: 22,22%, 66,67%, 88,89%, 44,44% and 44,44% answered yes; 77,78%, 33,33%, 11,11%, 55,56% and 55,56% answered no. Regional development planning services (Bappeda), 33,33%, 88,89%, 66,67%, 11,11% and 11,11% answered yes; 66,67%, 11,11%, 33,33%, 88,89% and 88,89% answered no; Regional staffing services (BKD) 88,89%, 77,78%, 55,56%, 0% and 0% answered yes; 11,11%, 22,22%, 44,44%, 100% and 100% answered no. Next questions: 6). Cooperations between OPD, 7). Regional Policy 8). Veterinarian openings and 9). Enforcement and improvement of otovet institutions. The answers are as follows: Technical services of keswan kesmavet: 33,33%, 44,44%, 77,78% and 100% answers yes; 66,67%, 55,56%, 22,22% and 0% answers no. Health services: 55,56%, 22,22%, 0% and 77,78% answers yes; 44,44%, 77,78%, 100% and 22,22% answers no. Regional development planning services (Bappeda), 33,33%, 33,33%, 33,33 and 100% answers yes; 66,67%, 66,67%, 66,67% and 0% answers no; while Regional staffing services (BKD) 11,11%, 11,11%, 100% and 66,67% answers yes, 88,89%, 88,89%, 0%, !00% and 33,33% answers no.In conclusion, after scoring the questionnaire, the results for OPDs supervising animal health (keswan) and veterinary public health (kesmavet) otovet are as follows: Technical services with 9 OPDs, 4 OPDs scores well and 5 OPDs scores poor. Health services with 9 OPDs, 4 OPDs scores well and 5 OPDs scores poor. Regional development planning services (Bappeda) with 9 OPDs, 4 OPDs scores well and 5 OPDs scores poor. Regional staffing services (BKD) with 9 OPDs, 1 OPD scores well and 8 OPDs scores poor.Keywords: Veterinary authority; Regional organizations (OPD), control rabies, rabie
Karakteristik Avibacterium paragallinarum Isolat Lapang : Inokulasi pada Telur Ayam Berembrio umur 7 hari
No full textInfectious coryza (IC) adalah penyakit bakterial yang menyerang saluran pernapasan ayam, yang dapat bersifat akut sampai kronis yang disebabkan Avibacterium paragallinarum. IC merusak saluran pernapasan bagian atas, terutama rongga hidung. Pengamatan perubahan makroskopik pada embrio yang mengalami kematian setelah inokulasi pada telur ayam berembrio (TAB) specific pathogenic free (SPF) sebagai salah satu karakteristik isolat A. paragallinarum belum banyak dilakukan. Penelitian ini bertujuan untuk mengetahui karakteristik isolat A. paragallinarum melalui inokulasi pada telur ayam berembrio TAB SPF umur 7 hari. TAB SPF umur 7 hari berjumlah 50 butir dibagi menjadi 5 kelompok masing-masing terdiri dari 10 butir yaitu kelompok kontrol negatif, A. paragallinarum serotipe A/221; serotipe B/Spross; serotipe B/2448; dan serotipe C/2447. Bakteri terlebih dahulu dikultur pada media cair dan diinkubasi selama 24 jam pada suhu 37oC. Suspensi bakteri dengan volume 0,2 ml (6x108 cfu/ml) diinjeksikan pada TAB SPF, diinkubasi pada suhu 37oC dan diamati adanya kematian sebanyak 2 kali sehari. Embrio yang mati dilakukan skoring perubahan makroskopik. Embrio yang berasal dari TAB SPF pada kelompok yang diinokulasi A. paragallinarum serotipe A/ 221, B/Spross, B/2448, and C/2447 mengalami hemoragi dan kekerdilan. Keseluruhan isolat A. paragallinarum bersifat patogen pada telur ayam berembrio
Electrical stunning sebelum penyembelihan dapat menurunkan Serum Superoxide Dismutase Babi Landrace Crossbred
Get PDFPenelitian bertujuan untuk mengevaluasi enzim Superoxide Dismutase (SOD) pada serum babi Landrace Crossbred yang mendapat electrical stunning dan tanpa electrical stunning sebelum penyembelihan. Sebanyak 18 ekor babi Landrace Crossbred jantan dewasa kebiri dan bobot 115±20 kg dibagi menjadi dua perlakuan, yakni electrical stunning dan tanpa electrical stunning dengan pengujian serum pada pre dan post perlakuan. Sebanyak 3 ml sampel darah diambil dan diuji kadar SOD dengan StressXpress® kit. Pembacaan kadar SOD diuji normalitas dengan Shapiro-Wilk Test, diilanjutkan uji Univariate Linear Model untuk membandingkan kadar SOD antara electrical stunning dan tanpa electrical stunning pada fase pre dan post perlakuan. Hasil Shapiro-Wilk Test dengan diagram Q-Q plot menunjukkan pola distribusi normal pada semua perlakuan. Kelompok electrical stunning menunjukkan penurunan signifikan SOD. Kadar SOD yang rendah tampak signifikan juga pada pre perlakuan dibandingkan dengan post perlakuan. Dapat disimpulkan terdapat penurunan signifikan SOD pada serum babi Landrace Crossbred yang mendapat electrical stunning sebelum penyembelihan
