Majalah Obat Tradisional
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Comparing Antioxidant Activity of Extracts and Gel Preparations Combination of Buas-buas Leaves (Premna serratifolia l.) and Secang Wood (Caesalpinia sappan)
Full text linkIndonesia is a tropical country that has exposure to ultraviolet rays from the sun throughout the year. This causes susceptibility to skin problems for the Indonesian population, including the vulnerability to aging of the skin due to exposure to ultraviolet rays for a long time. In the prevention of degenerative diseases and aging, antioxidants play an important role. Buas-buas and secang, which are plants from the West Kalimantan region, have very high antioxidant activity. Gel preparations are typically formulated as moisturizers with the aim of increasing water content in the stratum corneum, thus hydrating and maintaining skin pH. Hence, this study aimed to create a gel preparation combining buas-buas leaf extract and secang, effective as a moisturizing and anti-aging gel. The results of measuring the antioxidant activity of the combination of crude ethanol extracts of buas-buas leaves and secang wood with the results of the three formulas have very strong antioxidant activity, namely F1 at 0.0329 mg/ml, F2 at 0.0246 mg/ml, and F2 at 0.0246 mg/ml. F3 of 0.2282 mg/ml, and IC50 results for gel preparations for F1 3,8677 mg/ml, F2 4,3953 mg/ml, F3 4,396 mg/ml. The gel preparations produced in this study have the appearance and fulfill the evaluation requirements, except for the evaluation of spreadability
Skin Histopathology of Diabetes Mellitus Rats Treated with Edible Nest Swiftlets (Aerodhramus fuciphagus) Ointment
Full text linkThe edible nest swiftlets are suspected to contain sialic acid that can increase the process of cell growth that can reduce the formation of scar tissue and accelerate wound healing. This study aimed to determine the effect and the optimal dosage of edible nest swiftlets ointment on skin histopathology of diabetes mellitus rats that suffered a scratch wound. This study used 25 male white rats, divided into 5 groups, all groups were treated with betadine and the intervention of group 1 was vaseline only (control group), group 2, 3, and 4 were edible nest swiftlets (ENS) ointment with 10%, 20%, and 30% concentration and group 5 were the sanoskin as control positive. The rats were injured in the back area and given the treatment according to the group once a day for 14 days. Skin tissue was taken to make histopathologic preparations for observation on the 0th, 7th, and 14th days. The parameters observed included the number of macrophages, neo-capillarization, and fibroblasts. The result on the 14th day showed that the number of macrophages in the control group, sanoskin group, and ENS 10%, 20%, and 30% group was 3.8±5.019; 3.2±2.489; 1.84±2.387; 1.8±2.049 and 1.2±1.095. The result of neocapillilization were 1.28±1.673; 1.4±1.673; 2.8±1.778; 5.4±4.159; and 15±30.773. The conclusion showed there was a significant difference and there was the effect of edible nest swiftlets ointment on the number of macrophages, the number of neocapillilization, and the density of fibroblasts on the rats with diabetes mellitus that suffered a scratch wound
Identification of Compounds and Antidiabetic Activities of the Ethyl Acetate Fraction of Yacon (Smallanthus sonchifolius) Leaves using In Silico and In Vitro Approaches
Full text linkYacon (Smallanthus sonchifolius) is a plant that is vastly cultivated in Indonesia and has anti-diabetic activity. The purpose of this study was to isolate the compounds from the semipolar fraction of yacon leaves, as well as test them in silico and in vitro approaches. In this study, we use maceration for extraction, fractionation with n-hexane and ethyl acetate as solvent, and separation by preparative TLC method. The compounds tested were in silico by molecular docking using plant applications and in vitro by alpha-glucosidase inhibition assay. The difference between the molecular docking scores of an obtained molecule was calculated with Pair T-test methods. The results of the separation of the ethyl acetate fraction yielded a fraction, namely the H fraction. GC-MS analysis and IR spectroscopy showed that the H fraction contained the dominant compound called phthalic acid, di(2-propyl pentyl) ester. The docking score of the phthalic acid di(2-propyl pentyl) ester molecule and the alpha-glucosidase receptor, it is showed lower potency than 1-deoxynojirimycin as an alpha-glucosidase inhibitor. The in vitro test results showed that isolate H (IC50 = 130.479 ppm) from yacon leaves was no better than acarbose as an alpha-glucosidase enzyme inhibitor because the IC50 value was > 100 ppm
