Majalah Obat Tradisional
Not a member yet
427 research outputs found
Sort by
The Effect of Papaya Seed Ethanol Extract in Vivo on The Number of Osteoblasts Cells of Periodontitis-Induced Rats
Get PDFThe papaya seed ethanol extract is rich in antioxidant ingredients, such as flavonoids and phenolic acids. One of the main factors causing chronic inflammatory in periodontitis is oxidative stress. Administration of papaya seed extract is assumed to increase the number of rat osteoblast cells induced periodontitis. This research was conducted to analyze the effect of papaya seed extract on osteoblasts cells of rat induced periodontitis. This research represented an experimental laboratory-based investigation involving 35 rats of the Rattus norvegicus strain divided into 5 treatment groups (K, P1, P2, P3, P4). Control group (K) was not induced by periodontitis and was not given an extract, while group P induced periodontitis using LPS Phorphyromonas gingivalis (P.gingivalis) for 7 days and continued wire mesh installation around the mandibular incisors in the form of number “8” for 7 days. P1 group was given feed only without extract, while the rest were given extract of 200 mg/kgBB, 300mg/kgBB, and 400mg/kgBB. The data obtained were analyzed with a one-way ANOVA test. The results showed that the average number of osteoblasts varied significantly between the groups. There was an increase in the average number of osteoblast cells in rat induced periodontitis after given papaya seed extract
The Antimalarial Activity of the Water Extract of Simpur (Dillenia Indica L) Leaves against Plasmodium Berghei in Mice
Get PDFMalaria, caused by the climate of the subtropical area and the forest with many rivers and immovable water, is a contagious disease that still becomes a health problem in West Kalimantan. Simpur is a plant that is used by the locals to cure malaria. Therefore, this research aims to study the antimalarial activity in vivo and the 50% inhibitory concentration (IC50) of the water extract of Simpur leaves (Dillenia indica L) against Plasmodium berghei. This research used Peter Test method that used 7 test groups based on the test solution namely positive control group that was given dihydroartemisinin+piperaquine (DHP), negative control that was given aquabidestilata and the test group that was given the water extract of Simpur leaves with various doses of 20, 40, 60, 80 and 100 mg/Kg BB of mice, which each group was given the test solution for 3 days. The result shows that the water extract of Simpur leaves could lower the parasitemia count with IC50 19,22 mg/kg BB
Metabolomic Study of Three Species in Zingiberaceae Family based on 1H-NMR
Get PDFZingiberaceae is an economical plant and widely used as traditional medicines. Its rhizomes have been reported to have biological effects due to their metabolites content. However, metabolites profiling in Zingiberaceae has been little reported comprehensively. Therefore, this study aimed to profile primary and secondary metabolite of three species Zingiberaceae rhizomes (Zingiber amaricans BI, Zingiber officinale Roscoe, and Alpinia purpurata (Vieill.) K.Schum using 1H-NMR-based metabolomic approach. All samples were collected from local farmer located in Nguter, Sukoharjo, Central Java. Multivariate statistical analysis and ANOVA applied to measure the differences. It resulted that metabolite profiling discriminated between Zingiber and Alpinia samples. Fructose, α-glucose, β-glucose, sucrose, malic acid, alanine, valine, and shogaol contributed in discrimination between Z. amaricans BI, Z. officinale Roscoe, and A. purpurata (Vieill.) K.Schum. Sugar (α-glucose, β-glucose, fructose, and sucrose) and malic acid were significantly higher in Alpinia than in Zingiber samples. Relative concentration of amino acids (alanine and valine) and shogaol were significantly higher in Z. officinale. This result might be useful for databases and supplementary informations in Z. amaricans, Z. officinale, and A. purpurata taxonomy classifications
Hair Growth Promoting Activity of Green Tea Leaves (Camellia sinensis l.) Ethanolic Extract
