Jurnal Fitopatologi Indonesia
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Effectiveness of Various PGPR Lineage in Controlling White Rust Diseases and Growth Promotion of Chrysanthemum
Full text linkKarat putih merupakan salah satu penyakit penting pada tanaman krisan di Indonesia. Teknologi yang efektif untuk pengendalian penyakit ini di Indonesia tidak tersedia. Penelitian ini bertujuan menguji potensi lima galur (Pseudomonas diminuta P14, Bacillus firmus J8, Lysinibacillus fusiformis C71, AKBR, AKS) plant growth promoting rhizobacteria (PGPR) untuk pengendalian penyakit karat putih yang disebabkan Puccinia horiana pada tanaman krisan di lapangan. Formulasi komersial Pseudomonas fluorescens dan Bacillus polymyxa dipilih sebagai pembanding. Percobaan dilakukan di lapangan dengan mengaplikasikan suspensi rizobakteri pada tanaman krisan. Semua galur PGPR yang diuji mampu menekan insidensi dan keparahan penyakit karat putih. PGPR galur Bacillus firmus J8 paling efektif menekan karat putih, sedangkan galur Lysinibacillus fusiformis C71 paling efektif meningkatkan pertumbuhan tanaman krisan. Kelima galur rizobakteri dan formulasi komersial meningkatkan diameter bunga.Effectiveness of Various PGPR Lineage in Controlling White Rust Diseases and Growth Promotion of Chrysanthemum
White rust disease caused by Puccinia horiana is the most destructive disease of Chrysanthemum in Indonesia. There was no available effective control measure against the disease in Indonesia. This research aimed to examine the potency of five strains of plant growth-promoting rhizobacteria (PGPR) for controlling white rust disease on Chrysanthemum under field conditions. Five PGPR strains (Pseudomonas diminuta P14, Bacillus firmus J8, Lysinibacillus fusiformis C71, AKBR, AKS) and one commercially formulated PGPR were used in this study. The research was carried out in farmer fields with a randomized complete block experimental design. PGPR was applied by plant watering using bacterial suspension. All PGPR strains were effective in suppressing the white rust disease incidence and its severity. The most effective PGPR strain to suppress white rust disease was Bacillus firmus J8 and the most effective strain to increase the growth of Chrysanthemum plants was Lysinibacillus fusiformis C71. The five PGPR strains tested and commercial formulation increased the diameter of the flower
Kombinasi Agens Pengendali Hayati untuk Pengendalian Penyakit Bawang Merah di Lapangan
Full text linkProduksi bawang merah di Indonesia menghadapi permasalahan hama dan penyakit yang berat. Penggunaan pestisida menjadi andalan petani hingga saat ini untuk pengendalian hama dan penyakit tersebut. Beberapa agens biokontrol hama dan patogen bawang merah terbukti efektif secara individual, namun belum terintegrasi di lapangan. Tujuan penelitian ialah mengevaluasi kombinasi agens biokontrol dengan efektivitas terbaik untuk menekan insidensi penyakit utama bawang merah di lapangan. Percobaan lapangan dilakukan di Tegal, Jawa Tengah, salah satu sentra penghasil bawang merah di Indonesia. Perlakuan yang diuji ialah kombinasi Fusarium nonpatogenik (FNP) + plant growth promoting rhizobacteria (PGPR)+ khamir antagonis (KA), FNP + KA, PGPR + KA, FNP+PGPR, fungisida sintetik, dan tanpa perlakuan (kontrol). Penelitian disusun dalam rancangan acak kelompok dengan empat ulangan sebagai blok. Semua perlakuan kombinasi agens pengendali hayati dan fungisida sintetik menunjukkan perbedaan nyata dengan perlakuan kontrol dalam menekan penyakit busuk batang (Fusarium oxysporum f. sp. cepae) dan bercak ungu (Alternaria porri), tetapi tidak berbeda nyata antarperlakuan. Produktivitas bawang merah dengan perlakuan agens pengendali hayati memiliki bobot umbi segar yang cukup tinggi. Tanaman dengan perlakuan agens hayati PGPR + KA dan FNP + PGPR memiliki bobot umbi segar yang paling tinggi di antara perlakuan agens pengendali hayati lainnya. Penelitian ini menunjukkan bahwa aplikasi kombinasi agens pengendali hayati berpotensi menekan penyakit bawang merah di lapangan.Combination of Biocontrol Agents to Control Shallot Disease in The Field
