JURNAL PESISIR DAN LAUT TROPIS
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KARAKTERISTIK MORFOLOGI BAKTERI SIMBION SPONS MENYERUPAI Cribochalina sp DARI PERAIRAN MALALAYANG SULAWESI UTARA
Get PDFBacteria are found to be highly associated with various marine organisms, such as sponges. Sponges are known as the product of bioactive compounds. However, some of the compounds produced by sponges are obtained by the simbion-bacteria. Therefore this situation enables sponges simbion bacteria to play a major role in producing bioactive compounds that have been isolated from the sponge. The purpose of this study is to isolate and determine the characteristics of morphology of spongy simbion bacteria resembling Cribochalina sp, which was taken from Malalayang waters, North Sulawesi. Sponge simbion bacteria grow on NB media. Initially, a free-breeding colony was being done before bacteria were accrued in the NA media by 2% sponge broth (patent SID201906301) by means of Strike Plate methods. Based on to this study we had isolate five spongy simbion bacteria that resembling the Cribochalina sp. All five of these isolations have different characteristics of morphology in terms of color, shape, size, and elevation.      Keywords : bacteria, isolation, simbionts, spongesÂ
ANALISIS LOGAM TIMBAL (Pb) PADA Kappaphycus alvarezii (Doty) ALGA MERAH YANG DI BUDIDAYA DI TELUK TOTOK MINAHASA TENGGARA, SULAWESI UTARA
Get PDFMacroalgae are chlorophyll plants, consisting of thallus and can live in polluted waters. The purpose of the study was to analyze the lead metal (Pb) in the water column and thallus red algae Kappaphycus alvarezii which was cultivated in a certain period of time in the waters of Totok Bay. The cultivation method used is the bottom method. The algae weight is measured every two weeks. Water quality parameters and lead metal analysis (PB) are carried out in the Baristan laboratory. The results obtained are algae can grow according to the time period. Water quality can affect algae growth. Lead metal content in water is detected high enough as well as in thallus algae in the 3rd week. Keywords : Kappaphycus alvarezii, Lead metal, Water quality, Totok Bay Makroalga merupakan tumbuhan yang berklorofil dan tubuhnya berupa thallus serta mampu hidup pada perairan tersemar. Penelitian ini dilakukan dengan tujuan untuk menganalisis kandungan logam timbal (Pb) dalam air dan thallus alga merah Kappaphycus alvarezii yang dibudidayakan dalam periode waktu tertentu di perairan Teluk Totok. Metode budidaya pada yang digunakan adalah metode lepas dasar (bottom method). Pengukuran berat alga dilakukan setiap dua minggu. Beberapa parameter kualitas air dan analisis logam timbal (Pb) dilakukan di Laboratorium Baristan. Hasil yang diperoleh yaitu alga dapat tumbuh sesuai dengan periode waktu. Kualitas air dapat mempengaruhi pertumbuhan alga.Kandungan logam timbale pada air terdeteksi cukup tinggi demikian juga pada thallus alga di minggu ke-3. Kata kunci : Kappaphycus alvarezii, Logam Timbal, Kualitas air, Teluk TotokÂ
ISOLASI BAKTERI LAUT DARI PERAIRAN MALALAYANG, SULAWESI UTARA
Get PDFMarine bacteria have a lot of potential in exploring the enzyme that can be developed, such as a producer of proteorhodopsin, act as hydrocarbon chlorlastic and can degrade oil. This study aims to obtain isolates and can characterize the bacterial morphology. Malalayang Waters is one of the marine bacterial habitats that has potential area to be studied. This study aims to isolate marine bacteria from Malalayang Waters. These marine bacteria first were diluted into sea water before they were grown on Nutrient Agar (NA). Based on the results of this study it was found that marine bacterial isolated were separated based on their