The Effect of Surfactant Concentration to Particle Size and Loading Dose of Immunity Jamu’s Ethanolic Extract SNEDDS (Self-Nano Emulsifying Drugs Delivery System)
Full text linkImmunity jamu consists of ginger, turmeric, Centella, and cinnamon, that act as immunostimulant agents. However, the infusion is impractical and used a limited dose of the extract, so it is necessary to develop drug delivery to resolve that problem. The SNEDDS technique is expected to increase the solubility, drug release, and absorption of active substances in the body, especially for low solubility of an active substance. SNEDDS consists of oil, surfactant, and co-surfactant. A surfactant is a substance that can reduce surface tension so that emulsion globules form in nanoparticle size. Tween 80 can produce a more transparent solution for oil-in-water emulsions than surfactants with low HLB values. This study aimed to determine the effect of surfactant concentration on the physical properties of SNEDDS to obtain the most loading dose but the smallest particle size. The formula consists of tamanu oil tween 80-propylene glycol of 1:7:1; 1:8:1; and 1:9:1 that incorporated extracts were 75, 150, and 375 mg. The physical tests included transmittance percentage, emulsification time on AGF media, phase separation, and stability test using the cycling test method. SNEDDS, then followed by the Particle Size Analyzer test. The results showed that the greater surfactant concentration produced a better transmittance value, a faster emulsification time, and stability. Formula with oil: surfactant: co-surfactant of 1:8:1 is a system that meets the requirement for immunity jamu with an optimal loading dose and small particle size compared to another formula. The extract’s loading dose of 375 mg has a particle size of 27.17 nm and a polydispersity index of 0.25
Antibacterial Activity of Cream, Ointment, and Emulgel of Ocimum basilicum L. Essential Oil against Propionibacterium acnes
Full text linkPropionibacterium acnes is bacteria that is commonly found on healthy human skin. However, unbalanced hormonal conditions can lead to excessive growth of P. acnes, a bacteria causing acne. Previous research reported that Ocimum basilicum L. essential oil (BEO) has activity against P. acnes. Therefore, this study aims to make BEO into cream, ointment, and emulgel forms to determine its properties and antibacterial activity on those topical drug delivery systems. The preparations consisted of variations in BEO concentrations of 5%; 10%; and 15%. The three preparations were evaluated based on organoleptic, homogeneity, pH value, adhesion, and spreadability. The antibacterial activity test of the three preparations against P. acnes was carried out by the good diffusion method with the observation of the diameter of the inhibition zone (in mm). The produced cream, ointment, and cream had a typical BEO aroma, yellowish white color, and homogeneous. Based on organoleptic consistency, the texture of the thicker and longer adhesion was ointment > emulgel > cream, respectively. The pH value of the topical preparation was 5-6 and easily leveled. Antibacterial activity against P. acnes from the strongest was ointment > cream > emulgel, with a value of 14,30 + 0,30 mm; 10,54 + 0,01 mm; 3,90 + 0,04 mm, respectively
The Effectiveness of Sungkai Leaf (Peronema canescens Jack.) Extract Gel on the Collagen Density of Incision Wounds in Vivo