Faktor Risiko Feline Panleukopenia pada Kucing di Daerah Istimewa Yogyakarta
Get PDFAbstractFeline panleukopenia virus (FPV) infection in cats caused a disease called feline panleukopenia with high morbidity and mortality worldwide. There is no causative therapy for this disease yet, therefore knowing risk factors is important measure to prevent the disease. This study was designed to investigate the risk factors of feline panleukopenia in Special Region of Yogyakarta therefore disease prevention can be done as early as possible. A retrospective case-control study that used 35 cats in the case group and 70 cats in the control group. Case group consisted all feline panleukopenia patients that were tested positive with Anigen FPV Ag Test Kit (Bionote, South Korea) from two local clinics. Control group consisted cats that were own by the neighbour of cats from the case group with age and sex as matching variables. Owner characteristics including formal education, occupation, duration of having cat(s), monthly maintenance cost, and interaction time per day also feline characteristics including breed, neuter status, origin, husbandry, and vaccination status were assessed. Association analysis was done using EpiInfo (chi-square and odds ratio) and manually calculated (estimated attributable fraction and estimated population attributable fraction). Associated risk factors of feline panleukopenia were duration of having cat(s) less than a year, domestic cat breed, cat origins such as pet store or market and stray cat, the presence of new cat(s), outdoor cat lifestyle, waste disposal outdoor, and not vaccinated cat. Vaccination status and the presence of new cat(s) had the highest strength compared to other risk factors. It can be concluded that vaccination and quarantine of new animals are important measures to prevent feline panleukopenia.Keywords: case-control study, cats, feline panleukopeni
Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus Isolat Preputium Sapi Aceh (Antibiotic Activities To Staphylococcus aureus Biofilms Of Aceh Cattle Preputium Isolate)
Get PDFAbstractBiofilm is one of parameters for determining pathogenic pathways of a bacterium. Bacterial virulence activities can be attenuated by antibiotics with inhibiting biofilm. The aim of this study was to examine the characteristics and activities of antibiotics against the biofilm of Stapylococcus aureus isolate which taken from Aceh cattle prepuce. The study was exploratory and experimental, using a Completely Randomized Design (RAL) factorial pattern using three types of antibiotics, three concentrations and replications. Antibiotics were tetracycline, oxytetracycline and phosphomycin with concentrations of 10%, 20% and 30% (mg/mL), respectively. Therefore, the aim of this study was to examine the characteristics and activities of antibiotics to the biofilm of Stapylococcus aureus from Aceh cattle prepuce isolate. U-bottom polystyrene 96 wells microplate and Nutrient Broth (NB) medium were used to biofilm. Bacterial while biofilm was stained on crystals violet 1%. Optical Density (OD) of biofilm were λ 595 nm. Data were analyzed by using Analysis Of Variance (ANOVA). The results showed that the activity of Staphylococcus aureus was moderately positive, i.e 2,667cell/μL. It showed that antibiotics and concetrations have significant effect (P<0.05) to inhibit