Shallot production in Indonesia faces serious pest and disease problems. The use of pesticides has been using as the main practice by shallot farmers for controlling pests and diseases. Several biocontrol agents for shallot pests and pathogens have been shown to be effective individually, but have not been integrated in the field. The aim of the study was to evaluate the combination of biocontrol agents with the best effectiveness in reducing the incidence of the main shallot diseases in the field. Field trials were conducted in Tegal, Central Java, one of the shallots producing regions in Indonesia. The treatment consisted of a combination of non-pathogenic Fusarium (FNP) + plant growth promoting rhizobacteria (PGPR) + yeast antagonist (KA), FNP + KA, PGPR + KA, FNP + PGPR, synthetic fungicide, and no treatment (control). The study was arranged in a randomized block design with four block replications. All combination of biological control agents and synthetic fungicides showed significant differences with the control treatment in suppressing stem rot (Fusarium oxysporum f. sp. cepae) and purple spot (Alternaria porri) diseases, but not significantly different among them. The productivity of shallots treated with biological control agents had a relatively high fresh tuber weight. Plants treated with PGPR + KA and FNP + PGPR had the highest fresh tuber weight among other biological control agents. This study shows that the application of a combination of biological control agents has the potential to suppress shallot disease in the field
Resistance of Six Lines from Farmers against Bacterial Leaf Blight (Xanthomonas oryzae pv. oryzae)
Full text linkPenyakit hawar daun bakteri (HDB) yang disebabkan oleh Xanthomonas oryzae pv. oryzae merupakan salah satu penyakit yang merugikan bagi produksi padi Indonesia. Salah satu upaya untuk mengendalikan penyakit tersebut ialah menggunakan varietas tahan HDB. Penelitian ini bertujuan untuk mengevaluasi perkembangan penyakit HDB pada enam galur hasil rakitan dari petani (TCIPB202101, TCIPB202102, TCIPB202103, TCIPB202104, TCIPB202105, dan TCIPB202106) dan dua varietas pembanding, yaitu Ciherang dan Inpari 30. Penelitian dilakukan di rumah kaca Cikabayan Dramaga dengan inokulasi buatan. Pengamatan meliputi masa inkubasi, panjang lesio dan intensitas penyakit pada 7 dan 14 hari setelah inokulasi (HSI). Hasil penelitian menunjukkan semua galur yang diuji merupakan galur yang tahan terhadap penyakit HDB dan memiliki ketahanan yang lebih baik dibandingkan dengan varietas pembandingnya. Galur TCIPB202105 dan TCIPB202106 memiliki respons ketahanan terbaik dengan rata-rata intensitas penyakit dibawah 1%, sedangkan TCIPB202101 dan TCIPB202102 memiliki intensitas penyakitnya yang paling tinggi namun masih dalam kategori agak tahan.Resistance of Six Lines from Farmers against Bacterial Leaf Blight (Xanthomonas oryzae pv. oryzae)
Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is a disease that detrimental to rice production in Indonesia. One effort to control the disease is to use BLB resistant varieties. This study aims to evaluate the development of bacterial leaf blight on six lines (TCIPB202101, TCIPB202102, TCIPB202103, TCIPB202104, TCIPB202105, and TCIPB202106) and two control varieties (Ciherang and Inpari 30). Green house experiment was conducted in Cikabayan Dramaga using artificial inoculation. The study was conducted by observing the incubation period, lesion length and disease intensity at 7 and 14 days after inoculation (DAI). The results showed that all the lines tested were resistant to BLB and had better resistance compared to the control varieties. The TCIPB202105 and TCIPB202106 lines had the best resistance response with an average disease intensity below 1%. Meanwhile, the TCIPB202101 and TCIPB202102 lines had the highest percentage of disease intensity although they are still considered as moderately resistant category
Cover Jurnal Fitopatologi Vol. 18 No. 5, September 2022
No full textThis editorial contains the front cover, editorial page, and back cover of the Jurnal Fitopatologi Indonesia Vol. 18 No. 5, September 2022This editorial contains the front cover, editorial page, and back cover of the Jurnal Fitopatologi Indonesia Vol. 18 No. 5, September 202
Keanekaragaman Karakter Galur-Galur Bakteri Penyebab Busuk Hitam (Xanthomonas campestris pv. campestris) pada Kubis terhadap Campuran Bahan Aktif Azoksistrobin dan Difenokonazol
Full text linkCharacter Diversity of Black Rot Bacterial Strains (Xanthomonas campestris pv. campestris) on Cabbage against Mixture of Active Ingredients Azoxystrobin and Diphenoconazole
