Jurnal Sain Veteriner
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    ANALISIS KUALITAS DAGING AYAM BROILER ASAL PASAR SWALAYAN DAN PASAR TRADISIONAL DI KOTA MEDAN SUMATERA UTARA

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    The mean protein content in broiler chickens from high to low were respectively protein in breast meat from supermarkets: 16.83 ± 0.42 %; breasts from the traditional market: 15.63 ± 1.09 %, thighs from the supermarket: 14.5 ± 0.57 % and thighs from the traditional market: 13.6 ± 0.38 gr / 100 %. Based on all of the datas collection,  the result of statistical analysis using a 2x2 factorial pattern showed that there were no significant differences of water content (P>0.05) whreas: the ash, carbohydrates, fats and proteins showed significant (P£0.05).Thighs from traditional markets 4.5 ± 0.60 % and chest from traditional markets from 5.3 ± 0.69 %. The Average of fat content of broiler meat in the thigh, from supermarket: 2.56 ± 0.63 %; chest origin supermarket 1.2 ± 0.5 %; thigh meat from traditional markets: 3.15 ± 0.21 % and breast meat from traditional markets: 1.8 ± 0.227 %. Broiller is one of the biggest contributor to animals protein from livestock and is a superior commodity in Indonesia. At present, the broiler chicken industry has developed rapidly and become the largest contributor to animal protein as well as a major source of consumer menus that are very easy to obtain, both in modern and traditional markets. After the achievement of the population of broiller chickens, government policy began to emphasize on improving the quality of meat by changing meat characteristics such as appearance, texture, moisture content, firmness, softness, odor, taste and the nutritional content is no exception. Thirty-two samples of broiller chicken meat consisting of 16 meat samples purchased from 4 supermarkets and 16 samples purchased from 4 traditional markets were used as research objects. A hundred grams of fresh meat per sample were purchased and immediately packed in aluminum foil, packaged in a cooler box then sent to the Food and Nutrition Laboratory, Inter-University Center, Gadjah Mada University for proximate analysis. The results of the examination of water concentration showed that the average of water content of thigh meat from supermarkets was 76.26 ± 0.86 %; supermarket breast meat 74.135 ± 0.92 %; thigh meat from the traditional market 75 ± 0.56 % and breast meat from the traditional market: 75.64 ± 1.044 %. Analysis of the average levels of the ash concentration respectively: thighs from the supermarket 0.96 ± 0.027 %, chest from the supermarket 1.095 ± 0.05 %, thighs from the traditional market 1.034 ± 0.106 % and breasts from the traditional market 1.155 ± 0.11%. The average of carbohydrate levels were consecutive: Thigh meat from the supermarket: 5.6 ± 1.33 %; chest of origin of the supermarket: 6.7 ± 1.078 %

    Faktor Risiko Potensial terhadap Canine Leptospirosis di Ragunan Animal Hospital Jakarta, Indonesia

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    Leptospirosis is a zoonotic disease caused by bacteria Leptospira sp. which causes infection in animals and humans. Dogs infected with leptospirosis showed symptoms such as anorexia, fever, vomiting, weakness, diarrhea and often experience yellowing of the eye area and mucosa around the mouth (icteric) with fatal systemic complications and multi-organ dysfunction, especially in the kidneys and liver. Leptospirosis is an endemic disease in Jakarta. This study aims to identify risk factors that can contribute to canine mortality based on early clinical symptoms that are found when the dog is in an animal health service facility such as a veterinary clinic, veterinary hospital or independent practice veterinarian. Method were used in this study is clinical manifestations and laboratory examinations and medical records of dogs with suspected leptospirosis. Criteria inclusion were based on aspects of the clinical symptoms of dogs in and around Jakarta. Analysis data used the chi-square with confidence of interval (CI) 95%. Dogs used during the study had ages for puppies (less than 1 year) totaling 13 or 32.50%, for adult dogs over 1 year amounted to 27 or 67.50%, 80% male dogs and 20% female. with 80% maintenance system not housed by the owner. Risk factors for clinical symptoms such as myalgia, symptomatic vomiting of the pulmonary area or shortness of breath and abdominal pain, conjunctival suffusion, anorexia and diarrhea contributed to the high mortality rate leptospirosis during study in dogs 2020

