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Direct Detection of Burkholderia cepacia in Susceptible Pharmaceutical Products Using Semi-Nested PCR
No full textBurkholderia cepaciahas recently received a considerable attention as one of the major risks in susceptible pharmaceutical products. This microorganism can easily propagate and cause vast and severe contamination, especially to the water supplies for pharmaceutical companies. Moreover, it proliferates within the products and can cause severe infections for humans. Therefore, fast and sensitive detection of these bacteria is of a great demand. The present study introduces improved application of a polymerase chain reaction assay with relatively high sensitivity and specificity for the direct detection ofB. cepaciafrom the aqueous pharmaceutical products. A semi-nested polymerase chain reaction approach using the primer set BCR1/BCR2 followed by BCR1/Mr yielding a 465 bp fragment of the recA gene was applied and tested using both crude lysate from isolated colonies and DNA directly extracted from artificially prepared and spiked reference syrup. The polymerase chain reaction assay showed no interference with other bacterial reference and environmental strains tested, includingStaphylococcus aureusATCC® 6538,Pseudomonas aeruginosaATCC® 9027,Escherichia coliATCC® 8739,Salmonella abonyNCTC® 6017,Bacillus subtilisATCC® 6633,Micrococcus luteus, Staphylococcus warneri, Pseudomonas fluorescens, Pseudomonas putida, andRalstonia pickettii Moreover, this semi-nested assay showed a detection limit of around 10 colony-forming units per sample and could detectB. cepaciastrains isolated from a municipal pre-treated potable water tank. Comparing the results for detection ofB. cepaciain 100 randomly collected commercial syrup preparations using both conventional standard method and polymerase chain reaction assay revealed thatB. cepaciawas detected in two samples using polymerase chain reaction assay while all samples showed negative results by conventional culturing and biochemical methods. These results highlight the advantage of using this polymerase chain reaction assay to detectB. cepaciain contaminated pharmaceutical products and even water for pharmaceutical purposes, without the need of culturing or pre-enrichment, where it may give false-negative results and may be misidentified when biochemically tested
Toxicology and Applied Pharmacolog
No full textCarbonic anhydrase inhibitors have emerged in the past few years as an
interesting candidate for the development of novel unconventional strategies. Despite
their effect in tumor regression via inhibition of tumor acidification, their potential role is
not yet fully elucidated. Herein, we investigated whether Acetazolamide (AZ) could
modulate Imatinib (IM) anticancer activity, both in breast cancer cells (T47D) and in
isolated tumor specimens of Ehrlich ascites carcinoma (EAC). The impact of this
combination on angiogenesis was evidenced by decreasing PDGF-A expression and
enhancing that of TSP-1. In the meantime, AZ significantly suppressed IM-induced
attenuation of VEGF secretion in T47D cells, most probably due to NO inhibition. The
combination also dramatically decreased the metastatic activity of T47D cells by
mitigating the protein levels of MMP-2, -9 and phosphorylation of p38 MAPK, while
increasing the expression of TIMP-1 and -2. In addition, a strong proapoptotic effect was
observed in T47D cells after combining AZ and IM in terms of increased caspase-9 and -
3 activities. Interestingly, these results were confirmed by the reduction in the isolated
tumor volume, MVD, Ki-67 and VEGF expression. Eventually, the study provides a new
therapeutic strategy for treating cancer
Therapeutic Potential of Bone Marrow Derived Mesenchymal Stem Cells in Modulating Astroglyosis of Surgical Induced Experimental Spinal Cord Injury
