Journal of Tropical Biodiversity and Biotechnology
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Diversity of Actinomycetes Isolated from Peat Soil of Undistrubed Forest and Pineapple Plantation in Sessang, Sarawak
Get PDFPeatland plays an important role not just as a carbon store but also in facilitating the flux of greenhouse gasses into the atmosphere. Apart from that, peatland is also home to a diverse population of microorganisms such as bacteria, fungi, and actinomycetes. Actinomycetes were known to be one of the most ubiquitous microbes that can be found in most of the soil types including peat soil. In this study, seventy isolates of actinomycetes were isolated from the peat soil using the soil dilution method. The 70 isolates of actinomycetes were later screened for their ability to produce secondary metabolites and antimicrobial activities using the agar diffusion method before the selected potential isolates were identified by targeting their 16S rRNA region. The results obtained showed 34.3% produce cellulase followed by, 12.8, 31.7, 80.0, and 51.4% for mannanase, xylanase, lipase, and protease respectively. The percentage of actinomycetes producing antimicrobial activity was 27.1 and 21.4% for Ralstonia solanacearum and Colletotrichum gleosporioides respectively. All the selected isolates of actinomycetes were identified as belonging to the genus of Streptomycetes spp. The potential actinomycetes were stored in freeze-dried form for future usage. This study showed that more diverse population of actinomycetes was obtained from the undisturbed forested peat soil area ecosystem compared to the agricultural peat soil area.
The Diversity of Ants (Hymenoptera: Formicidae) on Industrial Forest in Sungai Merah Village, Sarolangun, Jambi with Its Identification Key
Get PDFAnts have a very important role in an ecosystem. The insects act as decomposers, pollinators, soil aerators, pest controllers, and predators. Their role is very varied so that they can be easily found in various ecosystems, one of which is the rubber and oil palm ecosystem. The ant’s diversity in this forest eventually will affect the productivity of the land. The aim of this study is to determine the diversity and role of ants in the rubber and oil palm plantations in Sungai Merah Village. Both of the plantations dominate the industrial forest in Jambi. The method used in this study was purposive random sampling. Observation plots were installed in the ecosystem of rubber and oil palm plantations; each ecosystem had 4 plots consisting of 9 units of pitfall traps, and 9 units of bait traps. The results of this study show that 15 species of ants are found in the ecosystem of rubber and oil palm plantations. Furthermore, the collected ants consisted of 39.972 individual ants belonging to 12 genera and 5 subfamilies. Ants establish in the ecosystem of rubber and oil palm plantations in Sungai Merah Village have an important role either as predators (Crematogaster spp., Odontomachus rixosus, Odontoponera tranversa, Pheidole huberi, Tetraponera rufonigra, Tapinoma melanocephalum, Camponotus spp. and Colobopsis moeschi), or foragers such as ants from the genera Anoplolepis, Camponotus, Monomorium and Polyrhachis. As predators, Oecophylla smaragdina, Camponotus spp. and Crematogaster spp. also play a role as biological control agents in the ecosystem.
Diversity Status of Bamboo in Sumatra: A Review
Get PDFPrior to initiating the conservation effort, conducting a bamboo species inventory in a specific area is crucial for obtaining information about the diversity status of the species in that region. Species inventorying is a fundamental step in ensuring that conservation efforts are targeted, effective, and based on sound scientific data. Regrettably, status of bamboo diversity in Sumatra is unavailable. Therefore, a study was conducted to determine the status of bamboo diversity in Sumatra. The study gathered data from literature review. It covered bamboo species, their statuses (endemic, introduced, ex-situ conservation, and threatened), and encompassed the main Sumatra Island and surrounding smaller islands. Species were re-identified and validated. Conservation status was assessed based on IUCN categories. There are 73 species of bamboo in the Sumatra region, representing 10 genera: Bambusa (11 species), Chimonobambusa (1 species), Dendrocalamus (10 species), Dinochloa (2 species), Gigantochloa (26 species), Melocanna (1 species), Neololeba (1 species), Phyllostachys (1 species), Schizostachyum (19 species), and Thyrsostachys (1 species). Eighteen species of Sumatran bamboo are known as introduced species, and 30 species are endemic to Sumatra, with the majority belonging to Gigantochloa (15 species). The least represented are Bambusa and Dinochloa, each having one endemic species. Bambusa heterostachya, Dendrocalamus giganteus, and Dendrocalamus membranaceus fall into the “least concern” (LC) category on the IUCN Red List, while the remaining 70 species have not been evaluated. A total of 44 species have been planted (conserved) in botanical gardens in Indonesia, while the remaining 29 species have not yet been conserved
Edaphic Characteristics of Rafflesia Habitats in Indonesia: Implications for Conservation and Propagation
Get PDFRafflesia, a holoparasitic and endophytic plant, depends on its host, Tetrastigma spp., for survival, thus highlighting the critical interdependence between these species. Given the endangered status of Rafflesia due to anthropogenic pressures and narrow distribution, comprehensive conservation efforts are crucial. Ecological data on edaphic conditions, particularly the presence of the host, are important for effective conservation strategies. This study assessed soil properties across Rafflesia habitats on Sumatera, Borneo, and Java islands, revealing similarities in pH, carbon, nitrogen, cation exchange capacity (CEC), while the soil texture varied. These findings contribute valuable insights for informed conservation initiatives, both in-situ and ex-situ.
