JURNAL PESISIR DAN LAUT TROPIS
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ISOLASI JAMUR SIMBION ASCIDIA Schizophyllum commune YANG MEMILIKI AKTIVITAS ANTIBAKTERI
Get PDFAscidian is a benthic invertebrate that produces secondary metabolites. The substances were produced by the ascidian as self-defense from many environmental factors. Several studies report the similar structure between the substances extracted from ascidian and symbiotic fungi with biological activities. This study aims to isolate fungi associated with ascidian Eudistoma sp., then observe its potency on inhibiting bacteria S. aureus dan A. hydrophila. PDA was used to isolate and culture fungal isolate, then extraction using solvent ethyl acetate. Antibacterial activity of this symbiotic fungi isolates extract was performed using the Kirby-Bauer disc diffusion method. Molecular identification of fungi results that the symbiotic fungi were Schizophyllum commune. The observing result showed that fungal extract has the ability on inhibiting the growth of S. aureus and with the inhibition zone 8 mm and A. hydrophila 8,5 mm.Keywords: Ascidian, Eudistoma sp., Schizophyllum commune, Symbiotic, Antibacteria
RUMPUT LAUT Eucheuma denticulatum SEBAGAI FITOREMEDIATOR PERTUMBUHAN UDANG VANAME (Litopenaeus vannamei)
No full textThe long-term goal of this research is the management of vaname shrimp culture that is certified "Good Fish Cultivation" (GFC) based on environmentally friendly and sustainable technology so that its products can be accepted in domestic and foreign markets. The specific target is the implementation of phytoremediation technology in shrimp aquaculture at the Marine Field Station owned by FPIK UNSRAT which will be developed into the North Sulawesi Marine Education Center (NSMEC) area. The long-term goals and specific targets above will support the leading areas of maritime and maritime affairs in the Unsrat Research Master Plan. Analysis of water quality parameters and shrimp growth response using controlled-scale phytoremediation technology was carried out to see the remediation ability of Eucheuma denticulatum seaweed. Research method using phytoremediation technology application on white shrimp cultivation in the form of demonstration plots. The average growth in the plots provided with seaweed ranged from 8.3 g to 8.4 g. While the control that was not stocked with seaweed, the average weight growth was 6.6 g. This figure shows quite different values for different treatments. Water quality parameters such as DO, pH showed better water quality conditions in the plots spread with seaweed. This shows that Eucheuma denticulatum seaweed is capable of being a phytoremediator for the environment where the vannamei shrimp (Litopenaeus vannamei) is present. Seaweed Eucheuma denticulatum has the ability to absorb waste such as the faces of white shrimp (Litopenaeus vannamei). Keywords: phytoremediation, seaweed, vaname shrimp, East Likupang
STRUKTUR KOMUNITAS IKAN TARGET DI EKOSISTEM TERUMBU KARANG PADA ZONA TRADISIONAL PULAU BUNAKEN, TAMAN NASIONAL BNAKEN
Get PDFThis study aims to determine the structure of the target fish community, including species composition, type of research and to analyze the ecological index in which the diversity index, uniformity index, dominance index, and community similarity index. Data collection was carried out in coral reef ecosystems in the traditional zones of Madolin, Alung Banua, Sachiko, Muka Kampung, and East Bunaken areas, all data collection areas were included in the Bunaken National Park area. Data was taken using the Underwater Visual Census method, also equipped with a documentation search for underwater photography and videography carried out at the Marine Biology laboratory, Faculty of Fisheries and Marine Sciences, UNSRAT. There are 3 families, 21 species and 130 individuals at the Madolin area, 5 families, 24 species, and 72 individuals at the Alung Banua area, 5 families, 23 species, and 77 individuals at the Sachiko area, 2 families, 11 species and 129 individuals at the Sachiko area. Muka Kampung, and 4 families, 18 species, and 160 individuals at the East Bunaken location. The calculated ecological index values obtained are H' 2.11 (Madolin), 2.90 (Alung Banua), 2.65 (Sachiko), 1.57 (Muka Kampung), 1.67 (East Bunaken) indicating the ecological condition of the ecosystem. coral reefs are still stable even though diversity is classified as moderate but inversely proportional to low dominance ranging from 0.07 to 0.33 on average. The results of the community similarity analysis using the Sorense index (<50%) showed that there was no similarity in reef fish communities between the 4 stations, namely (Madolin & Alung Banua), (Madolin & Muka Kampung), and (Muka Kampung & East Bunaken). Keywords : target fish community, diversity, dominance, community similarityÂ