morphological characteristics. The dominant morphological characteristics were yellow whites which dominant shape were irregular.Keywords: bacterial, dilution, isolation. Bakteri laut memiliki banyak potensi yang dapat dikembangkan. Seperti penghasil proteorhodopsin, berperan sebagai hidrokarbonoklastik dan dapat mendegradasi minyak. Perairan Malalayang merupakan salah satu habitat bakteri laut yang belum diteliti. Penelitian ini bertujuan untuk mengisolasi bakteri laut dari Perairan Malalayang. Bakteri laut ditumbuhkan pada media agar + air laut, selain itu bakteri juga dilakukan pengenceran terhadap air laut sebelum bakteri ditumbuhkan pada media Nutrient Agar (NA). Berdasarkan hasil penelitian ini isolat bakteri  laut ditemukan, bakteri tersebut dipisahkan berdasarkan karakteristik morfologinya. Karakteristik morfologi yang dimiliki dominan berwarna putih kuning dan memiliki bentuk yang dominan tidak teratur. Hal ini dapat memperlihatkan perbedaan bakteri laut dari Perairan Malalayang yang tumbuh.Kata kunci: bakteri, pengenceran, isolasi
Identifikasi keanekaragaman lamun dan ekhinodermata dalam upaya konservasi
Get PDFLamun memegang peran penting di komunitas pesisir karena merupakan salah satu faktor pendukung dari berbagai macam flora dan fauna, mempengaruhi produktivitas perairan pesisir, penstabil sedimen mengontrol kejernihan dan kualitas air, serta dapat mempengaruhi ekosistem lain di sekitarnya. Tujuan dari penelitian ini adalah menganalisis keanekaragaman spesies lamun dan mengidentifikasi keanekaragaman ekhinodermata yang ditemukan di pesisir Kelurahan Tongkaina. Metode yang digunakan dalam penelitian ini yaitu metode Seagrass Watch. Data hasil penelitian, ditemukan lima jenis lamun, yaitu Cymodocea rotundata, Cymodocea serrulata, Thalassia hemprichii, Syringodium isoetifolium dan Enhalus acoroides yang terdiri dari dua famili yaitu Hydroccharitaceae dan Potamogentonaceae. Jenis lamun yang sering ditemui di Stasiun I adalah Cymodocea rotundata dengan nilai INP = 109,10 dan di Stasiun II Syringodium isoetifolium dengan nilai INP = 141,80. Enhalus acoroides adalah jenis lamun yang jarang ditemui pada kedua stasiun pengamatan, dengan nilai INP = 3,19 di Stasiun I dan di Stasiun II nilai INP = 52,30. Ditemukan tiga spesies ekhinodermata yang terdapat dalam transek kuadrat yaitu Protoreaster nodosus Ekhinothrix calamaris dan Linckia laevigata. Indeks keanekaragaman ekhinodermata di Stasiun I dengan nilai H' = 0,500 dan di Stasiun II dengan nilai H' = 0,410
STUDI PERUMUSAN STRATEGI PENGELOLAAN EKOWISATA BAHARI KOTA MANADO DI ERA REVOLUSI INDUSTRI 4.0 BERDASARKAN ANALISIS SWOT
Get PDFThe objective of this research was to formulate a strategy for managing maritime ecotourism in the era of industrial revolution 4.0 in the city of Manado. The study was conducted at the coastal areas of the mainland of Manado City and Bunaken Island for 3 months, February - May 2019. The research method was a survey with descriptive analysis and SWOT. The results of the study obtained 4 strategy formulations (key success factors) in the context of managing maritime ecotourism in the industrial revolution era 4.0 in the city of Manado, namely: 1) Increasing law enforcement in the field of marine ecotourism, waste management on land and sea, 2) Awareness of the community environmental hygiene both on land and sea, 3) Empowering biodiversity resources of coral reefs, seagrass beds and mangroves in the park for developing marine maritime ecotourism through digital applications, and 4) managing and developing resorts, coastal culinary attractions, points of diving spots, and tourism  ports. The results of the SWOT curve show the condition of marine ecotourism in the city of Manado in quadrant 2, namely a situation