First Record of the Ladder Gudgeon Bostrychus scalaris Larson, 2008 (Gobiiformes: Eleotridae: Butinae) from Mangrove Estuary of South Sumatra, Indonesia
Get PDFA single specimen of Bostrychus scalaris was collected from the mangrove estuary of Banyuasin Regency, South Sumatra, Indonesia. The species was originally described as a new species based on a single specimen collected from the disturbed mangrove site in Sementa River, Selangor State, Malaysia. Subsequently, the species was known to have been distributed in Singapore based on a watercolour painting by a French naturalist also in Mekong River in Soc Trang Province, Vietnam. The present specimen of B. scalaris represents the first record of the species from Indonesian water and southernmost record of the species.
Inventory of Macrofungi in Area of Taman Hutan Raya (TAHURA) Ir. H. Djuanda Bandung
Get PDFTaman Hutan Raya (TAHURA) Ir. H. Djuanda Bandung is an integrated conservation area in which there are secondary natural forests that have various kinds of flora and fauna. Macrofungi are fungi whose fruiting bodies can be seen directly without the aid of a microscope, heterotrophic, and ecologically act as decomposers of organic matter (decomposers) and as biological control agents. Data related to research results on macrofungi in the Tahura area is still limited and need more comprehensive research. This study aimed to inventory, determine the growth factors, and potential utilization of macroscopic fungi. Observations were carried out during the period of November 2021 - April 2022 using exploration method. Sampling was done by purposive sampling method. Macrofungi identification process was carried out based on morphological characters such as cap, stalk, and lamella, The environmental factors observed included air humidity, soil pH, temperature, and type of substrate. The results showed that as many as 83 species were found in the Protection block, 50 species were found in the Collection block, and 99 species were identified in the Utilization block from the phyla Basidiomycota and Ascomycota. The macrofungi found have potential as biodegradation agents, food ingredients, non-food ingredients, drugs, antimicrobials, antioxidants, anticancer, and anti-inflammatory.
Bioerosion in the Late Eocene Discocyclina discus sowerbyi (Nuttall, 1926) in Bayat Area, Indonesia: Implications for Paleoecology
Get PDFThis paper discusses about the bioerosion discovered in carbonate tests of Discocyclina discus sowerbyi (Nuttall, 1926), a large benthic foraminifera from the Priabonian (Late Eocene). The study material was sampled from the Gamping beds in the Wungkal-Gamping Formation in Bayat, Indonesia. We discovered four bioerosional trace fossils from three different ichnogenera demonstrate bioerosion from the surface test analysis. Oichnus simplex and Oichnus paraboloides are ichnogenus Oichnus diagnostic drilling holes that are often found on the surface. Caulostrepsis isp. exhibits the presence of uncomplicated U-shaped borings. The observed formation of drill holes can be mostly linked to the predatory behaviour of gastropods, while other trace fossils are predominantly associated with the burrowing activities of worms. The occurrence of well-preserved individual tests exhibiting no signs of bioerosion is infrequently observed in D. discus sowerbyi. In addition, bioerosion occurs more frequently in the microspheric generation than in the megalospheric generation. This research also demonstrates for the first time in Indonesia that parrotfish bite marks have developed on individual tests of the microspheric generation of D. discus sowerbyi. The taphonomic characteristics exhibited by the bioeroded and encrusted D. discus sowerbyi specimens can serve as reliable paleoecological indicators for sediment deposition occurring at an intermediate to high sedimentation regime. The occurrence of larger foraminifera with some bioerosional trace fossil highly proficient at documenting shallow marine sclerobionts.