FILOGENI MOLEKULER BAKTERI DARI MEDIA PEMELIHARAAN ROTIFER YANG DIBERI OLAHAN LIMBAH IKAN SEBAGAI SUMBER NUTRISI
Get PDFThis study aims to identify and construct molecular phylogeny of an isolate bacteria from culture media of rotifer Brachionus rotudiforis supplied with processed fishery waste feed as nutritional source. The use of fish waste-based food for rotifer showed positive effects on growth and nutrient content of the rotifers. Genomic DNA of the isolate bacteria BRLI- 01 was extracted and the 16S rRNA gene was amplified using primers (8F and 1492F) and further sequenced using Sanger sequence technique. The 16S rRNA gene was analysed using SeqScanner® and MEGA® followed with BLAST (Basic Local Alignment Search Tool) analyses in the NCBI (National Centre for Biotechnology Information). Amplification result of 16S rRNA gene bacteria s NCBI site as a reference for identification and phylogeny of bacterial species. BRLI-01 was successfully cultured on rotifer rearing media. The results of the 16S rRNA gene amplification of the isolate bacteria showed a DNA band with a length of 1400 bp. The BLAST result on the NCBI showed that the isolate bacteria BRLI-01 had a percent identity (98.46%) and is in the same phylogony branching position with Vibrio rotiferianus Keywords: Rotifers, Bacteria, Fish waste, 16S rRNA Genes, Phylogeny identificatio
PENAMBATAN MOLEKUL GLUTATION FAUNA LAUT TERHADAP RESEPTOR DARI BEBERAPA PENYAKIT VIRUS
Get PDFGlutathion is a tripeptides produced by almost all living creatures, including marine fauna such as rotifers and shrimps. Judging from the important molecular role of glutathion in living cell,  the ability of glutathion as antiviral compound, has been assed by application of the AutodockTools to predict the ability of glutathion as ligand to bind receptors of several viral diseases such as covid-19, malaria, and hepatitis B. Docking results of glutathion toward target receptor were shown by the parameter, Gibbs energy value, and supported by other data such as the amount of hidrogen bond, amino acid residues, and bond distance. Evidently, the glutathion could bind to each receptor of covid-19, malaria and hepatitis B, with values of  Gibbs energy (∆G), -7,9, 7,1 and -7,1 kcal/mol, respectively. This proves that glutathion has ability to bind covid-19 receptor, even stronger than its ability to bind receptors of other viral diseases, and to the remdesevir. Between glutathion and covid-19 receptor, there were 11 hidrogen bonds, with distance ranged from 2,76 to 3,25 Å. These were stronger than those between glutathion and receptors of malaria and hepatitis B, only  8 and 4 Å hidrogen bonds, respectively.  To develop glutathion as antiviral drug, it is necessary to carry out in vitro and in vivo analyses, prior to clinical test. Therefore, it requires to explore the marine fauna species that produce glutathion and their cultivation.Keywords: molecular docking, glutathion, marine fauna, autodock vina, viral diseases, binding affinit
POTENSI BIOAKTIVITAS ANTI JAMUR DAN ANTI-UV DARI ISOLAT JAMUR SIMBION PADA ASCIDIA Eudistoma sp
Get PDFAscidia Eudistoma sp. produce bioactive compounds that can inhibit the growth of microorganisms. Ascidians are also known for their potential secondary metabolites in the biomedical world. This study aimed to obtain isolates of the ascidia symbiont Eudistoma sp., determine the antifungal activity against the test fungus Candida albicans, and determine the anti-UV activity of the symbiont Eudistoma sp. The ascidia symbiont fungus was extracted using maceration method with ethyl acetate solvent and tested for antifungal activity using the test fungus Candida albicans. The antifungal activity test results showed that the diameter of the AFBN 5b inhibition zone of 9 mm. The symbiont fungus can be said to have potential as an antifungal drug. Testing the anti-UV activity of fungi in symbiosis with ascidia Eudistoma sp. using a spectrophotometer showed that the extract sample of the ascida symbiont fungus could produce an absorption at UV-B (λ 290–320 nm) of 3.8 absorbance and when compared to UV-A (λ 370–400 nm) an absorption value of 0.38 was obtained and is still classified as moderate Keywords: Ascidia, Eudistoma sp., symbionts, Antifungal and Anti-U
ESTIMASI KANDUNGAN KARBON BIOMASA PNEUMATOFOR Avicennia marina
