Majalah Obat Tradisional
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Antioxidant Activity of Hydroalcoholic Extract of Puguntano Herbs and Andaliman Fruits by Cuprac Methods
Full text linkFree radical damage to the body can be prevented and repaired using antioxidants. Various natural ingredients native to Indonesia contain antioxidants, which are needed to improve people's health at an affordable cost. Puguntano (Picria fel-terrae) and Andaliman (Zanthoxylum acanthopodium DC.) are natural ingredients that can be used as antioxidants. This research determined the antioxidant activity and differences in IC50 values of hydroalcoholic extracts from Puguntano (Picria fel-terrae Lour) herbs and Andaliman (Zanthoxylum acanthopodium Fruits). Hydroalcoholic extracts were prepared by maceration using 90%, 80%, 70%, 60% and 50% ethanol. Antioxidant capacity was measured using the CUPRAC method, and quercetin was used as a positive control. The results were showed that the IC50 value for hydroalcoholic extracts of Puguntano herbs (27.02 ± 0.15 µg/mL, 25.94 ± 0.22 µg/mL, 25.12 ±0.15 µg/mL, 22.07 ± 0.23 µg/mL, and 23.86 ±0.18 µg/mL) and for Andaliman fruits (41.65 ± 0.30 µg/mL, 36.59 ± 0.40 µg/mL, 34.13 ± 0.44 µg/mL, 25.46 ± 0.30 µg/mL, 25.39 ± 0.30 µg/mL) respectively and quercetin 2.21 ± 0.02 µg/mL. Hydroalcoholic extracts of Puguntano herbs and Andaliman fruits have antioxidant activity, with each concentration having an IC50 of less than 50 µg/mL. The test sample's antioxidant activity is indicated by its lower IC50 value
Methyl Jasmonate Elicitation Enhanced Biomass, Phenolic, and Flavonoid Production of Adventitious Root Culture of Gynura procumbens in Balloon Type Bubble Bioreactor
Full text linkSecondary metabolites in the medicinal plant Gynura procumbens have various beneficial pharmacological activities. However, the limited availability of these beneficial compounds has constrained the possibilities for therapeutic applications. Exogenously applied methyl jasmonate (MeJa) as an elicitor has been reported to trigger the accumulation of secondary metabolites in plant tissues cultured in vitro. This study aimed to assess how varying periods of MeJa elicitation affect the growth and accumulation of phenolic and flavonoid compounds in adventitious root cultures of G. procumbens within a balloon-type bubble bioreactor (BTBB). Adventitious roots were induced from leaf and stem explants obtained from established in vitro shoots. Upon transfer to the BTBB, the cultures were supplemented with 100 µM MeJa and sampled at 1, 2, 3, and 4 weeks. The results revealed that including MeJa in the culture medium significantly boosted growth and the accumulation of phenolic and flavonoid compounds in G. procumbens. The highest biomass and concentrations of phenolic and flavonoid compounds were observed after 3 weeks of elicitation. These findings highlight the potential of MeJa elicitation for enhancing the production of valuable bioactive compounds in G. procumbens adventitious root cultures, showcasing its utility for industrial applications
Effect of Black Glutinous Rice Fermented Beverage on Short-Chain Fatty Acid Levels in Metabolic Syndrome Rats
Full text linkMetabolic Syndrome (MetS) represents a combination of metabolic factors that can elevate the likelihood of developing type 2 diabetes mellitus (T2DM) and coronary heart disease (CHD). The high prevalence of MetS results in a significant socio-economic burden. However, current management approaches have limitations, prompting the exploration of functional foods and nutraceuticals as promising alternatives. The primary objective of this study is to investigate the impact of Black Glutinous Rice Fermented Beverage (BGRFB) on Short Chain Fatty Acids (SCFA) concentration in a MetS rat model. Rats were induced with a high-fat diet (HFD) and streptozotocin (STZ)-nicotinamide (NA) to induce MetS conditions. BGRFB was administered as a treatment to the MetS rat group. The results showed that the Negative Control (NC) group exhibited a significant decrease in SCFA levels compared to the normal group (N). However, administration of BGRFB to the T2 group resulted in a significant increase in SCFA levels. The SCFA levels in the T2 group were higher but not significantly different from the Positive Control (PC) group treated with metformin. Increasing SCFA production could be an effective strategy in addressing Mets. This study demonstrates the potential of BGRFB as a therapy to enhance SCFA production and improve MetS. The outcomes of this investigation are anticipated to provide insights into novel dietary approaches for MetS management
The Hepatoprotective Effect of Moringa oleifera Leaves on Male Wistar Rat Induced Streptozotocin-Nicotinamide