Full text linkAn incision is an open wound resulting in skin injury and underlying tissues. The wound-healing process involves a proliferative phase that produces collagen. Sungkai leaves (Peronema canescens Jack.) can be used as an alternative therapy for wound healing because it contains flavonoids, tannins, and saponins that stimulate fibroblasts to synthesize collagen. This study aimed to determine the effectiveness of sungkai leaf extract gel in healing incision wounds in vivo based on collagen density scoring. Twenty-five Wistar rats (Rattus norvegicus) were randomly divided into five groups, the negative control group (K1) was given a gelling agent CMC 1%, the positive control group (K2) was given bioplacenton gel 1%, the treatment group (K3, K4, K5) was given a gel sungkai leaf extract concentration of 5, 10, and 15. The treatment is given topically at the incision site of the incision site of 0.1 g once a day for 14 days. The rats were euthanized on the fifteenth day, and the incision area was excised for histopathological preparation. The data obtained were analyzed using Kruskal-Wallis with SPSS 26. The test results showed p=0.010, meaning there is a significant difference (p<0.05) between the entire treatment. Based on these results, the K4 group (Sungkai leaf extract gel concentration of 10%) showed the highest collagen density scoring results compared to other treatments. This study concludes that sungkai leaves effectively heal incision wounds in Wistar rats based on collagen density scoring parameter
The Effect of Cultivation Site Altitudes on the Quality Parameters of Meniran (Phylanthus niruri L.)
Full text linkMeniran (Phyllanthus niruri L.) is known as a medicinal plant for immunomodulatory, hypoglycemic, diuretic treatment, and kidney disorders. It has phenolic and flavonoid as the active compounds. Several commercial products utilize meniran as the main ingredient for immunomodulators. However, the standard raw material for production has not been well reported. The study aimed to standardize the quality of meniran’s raw materials from different locations and altitudes. The phenolic compounds as the active component and their antioxidant activity were further studied. The raw materials were collected from Sukoharjo, Jember, and Karanganyar which are located from 104 masl, 243 masl, and 722 masl. The specific and non-specific standardization based on Indonesian Herbal Pharmacopoeia were investigated. The antioxidant activity was investigated. It resulted in the specific and non-specific parameters that were according to standard except for the acid soluble ash test from the Jember sample which was much higher (6.333% ± 0.969) than the standard (<1.2%). In addition, the flavonoid (1.529 ± 0.167%w/w) and phenolic (1.65 ± 0.006 %w/w) content showed that the sample collected from Jember was significantly higher than other locations. However, the strongest antioxidant activity was in the sample collected from Sukoharjo (IC50 101.84μg/mL). On the other hand, there was no correlation between the total flavonoid and the total phenolic to antioxidant activity in this plant. It suggested that the antioxidant activity in these plants did not depend on the flavonoid and phenolic compounds. In conclusion, the raw material of meniran (Phyllanthus niruri L.) collected from 3 different altitudes showed the quality of herbal medicine of mainly non-specific parameters and met the requirements of Indonesian Herbal Pharmacopoeia. The total phenolic and flavonoid as the active substances varied through all 3 different altitudes. It was in line with the antioxidant activity which varied to all 3 different altitudes due to the phytochemical profile variety
Phytochemical Constituents, Nutritional and Antibacterial Potentials of Selected Medicinal Plants (Allium sativum, Chromolaena odorata, and Talinum triangulare)
No full textAlternate medicine practices using natural plant extract may be of great importance in combating public health challenges. Medicinal plants are the bases for the development of modern drugs; accepted to treat infectious diseases globally. This study investigated the phytochemical constituents, proximate composition, and antibacterial activity of Allium sativum (garlic), Chromolaena odorata (Siam weed), and Talinum triangulare (Waterleaf) due to their reported medicinal properties. The phytochemical constituents and proximate composition were analysed using standard protocols. Agar diffusion technique was used to evaluate the antibacterial activities of the plants. All three plants included innumerable quantities of phytochemicals such as alkaloids, tannins, phlobatannins, saponins, flavonoids, anthraquinones, steroids, phenol, and