Ilmu Pertanian (Agricultural Science)
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    The Effect of Drying and Storage on the Quality of Shallot (Allium cepa L. Aggregatum group) Bulbs

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    Post-harvest handling in shallot such as drying of bulbs can influence its quality during and after storage. The objective of this study was to determine the impact of drying and storage treatment on the quality of shallot bulbs during 12 weeks of storage . The study was carried out in Samiran hamlet, Parangtritis village, Bantul district, Special Region of Yogyakarta and Crop Science Laboratory of the Faculty of Agriculture, Universitas Gadjah Mada, from June until November 2016. It was arranged in factorial randomized complete block design (RCBD) and consisted of two factors. The first was the drying treatments: drying the bulbs on the field and on woven bamboo nets both plastic covered and uncovered. The second was the storage treatments: storing the bulbs in the farmer’s warehouse (31,030C±0,04 and RH of 60,50%±0,28), in air-conditioned room (22,40oC± 0,02 and RH of 61,60%±0,09), and at room temperature (30,47oC±0,03 and RH of 60,50%±0,12). Each treatment combination was replicated three times as blocks. The results showed that water content of all treatments were changing followed by fluctuating of the total soluble solid throughout the storage period while bulb firmness tended to decrease. Bulbs which were stored in air-conditioned rooms showed the highest percentage of sprouted bulbs, vigor index and germination rate than other treatments. Meanwhile, drying treatment did not give significant influence.         

    Growth and Yield Response of Maize (Zea mays L.) on Acid Soil to Different Rates of Humic Acid and NPK Fertilizer

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    The main constraint in the extensification of maize on the dry land of acid soil is the low availability of P in soil. This study aimed to determine the effect and the optimal dose of humic acid on the growth and yield of maize on acid soil. This research was conducted from February to May 2017 at Tri Dharma Field Laboratory, Faculty of Agriculture, Gadjah Mada University. Acid soil with low P availability used was from sub-district Cigudeg and Jasinga, West Java. The research was arranged in Randomized Complete Block Design (RCBD) with 2 factors. The first factor was maize cultivar, namely Bisi 2 and Pioneer 35. The second factor was the rate of humic acid and NPK fertilizer, consisting of 0 kg.ha-1 (without) NPK and 0% (without) humic acid, NPK + 0% (without) humic acid, NPK + 5% humic acid, NPK + 10% humic acid, and NPK + 15% humic acid. NPK fertilizer applied was NPK 16:16:16 at a dose of 350 kg.ha-1. The results showed that humic acid application on acid soil increased C-humic content in the soil, soil P availability, total dry weight of the plant, and kernel dry weight at harvest (15 weeks after planting). The increase in soil P availability did not improve the plant growth but increased the accumulation of plant biomass.  The application of humic acid at 15% (52.5 kg.ha-1) combined with NPK fertilizer on acid soil significantly increased total dry weight of plant and kernel dry weight up to 13.14% and 21.81%, respectively, thus, it is recommended for maize cultivation on acid soil.

    Response of Morphological and Physiological Characteristics of 4 Flood- tolerant Sugarcane (Saccharum Officinarum L.) Cultivars to ZA (Zwavelzuur Ammoniac) fertilizer application in Jember and Bondowoso

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    The business expansion of sugarcane can be done using marginal land, such as wet land. This study attempted to get the right doses of ZA fertilizer applied to several sugarcane cultivars, which are tolerant to flooding in two different locations. The research was carried out in Jubung Agrotechnopark garden,  University of Jember and seed gardens of Dewisri Bondowoso, from November 2015 to November 2016. The experiment was arranged in factorial randomized complete block design. The first factor was the doses of ZA fertilizer, consisting of 3 levels of treatment, i.e. 0 kg.ha-1 (without ZA fertilizer), 500 kg.ha-1 and 1000 kg.ha-1. The second factor was 4 sugarcane cultivars,  consisting of flood-tolerant sugarcane cultivars from 2014 screening result, i.e. PSJT 941, Bulu Lawang, PS 865 and Kidang Kencana. The results showed that the application of ZA fertilizer at 1000 kg.ha-1 affected the height of stems, the number of tillers, diameter of the stem and the number of segments. Meanwhile, the application of ZA fertilizer at 500 kg.ha-1 affected the number of leaves, fresh weight of shoots, dry weight of shoots, fresh weight of roots, dry weight of roots, brix content, and the content of sucrose and reducing sugar. Flood-tolerant sugarcane cultivars are shown by the highest brix and sucrose fertilizer, observed in  PS 865, Bulu Lawang, PSJT 941, and Kidang Kencana, sequentially. The highest content of reducing sugar was found in PSJT 941, PS 865, Kidang Kencana, and Bulu Lawang, successively

    Effect of Salt Pretreatment on the Growth and Yield of Oryza sativa L. (cv. Dendang) under Saline Condition

