Jurnal Sain Veteriner
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In Vitro Anthelmintic Areca catechu Crude Aqueous Extract Against Haemonchus contortus in The Sheep
Get PDFHaemonchosis is a gastrointestinal parasitic disease in sheep caused by Haemonchus contortus. The biggest economic losses due to this disease are mortality, decreased production, stunted growth, and low body weight. The existence of resistance to synthetic anthelmintics causes the development of research on alternative treatments to herbal anthelmintics to be a strategic step. This study aims to analyze the effect of Areca catechu on Haemonchus contortus in vitro so that the Lethal Concentration 50 (LC 50) can be determined. This study was divided into 9 groups. Group I was treated with 2.5% A. catechu crude aqueous extract; group II was treated with 5% of A. catechu crude aqueous extract; group III was treated with 7.5% A. catechu crude aqueous extract; group IV was treated with 10% of A. catechu crude aqueous extract; group V was treated with 12.5% A. catechu crude aqueous extract; group VI was treated with 15% A. catechu crude aqueous extract; Group VII was treated with 17.5% A. catechu crude aqueous extract, group VIII was a negative control (0.9% NaCl) and group IX was a positive control (Albendazole). The mortality of H. contortus was recorded every hour until the worm mortality was 100%. The results were then analyzed using the Reed and Muench method. Based on the in vitro test of Areca catechu crude aqueous extract against H.contortus it can cause mortality in worms with an Lethal Concentration 50 (LC 50) calculation result of 11.11%
Pengaruh Pemberian Gonadotropin Releasing Hormone (GnRH) Terhadap Peningkatan Kualitas Spermatozoa Sapi Bali Pengaruh Pemberian Gonadotropin Releasing Hormone (GnRH) Terhadap Peningkatan Kualitas Spermatozoa Sapi Bali
No full textPenelitian ini bertujuan mengetahui pengaruh pemberian gonadotropin releasing hormone (GnRH) terhadap peningkatan kualitas spermatozoa sapi bali. Penelitian ini menggunakan dua ekor sapi bali berumur 2 dan 4 tahun dengan rancangan pola bujursangkar latin 2 perlakuan dan 2 periode perlakuan. Perlakuan yang dilakukan adalah P1 (NaCl fisiologis) dan P2 (GnRH 100 µg) dengan interval periode waktu perlakuan adalah satu minggu. Sampel semen dikoleksi menggunakan vagina buatan tiga hari setelah perlakuan. Data hasil pemeriksaan kualitas spermatozoa sapi bali dianalisis dengan uji Wilcoxon Signed Ranks Test. Berdasarkan uji statistik menunjukkan bahwa pemberian GnRH 100 µg tidak berpengaruh nyata (P>0,05) terhadap pemeriksaan warna, pH, konsistensi, volume, konsentrasi dan motilitas spermatozoa sapi bali. Hasil pemeriksaan menunjukkan bahwa warna semen pada kedua perlakuan adalah krem dan putih susu. Derajat keasaman (pH); konsistensi; volume semen (ml); konsentrasi (10⁶/ml); dan motilitas spermatozoa (%) sapi bali pada P1 vs P2 adalah 7,0±0,0 vs 7,0±0,0; 2,5±0,71 vs 3,0±0,0; 4,5±2,8 vs 4,8±2,8; 925±106,07 vs 1210±155,56; dan 69,0±1,41 vs 71,5±0,71 (P>0,05). Disimpulkan bahwa pemberian GnRH 100 µg tidak memengaruhi kualitas spermatozoa sapi bali
Perbandingan Perangkap Vavoua dan Nzi terhadap Keragaman dan Dinamika Populasi Stomoxys spp. pada Peternakan Sapi Perah di Pangalengan, Kabupaten Bandung
Get PDFStomoxys spp. merupakan salah satu vektor penting penularan penyakit yang disebabkan oleh berbagai virus, bakteri dan heminth. Infestasi Stomoxys calcitrans pada sapi perah dapat meningkatkan kortisol, menyebabkan gangguan makan, kegelisahan, hingga berkurangnya bobot badan dan produksi susu. Perangkap Vavoua dan Nzi dapat digunakan sebagai perangkap Stomoxys spp. yang mengalami fluktuasi populasi sepanjang tahun pada musim tertentu dan dipengaruhi oleh iklim, suhu, kelembaban, intensitas cahaya serta adanya zat atraktan. Tujuan penelitian ini adalah untuk membandingkan efikasi dan efektivitas perangkap Vavoua dan Nzi terhadap dinamika populasi dan keragaman spesies Stomoxys spp. yang terperangkap. Pengamatan dan pengambilan sampel dilakukan selama 12 jam (06.00-18.00) dengan menggunakan perangkap Vavoua dan Nzi yang dipasang berjarak 50 meter setiap perangkapnya, 3-5 meter dari kandang pada peternakan sapi perah di Pangalengan Kabupaten Bandung. Hasil penelitian menunjukkan bahwa terdapat tiga spesies yang dapat teridentifikasi selama Maret-November yaitu Stomoxys calcitrans (97,41%), Stomoxys sitiens (1,98%) dan Stomoxys indicus (0,60%). Puncak populasi Stomoxys spp. terjadi pada bulan September saat memasuki awal musim hujan dan puncak aktivitas harian Stomoxys spp. pada pukul 14.00-15.00 yang berhubungan dengan aktivitas di peternakan. Hasil uji normalitas data menggunakan uji Kolmogorov Smirnov dan uji homogenitas data memiliki nilai signifikansi p>0,05 sehingga dapat disimpulkan data berdistribusi dan bervariasi normal. Pada hasil uji t, didapatkan nilai signifikansi pada masing-masing parameter uji p<0,05 (0,005 dan 0,01), sehingga ada perbedaan penggunaan perangkap Vavoua dan Nzi terhadap jumlah lalat yang terperangkap
