Journal of Tropical Biodiversity and Biotechnology
Not a member yet
327 research outputs found
Sort by
The Influence of Sex and Weather on the Activity Budget of Javan Slow Lorises (Nycticebus javanicus) in Garut Regency, West Java
Get PDFThe Javan slow loris (Nycticebus javanicus) is a nocturnal primate endemic to Java. Previous studies on slow loris activity are limited to general daily activity, and there is a lack of research on the potential sex differences in slow loris activity. This study aims to analyze differences in the daily activity of the Javan slow loris based on sex. From August to December 2018, the daily activity of six wild Javan slow lorises was recorded using behavioral observations with instantaneous point sampling at 5-minute intervals. Differences in male and female slow loris activity were analyzed using the Generalized Linear Mixed Model (GLMM). We set sex and weather as fixed factors and individuals as random effects. The results of this study showed that females spent more time feeding and less time resting than males. In addition, the Javan slow loris behavior was affected by temperature and humidity like other slow loris species
Growth of Kaffir Lime (Citrus hystrix DC) Cell Line Derived from Seed Explant After Yeast Elicitation Using Pure and Technical Grade Yeast
Get PDFThe addition of elicitors in kaffir lime (Citrus hystrix DC.) culture is one of strategies for obtaining and increasing the production of secondary metabolites. Saccharomyces cerevisiae is one of the elicitors that can be used to increase secondary metabolites such as terpenoids. However, in its use, the pure cultures of S. cerevisiae are expensive. Therefore, the first objective of this study was to analyze the ability of technical grade (commercial baker’s yeast) to be used as an elicitor and measure the growth of kaffir lime cell line after being elicited by pure and technical grade (commercial baker’s yeast). The second objective is to determine the best time to subculture kaffir lime cell line after elicitation. We observed the morphology and measured the growth curve of pure and technical grade yeast until the 4th subculture generation. Furthermore, we used both grades of yeast for elicitation. Kaffir lime cell suspension was treated with 10 ppm pure grade or 5 ppm and 10 ppm technical grade yeast for 4 days. After elicitation, kaffir lime cell lines were subcultured and their growth was analyzed. The result showed that the morphology and growth curve of technical grade until 4th subculture generations was similar to the pure grade. On the other hand, after elicitation using pure and technical grade yeast and being subcultured, the growth of the elicitated kaffir lime cell line had the same pattern as the control group, but the cell density of the control group was higher than the elicitated group. The initial stationary phase of kaffir lime cell line was on the 17th day which is the best time to subculture. The subculturing process is important to maintain the viability of the kaffir lime cell line.
Analysis of Soil Bacterial Diversity from Tropical Rainforest and Oil Palm Plantation In Jambi, Indonesia by 16S rRNA-DGGE Profiles
Get PDFOil palm plantations are the most invasive land use changes in Southeast Asia. It must have affected unique natural biodiversity. This study aimed to investigate the diversity of soil bacteria based on 16S rRNA gene profiles from tropical forest and oil palm plantation in Jambi Province, Indonesia. Soil sample was taken from tropical forest and oil palm plantation from Jambi province, Indonesia. The forest site is in Bukit Duabelas National Park, and the nearby oil palm plantation is in Sarolangun District, Jambi Province, Indonesia. The diversity of bacterial communities from topsoil was studied using Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene and common biodiversity indices. PCR amplification of 16S rRNA gene was successfully conducted primers-using 33F/ 518R primers. Phylogenetic approach was used for revealing the community shift of bacterial phyla and genera in both areas. Phylogenetic analysis showed there were 4 phyla of bacteria i.e., Firmicutes, Alphaproteobacteria, Gammaproteobacteria, and Actinobacteria, respectively. Actinobacteria was the most dominant group in both areas. The composition of soil bacterial community in the oil palm plantation, based on total number of bands 16S rRNA generated from DGGE was richer than that in the Bukit Dua Belas National Park. It was probably caused by plantation year circle more than 10 years and routine activities during the plantation management, such as applications of agricultural lime, herbicide and fertilizer.
