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Stability-Indicating Methods for the Determination of Gemifloxacin in Presence of its Acid Degradation Product(s)
No full textBrilliant, valid and simple five UV spectrophotometric stability indicating techniques are adopted for the determination of Gemifloxacin (GEM) in presence of its acid degradation products over a concentration range of 2-12 μg mL . The first method is an application of the first derivative ( 1 1 , Amr M. Badawey 1,2 , Hebatallah M. Essam 1 and Fatma M. Aboul Alamine Cairo University, Faculty of Pharmacy, Analytical Chemistry Department, Kasr el Aini St, Cairo, Egypt 2 -1 D) spectrophotometry, that allows the determination of GEM without interference of its acid degradation products at zero crossing wavelength (254.6 nm). The second method depends on the first-derivative of the ratio spectra spectrophotometry ( DD) for determination of GEM in presence of its acid degradation products at a maximum of 273.0 nm and a minimum of 284.0 nm, While the third dual wavelength method offers a superior stability indicating procedures for the determination of GEM in the zero order spectra at the wavelength pair of 271.8 nm and 325.0 nm. The fourth method is the ratio difference one, with the advantages of minimal data processing and wide range of application. It is applied for the analysis of intact drug in presence of its acid degradation products by measuring the difference in the peak amplitude at the ratio spectra at 355.0 nm and 270.0 nm. The last method is based on the quantification of GEM through the bivariate calibration at 255.0 nm and 277.0 nm by adopting simple mathematic algorithm that provides simplicity and rapidity
A promising stem cells strategy to correct corneal defects due to a unique homozygous missense mutation p.R311K in the Tp63 gene causing dominant EEC syndrome.
No full textBackground Ectrodactyly - Ectodermal dysplasia - Clefting (EEC) syndrome is a rare autosomal dominant inherited disease. To date, approximately 40 different Tp63 mutations have been identified, all reported as heterozygous mutations. There is no definitive cure available to counteract and resolve the progressive corneal degeneration resulted from the premature ageing of limbal epithelial stem cells (LESC). However, oral mucosal epithelial stem cells (OMESCs) represent a valid alternative capable of regenerating the corneal epithelium and provide an attractive therapeutic avenue. Our group has recently discovered R311K-∆Np63 mutation, a unique homozygous mutation causing EEC syndrome with a mosaic pattern. The survival of the patient was intriguing as homozygous dominant mutations are lethal. Unlike other EEC mutations, R311K-∆Np63 keeps the binding ability to DNA that can make it a milder mutation if raised in heterozygous form. Aim of research The research focus on studying the R311K-∆Np63 mutation, its effect on protein expression, its transactivation activity and comparing it with other heterozygous mutations (R311G-, R304Q- and R279H∆Np63). The final goal of the research was to find a therapy for the ocular impairment of our EEC case by isolating and expanding ex vivo the heterozygous epithelial stem cells, identified by means of DNA and RNA characterization, from OMESCs biopsy. Cultured autologous OMESCs can represent an effective treatment to reconstruct the ocular surface and partially restore the ocular function in our case. Materials and methods R311K-∆Np63 mutation was studied by testing their protein expression in transfected HEK293T cells by western blot and by measuring their transactivation activity by Luciferase reporter assay on K14 promoter, as it contains p63 binding sites and because the protein expression is ∆Νp63 dependent. Individual cells were isolated from a primary cell culture obtained of OMESCs biopsy from the patient by clonal analysis, expanded ex vivo to be used for CFE analysis and molecular characterization. DNA was extracted with Chelex resin, purified by ethanol precipitation and amplified by PCR to be sequenced by Sanger Method. RNA was extracted by Triazol, purified from possible DNA contaminants by RNAeasy (Biorad microkit), quantified, tested for integrity and quality by agarose gel electrophoresis, reverse-transcribed to cDNA, and then amplified to be analyzed by absolute real time PCR. Results The expression of R311K∆Np63 is increased in ectopic system of expression and Luciferase reporter assay revealed a severe inhibition in the transactivation activity of the K14 promoter in case of homozygously mimicking conditions in transfected HEK293T cells and a significant less severe inhibition in the transactivation activity of the same promoter in heterozygously mimicking transfected cells. Only 20% of isolated single cells of the primary cell culture of the OMESCs biopsy were able to grow into full colonies. These colonies were found heterozygous for the mutation R311K∆Np63. CFE assay for all colonies revealed the presence of 24 holoclones that, when analyzed by qPCR analysis, showed similar ∆Np63α expression level to LESC (previously used cells to successively correct limbal stem cell deficiency- LSCD) suggesting the possibility to use these holoclones to correct LSCD in our case. Conclusion Cultured autologous OMESCs, obtained by selected R311K-N∆p63 heterozygous stem cells, could be effective as personalized therapy to reconstruct the ocular surface of this particular and unique case of EEC syndrome
MATLAB : an introduction with applications / Amos Gilat, Department of Mechanical and Aerospace Engineering, The Ohio State University.
