Biology, Medicine, & Natural Product Chemistry
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Evaluation and Antibacterial Activity Test of Peel-off Mask Preparation from Combination of Pegagan Leaves (Centella asiatica (L) Urb.) and Charcoal Powder
Get PDFPegagan leaf (Centella asiatica (L) Urb.) is a plant that contains flavonoids, tannins, triterpenoids, and saponins that function as antibacterials. Pegagan leaf is combined with activated charcoal powder, which has the job of absorbing facial oil, so that it is formulated in the form of a peel off mask to produce a synergistic effect. This study aims to formulate, evaluate, and test the antibacterial effectiveness of the peel-off mask preparation of pegagan leaf extract at concentrations of 1.5%, 2%, and 2.5%. This study uses an experimental method by testing the parameters of the physical evaluation requirements and testing antibacterial activity with the disc method on the peel off mask preparation. The results were evaluated for 28 days, and the results of the organoleptic test were stable, homogeneity stable, pH (5.6–6.2), dry time (16–25 minutes), dispersion (6.1-6.4 cm), and irritation (2 respondents). The effectiveness test against the bacteria Propionibacterium acnes has inhibitory power of 12.3 mm in the strong category. So, it can be concluded that the peel off mask produced has stable physical characteristics and can be used as an antibacterial mask
The Recent Collection of Climacodon roseomaculatus (Hydnum roseomaculatum Henn. & E. Nyman) from The Type Locality (Indonesia)
Get PDFClimacodon roseomaculatus is a fleshy species of Polyporales with a hydnoid hymenophore. The species’s distribution in Indonesia was reported six times from 1923 to 1961 from West Java and East Java collection to date, there have been no following updates on the collection and occurrence status of C. roseomaculatus in Indonesia. During a fungus foray in 2023, some red to pink basidiomata of Climacodon were spotted in Semarang, Central Java, Indonesia. The current study aimed to determine the taxonomical position of Climacodon BO24632 based on morphological evidence. Fresh materials were used for the investigation of macro- and micromorphological characters. The result showed that Climacodon BO24632 was identified as C. roseomaculatus based on distinctive features of stipe and gleocystidia. Climacodon roseomaculatus BO24632 is closely related to C. sanguineus with blood-red to pink basidiomata, cylindrical spines of hymenophores, and ellipsoid basidiospores. However, the former species lacks a well-developed stipe and has thin to thick-walled gloeocystidia. This study is the first report on the distribution of C. roseomaculatus from Central Java, Indonesia. In addition, the current work is the latest information and herbarium collection of C. roseomaculatus after more than fifty years of hiatus in Indonesia. We expect that the same species also occur in many different locations in Indonesia; thus need a warrant of collections
Genotoxic and Cytotoxic Activities of Cornhusk Extract of Zea mays and Leaf Extract of Sacharum officinarum
Get PDFZea mays husk and Saccharum officinarum have been used for years in ethnomedicine for their antimalarial, anti-inflammatory, antipyretic, antidiabetic, and antiphlogistic activities. The cytotoxic and genotoxic effects of Zea mays husk and Saccharum officinarum leaf extracts on the root meristem cells of Allium cepa were investigated. Onion bulbs were exposed to 2.5 mg/ml, 5mg/ml, and 10 mg/ml concentrations of the extracts for macroscopic and microscopic analysis. Tap water was used as a negative control and Methotrexate (0.1 mg/ml) was used as a positive control. There was statistically significant (p < 0.05) inhibition of root growth depending on concentration by the extracts when compared with the negative control group. All the tested extracts were observed to have cytotoxic effects on cell division in A. cepa. The extract induced chromosomal aberrations and micronuclei (MNC) formations in A. cepa root tip cells were significant (p<0.05) when compared with the control group. The extracts treatment further induced cell death, ghost cells, cells membrane damage, and binucleated cells. The Zea mays husk extract was found to exhibit higher cytotoxic and genotoxic potential than Saccharum officinarum leaf extract. These results suggest that Zea mays husk and Saccharum officinarum leaf extracts possess cytotoxic and genotoxic effects on A. cepa
Potential of California Variety Papaya Leaf Extract (Carica papaya L. California variety) As Bioherbicide of Snake Grass Weed (Cyperus rotundus L.)