Xanthomonas campestris pv. campestris is a bacterium that causes black rot on cabbage. Farmers still rely on the synthetic pesticides azoxystrobin and difenoconazole to control diseases in cabbage which are feared to have an impact on the diversity of X. campestris pv. campestris. The objective of the research was to obtain genetic and phenotypic diversity data on X. campestris pv. campestris, as well as obtaining data on the resistance response of these bacterial isolates to a mixture of the active ingredients azoxystrobin and difenoconazole. The study consisted of five stages (1) sampling on land with black rot symptoms; (2) isolation of bacteria using tissue implant technique; (3) selection of isolates by testing properties of Gram, hypersensitivity, starch hydrolysis, and pathogenicity; (4) identification using specific and universal 16S rRNA primers; and (5) analysis of genotypic diversity by in silico RFLP method and phenotypic diversity by measuring EPS weight. The results of molecular characterization and identification obtained five isolates of X. campestris pv. campestris (CLT01, CDA08, SDA02, SDA22, and SDA26). The results of the analysis of genotypic diversity showed that the five isolates had genetic diversity based on the cutting of the 16S rRNA gene DNA fragment, while phenotypically indicated different Inhibition concentration (IC) values. SDA22 isolate had the highest IC50 value and different genetic diversity compared to other X. campestris pv. campestris. The use of synthetic pesticides azoxystrobin and difenoconazole continuously for a long time is feared to have an impact on pathogenic microbes such as X. campestris pv. campestris. So that other control alternatives are needed so that there is no resistance to these pathogenic microbes.Xanthomonas campestris pv. campestris merupakan bakteri penyebab penyakit busuk hitam (black rot) pada kubis. Petani masih mengandalkan pestitida sintetis azoksistrobin dan difenokonazol untuk mengendalikan penyakit pada tanaman kubis yang dikhawatirkan akan berdampak pada keanekaragaman X. campestris pv. campestris. Tujuan penelitian ialah mendapatkan data keanekaragaman secara genetik dan fenotipik isolat bakteri X. campestris pv. campestris, serta mendapatkan data respons resistensi isolat bakteri tersebut terhadap campuran bahan aktif azoksistrobin dan difenokonazol. Penelitian terdiri atas lima tahapan, yaitu (1) pengambilan sampel pada lahan yang terdapat gejala busuk hitam; (2) isolasi bakteri menggunakan teknik penanaman jaringan; (3) seleksi isolat dengan uji sifat Gram, hipersensitif, hidrolisis pati, dan patogenesitas; (4) identifikasi menggunakan primer spesifik dan universal 16S rRNA; dan (5) analisis keanekaragaman genotipe dengan metode RFLP in silico dan keragaman fenotipe dengan mengukur bobot eksopolisakarida. Hasil karakterisasi dan identifikasi secara molekuler diperoleh lima isolat bakteri X. campestris pv. campestris, yaitu CLT01, CDA08, SDA02, SDA22, dan SDA26. Hasil analisis keanekaragaman genotipe menunjukkan bahwa kelima isolat memiliki keanekaragaman berdasarkan pemotongan fragmen DNA gen 16S rRNA, sedangkan keragaman fenotipe ditunjukkan dengan nilai inhibition consentration (IC) yang berbeda-beda. Isolat SDA22 memiliki nilai IC50 paling tinggi dan keanekaragaman secara genetik yang berbeda dibandingkan dengan isolat X. campestris pv. campestris lainnya. Penggunaan pestitida sintetis azoksistrobin dan difenokonazol secara terus-menerus dalam jangka waktu lama dikhawatirkan berdampak pada patogen seperti X. campestris pv. campestris. Oleh karena itu, alternatif pengendalian yang lain diperlukan agar tidak terjadi resistensi pada X. campestris pv. campestris
Development of Specific Detection for Mungbean Yellow Mosaic India Virus Infecting Yard Long Bean in Java, Indonesia
Full text linkPengembangan Deteksi Spesifik untuk Mungbean Yellow Mosaic India Virus yang Menginfeksi Kacang Panjang di Jawa, Indonesia