where the threat to the development of marine ecotourism is more dominant than opportunity, but there are strengths of tourism organizations that can be relied on. Stakeholders are expected to be able to improve performance so that quadrant 2 conditions change to quadrant 1, which is to support an aggressive strategy: a very good situation because of the power that is used to seize profitable opportunities. In the era of industrial revolution 4.0, every stakeholder in the maritime ecotourism industry in the city of Manado was supposed to change the management system towards digital-based by making applicationsKeywords: Bunaken island, revolution industry 4.0, Manado city, marine ecotourism, strategyTujuan penelitian adalah untuk merumuskan strategi pengelolaan ekowisata bahari di era revolusi industri 4.0 di Kota Manado. Penelitian dilakukan di wilayah pesisir daratan Kota Manado dan Pulau Bunaken selama 3 bulan, Februari - Mei 2019. Metode penelitian adalah survei dengan analisis deskriptif dan SWOT. Hasil penelitian diperoleh 4 rumusan strategi (faktor-faktor kunci keberhasilan) dalam rangka pengelolaan ekowisata bahari era revolusi industri 4.0 di Kota Manado, yaitu: 1) Meningkatkan penegakan hukum di bidang ekowisata bahari, pengelolaan sampah di darat maupun laut, 2) Menyadarkan masyarakat tentang kebersihan lingkungan baik di daratan maupun lautan, 3) Memberdayakan sumber daya keanekaragaman hayati terumbu karang, padang lamun dan mangrove di kawasan TNB untuk pengembangan ekowisata bahari melalui aplikasi digital, dan 4) Mengelola dan mengembangkan resort, tempat-tempat wisata kuliner pantai, titik-titik penyelaman, dan pelabuhan pariwisata.  Hasil kurva SWOT memperlihatkan kondisi ekowisata bahari Kota Manado berada dalam kuadran 2, yaitu situasi dimana ancaman terhadap pengembangan ekowisata bahari lebih dominan dibandingkan peluang, namun ada kekuatan organisasi kepariwisataan yang dapat diandalkan. Pemangku kepentingan diharapkan dapat meningkatkan kinerja agar kondisi kuadran 2 berubah menjadi kuadran 1, yaitu mendukung strategi agresif: situasi yang sangat baik karena adanya kekuatan yang dimanfaatkan untuk meraih peluang yang menguntungkan.  Dalam era revolusi industri 4.0, maka setiap pemangku kepentingan industri ekowisata bahari di Kota Manado sudah seharusnya merubah sistem pengelolaan yang ada ke arah berbasis digital dengan cara membuat aplikasi pengelolaan ekowisata bahari.Kata Kunci: Ekowisata bahari, revolusi industri 4.0, Kota Manado, Pulau Bunaken, strateg
Monitoring pertumbuhan mangrove di area modulasi karang dan mangrove Kampus Tateli Politeknik Negeri Manado
Get PDFResearch conducted in the Coral and Mangrove Modulation Area of the Tateli Campus of the Polytechnic of Manado State, in January to October 2019 aims to identify and differentiate the types of mangroves and then obtain data on mangrove growth in the Coral & Mangrove Modulation Area of the Tateli Campus. Retrieval of data using an area of 20 m2 of mangrove modulation and divided into four quadrants measuring 10 m2, while the tools used are: a compass to determine the direction or azimuth of research, a meter to measure distances., A photo camera for documentation tools, computers and other stationery which is used for data processing and writing research, rope, Tally sheet., Height measuring devices. Observations and data collection in this study included the measurement of trees, saplings, seedling height, seedling leaves, and seedling branches. From the existing mangrove modulation, four quadrants of mangrove observation were obtained. There are a number of mangrove individuals whose growth is observed and seen from the height, number of leaves and branches of the mangrove. Based on observations, it