Jurnal Teknologi Laboratorium
Not a member yet
97 research outputs found
Sort by
Does platelet/ lymphocyte ratio a predictor of CD34+ peripheral blood stem cell yield in the healthy donors mobilized with GCSF?
No full textThis research aimed to determine whether there is a correlation between platelet/lymphocyte ratio the number of collected CD34+ stem cells. This retrospective study included 94 adult related stem cell donors who were healthy and volunteer by screening their files between the years 2016 and 2018. All donors were mobilized using 2.5 mcg/kg lenograstim or filgrastim and underwent peripheral stem cell apheresis. Complete blood counts were tested at baseline before G-CSF administration (pre–G-CSF), and before PBSC collection after mobilization with G-CSF administration. The patients were divided into two groups as aged below and over 50 years old. From these comparative data, only BMI value of the group aged below 50 years was statistically significantly lower than the other group, whereas no statistically significant difference was found between the groups in terms of other parameters. The numbers of the collected CD34+ stem cells of both age groups were similar, and no significant difference was found. The values of platelet/lymphocyte ratio, early measurement of CD34+ stem cells and the amount of the collected CD34+ stem cells of both groups at the first and second days of the procedure were found similar. This research show that a high platelet count and consequently, a high platelet/lymphocyte ratio may be correlated with the number of collected CD34+ stem cells but our hypotheses revealed insignificant outcomes
Immunobioinformatics analysis and phylogenetic tree construction of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Indonesia: spike glycoprotein gene
Get PDFThe outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), has spread worldwide and as a result, the World Health Organization (WHO) declared it a pandemic. At present, there are no approved vaccines against SARS-CoV-2. Therefore, the aim of this study was to predict epitope-based vaccines using bioinformatics approaches and phylogenetic tree construction of SARS-CoV-2 against the backdrop of the COVID-19 pandemic. In this study, we employed 27 isolates of SARS-CoV-2 spike glycoprotein genes retrieved from GenBank® (National Center for Biotechnology Information, USA) and the GISAID EpiCoV™ Database (Germany). We analyzed the candidate epitopes using the Immune Epitope Database and Analysis Resource. Furthermore, we performed a protective antigen prediction with VaxiJen 2.0. Data for B-cell epitope prediction, protective antigen prediction, and the underlying phylogenetic tree of SARS-CoV-2 were obtained in this research. Therefore, these data could be used to design an epitope-based vaccine against SARS-CoV-2. However, the advanced study is recommended for confirmation (in vitro and in vivo)
The Physiochemical properties, sensory evaluation and shelf life of corn flour supplemented with Acheta gossypii (cricket) flour
No full textMost consumed diets are made from corn and are deficient in proteins. Corn flour was made from fermented and dried yellow corn and supplemented with 5 and 10 % proteins of crickets. The diets were analyzed for the following physiochemical properties: swelling index, wettability, bulk density, solubility, and water holding capacity as well as sensory characteristics in a 7 point hedonic scale and the maintaining quality of each diet. Standard methods for physiochemical properties were used, and the microbial assay was also to analyze the diets at refrigerated and non-refrigerated conditions. The swelling index(1.83-2.00 g), gelatinization temperature ( 66.3 -72.3°C), wettability (22.0-120 seconds), and bulk density (0.59 - 0.67 mg/mL), significantly increased but solubility (14.1 - 3.00%), and oil-water holding capacity ( 90-65 %) reduced significantly in the cricket supplemented diets over the corn flour ( p< 0.05). The sensory characteristics from both the adult and children scored above average on the 7 points hedonic scale, with a major preference for the 5 % cricket supplemented the diet. It is noteworthy that in the 4 weeks of shelf life experiment, all the diets were found to be devoid of the growth of coliforms, yeast and the only occurrence of the mold is below the harmful threshold except with the 10 % cricket supplemented non-refrigerated diet with the growth of B. subtilis on the 3rd week of the experiment. However, the growth of this bacterium is below a harmful threshold. Proper handling and processing are safest before consumption. Supplementations of corn flour with cricket have been found to improve the nutritional quality of corn and may, therefore, be able to solve the problem of malnutrition