No full textIncludes index.x, 406 pages
Using NLP Approach for Opinion Types Classifier
No full textInformation that are represented as text are either facts or opinions, whenever we need to make a decision, we often seek out the opinions of others which is one of the most influencing factors for our decisions. Traditionally, individuals can get opinions from friends and family while organizations use surveys, focus groups, opinion polls and consultants. Nowadays, opinions expressed through user generated content are considered as one of the important types of information which is available on the web, therefore, many resources have been emerged for expressing opinions including social media and others. This situation has revealed the necessity for robust, flexible Information Extraction (IE) systems, these systems have the availability to transform the web pages into program-friendly structures such as a relational database to reveal these opinions. In this paper, we propose an approach to classify the opinions of a document or a set of documents considering an object. The approach has been implemented and applied on a dataset of opinions. The proposed system discover the opinions provided for an object in a document or set of documents. The system discovers different types of opinionated statements, including the opinionated, comparative, superlative, and non- opinionated. The system has been applied on a set of 4000 sentences, and the results has been evaluated using the standard metrics, they are True positive, True negative, False positive, False negative, Precision, Recall, and F-score. We also provided a comparison of the presented work with previous work that has been presented in the same field
Cloud computing with e-science applications / edited by Olivier Terzo, ISMB, Turin, Italy, Lorenzo Mossucca, ISMB, Turin, Italy.
No full textcomputer bookfair2016Includes bibliographical references and index.xx, 300 pages :"The amount of data in everyday life has been exploding. This data increase has been especially significant in scientific fields, where substantial amounts of data must be captured, communicated, aggregated, stored, and analyzed. This book describes how cloud computing can significantly improve data management in different scientific fields, such as bioinformatics, earth science, and computer science. This book focuses on how cloud computing helps in the areas of resource control, vertical and horizontal scalability, and adaptive scheduling"-- Provided by publisher
Chemistry : |b the central science / Theodore L. Brown , H.eugene leMay, Bruce e.bursten , Catherine j.murphy , Patrick m.woodward , Matthew w.stolzfus.
No full textIncludes index.1 volume (various pagings)
Dietary Chinese herbs : chemistry, pharmacology and clinical evidence / Yanze Liu, Wang Zhimin, Junzeng Zhang, editors.
No full textpharmacy bookfair2016Includes bibliographical references and index.xxvi, 802 pages :This work presents up-to-date information on chemical, pharmacological, clinical studies and historical uses of common dietary Chinese herbs. A brief chronological review of Chinese literatures on dietary herb uses, with chapters dedicated to each selected dietary herb, and with plant Latin name indices is provided. Examined are the health benefits that have been studied for centuries. A wide range of Traditional Chinese Medicine (TCM) herbs are investigated for their suitability into daily diets for maintaining general wellness or disease prevention. In the past decade, natural health products, dietary supplements, functional foods, or nutraceuticals have emerged in the West due to the increasing demand for non-pharmaceutical healthcare products. Traditional Chinese Medicine disease prevention and treatment incorporates the use of foods, and herbal medicine in an integrated manner, and thus the dietary Chinese herbs in used in TCM for thousands of years could be sources for developing new, effective, and safe ingredients to capture the rapidly expanding opportunity in the global market place
PHP and MYSQL web development : a beginner's guide / Marty Matthews.
No full textIncludes indexcomputer bookfair2016xxi, 568 p. :"This step-by-step tutorial shows you how to use PHP and MySQL to power websites with dynamic content. PHP and MySQL Web Development: A Beginner's Guide takes you from building static pages to ones with PHP and MySQL dynamic elements. Hands-on projects get you started using new skills right away!This solutions-oriented book is organized around real-world web scenarios that demonstrate the use of PHP and MySQL. These scenarios can be directly used to build web pages with dynamic content. Security topics such as validation, authentication, and encryption are addressed throughout. Each chapter teaches practical techniques that can be immediately applied Covers the basics of what drives many of today's most popular sites Ready-to-use code examples from the book available for download "-- Provided by publisher
High performance parallelism pearls : multicore and many-core programming approaches / James Reinders, Jim Jeffers.
No full textElectronic reproduction. Palo Alto, Calif. : ebrary, 2014. Available via World Wide Web. Access may be limited to ebrary affiliated libraries.computer bookfair2016Includes indexes.Previously issued in print: 2014.xlv, 502 pages
Basic principles of drug discovery and development / Benjamin E. Blass, Temple University School of Pharmacy, Moulder Center for Drug Discovery Research, North Broad Street, Philadelphia, PA, USA.
No full textpharmacy bookfair2016Includes biblographical references and indexes.xv, 574 pages