biofilm staphylococcus aureus. In additioon, tetracycline 30% (mg / mL) has the best ability to inhibit biofilms from 52,18% to 1,262 cell/μL. Based on that fact, it can be concluded that the virulence of Staphylococcus aureus was moderate positive to be false positive. Keywords: biofilm, antibiotics, Staphylococcus aureus, cows aceh. Abstrak Biofilm merupakan salah satu parameter untuk menentukan jalur patogenisitas suatu bakteri. Antibiotik dapat melemahkan aktivitas virulensi bakteri dengan menghambat pembentukan biofilm. Penelitian ini bertujuan untuk melihat karaksteristik dan aktivitas antibiotik terhadap biofilm Stapylococcus aureus isolat preputium sapi Aceh. Penelitian bersifat eksplorasi dan eksperimental, menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan menggunakan tiga jenis antibiotik, tiga konsentrasi dan tiga kali ulangan. Antibiotik yang digunakan adalah tetrasiklin, oksitetrasiklin dan fosfomisin dengan konsentrasi masing-masing 10%, 20% dan 30% (mg/mL). Pengujian biofilm dilakukan pada media Nutrient Broth (NB), menggunakan mikroplat U-bottom polystyrene 96 wells. Pewarnaan biofilm menggunakan kristal violet 1%. Optical Density (OD) biofilm dibaca pada λ 595 nm. Data hasil penelitian dianalisis dengan menggunakan Analysis Of Variance (ANOVA). Hasil penelitian menunjukkan aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm tergolong positif moderat, yaitu 2,667cell/µL. Uji Biofilm menunjukkan jenis dan konsentrasi antibiotik berpengaruh secara nyata (P<0,05) terhadap daya hambat biofilm Staphylococcus aureus isolat preputium sapi Aceh. Tetrasiklin 30% (mg/mL) mempunyai kemampuan paling baik menghambat pembentukan biofilm Staphylococcus aureus isolat preputium sapi Aceh sebesar 52,18% menjadi 1,262 cell/µL, sehingga aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik biofilm dari positif moderat menjadi positif palsu. Kata Kunci: biofilm, antibiotik, Staphylococcus aureus, sapi aceh
Gonadotropine Releasing Hormone (GnRH) Receptor Profile from Cow’s Hypothalamus
No full textGonadotropine Releasing Hormone (GnRH) is one of the recommended hormones to overcome ovulation problems and it can increase pregnancy rate so that it is used in government programs to increase cattle population in Indonesia, although the results are not yet optimal. The purpose of this study is to compare the composition of bovine GnRH receptor nucleotides with the GenBank reference so that the level of genetic diversity of bovine receptors in Indonesia is known. PCR product sequencing using Promoter F and Exon 1 R primers were further aligned with the reference sequences of Bos Taurus GnRHR mRNA GenBank using the MEGA X program. The results of the analysis found the presence of Single Nucleotide Polymorphism (SNP) in the coding region of 1st cow position 38(A> T), 261(C > T), 342(C >T), 411(C >T) and 495(C > T) and 2nd cow positions 261(C >T). Changes in amino acids were also detected in 1st cow position 13 (H> L). The presence of SNP was found to indicate genomic variation between individuals at cattle receptors in Indonesia
Faktor Risiko Cemaran Escherichia coli pada Daging Kambing dan Domba Kurban di Provinsi DKI Jakarta
Get PDFEscherichia coli merupakan salah satu bahaya biologis yang mempengaruhi keamanan daging kambing dan domba Kurban. Penelitian ini ditujukan untuk mengukur tingkat kontaminasi dan menentukan faktor risiko yang mempengaruhi tingkat cemaran E. coli pada daging kambing dan domba kurban di Provinsi DKI Jakarta. Penelitian ini menggunakan data sekunder dari hasil pemeriksaan kesehatan hewan dan daging Kurban. Tingkat kontaminasi E.coli diperoleh dari hasil pemeriksaan laboratorium dengan metode MPN, sedangkan data faktor risiko diperoleh dari penilaian kelayakan tempat kurban menggunakan checklist yang dikembangkan oleh tim Fakultas Kedokteran Hewan IPB University. Data dianalisis menggunakan uji chi-square untuk menentukan