Journal of Tropical Biodiversity and Biotechnology
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Ectoparasite Infestation among Stray Cats around Surabaya Traditional Market, Indonesia
Get PDFThis study was conducted to determine the prevalence of ectoparasite infestation among stray cats around Surabaya traditional markets. A total of 305 stray cats were collected around 17 traditional markets in Surabaya City and were examined for the presence of fleas with a fine-toothed flea comb. Surveys were conducted during May-June 2019. 228 of 305 stray cats (74.75%) were infested with one species of ectoparasite. The average number of C. felis in every cat was 2.54, while the number of F. subrostratus in every cat was 0.33. Additional data about the gender, pregnancy/maternity, and bodyweight of every cat were recorded. The result of chi-square test shows that there is a significant difference between gender, pregnancy status, and bodyweight by the occurrence of ectoparasites (p=0.008; p=0.00; p=0.00). A total of 878 ectoparasites consisting of flea and lices, namely Ctenocephalides felis (88.27%) as the dominant ectoparasite, followed by Felicola subrostratus (11.73%). The highest infection rate (prevalence) of ectoparasite was found in Pucang Market (16.81%), while the lowest prevalence was found in Mulyorejo Market (0.8%). Coinfection was observed in only a few cats (1.63%). Multiple Regression showed that pregnancy is the most influential factor in the occurrence of fleas (p=0.000). These results should be taken into account among health workers to prevent a possible outbreak of zoonotic diseases caused by fleas.
The Plant Species Diversity of Lasitae Protected Nature Forest and Nearby Area, District of Barru, South Sulawesi
Get PDFAn expedition to Lasitae Protected Forest in District of Barru, South Sulawesi was undertaken to collect living plants for Pucak Botanic Garden, South Sulawesi. The aims were to investigate the diversity of the plants and as a dedication to the Expedition of the Republic of Indonesia Corridor Sulawesi. Using an explorative method, we collected the plants and recorded all data in the field following the tracks assisted by the field guide. A total of 179 collection-numbers have been collected from this dry lowland forest including the data for the local names. Many potential and valuable plants occur in the forest, 13 plants were highlighted in this paper for its conservation status, the potentiality for various purposes or its uniqueness: Diospyros celebica Bakh. or streak ebony (Ebenaceae), Arenga pinnata (Wurmb.) Merr. (Arecaceae), Phyllanthus lamprophyllus Mull.Arg. (Phyllanthaceae), Cycas rumphii Miq. (Cycadaceae), Lagerstroemia speciosa (L.) Pers. (Lythraceae), Garcinia celebica L. (Clusiaceae), Nervilia aragoana Gaud. (Orchidaceae), Phalaenopsis amabilis (L.) Blume (Orchidaceae), Ophioglossum reticulatum L. (Ophioglossaceae), Tetracera scandens (L.) Merr. (Dilleniaceae), Derris trifoliata Lour., Phytocrene bracteata Wall. (Icacinaceae), and Dioscorea hispida Dennst. Buchanania arborescens (Blume) Blume and Ardisia elliptica Thunb. can easily be found and widely spread
Plant Conservation Based on Tri Mandala Concept on Homegarden at Pakraman Penge Village, Baru Village, Marga District, Tabanan Regency, Bali
Get PDFPenge Pakraman village is a traditional village that has the potency to become ecotourism. The emergence of new tourist attractions affects land changes to support tourism activities. This causes the number of plant species in nature to decrease. Plant conservation by utilizing local wisdom is one effort to reduce the decrease of plant species number. The application of Tri Hita Karana and Tri Mandala in Balinese daily life able to support plant conservation activity. The objective of this research was to determine the role of Tri Mandala concept in plant conservation at Pakraman Penge home garden. Data collection methods are carried out through observation plant location with inventory number and name of plants in house sample. Plant use continues with study literature. The results of an inventory of plant diversity in home gardens of Pakraman Penge village recorded 70 species of plants from 16 houses sample. The plant habitus varied from herbs, shrubs until trees. Plant species in the home gardens have functions for ceremonies (51%), medicinal (24%), ornamental (17%), food (6%), and spices (2%). However, based on the location, most plant species were found in madya mandala and the lowest were found in utama mandala. We also found plants with conservation status consist of least concerned (15 species), vulnerable (Dracaena draco), near threatened (Cycas rumphii), endangered (Coffea arabica). Therefore, the application of Tri Mandala concept on Balinese home gardens supports plant conservation and gives economic benefit in individual leve