Full text linkDiabetes mellitus is a disease characterized by uncontrolled increases in blood sugar levels, which can cause complications in the liver. Disorder liver disease can be seen from the increasing SGOT, SGPT activity, and the number of necrosis cells in the liver. Moringa leaves contain the flavonoid quercetin with antidiabetic and antioxidant activity. This research aimed to determine the effect of administering the ethyl acetate fraction of Moringa leaves on SGOT activity, SGPT, and liver histopathology in rat induced by streptozotocin and nicotinamide. Moringa leaves macerated with 80% ethanol and fractionated with ethyl acetate were used. The research subjects were 30 rats divided into six groups. The rats were induced with streptozotocin at a dose of 65 mg/kgBW and nicotinamide 100 mg/kgBW for five days, then treated for ten days. Serum SGOT and SGPT activity were measured using a Microlab 300 Semi-Automated at a wavelength of 340 nm. Histopathological observations were carried out when the rats were dislocated, their livers were taken, and then preparations were made using Hematoxylin Eosin (HE) staining. Data were analyzed using the SPSS One Way ANOVA method, followed by the Tukey test with a confidence level of 95%. The results of the study showed that administration of Ethyl Acetate Fraction of Moringa oleifera (EA-MO) could reduce the levels of SGOT, SGPT, and the number of liver necrosis cells in rats induced by streptozotocin and nicotinamide
Essential Oil Profiling and Antibacterial Activity of Curcuma xanthorrhiza Roxb. Originated from Yogyakarta by GC-MS
Full text linkAs a tropical country, Indonesia has a large diversity of flora and fauna, including numerous medicinal plants. Although herbs are widely used for medicinal purposes, their pharmacological effect often varies. This variability is frequently attributed to differences in the geographical locations where these plants are grown, leading to variations in the content of active metabolites. In this study, the profile of metabolite content in the essential oil of C. xanthorriza rhizomes was cultivated in three different regions in Yogyakarta, Indonesia, namely Mangunan, Ngawen, and Menoreh. Gas Chromatography-Mass Spectrometry (GC-MS) analysis was employed to assess the metabolite content of essential oil. At the same time, antibacterial activity against Escherichia coli and Staphylococcus aureus was evaluated using the microdilution method. Results indicated that the rhizome from Mangunan has the highest xanthorrhizol content with a percentage area of 21.07%. The Biplot on Principal Component Analysis (PCA) showed that the relationship of compound levels of the three essential oils has no similarity with one another. The Minimum Inhibitory Concentration at 50% (MIC-50) values of C. xanthorriza essential oils from Mangunan, Menoreh, and Ngawen regions were lower in inhibiting the growth of E. coli bacteria (1620.78 ppm; 1777.69 ppm; and 1688.39 ppm, respectively) compared to S. aureus bacteria (3080.80 ppm; 3340.14 ppm; and 2869.54 ppm, respectively)
Determination of Anticancer from EtOAc Fraction of Spatholobus Littoralis Hassk and of Molecular Structure Prediction
Full text linkIn recent years, the search for new anticancer drugs from local plants in Indonesia has become an interesting topic for research. One of the typical Indonesian local plants that have been used by the community as an anticancer traditional medicine (jamu) is Spatholobus littoralis Hassk (SLH) or better known as Bajakah. The native Dayak people of Kalimantan have used it for years for the treatment of breast cancer. In this study, the separation of single compounds from the ethyl acetate fraction of SLH stems was carried out, their molecular structure was analyzed, and anticancer bioactivity was tested. The isolation used the preparative HPLC method. The elucidation of the isolates from the ethyl acetat fraction used LCMS-MS, FTIR, and NMR. Anticancer bioactivity was determined using the MTT bioassay. The result is the acquisition of two isolates; it has been predicted that both are pseudobaptinin. The anticancer IC50 of the pseudobaptinin was 62.85 ppm, and Pseudobaptinin with impurities was 145.60 ppm
Using Macroscopic, Microscopic, and FTIR Spectroscopy combined with Chemometrics to Authenticate Arabica Coffee from Antbush
Full text linkLimited coffee stocks, high consumption, export demands, and increasing prices may contribute to counterfeiting. In other countries, including India and Africa, coffee forgery using antbush has been extensively established. Due to its abundance in Indonesia, unscrupulous coffee growers allegedly employ antbush (Senna occidentalis) as an adulterant in coffee to increase commercial earnings. This study aims to authenticate coffee from antbush using macroscopic and microscopic differences and FTIR spectroscopy combined with chemometrics. The arabica coffee samples from various regions were oven-roasted to a second crack and milled. The materials were then examined under macroscopic, microscopic, and infrared spectroscopic conditions. The obtained responses were used to monitor the qualitative and quantitative information in the targeted samples. Antbush in coffee samples is successfully identified microscopically by remnants of palisade tissue as well as the structural differences of the endosperm. In addition, FTIR spectroscopy combined with multivariate calibration can accurately estimate the concentration of antbush as adulterants in the target sample. Principle component regression (PCR) provides the best modeling for the relationship between the actual value. FTIR predicted the value of antbush with the lowest RMSEC and RMSEP values of 0.852 and 0.896, respectively, with the coefficient of determination (R2) in calibration and validation models of 0.9996 and 0.9967, respectively. The combination of macroscopic, microscopic, and FTIR spectroscopy offered reliable tools to authenticate arabica coffee from antbush