    Efektivitas Low Density Lipoprotein (LDL) dari Kuning Telur Ayam terhadap Kualitas Semen Cair Domba

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    Low-Density Lipoprotein (LDL) extracted from egg yolk (LDL) has recently known can eliminate the adverse effect associated in the use of fresh egg yolk. The role of LDL in liquid preservation at 4°C of ram sperm has not been explored. This research evaluates the effects of substituting egg yolk with LDL in ram sperm preservation at 4 °C on 5 days. The objective of this research was to assess the effects of substituting egg yolk with LDL for use as an extender in sperm preservation at 4°C, as well as on spermatozoa motility, viability, morphology, plasma membrane, and acrosome integrity, for 5 days. The semen was subsequently divided into five and diluted with Tris–fresh egg yolk (K), Tris–LDL5% (LDL5), Tris–LDL10% (LDL10), Tris–LDL15% (LDL15), and Tris–LDL20% (LDL20). The result showed a significant difference between LDL to fresh egg yolk for ram sperm quality (P<0.05). The effectiveness of LDL on sperm quality decreased following by its concentration. Even though up to 20% concentration of LDL, it can not preserve the quality of diluted semen for motility, viability, and plasm membrane integrity.

    Evaluasi Pemberian Obat Diminazene Aceturate Secara In Vivo Pada Mencit (Mus musculus) yang Diinfeksi Isolat Trypanosoma evansi

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    Abstract Surra's disease is caused by Trypanosoma evansi parasite has been established as one of the strategic infectious animal diseases. Drug resistance in this case is one of the major challenges in handle and control them. The aim of this study is to evaluate the provision drug resistance diminazene aceturate (Tryponil®) on Trypanosoma evansi isolate from Pemalang and Brebes Central Java province with in vivo test in mice. Total 50 mice, BALB / c strain, male, 2 months, body weight ± 30 gram are obtained from LPPT-UGM, adapted for one week. Mice were divided into 10 groups consist of 5 each. Each mouse was infected with Trypanosoma evansi by intraperitonial route. Treatment was given when mice had reached the level of parasitemia 108 – 109 trypanosoma / mL of blood this was predicted 24 hours post-infection (Eisler et al., 2001). The administration of the drug tryapanosidal was done intraperitonial with doses 1mg/kg, 3mg / kg, 5 mg / kg and 7mg / kg. Observation of parasitemia did every 2 times in one week till 60 days of observation. Parasitemia observation was performed using 3 techniques. The first method was native examination used a microscope, if the negative results would be followed by MHCT (Microhaematocrit centrifugation Technique) and BCT (Buffy Coat Technique) according to OIE (2012). Data obtained from the treatment group were the level of parasitemia, the number of deaths and the number of live mice from each test dose. The results are analysed by standard logit or probit. The results of this study showed the effects of the drug Dimianzene aceturate on both isolates varied. On Brebes Isolate was effective at doses of 7 mg / kg BW (100%) and 5mg / kg BW (80%), whereas in the effective dose Pemalang isolate at 3 mg dose / kg BW (80%), 5 and 7 mg / kg BW (100%). While at the lowest dose of 1 mg / kg obtained a level of effectiveness of 0% in both isolates. It could be concluded that both isolates have different pathogens and indicate resistance subpopulation to diminazene aceturate.Keywords : diminazene aceturate, in vivo, resistance, Trypanosoma evansi.