Diversity analysis of Rhacophorus margaritifer (Schlegel, 1837) in Baturraden based on RAPD markers
No full textRhacophorus margaritifer is an endemic species of arboreal frog in Java. Previous studies found this frog in several locations of Baturraden, namely in primary forest of Ketenger area, along Pancuran Pitu tourism track, and Baturraden Botanical Garden. There were still limited studies of molecular diversity of R. margaritifer and no prior data from population in the southern slope of Mount Slamet. This study aimed to look at the genetic polymorphism and determine the locus diversity of R.margaritifer population in Baturraden by using the PCR-RAPD technique. Frog tissue samples were taken from three populations in Kalipagu hiking trail (HPK 01), Baturraden hiking trail (HPK 02), and Baturraden Botanical Garden (KRB). DNA was extracted using the Chelex method. Molecular characterization was performed based on RAPD markers. The RAPD marker band pattern was changed to binary data 0-1 and analysed using Arlequin software ver.3.5. A total of 19 frog individuals were obtained during sampling at three locations. High genetic diversity had been observed in all populations with gene diversity range from 0.9643 in HPK 01 population to 1.0000 in both KRB and HPK 02 populations. A high locus variation was also observed for all populations with values of 0.159524 in KRB; 0.165816 in HPK 01; 0.192857 in HPK 02, respectively. AMOVA indicated no genetic difference among populations of R. margaritifer (p=0.50244).
RAPD Analysis for Genetic Variability Detection of Mutant Soybean (Glycine max (L.) Merr)
Get PDFThis study aimed to detect and evaluate the genetic mutation from mutagenized soybean by RAPD markers. Soybean seeds of “Grobogan” variety were treated with two different concentrations of EMS (0.5% and 1%) and three incubation times (4, 6 and 8 h). DNA whole-genome was isolated from young leaf seedling with the Qiagen DNeasy Plant Mini Kit. Twenty OPA primers (OPA-1 to OPA-20) were used for DNA amplification. The results showed that EMS treatments successfully generated genetic variation in soybean, which indicated by high values of PIC, EMR, and MI. RAPD primers that effective to detect the mutation were OPA-2, OPA-07, OPA-10, OPA-11, OPA-12, OPA-13, OPA-14, OPA-15, OPA-16, OPA-18 and OPA-20. The band expression of those primers was exhibited a stronger intensity along with increasing of EMS concentration and incubation time used in this study. Treatment of 0.5% EMS in 6 hours incubation was successfully generated soybean mutants with the lowest genetic similarity compared to the wild-type. Thus, this study provides a new approach to generate genetic variability in soybean and has the potential to improve for soybean breeding program
Considering Sampling Methods for Macrofungi Exploration in Turgo Tropical Forest Ecosystem
Get PDFDiversity of macrofungi is widely studied because of its role as decomposer, and highly reported of its potentially using for foods, agricultures, medicinal purposes, and bioremediation. This study was carried out to consider sampling methods for macrofungi exploration especially in Turgo tropical forest ecosystem. There were two sampling method models which adequately bring our macrofungi collection to have valid data. The first is adaptive sampling which includes fixed-size plots where prior we decided how many tracks, plots, or what size to examine at each site to help keeping our focus on track. By having a fixed plot, we allowed ourselves to find our macrofungi and work on the identification process thoroughly without racing against the time as much. Then we can obtain frequency, distribution, density and others ecological quantitative data by sampling plots. Another way, we can choose opportunistic sampling, we mean carefully walking on through chosen site as study area and collecting conspicuous sporocarp of macrofungi. This method needs no plots and does not produce any quantitative data but looking for many more diversity on macrofungi. The way of sampling, depends on the duration of observation and the purposes of the research. According to the consideration result, we concluded to choose the adaptive sampling method to explore the macrofungi in Turgo tropical forest ecosystem.