was found that in quadrant one there were two types of mangroves in the seedling phase, namely Rhizophora Apiculata and Rhizophora Mucronata with a total of 17 individuals. The average leaves of R. Apiculata are 7 strands. Then in the second quadrant two mangrove species were found, namely R. Apiculata and Sonneratia Alba, with a total of 15 individuals where the average height of Rhizophora Apiculata seedlings was 78cm, while Sonneratia Alba was 25.71cm. Furthermore, in this third quadrant only one species of mangrove Sonneratia Alba was found in the seedling phase. In this quadrant there are 15 mangrove individuals, with an average height value of 28.33 cm, an average value of leaves of 7.8 strands, an average value of 0.27 branches and the last in the fourth quadrant there are four types of mangrove seedlings namely R. Apiculata, R. mucronata, Brugueira Gymnoriza, and Sonneratia Alba. In this quadrant, 30 individual mangrove seedlings were found. Based on these results it can be concluded that from the existing mangrove modulation, obtained four quadrants of mangrove observation. There are several mangrove individuals whose growth is observed, as seen from the height, number of leaves and branches of the mangrove. Then the average mangrove growth is dominated by R. mucronata species. Whereas the highest rate of mangrove growth for 10 months is Brugueira Sp. Keywords: Mangroves, Growt
INVENTARISASI DAN KEPADATAN UDANG DAN KEPITING DI PERAIRAN MANGROVE
Get PDFPenelitian ini bertujuan mengetahui jenis-jenis udang dan kepiting, menentukan kepadatan, morfometrik dan mengetahui parameter kualitas air (suhu, pH dan salinitas) di perairan mangrove Kelurahan Meras Kecamatan Bunaken, Kota Manado. Hasil penelitian, diperoleh 4 spesies udang genus Penaeus dengan total 251 ekor (♂: 115 ekor dan ♀: 136 ekor). Kepiting yang didapatkan 10 spesies dari tiga genus yaitu Uca (295 ekor), Portunus (12 ekor) dan Scylla (53 ekor). Total kepiting yang ditemukan 360 ekor (♂: 203 ekor dan ♀: 157 ekor). Kepadatan tertinggi udang P. monodon, yaitu 0,092 ind/m2 sedangkan terendah P. merguiensis, yaitu 0,055 ind/m2. Kepadatan tertinggi kepiting U. annulipes, yaitu 0,126 ind/m2 sedangkan terendah P. trituberculatus, yaitu 0,003 ind/m2. Pengukuran morfometrik tertinggi udang P. monodon yaitu ♂: 19,9 cm, ♀: 17,8 cm sedangkan terendah P. semisulcatus yaitu ♂: 11,3 cm, ♀: 10,5 cm dan morfometrik kepiting tertinggi pada S. serrata yaitu ♂: 20,3 cm, ♀: 16,1 cm sedangkan terendah U. annulipes yaitu ♂: 3,7 cm, ♀: 2,4 cm. Hasil pengukuran parameter kualitas air, meliputi: suhu (27-30 0C), derajat keasaman (pH 6,9-8) dan salinitas (27-33 ppt), Kata Kunci : Inventarisasi; Cruise method; Udang; Kepiting; MangroveThe stock taking and solidity of shrimp and crab in mangrove waterThe purpose of this research is to describe the types of shrimp and crab, to decide the solidity, to decide the morphometric and to find out the parameter of water quality (temperature, pH and salinity) in mangrove water at Meras, Bunaken subdistrict, Manado. The result of this research found 4 species of shrimps genus Penaeus with total 251 (♂: 115 and ♀: 136 ). The founded crabs are 10 species from three genus, they are Uca (295), Portunus (12) and Scylla (53). The total of founded crab are 360 (♂: 203 and ♀: 157). The highest solidity of shrimp is P. monodon that is 0,092 ind/m2 , the lowest is P. trituberculatus that is 0,003 ind/m2 . The highest morphometric measurement of shrimp is P. monodon that is ♂: 19,9 cm, ♀: 17,8 cm while the lowest is P. semisulcatus that is ♂: 11,3 cm, ♀: 10,5 cm and the highest crab morphometric is S. serrata that is ♂: 20,3 cm, ♀: 16,1 cm while the lowest is U. annulipes that is ♂: 3,7 cm, ♀: 2,4 cm. The parameter measurement result of water quality, include: temperature (27-30 0C), acidity degree (pH 6,9-8) and salinity (27-33 ppt). Keywords: Stock taking, Cruise method, Shrimp, Crab, Mangrov