Penyakit mosaik kuning pertama kali dilaporkam di Indonesia pada tahun 2018. Infeksi penyakit ini pada tanaman kacang panjang mengakibatkan kehilangan hasil yang nyata. Mungbean yellow mosaic India virus (MYMIV), anggota dari genus Begomovirus, diidentifikasi sebagai penyebab utama penyakit mosaik kuning tersebut. Deteksi yang spesifik dan akurat sangat diperlukan untuk pemantauan penyakit sebagai bagian dari strategi pengendalian penyakit. Penelitian ini bertujuan merancang primer spesifik untuk deteksi cepat MYMIV menggunakan metode polymerase chain reaction. Primer MY1/MY2 dirancang untuk mengamplifikasi sebagian protein selubung dari MYMIV. Pengujian secara in silico dan in vitro menunjukkan bahwa primer spesifik MY1/MY2 mampu mengamplifikasi secara spesifik MYMIV.Yellow mosaic disease was reported for the first time in Indonesia in 2008. Its infection on yard long bean caused significant yield loss. Mungbean yellow mosaic India virus (MYMIV), member of genus Begomovirus was identified as the main causal agent. Specific and accurate detection is important for disease monitoring as part of disease management strategy. The aim of this study was to construct specific primer pairs for quick and robust detection of MYMIV using polymerase chain reaction method. A pair of primers MY1/MY2 was designed in this study to amplify part of MYMIV coat protein. In silico and in vitro test showed that MY1/MY2 primers specifically amplified MYMIV
Intensity of Main Disease in Several Superior Sugarcane Clones at Krebet Baru Sugar Factory, Malang
Full text linkTebu (Saccharum officinarum) merupakan tanaman perkebunan penting sebagai penghasil utama gula di Indonesia. Penurunan produksi gula tebu dalam beberapa tahun terakhir tidak sejalan dengan permintaan gula tebu yang kian meningkat. Pemuliaan tanaman diarahkan untuk mendapatkan varietas-varietas tebu unggul yang diharapkan memiliki produktivitas tinggi dan tahan terhadap faktor-faktor gangguan yang dapat menurunkan produktivitas tanaman. Pengamatan penyakit mosaik bergaris (Sugarcane streak mosaic virus/SCSMV), pokahbung (Fusarium moniliforme), dan luka api (Sporisorium scitamineum) dilakukan pada 14 klon tebu unggul di PG Krebet Baru, Malang. Pengamatan intensitas penyakit dilakukan setiap 2 minggu selama 3 bulan dan sampel tanaman yang menunjukkan gejala penyakit dideteksi penyebab penyakitnya di laboratorium. Gejala penyakit mosaik bergaris terjadi pada tiga klon tebu dengan intensitas antara 8.33% dan 63.89%; sedangkan gejala penyakit pokahbung dan luka api ditemukan pada 11 dan 14 klon tebu dengan intensitas berturut-turut 2.78% sampai 22.22% dan 11.11% sampai 25%. Konfirmasi keberadaan SCSMV diperiksa dengan metode polymerase chain reaction; sedangkan cendawan F. moniliforme dan S. scitamineum melalui isolasi jaringan dan pengamatan jaringan meristem. Secara umum insidensi penyakit tergolong rendah pada klon 8 dan 12 sehingga klon tersebut dapat direkomendasikan sebagai klon potensial yang digunakan dalam mengendalikan penyakit utama tebu.Intensity of Main Disease in Several Superior Sugarcane Clones at Krebet Baru Sugar Factory, MalangSugarcane (Saccharum officinarum) is an important plantation crop in Indonesia as the major source of sugar production. Cane sugar production decreased significantly in recent years and it is not in line with its demand which is increasing from year to year. Many studies have been carried out to obtain sugarcane varieties which are expected to have high production and are resistant or tolerant to factors constraining crop production. Observations of the main sugarcane diseases, i.e. sugarcane streak mosaic (Sugarcane streak mosaic virus/SCSMV), pokkah boeng (Fusarium moniliformae), and sugarcane smut (Sporisorium scitaminae) were carried out on 14 potential sugarcane clones in PG Krebet Baru, Malang. Observations of disease intensity were carried out every 2 weeks for 3 months and plant samples showing disease symptoms were brought to the laboratory to detect the cause of the disease. Specific symptoms of sugarcane streak mosaic, pokkah boeng and sugarcane smut diseases were observed in the field involving mosaic, chlorosis on young leaves and smut, respectively. Mosaic symptom was found only on 3 clones with disease intensity ranging from 8.33% to 68.89%; whereas chlorosis and smut symptom was found on 11 and 14 clones with disease intensity ranging from 2.78% to 22.22% and 11.11% to 25%, respectively. Laboratory examination using the polymerase chain reaction method confirmed the presence of SCSMV; while the fungi F. moniliforme and S. scitamineum were confirmed through tissue isolation and observation of meristem tissue, respectively. In general, disease incidence was relatively low in clones 8 and 12 and these clones can be recommended as potential clones used in controlling the main diseases of sugarcane.