    Pemilihan Antibiotika pada Anjing Diare yang Terinfeksi Escherichia coli

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    Diare adalah penyakit yang sering ditemukan pada anjing dan Escherichia coli merupakan salah satu yang dianggap sebagai penyebabnya. Tujuan dari penelitian ini adalah menentukan diagnosis diare pada anjing yang disebabkan E. coli dan menentukan pilihan antibiotika sebagai terapi utama. Sebanyak 3 pasien anjing diare digunakan di dalam penelitian ini. Semua anjing diperiksa secara fisik dan E. coli diidentifikasi dari sampel fesesnya. Sampel feses dipupuk pada agar Harlequin™ E. coli/Coliform Medium dan diinkubasi pada 37 °C selama 24 jam. Koloni yang tumbuh diamati, dicat Gram dan diperiksa di bawah mikroskop. Bakteri selanjutnya diuji sensitivitasnya terhadap amoxicillin, chloramphenicol, doxycycline dan enrofloxacin menggunakan metode disk difusi. Zona pertumbuhan bakteri diukur dan dibandingkan dengan standar. Hasil penelitian menunjukkan bahwa E. coli ditemukan pada semua (3) sampel feses anjing diare. Semua bakteri tersebut sensitif terhadap chloramphenicol dan 1 isolat sensitif terhadap amoxicillin, tetapi isolat lainnya berifat intermedier sampai resisten terhadap amoxicillin, doxycylin dan enrofloksasin. Kesimpulan penelitian ini adalah E. coli dapat berperan sebagai penyebab diare  pada anjing dan chloramphenicol adalah antibiotika pilihan utama untuk terapi

    Respon Imun Seluler Ayam Petelur Pascavaksinasi Avian Influenza Subtipe H5N1 Isolat dari Bali

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    Avian Influenza subtype H5N1 (AI-H5N1) is a malignant virus that is very detrimental to laying chickens because it is highly contagious and mutates easily. Prevention of AI-H5N1 disease in laying chickens is carried out by vaccination, therefore to maintain the quality of the vaccine, continuous research is needed. This study aims to determine the potential of AI-H5N1 vaccine isolates from Bali as measured based on cellular immune response based on total and differential leukocyte cells. Formation of antibodies is influenced by the nonspecific and specific immune system involving leukocytes, especially lymphocytes. Total of 40 layers of Novogen Brown strain were used for the research sample, kept since the age of one day on a commercial farm in Perean Village, Tabanan Regency, Bali. Laying chickens are vaccinated at 5 weeks of age by intramuscular injection. Total of 20 chikens were taken randomly and used for the sample. Blood draws were performed once pre-vaccination and five times each week after vaccination with anticoagulants. Total leukocytes were examined by an auto hematology analyzer, while differential leucocytes with thin blood smear stained with Giemsa. Total and differential leukocyte data were analyzed by means of the variance test followed by the Duncan test. Results showed that AI-H5N1 vaccination from Bali isolates could increase total and differential leucocytes of laying chickens and had significant effect on the mean total leukocytes, the absolute values of heterophyll cells, eosinophils, lymphocytes, and monocytes, but had no significant effect on post-vaccination basophil cells

    Pengaruh Pemberian GnRH pada Sapi Potong yang Mengalami Kawin Berulang (Repeat Breeding)

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    Repeat breeding is a condition in which cows have normal estrous cycles and have been mated 3 times or more but are not pregnant. The incidence of repeat breeding in beef cattle can be detrimental to breeders in terms of time, that the calving period becomes longer so that the cows cannot give birth to calves once a year. Various ways to deal with repeat breeding have been done, but the results are not optimal. This study was conducted to determine the effect of Gonadotrophin Releasing Hormone (GnRH) in cows with repeat breeding. A total of fifteen beef cattle with repeat breeding were used in this study. The cows were divided into 3 groups of 5 each. Cows that were estrus in the first group (as a control group) were only performed artificial insemination (AI) without GnRH injection. In the second and third groups, after AI, they were injected with GnRH at AI time and 3-4 days after AI with a dose of 5 ml (Fertagyl®, 500 µg gonadorelin) intramuscularly. A pregnancy examination was carried out three months later and the results were recorded. The data obtained were analyzed descriptively and quantitatively using Chi-Square statistical analysis. The results of the analysis showed that there were differences in the pregnancy rate in the first group (control) with the second cow group and the third cow group, which were 20%, 80%, and 60% respectively. Based on statistical analysis, there was a significant difference in pregnancy rate (P <0.05) between the three groups. It was concluded that GnRH administration could increase the pregnancy rate in beef cattle that had repeat breeding

    Survei Prevalensi Ektoparasit pada Populasi Anjing Pemburu (Canis Lupus Familiaris) di Kecamatan Jagong Jeget Kabupaten Aceh Tengah