Study of Thitonia Diversifolia Extract in Histomorphology of Pancreas and Interleukin-1beta expression on Aloxan Induced Wistar Rats
Get PDFThe purpose of this study is to determine effect of antiinflammation of Thitonia diversifolia (TD) extracts in histopathology liver and pancretic tissue on alloxan-induced in Wistar rat. Thirty two male Wistar rats (Rattus norvegicus) with a body weight (bw)150-200 g in 3 month of age, were allocated into four groups, with eight animals per group. The control group received normal saline (P0), positive control group received CMC Na 0.01% (P1), the treatment group were received 100 mg/kg bw of the TD extracts (P2) and received 100 mg/kg bw of the metformin (P3) respectively for 7 days. Bloods serum collected for measuring of blood glucose (BG). cholesterol and insulin (INS) concentrations. The levels of insulin (INS) concentrations were analysed by Avidin-Horseradish Peroxidase (HRP) Sandwich-ELISA. All animal were sacrified for tissue staining with haematoxylin eosin. Thitonia diversifolia extract significantly decreased the level of blood glucoseand choelsterol concentrations compared to the postive control group (p<0.05). The level of INS could be detected by its level significantly increased in TD treatment group (p<0.05). Histopathological showed that induction of alloxan could effect severe multifocal degeneration, vacuolisation, cell inflammation, acute langerhan cells injury with destruction cells. Immunohistochemical staining labeled with IL-1β monoclonal antibodies (Mab) showed lowering expression of IL-1β reveal as brownish color aggregates on the langerhans cells in the TD treatment group. This study was concluded that TD extract is inhibited hepatic tissue injuries and decreased destruction in langerhan cells by decreasing IL-1β expression on alloxan-induced rats
TEPUNG BEKICOT (Achatina fulica) SEBAGAI PAKAN TAMBAHAN UNTUK MENINGKATKAN KUALITAS SEMEN DOMBA (Ovis aries)
No full textOne of the effort to provide superior rams is to pay attention to the provision of feed that has good substances and is able to improve the quality of sperm. Based on studies, snail flour has a fairly high protein content and snails can be an alternative source of protein in additional feed. This study aims to improve the quality of sheep sperm using an additional feed of snail flour (Achatina fulica) which can increase the use value and economic value. Eight sheep used in this research were divided into one group as the control and two groups as the treatment. The control group was given standard sheep forage, while the treatment groups were given standard sheep forage added with flour snail of 5% and 10%. Sperm collection using castration method. Parameters observed were motility, viability, and concentration of sperms. The results of the study showed that the addition of snail flour as additional feed for sheep affected sperm quality with a significant increase in concentration parameters, while motility and viability did not show a significant increas
Efek Pemberian Maserasi Taoge (Vigna radiata) terhadap Profil Hematologi dan Biokimia Darah Tikus Betina
Get PDFTaoge memiliki berbagai manfaat farmakologis yang berpotensi besar untuk meningkatkan kesehatan. Penelitian ini dilakukan untuk mengeksplorasi keamanan dari konsumsi maserasi taoge melalui pengamatan terhadap profil hematologi dan biokimia darah. Sebanyak 15 ekor tikus betina galur Sprague Dawley berumur 8 minggu, memiliki bobot badan 220-230 g, dan belum pernah bunting dikelompokkan secara acak ke dalam 3 kelompok perlakuan (n=5 tikus), yaitu kelompok kontrol (diberi air mineral); kelompok maserasi taoge 1%; dan kelompok maserasi taoge 5%. Pemberian maserasi dilakukan melalui air minum dan dilaksanakan selama 20 hari. Hasil menunjukkan bahwa secara umum perlakuan maserasi taoge tidak memberikan pengaruh nyata (P>0.05) terhadap profil hematologi dan biokimia darah tikus percobaan. Terdapat peningkatan signifikan (P>0.05) pada kadar hemoglobin pada kelompok maserasi taoge 1% dibandingkan dengan kontrol yang lebih tinggi dari nilai normal. Hal yang serupa juga terjadi pada nilai neutrofil yang lebih tinggi dari nilai normal namun tidak berbeda nyata dengan kontrol (p>0.05). Selain keduanya, semua parameter menunjukkan nilai yang tidak berbeda nyata dibandingkan dengan kelompok kontrol dan berada pada rentang nilai normal.. Hal ini mengindikasikan bahwa pemberian maserasi tauge tidak memberikan efek negatif dan toksik pada dosis 1 dan 5% terhadap profil darah serta fungsi organ hati dan ginjal
Effect of Purslane (Portulaca oleracea L.) Ethanol Extract in Alloxan-induced Hyperglycemic Rats (Rattus novergicus L.) Blood Glucose Levels