The Role of Sechium edule Fruits Ethanolic Extract in Insulin Production and Malondialdehyde Level in Stz-Induced Diabetic Rat
Get PDFThe objectives of this research were to examine the effect of Sechium edule ethanolic extract in insulin and Malondialdehyde (MDA) levels in streptozotocin (STZ) induced diabetic rats. Type 1 diabetic rat were obtained by intraperitonially injected with multiple low dose STZ (MLD-STZ) 20 mg/kgBW for 5 days. The animals were divided into 5 groups: untreated rats in group (K) were considered as negative control, MLD-STZ induced type 1 diabetic rats in group (S) were considered as positive control. In another 3 group (A, B, C) type 1 diabetic rats were orally treated with three doses of S. edule ethanolic extract (14, 28, 42 mg/kg BW) for 7 days. The animals were sacrificed in two days after last treatment, serum were collected for measuring of the insulin concentration by ELISA method. Level of MDA on kidney, liver and pancreas were measured by TBA method. The treatment with S. edule ethanolic extracts unable to increase of insulin yet but the treatment with 42 mg/kg BW of S. edule ethanolic extract showed significantly decreased MDA levels on liver but not significant on kidney and pancreas
Species Diversity and Potential Use of Asteraceae in Tawangmangu, Karanganyar Regency, Central Java
Get PDFAsteraceae is a group of plants with high species diversity, with about 25.000 species become members of this family worldwide. This family has many potential uses such as medicinal plants, ornamental plants and weeds. Tawangmangu is an area with unspoiled environmental conditions, where Asteraceae plants are found either wildly or deliberately cultivated in this area. This study aims to determine species diversity of Asteraceae and their potential use. This study was conducted in 5 villages namely Plumbon, Nglebak, Karanglo, Tawangmangu and Kalisoro. The samples were collected with random sampling method, while information on potential use of Asteraceae was gathered from literature studies and interviews with local people. Results of this study are expected to be basic data of species diversity as well as to provide reference of their potential use. A total of 32 species were found on various habitat, include roadside, home yard, riparian of river, in irrigation canal, and on agricultural landscape. The specimens found were all have potential as medicinal plants, with some wild plants were commonly known as weeds, many others were having potential as ornamental plants
Changes in Vegetation on Mount Agung Volcano Bali Indonesia
Get PDFVolcanic activity is a major natural disturbance that can catastrophically change an ecosystem over a short time scale. The eruption of Mt. Agung strato-volcano in 1963-1964 was considered among the most important volcanic event of the 20th century due to its effect on global climate. Studies on vegetation and landscape of Mt. Agung post-1970-1980 has been scarce. The current eruption of Mount Agung in June-July 2018, brought awareness of the importance urge to document the past and current landscape along with vegetation on Mt. Agung. Our study aimed to utilize remote sensing technique to explore the pattern of current (2017) land cover and vegetation density on Mt. Agung and estimate of vegetated areas and whether it has changed from the past. LANDSAT 8 images (www.earthexplorer.usgs.gov/) were used in this study. Supervised classification in ENVI was employed to obtain land use or land cover of the Mt. Agung area. Normalized Difference Vegetation Index (NDVI) was also calculated using the feature in the ARC GIS. Online web-based application, REMAP was used to obtain information on past and present condition of the crater of Mt. Agung to see whether there have been changes in vegetated areas around the crater using REMAP (www.remap-app.org). Results showed there are basically five main landcover that can be recognized namely forest (20758.23 ha), settlement (4058.37 ha), water area (41606.64 ha), open area (15335.64 ha) and farming (34554.78 ha). Our NDVI analysis also resulted in areas with have high density (78836.04 ha), medium density (15490.26 ha) and also no vegetation (31008.24 ha). Using web-based GIS application REMAP, we found that there has been an increase (approximately 1 km2) in vegetation cover from the 1980s to 2016. The changes in vegetation near the crater of Mt. Agung is relatively slow when compared to another volcano such as Mt. Merapi. Remote sensing application has enabled us to obtain information on vegetation change relatively easily compared to conduct an extensive on-ground survey where more time and funding is needed
Variations and Phenetic Analysis of Peanut Cultivars (Arachis hypogaea L.) Based on Morphological Characteristics
Get PDFPeanut (Arachis hypogaea L.) is a food commodity that is widely cultivated in Indonesia. At present there has been no analysis of the relationship between peanut cultivars with phenetic methods based on the morphological properties of the plants. Four cultivars of Arachis hypogaea L. Tuban, Talam 1, Talam 2, and Talam 3 used in this research. Morphological characters data was analyzed by description to construct identification key. Similarity index was counted by Simple Matching Coefficient (SSm) formula based on morphological scoring. Cluster analysis was conducted by UPGMA (Unweighted Pair Group Methods using Arithmetic Averages) method to construct dendrogram. PCA (Principal Component Analysis) were performed to defined role of each morphological character in grouping of accessions with MVSP (Multivariate Statistical Program) v. 3.1 software. The dendrogram showed that four cultivars of Arachis hypogaea L. divided into two main clusters, 4 sub-clusters. The similarity index of clusters is 0.85%