Ursolic Acid and Polydatin in Melinjo Seeds Inhibit AKT1 and GAPDH Protein and HTB-179 Cells Migration
Full text linkThe incidence of lung cancer in Indonesia by 2020 has reached 34.783 cases. Melinjo contains ursolic acid and polydatin, which can suppress cell proliferation and induce cell apoptosis, respectively. This study aims to determine the Melinjo seed ethyl acetate fraction (MSEAF) ability to inhibit lung cancer proliferation and migration towards HTB-179 cells using in vitro and in silico methods. Melinjo seed powder was macerated using 70% ethanol and fractionated with ethyl acetate. The fraction obtained was then analyzed using HPLC to detect the active compounds. The compounds obtained were further analyzed using bioinformatics to determine the target proteins. The docking method was performed between ursolic acid and polydatin compounds with each target protein to determine the binding affinity. The in vitro test was done using the MTT cytotoxicity assay and scratch wound healing assay methods. The results showed that MSEAF contains ursolic acid and polydatin with retention times of 12,475 minutes and 16,564 minutes, respectively. Ursolic acid protein targets were TP53 and AKT1 with docking scores of -6,3 kcal/mol and -7,4 kcal/mol, while polydatin target proteins were GAPDH and VEGFA with docking scores of -8,8 kcal/mol and -5,5 kcal/mol. The results of the MTT assay showed an IC50 value of 35,539 g/mL, and MSEAF inhibited the migration of HTB-179 cells by slowing the migration rate. This study suggested that the MSEAF contained ursolic acid and polydatin, which exhibited the ability to prevent the growth and migration of HTB-179 lung cancer, supported by the prediction of their ability to bind to TP53 and AKT1 proteins
Analysis of Diclofenac Sodium in Traditional Medicine (Jamu) for Rheumatism in Banjarmasin using TLC-UV-Vis Spectrophotometric Method
Full text linkJamu is one of Indonesia's most consumed traditional medicines. The government has clearly stated that traditional medicines are strictly prohibited from containing chemicals, both isolated and synthetic. Diclofenac sodium is one of the Non-Steroid Anti Inflammation Drug (NSAID) class drugs that have an effect as a pain reliever and anti-inflammatory, which is also often misused in traditional medicine preparations. The purpose of this study is to determine whether there is a chemical content of diclofenac sodium contained in the preparation of jamu pegal linu circulating in Banjarmasin using thin-layer chromatography (TLC) and to determine whether the validation of the method using a UV-Vis spectrophotometer meets the established requirements. The results of qualitative analysis using thin-layer chromatography showed that 4 out of 7 samples identified positively contained diclofenac sodium with an Rf value of 0.825. The results of quantitative testing using a UV-Vis spectrophotometer showed the acquisition of an r-value of 0.9996, %RSD of 0.97%, % recovery of 95.28%, 92.82%, and 95.48% for the addition of standard solutions of 10 ppm, 15 ppm, and 20 ppm, as well as LOD & LOQ values of 1.1625 ppm and 3.8750 ppm. Based on the analysis results, it can be stated that 4 out of 7 samples of jamu pegal linu circulating in the Banjarmasin area are positive for diclofenac sodium with levels ranging from 10.257 - 18.465 mg/g (1.026% - 1.846%)
The Development of Antioxidant Nutraceuticals containing Chrysanthemum indicum L. Gummy Candy
Full text linkChrysanthemum flowers (Chrysanthemum indicum L.) have been shown to contain flavonoid compounds and exert antioxidant activity. This brings Chrysanthemum indicum potential to be developed as a nutraceutical product. This study aims to evaluate the antioxidant activity of Chrysanthemum flowers before and after the formulation of Chrysanthemum indicum flower extract as a gummy candy. Gummy candy formulas were developed using variations of gelatin and pectin as gelling agents. In this study, Chrysanthemum flower extract was formulated into gummy candy. The physical characteristics evaluated include organoleptic tests, weight uniformity, elasticity, and moisture content. Optimization was performed using the simplex lattice design (SLD) method with the aid of the Design Expert software Ver. 13. The antioxidant activity of the chrysanthemum flower extract and gummy candy extract was evaluated using the DPPH radical scavenging method. Ascorbic acid was used as a positive control. The optimum formula for preparing the gummy candy was 11.51% of gelatin and 1.24% of pectin. The evaluation of weight uniformity, elasticity, and moisture content suggested that there is no significant difference between the optimum formula and the predicted value. Both the chrysanthemum flower extract and chrysanthemum flower gummy candy had strong antioxidant activity. The IC50 value of the extract was 67.80 ± 2.37 mg/mL while the gummy candy IC50 value was 82.93 ± 2.55 mg/mL. The antioxidant activity of Chrysanthemum indicum was slightly decreased after being formulated into gummy candy. These studies suggested that scientists are expected to anticipate the decrease of Chrysanthemum antioxidant activity in the gummy manufacturing process