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    Anjing merupakan salah satu hewan domestik yang banyak digemari manusia sebagai hewan kesayangan karena pemeliharaannya yang relatif mudah. Keberadaan anjing sangat populer dikalangan masyarakat tertentu, karena bagi mereka, anjing dipelihara sebagai teman, penjaga, pemburu, pengintai atau pelacak narkoba. Disisi lain, untuk menjaga kondisinya tetap stabil sesuai kebutuhan pemeliharaannya, maka kesehatannya perlu dirawat dengan baik. Salah satu parameter gangguan kesehatan anjing adalah keberadaan ektoparasit dalam tubuh. Penelitian ini bertujuan untuk mengetahui prevalensi pada ektoparasit serta manajemen pemeliharaan anjing pemburu yang di pelihara di Kecamatan Jagong Jeget Kabupaten Aceh Tengah. Kegiatan penelitian telah dilaksanakan dari bulan November 2019 sampai bulan Februari 2020. Sampel yang digunakan dalam penelitian ini adalah 30 sampel anjing pemburu yang diperiksa dari pemilik anjing pemburu anggota PORBI dari Kecamatan Jagong Jeget dengan pemeriksaan ektoparasit secara makroskopis dan untuk manajemen pemeliharaan menggunakan instrumen kuesioner serta pengamatan. Data yang diperoleh dianalisis secara deskriptif.  Hasil pemeriksaan menunjukan bahwa prevalensi ektoparait pada anjing pemburu yang di pelihara di Kecamatan Jagong Jeget adalah 83,3%. Secara rinci ektoparasit dari kelompok kelompok Ctenocephalides canis yaitu 80%. diikuti oleh Rhipicephalus sanguineus dan Boophilus sp. dengan prevalensi 16,6% dan Trichodectes canis dengan prevalensi 3,3%. Dapat disimpulan bahwa terdapat infeksi ektoparasit pada anjing pemburu yang dipelihara di Kecamatan Jagong Jeget

    Deteksi Kebuntingan Ternak Sapi : Aplikasi Test Strip Dairy Cow Pregnancy Colloidal Gold Test Strip

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    Deteksi kebuntingan dini pada ternak sapi sangat penting ditinjau dari segi ekonomi karena akan mempengaruhi pendapatan peternak. Deteksi kebuntingan dini sangat penting untuk memperpendek calving interval melalui peningkatan pengetahuan peternak untuk mengidentifikasi status reproduksi, sehingga dapat melakukan terapi dan mengawinkannya sesegera mungkin. Kegiatan penetapan ternak sapi bunting atau tidak bunting dilaksanakan dengan metode deteksi kebuntingan. Penelitian ini bertujuan untuk mengetahui akurasi test strip dalam diagnosis kebuntingan pada ternak sapi. Test strip digunakan untuk mendeteksi kebuntingan 46 ekor ternak sapi Bali betina yang berasal dari peternakan rakyat.  Hasil menunjukkan bahwa test strip yang dilakukan pada 30 hari pasca inseminasi buatan menunjukkan sensitivitas 75% dan spesifisitas 90%. Test strip dapat digunakan untuk deteksi kebuntingan dini pada ternak sapi

    Identifikasi Mikroba dari Lendir Estrus dan Deteksi Kebuntingan Sapi Kawin Berulang di Sleman, Yogyakarta

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    Repeat breeding is condition of cows that have normal or near normal estrous cycles but fail to become pregnant after several insemination. In this study, we investigated the microbes in the cervical and vaginal mucus of beef cattle and dairy cows that experience repeat breeding and detected the pregnancy after the third or more artificial insemination. A total of 14 beef cattle and 6 dairy cows that experience repeat breeding in the cattle herd in the Yogyakarta region of Indonesia were used as samples in this study. Cervical and vaginal mucus samples were collected using plastic sheet when the cow was re-estrus. The samples were put into the 5 ml Brain Heart Infusion Broth for bacterial isolation and identification. Pregnancy examination was carried out on day 45 after artificial insemination using ultrasound method. Bacteria that could be isolated and identified from cervical mucus and vaginal mucus of beef cattle and dairy cows included Bacillus sp., Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas sp. Some cows detected positive in pregnancy examination even though the bacteria were presence in the cervical and/or vaginal mucus. There was various composition of bacteria found in the cervical mucus and vaginal mucus, of beef cattle and dairy cows with repeat breeding in livestock groups in Yogyakarta. The presence of bacterial in the cervical and vaginal mucus during estrus was not always become the causes of failed pregnancy

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