 
Penurunan Risiko Perkembangan Cendawan Terbawa Benih Hotong asal Pulau Buru melalui Perlakuan Air Panas
No full textPenurunan Risiko Perkembangan Cendawan Terbawa Benih Hotong (Setaria italica) Asal Pulau Buru Melalui Perlakuan Air Panas
Penggunaan benih sehat yang bebas cendawan terbawa benih merupakan faktor penting untuk meningkatkan produksi hotong (Setaria italica). Penelitian ini bertujuan untuk memperkaya teknik eliminasi cendawan terbawa benih melalui perlakuan air panas terhadap benih hotong asal Pulau Buru. Identifikasi cendawan terbawa benih menunjukkan bahwa Boeremia merupakan cendawan dominan pada benih hotong asal Pulau Buru. Perlakuan air panas dengan kombinasi suhu (50 ℃, 52 ℃, 54 ℃) dan lama perlakuan (15, 20, 25 menit) diujikan untuk mencari perlakuan yang paling efektif menekan pertumbuhan cendawan tetapi tidak mengganggu perkecambahan benih. Penelitian ini menunjukkan bahwa perlakuan air panas pada suhu 50 ℃ selama 25 menit mampu menurunkan infestasi cendawan pada perlakuan in vitro dan ad planta berturut-turut sebesar 57% dan 55%. Hal ini mengindikasikan bahwa metode ini dapat diandalkan untuk menyelamatkan kerugian produksi benih hotong akibat perkembangan cendawan patogen.The use of fungi-free seeds is an important factor to increase the production of foxtail millet (Setaria italica). This study aims to develop a technique to eliminate seed-borne fungi of foxtail millet seeds from Buru Island through hot water treatment. Identification of seed-borne fungi showed that Boeremia sp. was the dominant fungus on foxtail millet seeds from Buru Island. Hot water treatment with a combination of temperature (50 ℃, 52 ℃, 54 ℃) and treatment period (15, 20, 25 minutes) was examined to determine the most effective treatment in suppressing the growth of fungi but not affecting seed germination. This study showed that hot water treatment at 50 ℃ for 25 minutes reduced fungal infestation in vitro and ad planta by 57% and 55%, respectively. This indicates that this method can be relied upon to save foxtail millet seed production losses due to the development of pathogenic fungi
Nematoda Parasit Tumbuhan pada Tanaman Stroberi di Ciwidey-Jawa Barat
Full text linkStroberi merupakan tanaman hortikultura yang banyak dibudidayakan di dataran tinggi. Salah satu hambatan dalam produksi stroberi ialah infeksi nematoda parasit tanaman. Kecamatan Ciwidey merupakan salah satu sentra produksi stroberi di Kabupaten Bandung, Provinsi Jawa Barat. Beberapa kebun stroberi di Ciwidey menunjukkan gejala serangan nematoda yang memakan bagian akar dan tajuk tanaman. Laporan nematoda parasit tanaman pada tanaman stroberi di Indonesia masih terbatas sehingga penelitian ini bertujuan mengidentifikasi genus nematoda yang berasosiasi dengan stroberi dan menghitung kelimpahannya. Sampel tanaman diambil dengan metode pemilihan dengan sengaja pada tanaman yang bergejala. Sampel tanah diekstraksi dengan metode flotasi sentrifugasi, sampel akar dengan metode pengabutan; sedangkan sampel daun dipotong-potong, direndam di dalam cawan, lalu diinkubasi di lemari pendingin selama 24 jam. Nematoda diidentifikasi dan dihitung kelimpahannya. Gejala serangan nematoda parasit tanaman yang ditemukan pada tajuk tanaman stroberi berupa pertumbuhan terhambat, daun memerah, daun kecil menggulung atau berkerut, dan klorosis. Gejala pada akar ialah berupa lesio akar, akar memendek, jumlah akar berkurang, pembengkakan pada ujung akar, dan puru akar. Nematoda dari seluruh sampel ialah Aphelenchoides besseyi, A. bicaudatus, Meloidogyne spp., Pratylenchus sp., Scutellonema sp., dan Tylenchus sp. Kelimpahan nematoda yang didapatkan bervariasi antara 1–42 ekor nematoda 100 mL-1 tanah dan 2–29 ekor 5 g akar-1 . Diantara nematoda yang ditemukan, Scutellonema merupakan nematoda yang pertama kali dilaporkan berasosiasi dengan tanaman stroberi di Indonesia.Plant Parasitic Nematode of Strawberry in Ciwidey-West Java