Effect of electromagnetic field on whole blood, biochemical and hormone level in human
Get PDFThe electromagnetic field is increasing in our environment and these exposures cause concern. The aim of our study is to investigate the effects of magnetic fields generated by the hairdryer devices used by women working in the same job on their serum biochemistry, whole blood, and hormone values. The sixteen women working continuously in hairdressing salons were included in the study. Two groups of studies were designed: control (n:8, mean age: 22.25±6.04) and experimental group (n:8, mean age:23,62±6.67). The biochemical (median values of alanine aminotransferase, aspartate aminotransferase, triglycerides, and very-low-density lipoprotein were found high) and hormonal results of the experimental group were compared with the biochemical (cholesterol, low-density lipoprotein, and very high-density lipoprotein had high median values) and hormonal results of the control group and no significant difference was found (p>0.05). When the whole blood parameters were examined, the white blood cells and mean platelet volume results of the experimental group were significant (p<0.05), while there was a meaningless difference between red blood cell, hemoglobin, hematocrit, mean corpuscular volume, red cell distribution width and platelet values (p>0.05). It is evident from the results that occupational exposure to magnetic fields constantly leads to changes in the biochemistry, hormone and whole blood parameters of the female
Chymase Level in Dengue Virus Infection With or Without Positive Non-Structural 1(NS1)
Get PDFThrombocytopenia, leukopenia, and monocytosis are laboratory parameters in the diagnosis of dengue virus infection. In addition to monocyte cells, mast cells also play a role in the innate immune response, where degranulation of mast cells will occur, which will secretion the active vaso mediator, Chymase. Chymase has a role in increasing vascular permeability resulting in plasma leakage in patients with dengue virus infection to determine the number of platelets, leukocytes, monocytes and chymase levels in patients with dengue infection in the acute phase. The platelet count mean in NS1 (+) was 132,140 cells / mm3 and the platelet count in the NS1 group was (-) 176,000 cells / mm3. The mean leukocytes NS1 (+) showed results of 4,350 cells / mm3 and NS1 (-) 5,250 cells / mm3. The mean monocyte NS1 (+) monocyte count was 8.26%, and NS1 (-) group was 8.76%. There were no significant differences in platelet counts, leukocytes and monocytes between NS1 (+) and NS1 (-) (P value> 0.05). The mean Chymase NS1 (+) 23.48, NS1 (-) 23.05 ng / mL and the control group 1.47ng / mL.ABSTRAK
Latar Belakang. Infeksi virus dengue menjadi masalah kesehatan di daerah tropis dan sub tropis di seluruh dunia, termasuk di Indonesia. Trombositopenia, leukopenia dan monositosis merupakan parameter laboratorium dalam penegakan diagnose pada infeksi virus dengue. Respon imun terbangun setelah nyamuk Aedes Aegipty yang terinfeksi menginokulasikan virus dengue ke tubuh manusia. Selain sel monosit, sel mast juga berperan dalam respon imun bawaan, dimana akan terjadi degranulasi sel mast yang akan mesekresikan mediator vaso aktif diantarnya adalah Chymase. Chymase mempunyai ikut berperan dalam meningkatnya permeabilitas vaskuler sehingga terjadi kebocoran plasma pada penderita infeksi virus dengue.
Tujuan. Untuk mengetahui jumlah trombosit, leukosit, monosit dan kadar chymase pada penderita infeksi dengue pada fase akut.
Metode. Penelitian ini adalah observasional analitik dengan pendekatan cross sectional. Penderita infeksi virus dengue diambil darahnya pada waktu masuk ke rumah sakit waktu mengalami demam pada hari kedua atau ketiga atau keempat. Darah langsung diperiksa darah lengkap serta NS1 rapid test. Serum penderita yang terdiagnosa DF maupun DHF dikumpulkan dan diperiksa kadar chymasenya.
Hasil. Rerata trombosit pada NS1 (+) 132.140 sel/mm3 dan jumlah trombosit pada kelompok NS1 (-)176.000 sel/mm3. Rerata leukosit NS1 (+) menunjukkan hasil 4.350 sel/mm3 dan NS1(-) 5.250 sel/mm3. Rerata monosit NS1(+) jumlah monosit 8,26% dan kelompok NS1 (-) 8,76%. Tidak terdapat perbedaan jumlah trombosit, leukosit dan monosit yang signifikan antara NS1(+) dan NS1(-) (P value >0,05)
Rerata kadar Chymase NS1(+)23,48, NS1 (-) 23,05 ng/mL dan kelompok control 1,47ng/mL.
Kesimpulan. Tidak terdapat perbedaan kadar chymase yang signifikan antara kelompok NS1(+) dan NS1(-). Perbedaan kadar chymase yang signifikan terdapat antara kelompok kontrol dengan NS1 (+) dan kelompok kontrol dengan NS1(-) pada infeksi virus dengue. Terdapat sedikit penurunan sel trombosit dan sel leukosit seiring dengan sedikit peningkatan jumlah sel monosit pada penderita infeksi virus dengue.