Bycatch of Amboina Box Turtle (Cuora amboinensis) by Fishermen in Rawa Aopa, Southeast Sulawesi
Get PDFRawa Aopa is a permanent swamp ecosystem that serves as one of the suitable habitats for amboina box turtles (Cuora amboinensis). Some of the area is part of a national park (Rawa Aopa Watumohai National Park) and is designated as a traditional zone for fishing activities, where local fishermen sometimes reported accidental catch C. amboinensis in their fishing gear. The aims of this study were to record the existence of C. amboinensis bycatch, size and age structure of bycatch, characteristic of fishing activity, and to discuss the conservation implication of bycatch. The number of bycatch was recorded by direct observation of 7 selected fishermen in 14 days. The C. amboinensis caught accidentally were measured and weighed. A total of 38 individuals of C. amboinensis were accidentally caught by fishermen during the study, having a size ranged of 7.4 to 18.5 cm (juveniles, young adults, and old adults; no hatchling), and weighed 248 to 996 g. Based on sex, there was no significant difference between morphological size of male and female, although bycatch for females (59%) tend to be slightly higher than males (41%). Most of fishermen lives in Pewutaa Village and used bubu traps to catch fishes. C. amboinensis are the most common bycatch compared to other species. In order to minimize the impact of bycatch of the C. amboinensis by fishermen, we need to ensure that the turtle that accidentally trapped in the fishermen’s fishing gear would be released unharmedly to their habitat.
Morphological and Anatomical Variations among Alocasia alba Schott Accessions in Bali Botanic Garden
Get PDFAlocasia alba Schott is a member of Macrorrhizos group from Aroid family that has conserved in Bali Botanic Garden. On its development, the collections showed varied morphological diversity on leaves and flowers. The aim of this study is to fill the knowledge gap in morphology and anatomy of the species A. alba and to know the phenotypic variation in this species. A total of eight A. alba accessions from Java, Bali and West Nusa Tenggara were observed in morphological and anatomical characters. The result showed that the eight accessions of A. alba have some variations in morphological and anatomical characters. These variations might be caused by genetic factors that resulted from plant adaptation to the different environments.
Restriction Mapping of MC4R Gene on Bali Cattle (Bos sondaicus) as Genetic Marker for Breeding Program in Compared to Bos taurus and Bos indicus
Get PDFMC4R is a gene that has potential effects on growth traits such as body weight and feed intake. The usage of single nucleotide polymorphism (SNPs) in melanocortin-4 receptor (MC4R) as selection markers could help achieve effectiveness in the breeding program. This study aimed to analyze the restriction mapping based on SNPs in the MC4R gene for Bali cattle (Bos sondaicus) compared to various breeds of cattle. Partial MC4R gene was amplified using a primer (F: 5’-ACC AAT GTC AGT GAG TCC CC- 3’ and R: 5’-CTT CAT GTT GGC GCC CTG-3’) with a polymerase chain reaction (PCR) method. Genotype and allele frequencies were calculated using Chi-Square test and analyzed with Hardy–Weinberg law. Restriction enzyme was analyzed using Nebcutter V.2 to see the association between SNPs and the recognition site of restriction enzyme. The result showed four SNPs g.554 T>C, g.634 G>T, g.673 C>T, and g.742 G>A were found in the exon region. SNP g.742 G>A was found as a heterozygote genotype and the rest are SNP g.554 T>C, g.634 G>T, and g.673 C>T were found as homozygote genotypes. All SNPs were synonymous which did not change the amino acid translated. Three restriction enzymes were identified as MmeI, TspRI, and BsrI which attach to SNPs g.554 T>C, g.634 G>T, and g. 742 G>A respectively. SNPs found notably g. 742 G>A can be used as genetic markers associated with growth traits for further research on Bali cattle.
Morphometric Analysis of Sumatran, Kalimantan, and Javan Cyrtodactylus, which were Labelled as Cyrtodactylus marmoratus, Revealed Undescribed Species
Get PDFCyrtodactylus marmoratus is originally described based on the specimen from Java. Due to the marbled body colour pattern and the similarity in morphology, many specimens from outside of Java have been identified as C. marmoratus. The ongoing research, both molecular and morphological studies, showed that C. marmoratus is restricted to Java. The taxonomic status of specimens labelled as C. marmoratus from outside Java remains unresolved. Here, we study the morphometric and meristic of Javan, Sumatran, and Kalimantan Cyrtodactylus which were labelled as C. marmoratus to reveal their taxonomic status. We examined 11 morphometric and 19 meristic characters in 51 specimens at Museum Zoologicum Bogoriense (MZB) originally from, Java, Sumatra, and Kalimantan labelled as C. marmoratus as well as other recognized species. Principal Component Analysis (PCA) results show that C. marmoratus from Java differs from previous specimens which labelled as C. marmoratus from Sumatra and Kalimantan. The PCA results also show that C. marmoratus from Martabe differs from C. marmoratus Java which is supported by statistical analysis on interorbital, HeadW, HeadD, tubercle on the ventrolateral fold, dorsal tubercle and ventral scales. We hypothesized that Cyrtodactylus from Martabe is a suspected undescribed species-await formal description, and overall molecular analyses are needed for future study.