Get PDFEfforts to increase production yields are by controlling weeds. Weeds that often grow and we encounter in cultivated plants are weed grass (C. rotundus). California papaya leaves have the potential to be a bioherbicide because they contain allelochemicals that can inhibit weed growth. This study aims to determine the effect of papaya leaves (C. papaya) on the growth of teki grass (C. rotundus) and determinetheconcentration level of papaya leaf allelopathy that most effectively inhibits the growth of teki grass.The method used was a completely randomized design (CRD) with five replications and four treatmentsof0%, 30%, 50%, and 70%. For data analysis in this study using the SPSS version 25.0 program. Papaya leaf extract (C. papaya) is able to inhibit the growth of weed grass (C. rotundus). The concentration of papaya leaf extract (C. papaya) that most effectively inhibits the growth of weed is 50% concentration with a mortality rate (64%), for the lowest plant heightis50% concentration which is 13.84 cm, for the slowestgrowthrate is 50% concentrationwith a growthrate of -0.16 cm
Phytochemical Profile and Antibacterial Activity of Nigella Sativa against Biofilm-producing Bacteria Uropathogens
Get PDFThis study explores the antibacterial effects of Nigella sativa seeds on bacteria obtained from clinical samples. The aim was to assess the antibacterial properties of both aqueous and methanolic extracts of Nigella sativa seeds against E. coli, S. aureus, and P. aeruginosa. The three samples were collected from the Microbiology Laboratory of Modibbo Adamawa Medical Centre and were reconfirmed using culture, microscopy, and some biochemical tests. The seed samples of N. sativa were procured from herbal point Yola, Adamawa State, Nigeria. The phytochemical assay of the extracts revealed the presence of flavonoids, alkaloids, tannins, phenols, cardiac glycosides, steroids, saponins, and terpenoids in both extracts. The highest antibacterial activity against S. aureus, E. coli, and P. aeruginosa was demonstrated by the aqueous extract of N. sativa seeds, with inhibition zone diameters of 19.30 ±0.61 mm, 8.10 ±2.17 mm, and 12.00 ±0.29 mm, respectively. However, the methanol extract exhibited slightly greater activity against E. coli and P. aeruginosa, with inhibition zone diameters of 12.10 ±0.38 mm and 13.80 ±0.40 mm, respectively. Both methanol and aqueous extracts showed minimum inhibitory concentrations (MICs) of 25 mg/mL against S. aureus and E. coli. Similarly, for P. aeruginosa, the MIC was 25 mg/mL for methanol extract and 50 mg/mL for aqueous extract. The minimum bactericidal concentration (MBC) for both extracts against S. aureus and E. coli was determined to be 25 mg/mL. However, for P. aeruginosa, the MBC was 25 mg/mL for the aqueous extract and 50 mg/mL for the methanol extract. The study indicates that N. sativa seed extract possesses antibacterial properties against S. aureus and P. aeruginosa, underscoring its potential as an effective medicinal antibacterial agent
Antibacterial Activity of Sweet Orange (Citrus sinensis) Peel Tea against Enterobacteriaceae Isolated from a Water Depot
Get PDFMost orange peels are not utilized and become useless waste that is thrown away. Orange peels contain many nutrients and compounds, such as flavonoids, terpenoids, alkaloids, and essential oils, which are secondary metabolites that act as antibacterial agents. This study intends to investigate the antibacterial activity of sweet orange peel tea against isolated Enterobacteriaceae from a random water depot sample around Universitas Prima Indonesia. This experiment used disc diffusion for antibacterial assay. Meanwhile, the sweet orange peel was brewed using two different methods, including infusion and decoction, in two different masses (3 grams and 5 grams). These sweet orange peel tea formulations were compared to standard (chloramphenicol) and control (distilled water). Enterobacteriaceae was isolated and identified from a random water depot sample around Universitas Prima Indonesia, which included colony identification in EMB agar and MacConkey agar, gram staining, and biochemical test. This study showed that a random water depot sample contaminated by Enterobacteriaceae had properties similar to Citrobacter sp. Sweet orange tea formulation inhibited this isolated Enterobacteriaceae growth (P-Value: 0.010). Antibacterial activity was observed in 3-gram infusion, 5-gram infusion, and 5-gram decoction. However, the antibacterial activity was not better than the standard (chloramphenicol). Overall, it can be concluded that the sweet orange peel tea as infusion or decoction has weak antibacterial activity against Enterobacteriaceae bacteria isolated from water depots, which had some properties similar to Citrobacter sp