Strawberry is a horticultural crop that is widely cultivated in the highlands. One of the obstacles in strawberry production is plant parasitic nematode infection. Ciwidey District is one of the strawberry production centers in Bandung Regency, West Java Province. Several strawberry farms in Ciwidey showed symptoms of nematode infection, which affect the roots and crowns of the plants. There is limited reports of plant parasitic nematodes on strawberry plants in Indonesia , therefore this study was initiated to identify the genera of nematodes associated with strawberries and measure their abundance. Plant samples were taken by a purposive sampling method on symptomatic plants. Soil samples were extracted by centrifugation floatation method, root samples by fogging method; while leaf samples were cut into pieces, soaked in a cup, then incubated in the refrigerator for 24 hours. Nematodes were identified and their abundance was counted. Symptoms of plant parasitic nematode on the strawberry plant canopy involved stunted growth, reddened leaves, curled or wrinkled small leaves, and chlorosis. Symptoms on the roots are in the form of root lesions, shortened roots, reduced number of roots, swelling at the tip of the root, and root galls. Six species of nematodes were identified from all samples, i.e. Aphelenchoides besseyi, A. bicaudatus, Meloidogyne spp., Pratylenchus sp., Scutellonema sp., and Tylenchus sp. The abundance of nematodes found varied between 1–42 nematodes per 100 mL-1 soil and 2–29 nematodes per 5 g root-1. Among the nematodes found, Scutellonema was the first nematode reported to be associated with strawberry plants in Indonesia
Isolation and Morphological Characterisation of Cercospora janseana Infecting Rice Leaves
Full text linkPenyakit bercak cokelat sempit pada tanaman padi menjadi salah satu penyakit yang paling merugikan. Di Indonesia, informasi mengenai penyakit ini masih terbatas dan kurang mendapatkan perhatian petani dan peneliti. Pengetahuan dasar mengenai patogen penyebabnya—Cercospora janseana—diperlukan sebagai acuan dalam tindakan pengendalian dan memahami epidemiologinya di masa mendatang. Penelitian ini bertujuan menentukan teknik isolasi yang sesuai untuk C. janseana serta melakukan karakterisasi morfologinya dari beberapa varietas tanaman padi. Teknik isolasi yang digunakan ialah metode penanaman jaringan, suspensi spora, penyebaran spora, dan penempelan spora. Pengamatan morfologi dilakukan dengan mengamati warna dan pertumbuhan koloni, konidium dan konidiofor, serta pertumbuhan koloni pada medium tumbuh yang berbeda. Hasil penelitian menunjukkan bahwa teknik isolasi yang sesuai untuk C. janseana ialah dengan penempelan spora pada agar-agar air dan menumbuhkannya pada medium agar-agar Martin. Teknik ini lebih baik dibandingkan dengan tiga teknik isolasi lainnya dan berhasil memudahkan proses isolasi C. janseana hingga diperoleh isolat murni.Isolation and Morphological Characterisation of Cercospora janseana Infecting Rice Leaves
Narrow brown spot disease in rice plants is among the most destructive diseases. In Indonesia, information about this disease still needs to be improved and needs to get the attention of farmers and researchers. Basic knowledge of the causative pathogen— Cercospora janseana—is needed as a reference in controlling measures and understanding its epidemiology in the future. This study aimed to determine the appropriate isolation techniques for C. janseana and to characterize the morphology of four varieties of rice plants. The isolation techniques used were the method of tissue planting, spore suspension, spore dispersal, and spore attachment. Morphological characterization was carried out by observing the colors and growth of colonies, conidia, and conidiophore, as well as the growth of colonies on different culture media. The results showed that the suitable isolation technique for C. janseana was attaching the spores to water agar and growing them on Martin agar medium. This technique was better than the other three isolation techniques and facilitated the isolation process of C. janseana to obtain pure isolates