No full textBackground: Spinal cord injury (SCI) unsuccessful regeneration was due to glial scar development. It was a major obstacle to axonal restoration. Safe therapeutic intervention by the use of bone marrow derived stem cells (BMMSCs) transplantation applied in the present study could reduce spinal disability. Material and methods: Forty male albino rats were divided into four groups: GI: negative control (n = 10 rats); GII: positive control after SCI (n = 10 rats); GIII: SCI + BM − MSCs intravenous injected and GIV: SCI + BM − MSCs intra lesion injected (n = 10 rats in each group). The samples were taken from spinal cord tissues around the region of injury and were subjected to histological, immunohistochemical assessment. RNA extraction and real time PCR for detection of nerve regeneration and astrocyte response to the injury were also performed. Results: Clinical improvement occurred by the enhancement in the Basso, Beattie and Bresnahan (BBB) score after SCI. Histological examinations showed positive regenerative responses in GIV compared to GIII. Conclusion: BM-MSCs transplantation has a promising role in enhancing the microenvironment for nerve regeneration through stumbling the glial scaring formation and inflammatory response af- ter chronic spinal cord injury especially by using intra-lesion route injection
Nanoparticles as tool for enhanced ophthalmic delivery of vancomycin: a multidistrict-based microbiological study, solid lipid nanoparticles formulation and evaluation
No full textContext: A microbiological multidistrict-based survey from different Egyptian governorates was conducted
to determine the most prevalent causative agents of ocular infections in the Egyptian population. Antibiotic
sensitivity testing was then performed to identify the most potent antimicrobial agent. Vancomycin (VCM) proved
the highest activity against gram-positive Staphylococcus bacteria, which are the most commonly isolated causative
agents of ocular infection. However, topically applied VCM suffers from poor ocular bioavailability because of its
high molecular weight and hydrophilicity. Objective: the aim of the present study was to develop VCM-loaded solid
lipid nanoparticles (SLNs) using water-in-oil-in-water (W/O/W) double emulsion, solvent evaporation technique to
enhance ocular penetration and prolong ophthalmic residence of VCM. Method: Two consecutive full factorial
designs (24
followed by 32
) were adopted to study the effect of different formulation and process parameters on SLN
formulation. The lipid type and structure, polyvinyl alcohol (PVA) molecular weight and concentration, sonication
time, as well as lipid:drug ratio were studied as independent variables. The formulated SLN formulae were
evaluated for encapsulation efficiency, particle size, and zeta potential as dependent variables. Results: The
statistically-optimized SLN formula (1:1 ratio of glyceryltripalmitate:vancomycin with 1% low molecular weight
PVA and 1 min sonication time) had average particle size of 277.25 nm, zeta potential of -20.45, and 19.99% drug
encapsulation. Scanning and transmission electron micrographs showed well-defined, spherical, homogenously
distributed particles. Conclusion: The present study suggests that VCM incorporation into SLNs is successfully
achievable; however, further studies with different nanoencapsulation materials and techniques would be valuable
for improving VCM encapsulation
Future Dental Journal Volume 2, Issue 1
No full textArticles : Temporary unilateral hypoglossal nerve palsy secondary to infectious mononucleosis: A case report -- Full mouth implants rehabilitation of a patient with ectodermal dysplasia after 3-Ds ridge augmentation. A clinical report -- Quantitative analysis of metallic artifacts caused by dental metallic restorations: Comparison between four CBCT scanners -- Capsaicin induced histological and ultrastructural changes in the submandibular salivary gland of albino rats -- Collagen turnover induced by cellular connective tissue cytokines of drug induced gingival overgrowth and hereditary gingival fibromatosis (Histological and immunohistochemical comparative study) -- Comparisons among three supplementary irrigation techniques and a calcium hydroxide dressing for bacterial elimination after chemomechanical preparation using the self-adjusting file -- Effect of different surface treatments on bond strength, surface and microscopic structure of zirconia ceramicFuture Dental Journal (FDJ) publishes scientific articles, case reports and comparison studies evaluating materials and methods of dental treatment and also articles concerned with dental education.
Dentists can learn about new concepts, clinical developments, clinical opinions, treatments and the latest advances in techniques and instrumentation that helps them keep pace with rapid changes in this field and provide them with a knowledge transfer platform for rapid publication of reports and articles through an international journal.
This journal encourages researches concerning improvement of dental health through the latest technologies as tissue engineering, nanotechnology, laser application and implantology.