Astaxanthin Production from Green Microalga Haematococcus pluvialis under Various Bean Sprout Media Concentrations and Duration of UV Radiations
Get PDFAstaxanthin (AX) is known as a very strong antioxidant and has been utilised in many kinds of products such as foods, pharmaceutical, cosmetics, aquaculture, etc. One of the natural resources of AX is Haematococcus pluvialis which has been investigated by some researchers in order to enhance the AX production. However, the production of AX from the microalgae is still costly, hence, this present research is proposing low-cost methods namely bean sprout media (BSM) as an alternative growth media and UV radiation. The variations of BSM concentrations (2, 4, and 6 %) and times of UV radiation (1.5 and 3 hrs) were treated to H. pluvialis in laboratory conditions. BSM 4 % treatment showed an optimum growth of the microalga at 427 x 104 cell/ml (day 8) which also exhibited macrozooid, palmella, and aplanosore phases. UV radiation for 3 hr revealed that the concentration of AX production was as much as 17.37±0.04 mg/l. The research results were potential to be developed further in order to discover better and cheaper methods for scaling up AX production.
Cryptic Diversity of Barred Mudskippers, Periophthalmus argentilineatus (Valenciennes, 1837), from the Southern Coast of Java and East Lombok, Indonesia inferred by COI Mitochondrial Gene
Get PDFThe Barred Mudskipper (P. argentilineatus) is an amphibious fish species that displays fully terrestrial behaviour during low tides. Previous studies have indicated the existence of cryptic species of the barred mudskipper, leading to difficulties in taxonomic identification due to similarities in morphological characteristics. Therefore, this study aimed to generate DNA barcodes for Indonesian barred mudskipper populations. We collected ten specimens from Clungup Beach and Kondang Bandung Beach, representing our samples. Additionally, we incorporated 25 previously collected COI sequences from Indonesia into our analysis. The mitochondrial COI gene was amplified using PCR and analysed using various bioinformatics programs. This study provides evidence for the presence of three genetically distinct clades (A, B, and C) within the P. argentilineatus population in Indonesia, with a deep genetic divergence of 2.41% to 6.12%. Clade A showed a high genetic divergence of 5.51-6.12%, suggesting the presence of a cryptic species consistent with previous studies. The high level of haplotype diversity and low nucleotide diversity observed in each clade suggest a population bottleneck followed by a rapid expansion. The lack of geographical separation in the haplotype network analysis indicates that gene flow between populations may have been facilitated by glaciation events in the past. These findings contribute to a better understanding of the biodiversity of the barred mudskipper species in Indonesia and will aid in the accurate identification of cryptic species. This study highlights the importance of using molecular techniques to complement morphological identification in understanding the evolution and diversity of mudskipper fish species.
In Vitro Seed Germination and Shoot Growth of Nepenthes jamban Chi. C. Lee, Hernawati & Akhriadi, A Unique Pitcher Plant from Indonesia
Get PDFThe study to optimize in vitro propagation of the Indonesian native and critically endangered species, Nepenthes jamban, in order to support the ex-situ conservation efforts has been done. Using Murashige and Skoog (MS) as a basal media, disinfected seeds of N. jamban were germinated on five types of germination media, viz. ¼ MS, ½ MS, MS, ¼ MS+benzyl adenine (BA)+Biotin and MS+BA+Biotin. Afterwards, in vitro shoots with 6-7 leaves were inoculated on growing media, i.e., ¼ MS, ¼ MS 60 (3:1) (MS modification with a higher concentration of nitrogen), and ¼ MS+naphtalene acetic acid (NAA)+BA. The results showed that the germination of N. jamban seeds was slow, indicated by the percentage of germination being less than 20% after 6 months of being planted on germination media. The highest percentage of germination was after the 6th month and the greatest pitcher development at the 10th month were obtained on ¼ MS medium. Furthermore, shoot growth and pitchers development consistently increased for 12 months in ¼ MS 60 (3:1) medium while other media resulted in a decrease in pitcher formation. It seemed that low concentrations of nutrient in the medium proved to be more effective to induce in vitro seed germination and enhance shoot growth which was also supported by higher nitrogen (nitrate) concentration in the medium. This study provides information that supports ex situ conservation action of native and critically endangered Nepenthes species from Indonesia.