UJI AKTIVITAS ANTIBAKTERI BEBERAPA SPONS DARI PERAIRAN SALIBABU KEPULAUAN TALAUD
Get PDFABSTRAKSpons merupakan salah satu komponen terumbu karang yang berpotensi sebagai senyawa bioaktif namun belum banyak dimanfaatkan. Penelitian ini diarahkan untuk menperoleh beberapa ekstrak kasar spons dari perairan Salibabu Kepulauan Talaud, menentukan aktivitas antibakteri dari beberapa ekstrsk kasar spons serta membandingkan aktivitas antibakteri fraksi spons terhadap bakteri uji E. coli dan S. aureus. Hasil identifikasi spons ditemukan sebanyak tiga spesies yang terdiri dari: Siphonodictyon sp., Ircinia sp., Dysidea sp. Pengujian aktivitas antibakteri ekstrak kasar, fraksi air, metanol dan heksan menggunakan metode difusi agar dengan bakteri uji E. coli dan S. aureus. Ekstrak kasar siphonodictyon sp dan fraksi air dari ekstrak ini menunjukan aktivitas antibakteri tertinggi.Kata kunci : Spons, Antibakteri, E. coli, S. aureu
UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST
Get PDFABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT) Â
ISOLASI PIGMEN KAROTENOID DARI KEPITING Grapsus sp. (CAROTENOID PIGMENTS ISOLATION FROM CRABS Grapsus sp.)
Get PDFCarotenoids are pigments with a range of red, orange and yellow colors. In carotenoid and chlorophyll plants are located in chloroplasts which undergo photosynthesis as well as photosynthetic bacteria and fungi. Carotenoid compounds have antioxidant activity, anticancer, as precursors of vitamin A and can enhance immunity. Crustaceans like crabs Grapsus sp. have carotenoid pigments, visible red, orange, and yellow are scattered in the carapace. Pigments found in crustaceans as well as crabs of Grapsus sp. generally sourced from food eaten by the crab. To determine the process of carotenoid pigment metabolism, each crab organ of Grapsus sp. isolated using thin layer chromatography separation method and column chromatography to determine the type of pigment contained therein. Pigment types identified in the crabs of Grapsus sp. males using the TLC method: β-carotene, Astasen type, Cantasantin, Astasantin, and Adonirubin. Pigment types identified in the crabs of Grapsus sp. males using the CC method: β-carotene, Astasen, β-cryptosanthine, Zeaxantine, and Cryptosanthine. Keywords : Grapsus sp., Thin Layer Chromatography, Column Chromatography, Carotenoids Pigments.            Karotenoid merupakan pigmen dengan kisaran warna merah, orange dan kuning. Pada tumbuhan karotenoid dan klorofil terletak pada kloroplas yang mengalami proses fotosintesis seperti juga pada bakteri fotosintetik dan fungi. Senyawa karotenoid memiliki aktivitas antioksidan, antikanker, sebagai prekursor vitamin A dan dapat meningkatkan imunitas. Krustasea seperti kepiting Grapsus sp. mempunyai pigmen karotenoid, terlihat warna merah, jingga, dan kuning yang tersebar pada karapas. Pigmen yang terdapat pada krustasea demikian juga pada kepiting Grapsus sp. umumnya bersumber dari makanan yang dimakan oleh kepiting tersebut. Untuk mengetahui proses metabolisme pigmen karotenoid maka masing-masing organ kepiting Grapsus sp. diisolasi dengan menggunakan metode pemisahan kromatografi lapis tipis dan kromatografi kolom untuk mengetahui jenis pigmen yang terkandung didalamnya. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KLT yaitu : β- karoten, Tipe Astasen, Kantasantin, Astasantin, dan Adonirubin. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KK yaitu : β- karoten, Astasen, β-kriptosantin, Zeaxantin, dan Kriptosantin. Kata Kunci : Grapsus sp., Kromatografi Lapis Tipis, Kromatografi Kolom, Pigmen Karotenoid. Â