A Study on Diversity and Distribution of Figs (Ficus, Moraceae) in Bogor City, West Java, Indonesia
Get PDFFicus (Moraceae) is a keystone resource species in the tropical region, and it contributes significantly to Bogor City's vegetation composition. Ficus spp. provide habitat for urban animals and contribute to providing environmental services for the community. Minimum data distribution and increasing land-use change possibly decrease Ficus diversity in Bogor City. This study aimed to analyse the diversity and distribution of Ficus spp. in Bogor City. The research was conducted by dividing Bogor City into 128 plots sized 1 x 1 km. Relative abundance and distribution analysis used QGIS version 3.10.2-A Coruña. A total of 37 species of Ficus spp. from six subgenera were found in Bogor. The highest distribution is mainly located around the Bogor Botanic Gardens and the Ahmad Yani City Forest in a tree and hemiepiphyte. Ficus benjamina and Ficus septica were the most common species found and spread throughout Bogor City. The significant land-use change in Bogor City has resulted in Ficus spp. generally spread in the northern and central parts. In contrast, in the southern part, they are relatively low. Some Ficus can also be bioindicators because they have growing habitats that tend to be specific and spread in certain areas. The presence of the Bogor Botanic Gardens has an important role in increasing the diversity of Ficus spp. in Bogor City.
Evaluation of Bioactive Secondary Metabolites from Ponyfish Associated Bacteria (Photobacterium leiognathi)
Get PDFThe marine environment continues to surprise us by producing novel bioactive substances with a wide range of benefits for humans. Materials and Methods: Marine bioluminescent bacteria Photobacterium leiognathi was isolated from pony fish, Secutorruconius which was confirmed with microscopic and molecular characterization. The secondary metabolite of the isolated bacteria was extracted with dichloromethane. The chemical fingerprinting of the isolated metabolite was analyzed through TLC, FT-IR, and HPLC. The nature of the compound present in the metabolite was identified in the gas chromatography-mass spectrometry analysis (GC - MS). The isolated extract was investigated for its antibacterial property against 10 human pathogenic bacteria and also its antioxidant activity using different assays such as 1, 1-Diphenyl-2-picrylhydrazyl, Phosphomolybdenum, Metal chelating, Hydroxyl radical scavenging and hydrogen peroxide scavenging activity. Results: The Presence of functional groups including phenols, sugars, and amino acids in the extracts were identified by TLC. Totally, nine peaks were obtained for the crude extract through the FTIR spectrum range of 400 to 4000 cm-1 for the active sample. The DCM extract showed a broad spectrum of antibacterial activity against the six human bacterial pathogens. Secondary metabolites from the bioluminescent bacteria, P. leiognathi, have strong antioxidant properties. These results will be instrumental in developing novel products with biosensors and bio-imaging applications using P. leiognathi.
Reef Fish Diversity in Jayapura City, Indonesia: A Preliminary Study
Get PDFAs one of the marine areas included in the world's Coral Triangle region, Yos Sudarso Bay have a potential reef fish diversity that needs to be studied. However, there is very little information about reef fish diversity in these waters to date. This study aims to determine the species diversity of reef fish in Yos Sudarso Bay, Jayapura City, Indonesia. The study was conducted in April 2020 at seven sites; six of them located inside the Yos Sudarso Bay and one more located outside the bay. Sampling was carried out using the Underwater Visual Census method (25 m long and 5 m wide). Relative abundance by species, and diversity (H´), evenness (E), and dominance (C) indices were calculated. A total of 1,075 individual reef fish was recorded in seven study sites, representing 122 species and 26 families. Locations showed differences in reef fish abundance (86 to 215 individuals/125 m2), diversity (H´= 2.462 to 3.358), evenness (E = 0.770 to 0.887), and dominance (C = 0.047 to 0.155). This study has provided preliminary information on species diversity, fish abundance, and the ecological index of reef fish in Yos Sudarso Bay, Jayapura City.