Get PDFMangrove forests play an important role in mitigating the effects of global warming as mangroves can absorbs and stores carbon therefor, mangrove plants including A. marina can act as carbon sinks. The study aim is to estimate the carbon content contained in A. marina pneumatophore biomass. The data was taken b using line transect method. The number of research stations are 3 stations contain 3 plots (10 x 10 m2) for each station. Samples were taken at low tide by established 3 microplots (50 x 50 cm2) in each 10 x10 m2 plots. The results showed that the highest biomass value was found at Station 3 of 0.49 Kg/m2, followed by Station 1 of 0.42 Kg/m2, and the least pneumatophore biomass value was found at Station 2 of 0.41 Kg/m2.  This finding is in line to the potenntial potential carbon content in pneumatophores where Station 3 has C content (0.08 Kg C/m2), dand 0.07 Kg C/m2 for both station 1 and 2 respectively. The average of C Contain in pneumatphores was 0.28 Kg C/m2. Keyword : Carbon Content, Biomass, Pneumatophores, Avicennia marin
KELIMPAHAN DAN KEANEKARAGAMAN JENIS-JENIS KEPITING DI SUBSTRAT MANGROVE PERAIRAN MERAS DAN MOLAS KECAMATAN BUNAKEN KOTA MANADO SULAWESI UTARA
No full textCrab of the genus Uca is one type of crustacean that makes mangroves a habitat to carry out their life cycle in order to remain sustainable. The purpose of this research is to identify the first, the second is to calculate the abundance, species diversity, dominance, and the third is to know the state of the aquatic environment around pH, temperature, salinity and substrate. The method used is the cruising method and the quadratic line transect method. The method of roaming as far as 300 m. While the line transect method is carried out by drawing three transect lines along 28 m. The identification results in Meras waters found 6 species. Based on data analysis using the cruising method, the number of individuals in Meras waters is 383 individuals, while in Molas waters there are 210 individuals. Based on data analysis using the quadratic line transect method, the species abundance index in Meras waters is 6,767 ind/m2, while in Molas waters it is 4,533 ind/m2. The value of H' at two locations is moderate. The value of C in two locations is low.   Keywords: Meras, Molas, Crabs, abundance, species diversity, dominance
Keanekaragaman Makrobentos yang Menempati Agregasi Kerang, Septifer bilocullaris di Tiwoho, Kabupaten Minahasa Utara, Sulawesi Utara
Get PDFThe purpose of this study was to determine the type and diversity of macrobenthos that inhabit the aggregation of Septifer bilocularis shellfish in the coastal waters of Tiwoho, North Minahasa Regency, North Sulawesi. Sampling of macrobenthos was carried out using core (PVC), both at the position of small aggregation (AK), middle large aggregation (ABT), and large edge aggregation (ABP). Sampling was carried out 4 times on different aggregations of shellfish. The data has been analyzed using the Diversity Index (H), the dominance index (D), and the Uniformity Index (E). It was identified that there were 25 types of macrobenthos occupying all aggregations of septifer bilocularis shells, which came from 11 classes including Gastropods, Bivalves, Malacostraca, Florideiphyceae, Ulvophyceae Ophiuroidea, Clitelatta, Maxillopoda, Globothalamea, Tubothalamea and Phaeophyceae. Biota diversity index varies from 1.60 – 1.70 which indicates that the biota in each aggregation has moderate diversity. The uniformity index value also varied, namely 0.069 for macrobenthos occupying the small aggregation (AK), then 0.066 in the middle large aggregation (ABT) and 0.064 in the large margin aggregation (GBP), which indicates the low uniformity of the macrobenthos in each aggregation. The dominance index of all aggregations was obtained between 0.27 - 0.36, which indicates that there is no species dominance for macrobenthos occupying different shellfish aggregations.Keywords: Diversity, Macrobenthos, Septifer bilocularis, Tiwoh
Ekstraksi Pigmen Klorofil Total Pada Mikroalga Dunaliella sp. Yang Telah diberi Perlakuan Timbal Asetat
Get PDFHeavy metals such as lead are compounds that can cause toxic effects if they enter the body of living things. Dunaliella sp. is one of the marine organisms that is susceptible to changes or ecological pressure so that it is the main target of being exposed to pollutants such as heavy metals and so on, so it is necessary to conduct research to determine how the effect of lead acetate compounds on the growth and total chlorophyll content of Dunaliella micro algae. sp. The results of this study indicate that the growth of Dunaliella sp microalgae on the control culture media and the lead treatment media has a very different growth, the control media has normal growth, while the total chlorophyll content of the 14th day in the control media is 80.49 µg / ml. 30 ppm 54.79 µg / ml, 50 ppm 50.02 µg / ml and 100 ppm 9.13 µg / ml. While the total chlorophyll content of the 30th day in the control media was 34.99 µg / ml, 30 ppm 44.657 µg / ml, 50 ppm 26.136 µg / ml and 100 ppm 5.58 µg / ml.Keywords : Microalga, Dunaliella sp, kcorophyll, lead ecetate   Â