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    Productivity of rice as staple food of Indonesia needs to be improved. One of the efforts is by utilizing saline area for agricultural land. Salinity can be a serious problem leading to a decrease in crop productivity. Plant adaptation under salinity is an alternative to lower the risk, which can be improved by seedling pretreatment. The experiment was conducted in Baros, Kretek, Bantul, Yogyakarta. The objective of this research was to determine the growth and yield response of rice cv. Dendang  to  the salt pretreatment at early stage. The experiment was arranged in completely randomized design. The treatments used were salt pretreatment and without salt pretreatment. The salt pretreatment increased K+ concentration, total dry matter, plant height and number of tillers. However, it decreased proline and Na+ concentration of leaf. There was no significant difference on the yield and yield component. Increasing EC values up to 8.35 dSm-¹ at generative phase reduced the rice ability to cope this level of salinity despite the application of salt pretreatment. The benefit of salt pretreatment was exhibited on rice grown under EC value 5 dSm-¹

    Characteristics of Soils Developing from Gabbro, Phyllite and Chert Parent Rock in Karangsambung District

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    Understanding the nature of the soil is very important to know the potential and the proper management of the soil. This study aimed to determine the differences in morphological, physical, and chemical properties of the soils developing from gabbro, phylitte and chert parent materials. The soil profile was made to represent each parent rock of gabbro, phyllite and chert located on the upper and middle slopes with pine-dominated vegetation and mixed gardens. Observation in the field is a professional description to observe soil morphology. Soil samples were taken at each horizon to analyze soil physical properties (bulk density, particle density, and texture), soil chemical properties (pH, exchanged cations, cation exchange capacity, available P, organic C, and total N). Texture analysis results showed that clay content of the soil developing from parent rock of Gabro 1 is the highest, followed by the soil clay content from  Chert 1, Phyllite 1, Chert 2, Phyllite 2, and Gabbro 2, respectively. The order of soil acidity level (pH) is Gabbro 2 > Gabbro 1> Chert 1 ~ Chert 2 > Phyllite 1 ~ Phyllite 2. Meanwhile, the order of the cation exchange capacity is Gabbro 1> Gabbro 2> Phyllite 1> Chert 1> Phyllite 2> Chert 2, and the order of the base saturation is Chert 2> Gabbro 2> Chert 1> Phyllite 2 > Phyllite1> Gabbro 1

    Effects of Different Types and Dosage of Green Mulch on Yield and Quality of Aloe vera L. on Coastal Sandy Soil

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    A complete content of chemical substance from Aloe vera leaf makes this plant has many functions such as the ingredient of functional food for health, cosmetics, and herbal medicines. This research was aimed to determine the yield and quality of Aloe vera L. on various types and rates of green mulch in coastal sandy soil. The research was conducted in coastal sandy soil of Poncosari Srandakan, Bantul, Yogyakarta and done in a Randomized Complete Block Design (RCBD) of two factors with three replications. The first factor was four types of green mulch (shrimp evergreen, cashew, acasia and gliricidia). The second factor was various rate of green mulch consisted of three levels rates of leave mulch (5.0, 10.0, and 15.0 tons ha-1). Soil without mulching was used as a control treatment. The variables observed were yield at the first harvest time and leaf content quality which includes water, protein, fat, carbohydrate, ash, fiber, and mineral (Ca, Fe and Zn). The results showed that there was interaction between types and rates of leave mulch in all variables observed. Gliricidia leaf mulch gave the best effect with optimum rate about of 10 ton ha-1. The effectiveness of green mulch abilities on improving the observed variables from higher order to low was gliricidia, acasia, cashew and shrimp-type evergreen with the rate range between 10 up to 15 tons ha-1

    Growth and Yield of Inpari 29 Rice Varieties on Raised-bed and Different Depths of Sunken-bed in Saline Field

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    Increased productivity of rice is required to meet the increasing food demand. Utilization of marginal lands, such as saline land is one of the solutions applicable to increase rice production. The objective of this study was to determine the growth and yield of Inpari 29 rice variety planted on raised-bed and different depths of sunken-bed in saline field. This study used the Split Plot Design with two treatments. The depth as the main plot consisted of two depth levels: a depth of 50 cm and a depth of 25; and the planting area field as the subplot that consisted of two levels: raised-bed and sunken-bed. The treatment was repeated three times. The rice planted in sunken-bed showed higher growth than in raised-bed as indicated by the high content of chlorophyll a, chlorophyll b, total chlorophyll, nitrate reductase activity and plant height. The rice planted in the sunken-bed yielded higher than in raised-bed as indicated by higher harvest index and the weight of grain per clump. Rice planted in 25 cm depth showed higher nitrate reductase activity and grain weight per clump than in 50 cm depth