Get PDFHair loss and baldness is one of the abnormalities in the hair that often occurs in both men and women. Many cosmetic products from natural to synthetic materials have been developed to overcome these problems, yet, synthetics product is potential to give side effects, such as local irritation. In this study, green tea (Camellia sinensis L.) leaves were used as active substances. The aim of this study was to attest the hair growth promoting activity of n-hexane, water, and ethyl acetate fractions from ethanolic extract of green tea leaves. Green tea leaves contain flavonoids which can help promoting hair growth. To obtain the compound, green tea leaves were made into ethanolic extract. The extract was obtained by maceration using 70% ethanol then partitioned using n-hexane, ethyl acetate, and water, to obtain the n-hexane, ethyl acetate, and water fractions. Ethyl acetate and water fractions have been shown to contain flavonoids, so it could continue the hair growth promoting activities with concentrations of 1% and 4%. Hair growth promoting activity was performed on rabbits. The results showed that 4% of water fraction containing flavonoids had the best hair growth promoting activity
Metabolite Fingerprinting Eleutherine palmifolia (L.) Merr. Using UPLC-QTOF-MS/MS
Get PDFEleutherine palmifolia (L.) Merr. (E. palmifolia) is an Indonesian native plant that has the potential to be developed into phytopharmaca. The differences in growth locations are thought to cause variation in the content of metabolite compounds which affect differences in pharmacological activity. This study aims to determine the profile of metabolites E. palmifolia bulb from several regions in Indonesia. The samples were collected from six different locations, namely East Java, Central Java, West Java, East Borneo, Central Borneo, and South Borneo. Sample extraction was carried out using Ultrasonic Assisted Extraction (UAE) method with 96% ethanol. The analysis of the content of metabolites was carried out using UPLC-QTOF-MS/MS with a stationary phase column C18 (Okta Decyl Silica), mobile phase mixture of formic acid /water 0.1/99.9 (v/v), and formic acid/acetonitrile 0,1/99,9 (v/v). The results of the analysis were interpreted using software Masslynx and continued with chemometric analysis using the method Principle Component Analysis (PCA). The results showed that there were differences in the content of the metabolite compounds in E. palmifolia bulb originating from six different regions
Isolation and Identification of DPPH Radical (2,2-diphenyl-1-pikrylhidrazyl) Scavenging Active Compound in Ethyl acetat fraction of Piper acre Blume
Get PDFPiper acre Blume, known as Black Betel (local name), is a plant that is widely used by the people of East Kalimantan, especially in Samarinda, for the treatment of illness. Leaves (3-4 months old) are collected from Samarinda, extracted, fractionated, and monitored by DPPH antiradical activity. The isolation of the Piper acre Blume is performed on the active fraction, and the structure identification is based on spectroscopic data of the compound. The leaves were dried, pulverized, and macerated with MeOH. Dried MeOH extract was obtained upon evaporation of the solvent. The extract was then fractionated by vacuum liquid chromatography (vlc), eluted gradually by solvents having different polarities (n-hexane, ethyl acetate and methanol). The fractions obtained were monitored using TLC [n-hexane: ethyl acetate (3: 1 v/v)] that was visualized by UV254 nm, UV366 nm and DPPH. The isolation was performed by preparative TLC [SiO2, n-hexane: ethyl acetate (3: 1)] on ethyl acetate fraction that showed the highest DPPH antiradical value. A single compound was obtained, and it appeared as a round spot and pure according to TLC performances at 3 different solvent systems. The isolated Piper acre Blume compound displayed the IC50 value on the anti-radical DPPH (measured at λ 520 nm) as 10.41µg/mL. The IR spectrum (KBr) showed –OH band (3450 cm-1), aliphatic bands [alkene, 3010 cm-1; alkana 2900 cm-1), an aromatic overtone bands (1900-200 cm-1) and a strong C=O band (1725 cm-1). The NMR (1H- and 13C-) (mono and 2D) indicated the present of a p-di-substituted aromatic signals (δ, 7.54 and 7.52, d, J =6 Hz, 1 H each), 2 methyl (δ, 0.96, d, J = 7.0 Hz, 6 Hs), a triplet signal (δ, 4.22 ppm). Other signals of CH- and CH2 were shown as m signals at δ, 1.64 and 1.34 ppm. Based on those data, the compound was identified as isoamyl p-OH benzoate that is grouped as parabens used as a preservative in the pharmaceutical preparations. In conclusion, the anti-radical (DPPH) active compound present in the leaves of Piper acre Blume is identified as isoamyl p-OH benzoate, having IC50 value anti-radical DPPH 10,41µg/mL
Influence of Some Extraction Conditions Factor on Phenolic Content and Antioxidant Activity of Solanum betaceum Cav.