Get PDFDiabetes mellitus (DM) is a chronic condition which caused by insulin secretion disorder, insulin resistance or both. High number of diabetics per year has led many researchers to conduct research to find the most safe and effective medicine for DM treatment. Flavonoid are known to act as inhibitors of α-amylase as a parameter of pancreatic damage and help lower blood glucose levels. One source of flavonoids is purslane. The aim of this study was to examine the effect of purslane ethanol extract on glucose and α-amylase levels in rat. Twenty rats Rattus novergicus L. strain Wistar are divided into 5 groups; control, positive control, and three test groups, each was given purslane ethanol extract of 100 mg/kgBW, 200 mg/kgBW, and 300 mg/kgBW. Blood glucose and body weight were measured before alloxan induction, after induction, as well as day- 7, 14, 21, and 28; while feed and drink consumption were measured everyday starting with the administration of purslane ethanol extract. The α-amylase test was carried out on the 28th day using enzymatic UV determination of degradation product of maltose and glucose method. The analysis result showed that rats blood glucose and α-amylase decreased in each group with purslane extract. The antioxidant content of flavonoids in purslane ethanol extract has been shown to have anti-diabetic effects by repairing pancreatic function
Presence of Escherichia coli O157:H7 in Chicken Meat at Traditional Markets in Pangkalpinang City
No full textThis study was aimed to identify the presence of E. coli O157:H7 and analyze the association between factors and frequency of E. coli in chicken meat sold at Kaget Market, Pagi Market, and Pembangunan Market in Pangkalpinang City. A total of 30 chicken meat samples were collected proportionally using simple random sampling. This study was used a cross sectional method. The examination of E. coli was carried out based on SNI 2897:2008 concerning The Testing Method of Microbial Contamination in Meat, Eggs, and Milk and Their Processed Products. The E. coli O157 was confirmed with O157 latex agglutination test and H7 antiserum test. The data were analyzed using a descriptive test and risk factors were analyzed using chi-square and odds ratio. The results showed that E. coli O157:H7 was found in 2 of 3 samples (66,7%) isolated from Kaget Market. The significant risk factors (P<0,005) for the presence of E. coli were traders with age of more 45 years, kiosk distance of less than 5 meters from the highway, and washing hands using unclean water. The presence of E. coli O157:H7 will be a threat for consumer health
Diagnosis dan Pengobatan Babesiosis pada Anjing Shih-Tzu di Klinik Lilipoet, Yogyakarta, Indonesia
Get PDFDogs are one of the pet animals that have a loyal nature and are widely cared for in the community. It is not uncommon for dogs to often play in parks or outdoor environments that are surrounded by grass to play with their owners. The grass is a breeding ground for ticks, which can be vectors of disease carriers for dogs. Babesiosis is a disease caused by Babesia sp. and is transmitted by tick vectors. In this paper, we will discuss the clinical condition, diagnosis, and treatment of one dog with babesiosis at the Lilipoet clinic, Yogyakarta. A Shih-Tzu dog has clinical symptoms of fever with a temperature of 39.9oC, decreased appetite, and has a history of yellow vomiting, and found one tick. Blood was collected for microscopic examination of blood smears, blood hematologic, and molecular examination. Hematologic examination of the blood showed the value of thrombocytopenia with a value of 32 x 103/µL. Molecular examination with Polymerase Chain Reaction (PCR) showed positive results of Babesia sp. with visible DNA bands at 490bp. Treatment was carried out by giving one dose of antiprotozoal diminazen aceturate 3.5 mg/kg BW and doxycycline 10mg/kg BW once a day for 14 days
THE ANTHELMINTIC POTENTIAL OF TURMERIC (Curcuma domestica) EXTRACT AGAINST Ascaridia galli WORMS IN CHICKENS In Vivo
Get PDFAscaridia galli worm was chicken parasite that inflicted significant economic loss for breeders. Expensive commercial anthelmintic drug and drug resistance were the reasons why herbal anthelmintic study became strategic. The study aimed at finding out the effect of the application of turmeric (Curcuma domestica) extract in vivo on the number of eggs per gram (EPG) in feces. It used 30 free ranging chickens that were classified into 2 groups of 5 chickens with 3 repetitions. Group I served as control (Aquadestilata) and group II was treated using turmeric extract at the concentration of 2%, 3 times a week. Observation was conducted on days 1, 7, 14, 21, and 28. The results were then analyzed using Anova and advanced LSD test. The results of in vivo test showed that the application of the turmeric significantly decreased the number of the EPG as compared to negative control. The application of the turmeric had anthelmintic potential and significant effect on the decrease in the number of the EPG