    Genetic Diversity of Croton (Codiaeum variegatum (L.) Rumph.ex A. Juss) and Its Offspring Based on RAPD Markers

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    Croton (Puring) is a native  plant of Indonesia which has varied leaf shapes and colors. The diversity of croton increase through hybridization. The information on genetic diversity and relationship between parent and its offspring of crotons is very limited. This study aims to analyze the genetic diversity of cultivar Mawar (MW) and Walet (W) as parent compare to their offspring i.e, Black Marlet (BM), Kingkit 1 (KA), Kingkit 2 (KB), and Kamaratih (KM) using RAPD markers. This study used DNA extraction from the fresh leaf of six cultivars. The next steps were DNA quantification, primary optimization, DNA amplification with PCR, and electrophoresis. Statistical analysis was carried out using Genalex software. A total of 40 primers were screened, out of which 10 were selected for the analysis of genetic diversity. A total of 106 polymorphic bands were generated, ranging from 130 to 1850 bp. The results of RAPD analysis showed that Mawar as female parent had the highest polymorphic bands percentage of 69.01%, while Walet as male parent and its offspring ranged from 31.15 % to 43.94%. The genetic distance of the offspring with Walet ranged from 0.176 to 0.234 and genetic distance of the offspring with Mawar ranged from 0.314 to 0.372. It was indicated that all of offspring were closer to the male parent

    The Contribution of Calcium to Changes in Leaf Anatomical Character of Oil Palm Seedlings (Elaeis guineensis Jacq.) under Drought Stress

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    The research was purposed  (1) to know the effects of drought stress on changes in leaf anatomical character of oil palm seedlings (2) to know the contribution of calcium in cell compactness and increase the structural strength of leaf tissue so that oil palm seedlings were more tolerant to drought stress. This experiment was laid out following a split plot design with three blocks as replication. Main plot consists of drought stress levels, that are field capacity (FTSW 1.00), moderate drought stress (FTSW 0.35) and severe drought stress (FTSW 0.15). The fraction of transpirable soil water (FTSW) is a method for evaluating gradually increasing drought stress based on the amount of water loss due to transpiration. Meanwhile, subplot consists of four doses of calcium (Ca), that are of 0.0 g/seedlings, 0.04 g/seedlings, 0.08 g/seedlings, and 0.12 g/seedlings. Calcium fertilizer used is calcium sulfate (CaSO4) pure analysis. Leaf anatomical character was observed including the epidermal length and epidermal width; hypodermal length and hypodermal width; palisade cell length and palisade cell width; sponge cell length and sponge cell width; mesophyll tissue thickness; xylem and phloem diameter. The results showed that moderate and severe drought stress reduced epidermal cell length, upper hypodermal cell width, mesophyll thickness, palisade width and phloem diameter of leaf vessels. The applications of calcium to the leaf of oil palm seedlings under drought stresses were able to increased in the sponge cell length at a Ca dosage of 0.04 g/seedlings; increased lower hypodermal width and diameter phloem at a Ca dosage of 0.04 g/seedlings; and increased diameter xylem of leaves vessel at a Ca dosage of 0.12 g/seedlings

    Microsatellite Genetic Markers of Saccharrum spp., and Erianthus sp. on Their Hybrids

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    Progeny identification is the important step that should be done after hybridization. However, polyploidy, aneuploidy and the high chromosome segregation in sugarcane which results various phenotypic characteristics variation and environmental effects become limiting factors to identify the progenies based on morphological characteristic. Microsatellite as one of molecular marker which has codominance inheritance, multiallelic, abundant in the genome and does not influenced by environmental factor is the best tool to asses the crossing fidelity accurately. This research aimed to identify the possibility of genetic marker of Saccharum spp. and Erianthus sp. on their hybrid using microsatellite molecular marker. This study was carried out in Molecular Genetic laboratory, Indonesian Sweetener and Fiber Crops Research Institute (ISFCRI) Malang, from August 2016 to July 2017. Eighty-six (86) F1 intraspecific and interspecific progeny, three commercial sugarcane varieties (PSJT941, PS881 and VMC7616) and two wild types (S. spontaneum dan Erianthus sp.) were assessed genetically by three microsatellite markers. Identification of microsatellite genetic markers was conducted by comparing the visualization band results from electrophoresis of each male and female parent through their progenies. All primers could identify Saccharum spp. and Erianthus sp. genetic markers. There were one to eleven Saccharum spp. and Erianthus sp. genetic markers could be identified such as 2-11 PS881-specific alleles; 2-3 VMC7616-specific alleles; 1-5 PSJT941-specific alleles; two S. spontaneum-specific alleles and 1-2 Erianthus-specific alleles. These findings could be used as the advance genetic marker of microsatellite in sugarcane breeding to asses the cross fidelity

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    Ilmu Pertanian (Agricultural Science)
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