cardiac glycoside. Proximate analysis indicated that the moisture (9.88%), fiber (4.57%), crude fat (2.89%), crude protein (3.67%), ash (3.78%), and carbohydrate (75.21%) present in Allium sativum; moisture (9.26%), fiber (15.28%), crude fat (3.56%), crude protein (18.86%), ash (11.76%), and carbohydrate (41.28%) present in Chromolaena odorata; moisture (10.37%), fiber (16.43%), crude fat (2.23%), crude protein (11.88%), ash (13.29%), and carbohydrate (45.80%) present in Talinum triangulare. The crude extract of Chromolaena odorata had the highest zone of inhibition against Pseudomonas aeruginosa (12.5mm). These bioactive substances, such as phenol, alkaloids, flavonoids, saponins, and tannins, augment the therapeutic characteristics of these plants, making them a common component of traditional remedies. They may serve as primes to drug discovery and be integrated into the health care delivery system. The investigations proved these plants' medicinal potential and further demonstrated Chromolaena odorata and Talinum triangulare's nutritional potential. They are good sources of income for individuals and bioprospecting
Physical Stability, Photostability, and Sunscreen Effectiveness of Combination Cream of Arabica Green Coffee Bean Extract (Coffea arabica) and Octyl Methoxycinnamate
Full text linkArabica green coffee beans (AGCB) have antioxidant compounds as photo protectors. It’s used to stabilize and increase the SPF value of octyl methoxycinnamate in sunscreen because its effectiveness will be decreased when exposed to UV light. The purpose of this study was to determine the effect of a combination of various concentrations of arabica green coffee bean (AGCB) extract and octyl methoxycinnamate (OMC) on the physical properties and SPF value of sunscreen cream. AGCB extract with concentrations (F1) 0%; (F2) 2.5%; (F3) 5%; (F4) 7.5% (with the addition of OMC concentration of 7.5%), and (F5) 7.5% (without the addition of OMC). The formulas were tested for physical properties to determine stability in 4 weeks of storage, cycling test for 4 cycles, and photostability to determine the SPF value and irritation test on the best formula. The results of the physical stability test on the five formulas showed a sig value > 0.05 which means the preparation is stable. Formulas F1, F2, F3, F4, and F5 with exposure of 1 hour, 5 hours, and 10 hours obtained a significance value > 0.05, which means that the presence of UV light exposure in the AGCB extract combination cream can stabilize the decrease in SPF value. Formula 4, which is stable without separation during the cycling test and the stability of the SPF value of 41, then in the irritation test does not cause irritation so it can be concluded that formula 4 is the optimum formula
The Ameliorative Effects of Phyllanthus gomphocarpus Hook. F. on Sperm Parameters of Rats Induced with Bisphenol A
Full text linkInfertility due to poor sperm quality is a common problem nowadays, particularly in developed countries. Herbs are one of the natural treatment options that have been proposed. Phyllanthus gomphocarpus Hook. F is a tropical plant that has traditionally been used in Malaysia to treat male infertility. Antioxidant characterization of its root aqueous extraction was performed using ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), total phenolic content (TPC), and total flavonoid content (TFC). Male rats were administered two different concentrations of the extract orally along with BPA for 21 days, and their sperm was evaluated using Computer-Assisted-Sperm-Analysis (CASA). The results showed that this plant has a high antioxidant capacity, with 78.6 ± 0.65 %, 0.14 ± 0.01 mg/ml, 28.52 ± 0.13 mg GAE/g and 164.73 ± 23.13 mg QE/g, for DPPH, FRAP, TPC and TFC respectively. The ability of this plant to enhance sperm concentration, motility, velocity, progression ratio, elongation, and percentages of normal sperm morphology significantly with p<0.05, appears to be due to its high antioxidant capacity. Furthermore, this plant was found to have sperm-protective effects against the endocrine disruptor BPA. A thorough investigation is required to determine the full mechanism of this plant's beneficial effects on male fertility and human health