The Diversity of Understory (Shrubs and Herbs) in the Kalikuning Area
Get PDFKalikuning is one of the areas that has a diversity of flora. Kalikuning's fertile land helps a variety of plants flourish there. Since there are no people living here, a wide variety of unidentified and underexplored plants can be known. Therefore, more research on the plant diversity is required. The research aims to invent, document, and find out the diversity of the understory (shrubs and herbs) in Kalikuning. This research used survey and exploration methods. The researchers documented shrub and herb species. Based on the results, 28 families consist of 65 species of shrubs and herbs. Those species are flora that have specific characteristics
Antimicrobial Activities of Oyster Mushroom (Plerotous ostreatus), Garlic (Allium sativum), and Ginger Extracts (Zingiber officinale) Against Some Clinical Isolates
Get PDFThe prevalence of multiple drug resistance among human pathogenic bacteria necessitates a continual search for new antimicrobial medicines, particularly among plants that are frequently farmed or naturally found in our surroundings. The study aims to investigate the inhibitory effect of garlic, ginger, and mushroom extracts at different concentrations against some clinical isolates. Anti-bacterial components from mushrooms, garlic, and ginger were extracted with hot water, cold water, ethanol, and acetone, and their anti-bacterial activity was determined using the agar well diffusion method. Zones of inhibition were observed primarily on hot water extracts of mushrooms (Pleurotus ostreatus) on culture plates inoculated with Staphylococcus aureus, Escherichia coli, and Klebsiella pneumonia at 370C for 24 hours. The cold water extracts of the mushroom (Pleurotus ostreatus) gave the highest zone of inhibition of 14.0±1.0mm when used against S. aureus. For spices, the cold water extracts yielded the highest zones of inhibition of 22.0±1.0mm followed by 16.0±1.0mm as observed with ginger. The results obtained have shown clearly that the mushrooms (Pleurotus ostreatus), garlic (Allium sativum), and ginger (Zingiber officinale) extracts contain phytochemicals with some antimicrobial activities. The water extracts of the mushrooms and spices showed broad-spectrum antimicrobial activity much more than ethanol and acetone extracts. The antimicrobial activities of mushroom and garlic extracts were highly effective against the bacterial pathogens studied. However, the antimicrobial activity of the ginger extract was poor. To address the multi-drug resistance to antibiotics, I recommend: that bioactive compounds found in mushrooms, ginger, and garlic be patented and used as alternative antimicrobials
Formulation and Antibacterial Activity Testing of Eco-Soap Based on Sodium Lauryl Sulfate Surfactant
Get PDFPineapple is a fruit that is often used only for its flesh, while the skin remains waste. As a form of dealing with pineapple waste, one way is to use it as an eco-enzyme. Making soap with added eco-enzyme can be used as an alternative for washing dishes. Therefore, this research aims to determine the formulation and test the antibacterial activity of eco-soap based on the surfactant Sodium Lauryl Sulphate. This research is experimental research, which was carried out in July-October 2023. The research results showed that the pH of eco-Soap was 3.46; foam height 1.5 cm; viscosity testing 14.9 mPas; fatty acids 1.44%; and 6 mm antibacterial activity testing. Based on the research results, it was concluded that the surfactant-based eco-soap formulation had good stability in the eco-soap foam height test. The diameter of the zone of inhibition of eco-soap's antibacterial activity showed that bacterial inhibition was moderate
In vivo Alpha-amylase and Alpha-glucosidase Inhibitory Potentials of Panicum maximum Jacq. (Guinea grass) Leaf Extract on Wister Rats
Get PDFPanicum maximum Jacq. (Guinea grass) a medicinal plant used traditionally in the treatment of diseases including diabetes was evaluated for its effect on alpha amylase and alpha glucosidase enzymes in vivo. The crude ethanol extracts (150, 300 and 450 mg/kg) of P. maximum were investigated using starch, sucrose, glucose and maltose as substrates and acarbose as reference drug. The leaf extract caused significant (p<0.05) reduction in blood glucose levels of the treated rats with the four substrates used. The findings show that the leaf extract of Panicum maximum has the potentials to inhibit alpha amylase and alpha glucosidase in rats