Kata Kunci: thrombositopenia, chymase, NS1, infeksi virus dengu
The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O
No full textBAP media is a medium used to distinguish pathogenic bacteria based on their hemolytic power on red blood cells. Staphyllococcus aureus is a bacterium that is able to emolate red blood cells with 3 types of hemolysis, namely α, β, γ, and δ. Usually BAP media is made by adding 5-10% sheep blood. Making BAP media using sheep blood has become a problem for several laboratories today, including health education laboratories. This is because the health education laboratory does not yet have a sheep farm, so it has not been able to procure sheep blood. The use of human blood as a substitute for sheep blood in making BAP media may be a solution, but it is not yet known whether there are differences in the growth and hemolysis of S. aureus bacteria on BAP media in sheep's blood and human blood. This research is an experimental study with a completely randomized design (CRD) of 3 replications which aims to determine whether there are differences in growth and hemolysis of bacteria S. aureus in BAP media of sheep blood and human blood groups A, B, AB, and O. The results showed that S. aureus bacteria could grow and show hemolysis in BAP media in sheep blood and human blood in groups A, B, AB, and O. The results of subsequent studies analyzed ANOVA using the software spss for windows with a significant level of 0.05. From the results of research and data analysis it can be concluded that S. aureus bacteria can grow and show hemolysis in BAP media of sheep blood and human blood groups A, B, AB and O, but there are significant differences in the number of S. aureus bacteria colonies grown in BAP media of sheep's blood and human blood groups A, B, AB and O
The concentration of NaCl soaking to decreasing cyanide levels in Gadung (Dioscorea hispida Dennst)
No full textCyanide is one component contained in gadung tubers, and therefore, consuming gadung that contains more than 1 ppm of cyanide can cause nausea and intoxication. This research aims to study the effect of concentration variation of NaCl soaking to the decrease of cyanide levels in gadung. This research is an experimental study with three treatment. Testing is only done once. Samples of gadung were soaked by NaCl (0% 1% 3% 5%) for 3 days. Determination of cyanide content in gadung used the UV-Vis spectrophotometry method by adding Ninhydrin 0.1% in Na2CO3 2% and NaOH 0.1 % in the sample filtrate. Result of this research had shown that cyanide content in gadung before NaCl soaking was 4.42 ppm and after NaCl soaking (0% 1% 3% 5%) were 3.52; 2.53; 0.43 and 0.01 ppm, respectively.Umbi gadung termasuk tanaman umbi yang dapat dimanfaatkan sebagai pengganti makanan pokok. Sianida merupakan salah satu komponen yang terdapat dalam umbi gadung. Mengkonsumsi umbi gadung yang mengandung sianida lebih dari 1 ppm dapat menimbulkan rasa mual, dan mabuk. Tujuan penelitian ini adalah untuk mengetahui pengaruh variasi konsentrasi perendaman NaCl terhadap penurunan kadar sianida pada umbi gadung. Penelitian ini mengunakan sampel umbi gadung dengan perlakuan perendaman NaCl (0%, 1%, 3%, 5%) selama 3 hari. Metode yang dipakai adalah spektrofotometri UV-VIS dimana filtrat sampel ditambahkan Ninhidrin 0,1% dalam Na2CO3 2% dan NaOH 0,1%. Hasil penelitian menunjukkan bahwa kadar sianida pada umbi gadung sebelum perlakuan perendaman NaCl adalah 4,42 ppm dan setelah direndam NaCl (0%, 1%, 3%, 5%) didapatkan hasil berturut- turut sebesar 3,52; 2,53; 0,43 dan 0,01 ppm
The potency of bay leaves extract (Syzygiumpolyanthum) as anti-inflammation in Rattus norvegicus induced with Complete Freund’s Adjuvant
Get PDFThe using of herbal plants in Indonesia as medicine based on traditional knowledge. One of the herbal plants that believed has medication effect is bay leaves (Syzygiumpolyanthum). Bay leaves contain a flavonoid that can prevent inflammation. The study aims to prove the potency of bay leaves extract to prevent the inflammation, which is indicated by decreasing the paw edema thickness in rat induced with Complete Freund's Adjuvant (CFA). This study used 25 animal models of male rat (Rattus norvegicus) strain Wistar that divided into five groups, one group for a normal group (no treatment), one group for positive control which was injected with CFA sub plantar and given treatment with Na CMC and three test groups which is got three different doses of bay leaves extract peroral and the doses were 100 mg/kg BW, 300 mg/kg BW, 400 mg/kg BW, and injected with CFA into the sub plantar. Bay leaves extract was given one hour per oral before injected with