asosiasi dan rasio odds (OR) untuk mengukur kekuatan asosiasi. Hasil penelitian ini menunjukkan bahwa 66,7% tempat penyelenggaraan kurban telah menghasilkan daging kambing dan domba yang mengandung E. coli dengan level melampaui batas Standar Nasional Indonesia/SNI (1 × 101 MPN / g). Faktor risiko selalu membersihkan kotoran hewan memiliki hubungan yang signifikan dengan tingkat kontaminasi E. coli. Faktor risiko lain termasuk keberadaan sertifikat kesehatan hewan, kepadatan hewan, dan bagaimana hewan ditangani setelah disembelih (hewan tidak ditumpuk) juga cenderung memiliki hubungan dengan tingkat kontaminasi E. Coli meskipun tidak signifikan secara statistik. Penyelenggara Kurban harus meningkatkan praktik higiene dan sanitasi dalam mengolah daging untuk mengendalikan tingkat kontaminasi E.coli. Pemerintah Provinsi DKI Jakarta perlu meningkatkan upaya untuk memperbaiki kesadaran masyarakat melalui komunikasi, edukasi dan informasi terkait praktik-praktik penanganan daging yang baik untuk Kurban
Methionine Hydroxy Analog Supplementation to Increase Feed Utilization for Indigenous Sheep
No full textIn the tropical area, productivity of ruminant has not optimized caused by the low quality of nutrition that leads to low-efficiency metabolism at the level of ruminal fermentation, post rumen digestibility, and intermediary metabolism. The study aimed to analyze effect of methionine hydroxy analog (MHA) on ruminal fermentation profiles of indigenous sheep specifically in the increase of ruminant productivity. In vitro utility test was conducted using rumen fluid of the indigenous sheep and sample of rational ration having a proportion of grass and concentrate 30%:70%, dry matter basis. The treatments implemented were three levels of MHA supplementation; T0: 0 g/day, T1: 3 g/day, and T2: 6 g/day. Variables measured were dry matter digestibility (DMD), organic matter digestibility (OMD), production of VFA, NH3, as well as total protein, and molar proportion of partial VFA of rumen fluid. Data were analyzed using analysis of variance (ANOVA) in a completely randomized design (CRD). The 0.2% MHA supplementation increased OMD with the highest production of total protein was from 28.57 mg/g (T0) to 40.49 mg/g (T2) (P<0.05). Meanwhile, the lowest ratio of acetate : propionate was from 2.74 (T0) to 2.33 (T2) (P<0.05). Supplementation of MHA up to 6 g/day concentrate increased the performance of fermentation and/or feed utility.
Profil Darah Rusa Totol (Axis axis) Betina Sehat di Pusat Inovasi Agroteknologi (PIAT), Universitas Gadjah Mada
Get PDFCenter for Agrotechnology Innovation, Gadjah Mada University (PIAT), one of the place for development and breeding ground of spotted deer in Yogyakarta, Indonesia. The study of blood profiles was intended to allow veterinarian to understand the normal profile of the spotted deer. Fifteen healthy spotted deers belonging to PIAT were used as experimental animals. The deers were drawn its blood through the jugular vein without the use of anaesthesia. Blood was then accommodated in an EDTA tube, centrifuged at 2500 RPM and analyzed using a Mindray BC-2800 haematology analyzer machine. Based on the results of the study of the blood it were known that Haemoglobine (Hb) was: 11.5 ± 1.703 g/dl, Red blood cells (RBC) was: 9.3 ± 3.580 106/µl, Packed cell volume (PCV) was: 30.8 ± 6.035 %, Mean corpuscular Volume (MCV) was: 36.8 ± 11.102 fl, Mean corpuscular haemoglobine (MCH) was: 15.0 ± 7.313 pg, Mean corpuscular haemoglobine concentration (MCHC) was: 40.0 ± 14.657 g/dl, White blood cells (WBC) was: 6.4 ± 3.096 103/µl , Neutrophils was: 43.4 ± 21.646, % Basophils was: 0.2 ± 0.168 %, eosinophils was: 0.4 ± 0.447 %, lymphocytes was: 53.4 ± 21.546 % and monocytes was: 2.6 ± 2.394 %. From all of the datas it indicated that blood profiles of PIAT’s spotted deers are different from similar spotted deer blood profiles in India