The journal is interested in inter- disciplinary articles within the dental field, and group work with other disciplines as medicine, physics and chemistry. The journal also aims to provide clinicians, scientists and students of dentistry with a knowledge transfer platform for rapid publication of reports and articles through an international journal.Subject areas include, but are not limited to the following fields: •
Periodontics
Orthodontics
Endodontics
Oral medicine
Dental Radiology
Oral surgery
Dental materials
Oral sciences
Pedodontics
Oral biology
Prosthodontics
Dental implants
Crown and Bridge
Oral Pathology and Microbiology
Community dental health
Oral hygiene
Cosmetic dentistry
Dental educationFuture University In Egyp
Selective determination of tolterodine tartrate in presence of its oxi- dative degradation product
No full textFour stability-indicating methods were developed for determination of tolterodine tartrate in the presence of its oxidative degradation product (the metabolite). The degradation product was prepared via oxidation with hydrogen peroxide. The degradation product was characterized and struc- turally elucidated. The first method was the first derivative 1D by measur- ing the peak amplitude at 292nm. The second method was a second de- rivative by measuring the peak amplitude at 236, 287, 296 nm. The third method was a high performance liquid chromatographic using a reversed phase column and a mobile phase of phosphate buffer: methanol: triethyl amine (60: 40; 0.2 by volume). The forth method was a thin layer chroma- tography coupled with densitometric detection.Selective quantification of tolterodine in pure form, pharmaceutical formu- lation and/or in the presence of its degradant was demonstrated. The indi- cation of stability was done under condition likely to be expected at normal storage condition. 2016 Trade Science Inc. - INDI
Future Business Journal Volume 2, Issue 2
No full textArticles : Exchange rate volatility and private consumption in Sub-Saharan African countries: A system-GMM dynamic panel analysis -- Does relational leadership generate organizational social capital? A case of exploring the effect of relational leadership on organizational social capital in China -- Promoting thriving at work and waning turnover intention: A relational perspective --
Intrinsic value estimates and its accuracy: Evidence from Indian manufacturing industry -- Economic value added or earnings: What explains market value in Indian firms?The Future Business Journal is a blind peer-reviewed journal that will be published bi-annually by Future University in Egypt. It is dedicated to increasing the depth of the subject across disciplines with the ultimate aim of expanding knowledge of the subject.
The Future Business Journal (FBJ) publishes conceptual and empirical research papers spanning all the major research fields in Management, Marketing, Accounting, Micro Economics, Management Information Systems, and Finance. The Journal's emphasis is on theoretical developments and their implementation, empirical, applied, and policy-oriented research in Management, Marketing, Accounting, Micro-Economics Management Information System and Finance. The Journal's purpose is to improve communications between, and within, the academic and other research communities and policymakers and operational decision makers. Future Business Journal fills a unique niche among business publications of its type by publishing articles that strike a balance between the practical and the academic fields, recognizing the intricate relationships between many areas of business activities.Future University In Egyp
Atlas of Oral Diseases : A Guide for Daily Practice / Isaäc van der Waal.
No full textdental bookfair2016xv, 183 p. :This atlas is designed to assist all who are involved in diagnosing and treating oral diseases. Individual chapters focus on lesions and disorders of the oral mucosa, soft tissues (including the minor salivary glands), lips, tongue, gingiva, palate, and jaw bones (odontogenic and non-odontogenic lesions). In addition to the more common diseases, less frequent disorders are also covered, some of which have been recognized only in recent years. Throughout, the approach is practice oriented, with concise text and an abundance of high-quality clinical, radiographic, and, where appropriate, histopathologic images. The combined training of the author in oral surgery and oral pathology means that he has exceptional expertise in both the diagnosis and the treatment of oral diseases. His detailed knowledge and experience are fully reflected in the Atlas of Oral Diseases, which will be very helpful for dental and medical professionals in their daily practice
Autonomous Vehicle
No full textSelf-drive, and assist in lane changing
Obstacle avoidance and crash prevention
Localization and road mapping of 3D-Environment.
Route planning and way-point of path plannin