CFA. The measurement of paw edema was done at 24 hours after CFA injection. The result of this study showed that bay leaves extract gave the anti-inflammation effect to the rats which were injected with CFA. It is concluded that bay leaves extract containing flavonoids plays a role in inhibit cytokines proinflammatory synthesis.Penggunaan tanaman herbal sebagai pengobatan di Indonesia masih berdasarkan pada pengetahuan tradisional. Salah satu tanaman herbal yang dipercaya memiliki efek pengobatan adalah daun salam (Syzygium polyanthum). Daun salam mengandung flavonoid yang dapat mencegah inflamasi. Tujuan dari penelitian ini adalah untuk membuktikan potensi dariekstrak daun salam untuk mencegah inflamasi yang diindikasikan dengan penurunan ketebalan bengkak telapak kaki tikus yang diinduksi Complete Freund's Adjuvant (CFA). Penelitian ini menggunakan 25 ekor tikus jantan strain Wistar sebagai hewan coba yang dibagi dalam 5 kelompok. Satu kelompok adalah kelompok normal tanpa perlakuan, satu kelompok adalah kelompok kontrol positif yang diinjeksi CFA subplantar dan diberikan terapi dengan Na CMC dan tiga kelompok lain diberikan terapi ekstrak daun salam per oral dengan dosis masing-masing 100mg/kgBB, 300mg/kgBB dan 400mg/kgBB dan kemudian diinjeksi CFA subplantar. Pengukuran ketebalan edema pada telapak kaki tikus dilakukan setelah 24 jam injeksi CFA. Hasil dari penelitian ini menunjukkan bahwa ekstrak daun salam memberikan efek antiinflamasi pada tikus yang diinjeksi dengan CFA
RETN rs3745368 polymorphism and resistin level in Javanese ethnic Indonesian obese: a case control study
No full text
Obesity has become a global public health problem. It occurs due to a positive energy balance leading to adipose tissue expansion. White adipose tissue was an endocrine organ which secreted resistin. Resistin also produced by immune cells due to low chronic level inflammation might cause higher resistin level in obese people. Polymorphism +62G>A RETN gene was reported has a relationship with low resistin level and A allele as a protective allele. This study aimed to determine genotype and allele frequency distribution concerning resistin level. Another objective aimed to know the correlation between resistin level with body mass index. The design of the research was a case-control study with 122 people (18-40 y.o.), divided equally in the case group (BMI ≥ 27 kg/m2) and control group (BMI 18.5-24.9 kg/m2) without diabetes mellitus. Blood was taken after fasting a minimal 8 hours. Plasma was used to measure the resistin level. DNA genotyping was analyzed using PCR-RFLP. Genotyping result showed three genotypes of RETN gene +62G>A polymorphism (GG, GA, AA). There was no significant difference in genotype and allele frequency distribution related to obesity status (p=0.680; p=1) and resistin level (p=0.537) between case and control group. There was no significant difference in resistin level between case and control group (p=0.770). Resistin level was correlated with BMI in obese group (p= 0.05; r= -0.25). The present study concludes that there is no significant difference in genotype and allele frequency distribution related to obesity status and resistin level. Resistin level has a negative correlation with BMI
Erythrocyte sedimentation rate in diabetic and non-diabetic patients of cardiovascular disease
No full textThe pervasiveness of global endemic of type II diabetes, driven by population development, increasing obesity, urbanization, and other additional unidentified factors may be crumpled in the next 20yrs. The purpose of this study was to determine the correlation of ESR in diabetic and non-diabetic patients of cardiovascular diseases. It is a co-relational study conducted at KRL hospital Islamabad from February 2018 to September 2018. The study population compromised a total of 180 cases aged 45 to 50 years were taken, in which 60 cases of CVD with DM, 60 cases of CVD without DM and 60 normal healthy population were taken. All patients and controls gave written informed consent. All subjects went through a general physical examination, and a questionnaire was used to collect the records about demographics and past medical history and existing use of medications. In the presented study, Pearson's Correlation between ESR of healthy and ESR of CVD without DM shows a weak correlation between these two variables i.e., 0.127 with a p-value of 0.503. We also establish that there is a direct intermediate relationship between ESR of healthy and ESR of CVD with DM. High level of ESR in CVD patients might indicate the prognosis of DM. To confirm this further researches and studies must be conducted in this are