Get PDFSolanum betaceum Cav. fruit is renowned for having antioxidant activity because it contains phenolic compounds. This study aimed to determine the effect of some condition factor namely solvent composition, maceration time, liquid-solid ratio, and the particle size of S. betaceum Cav fruit to the total phenolic content and antioxidant activity. The fruit was collected from Temanggung, Wonosobo, and Kopeng, Central Java, Indonesia. The research used single-factor experiments and simplex lattice design (SLD) as an optimization method. Total phenolic content was determined using Folin Ciocalteau reagent, while antioxidant activity was determined using 2,2-diphenyl-1-picrilhidrazil (DPPH) radical scavenger activity. The solvent combination which gave the highest responses was ethanol: water (60:40 v/v) with phenolic content of 7.48% w/w EAG. Maceration for 8 hours will produce an extract with the highest total phenolic content (8.76% w/w EAG). The optimal solvent ratio was at 10:1 v/w with total phenolic content of 7.26 ± 0.20% w/w EAG. The optimum particle size was 600-850 μm with a total phenolic content of 6.07 ± 0.18% w/w EAG. Antioxidant activity with the DPPH free radical scavenger capture method from three regions did not show significant results
Determination of Total Xanthone Content in the Preparation of Mangosteen Pericarp Capsules (garcinia mangostana l.) Available on the Market using UV-Visible Spectrophotometry Method
Get PDFThe skin of Mangosteen fruit (Garcinia mangostana L.) is widely used as traditional medicine, for it contains a lot of xanthone compounds. Today, there are many products from mangosteen pericarp extract being sold on the market, one of which is the preparation of mangosteen pericarp capsules. Determining the total xanthone content in the preparation of mangosteen pericarp capsules (Garcinia mangostana L.) circulating on the market needs to be done to calculate the levels of xanthone that are not included in the preparation label. The results of the analysis with UV-Visible Spectrophotometry obtains the maximum absorption wavelength at 243 nm and the linear regression equation of the calibration curve that is y=0,0568+0,0727x with a value of r=0,9999. Validation of the analysis method showed the results of intraday precision obtained % RSD 0,34%, 0,17%, and 0,16%, interday precision of 0,23%, 0,35%, and 0,16%. The accuracy obtained was mean % recovery 95,55%, limit of detection 0,35 μg/ml and limit of quantitation 1,15 μg/ml. The results of the determination of the total xanthone content in the mangosteen pericarp capsules were 100,8 μg/mg in sample A; 197 μg/mg in sample B; and 50,2 μg/mg in sample C
The Inhibition Activity of Tannin on the Formation of Mono-Species and Polymicrobial Biofilm Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans
Get PDFBiofilm acts as the mediator for infection nowadays. Approximately, more than 80% infection incidents are biofilm-formation related. Biofilm as bacteria's defense system is more difficult to eradicate by antibiotic; therefore, pathogen bacteria on their biofilm forms can make serious problems for human health. The invention of a new candidate for polymicrobial biofilm can be an essential challenge to be studied, in order to prevent infections related to biofilm. Tannin is a polyphenol compound with anti-bacterial and anti-fungal potential. This study aims to acknowledge the effectiveness of tannin in inhibition and degradation of C. albicans, P. aeruginosa, E. coli, S. aureus, and polymicrobial biofilm. The assay for biofilm inhibition and degradation were determined with microtiter broth method. The effectivity of tannin antibiofilm against polymicrobial biofilm were analyzed by calculating minimum biofilm inhibitory concentration (MBIC50) and minimum biofilm eradication concentration (MBEC50) values. The mechanism of action of tannin against polymicrobial biofilm was tested using scanning electron microscopy (SEM). The data were analyzed using the Statistical Package for the Social Sciences (SPSS) with a 95% confidence level. Tannin 1% gave inhibition activity of mono-species biofilm formation S. aureus in the middle phase and maturation of 79.04±0.01, 61.48±0.03, E. coli 74.56±0.01, 67.91±0.02, P. aeruginosa 67.32±0.05, 35.13± 0.01, C. albicans 60.62±0.01, 47.16±0.01. The results also provide evidence that tannin activity can degrade and damage the matrix of extracellular polymeric substance (EPS) polymicrobial biofilms. Hence, tannins can be a potential candidate for new antibiofilm for polymicrobial biofilm
Ethanol Extract of Detam 1 Soybean Seed (Glycine Max L. Merr) for Chronic Kidney Disease Therapy by In Vitro Study
Get PDFChronic Kidney Disease (CKD) has increased incidence and prevalence in developing nations. In this in vitro study, we evaluated the cells proliferative effects, fibronectin (FN), transforming growth factor β (TGF-β1), and Reactive oxygen species (ROS) - level inhibition potential of ethanol extract of detam 1 soybean seed (EEDS) on glucose-induced kidney mesangial cells (SV40 MES 13). The cells proliferation assay used 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)2H-tetrazolium (MTS) assay. FN and TGF-β1 level were measured using ELISA assay kit and ROS level using flow cytometry. Level of FN, TGF-β1 and ROS, on CKD cells model (5 mM, 10 mM glucose-induced mesangial cell) treated with EEDS 6.25 µg/mL were lower significantly compared to positive control, EEDS improve cells viability and decrease FN, TGF-β1 and ROS level in glucose-induced kidney mesangial cells as CKD cells model