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EFFECT OF L-TRYPTOPHAN ON SPERM QUALITY OF TIGRIS SCRAPER (Capoeta umbla) (Pisces: Cyprinidae) AFTER CRYOPRESERVATION
No full textBACKGROUND:
The aromatic amino acid L-tryptophan is a versatile molecule and needed for the biosynthesis of proteins. Due to metabolic functions, it has been widely used in research and clinical trials.
OBJECTIVE:
The present study aimed to test the usefulness of L-tryptophan as an extender of Tigris scraper (Capoeta umbla) sperm during cryopreservation.
MATERIALS AND METHODS:
Semen samples were diluted with a ratio of 1:3 (sperm: extender) and base extenders were supplemented with L-tryptophan (0 mM, 0.5 mM, 1 mM, 2 mM and 4 mM). Sperm motility and duration of post-thawed samples were assessed after the cryopreservation process.
RESULTS:
Our results revealed that motility rate and duration of sperm increased when the cryomedia was supplemented with L-tryptophan. On the other hand, an increase in the concentration of L-tryptophan in the extender caused a significant decrease (p<0.05) in the motility rate of Tigris scraper (C. umbla) sperm. The ideal results were obtained at 1 mM L-tryptophan; however, no motile sperm were shown in any samples when a concentration of 2 mM was used.
CONCLUSION:
The current study shows the importance of conducting further studies related to long-term storage and reproduction management
The usability of oxidative stress and detoxification biomarkers in Gammarus pulex for ecological risk assessment of textile dye methyl orange
No full textThe present study was undertaken to determine the toxicity of the methyl orange by using the changes of some antioxidant and detoxification enzyme activities in Gammarus pulex. Lethal Concentration (LC) value of Methyl Orange (MO) was determined. Three sublethal doses of MO (1/4; 1/8 and 1/16 of LC value) were exposed to G. pulex for 24 and 96 h. Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GSH-PX), Cytochrome p450 (CYP1A1), Glutathione S-transferase (GST) activities as well as Glutathione (GSH) and Malondialdehyde (MDA) levels were determined by using The enzyme-linked immunosorbent assay (ELISA) kit. The CAT and CYP1A1 activities were decreased in all the groups exposed to different doses of MO. GST activity and GSH, MDA levels were increased all the groups exposed to different doses of MO. The GSH-PX activities were changed in all the groups. MO affected SOD activity at different levels and in different concentrations. In our study, it has been found that exposure duration didn’t significantly affect the biochemical biomarkers except for GST and GSH. In conclusion, alterations in antioxidant and detoxification enzymes and lipid peroxidation may potentially be used as sensitive biomarkers for risk assessment of dyes in the environment and may contribute to the establishment of discharge regulations
Effect of L-tryptophan on sperm quality of tigris scraper (capoeta umbla) (pisces: cyprinidae) after cryopreservation
No full textBACKGROUND: The aromatic amino acid L-tryptophan is a versatile molecule and needed for the biosynthesis of proteins. Due to metabolic functions, it has been widely used in research and clinical trials. OBJECTIVE: The present study aimed to test the usefulness of L-tryptophan as an extender of Tigris scraper (Capoeta umbla) sperm during cryopreservation. MATERIALS AND METHODS: Semen samples were diluted with a ratio of 1:3 (sperm: extender) and base extenders were supplemented with L-tryptophan (0 mM, 0.5 mM, 1 mM, 2 mM and 4 mM). Sperm motility and duration of post-thawed samples were assessed after the cryopreservation process. RESULTS: Our results revealed that motility rate and duration of sperm increased when the cryomedia was supplemented with L-tryptophan. On the other hand, an increase in the concentration of L-tryptophan in the extender caused a significant decrease (p<0.05) in the motility rate of Tigris scraper (C. umbla) sperm. The ideal results were obtained at 1 mM L-tryptophan; however, no motile sperm were shown in any samples when a concentration of 2 mM was used. CONCLUSION: The current study shows the importance of conducting further studies related to long-term storage and reproduction managemen
Determination of financal literacy knowledge level : case of public emploees in the Tunceli province
No full textThe recent market risk with globalization has drawn attention to the concept of financial literacy, which can be expressed as the complexity of financial products and services, the ability to use financial information and the ability to use the acquired financial information, given the diversification of financial products and financial decisions. Financial literacy; In recent years, it is a concept that is frequently researched in the literature in order to increase the savings of individuals, to manage the financial crises better and to make healthy financial decisions.
In this study, it is aimed to determine whether financial literacy levels of public employees in Tunceli province and financial literacy level of employees differ according to demographic characteristics. The data used in the study was obtained by means of the questionnaire formed in the context of literature.The data were analyzed in SPSS 20.0 Social Sciences Statistical Analysis program. The questionnaire applied to public employees consists of 23 items that measure the level of financial literacy and statements defining demographic information. As a result of the study, it was determined that there was a significant difference between the demographic characteristics of public employees and financial literacy knowledge levels
Application of Response Surface Methodological Approach to Optimize Removal of Cr Ions from Industrial Wastewater
No full textIn the present study, the adsorption of chromium (Cr) onto clay using response surface methodology (RSM) was investigated as an efficient approach for examining predictive model building and optimization. To optimize experimental variables and predictive regression models, statistical design models were carried out. A five-level four-factor central compozite design (CCD) combined with RSM was performed with synthetic aqueous solutions on natural clay. The experimental variables included pH and agitation speed and were selected for optimization. The Cr ions ideal removal conditions were obtained using pH 5.0, contact time 23.0 minutes, adsorbent dosage of 69.4 mg, and agitation speed of 135 rpm. Chromium
removal efficiency was found at 23.16 mg g-1. The independent variables significance and their interactions were verified by means of the analysis of variance (ANOVA). These results were justified by the relatively high correlation coefficients (R2 =0.9902) of the statistical prediction. Clay and Cr loaded clay were characterized by X-ray diffractometer (XRD) and energy dispersive spectroscopy (EDS).
The proposed adsorption process efficiently removes the Cr ions present in various aqueous media
and was also applied to various industrial wastewaters
Research of thermal performance investigation of stator winding solder region in hydroelectric power plants
No full textHydroelectric power plants are the most widely used renewable energy sources all over the world. In these plants, many studies have been conducted in order to produce energy continuously and efficiently. Among these studies, predictive maintenance methods, efforts to increase power by improving cooling conditions, and improving efficiency by improving the turbine wheel. In this paper, soldering at the junctions of the stator windings in the central generators has been investigated. Traditional solder (Cu-Zn) materials and silver alloy solders (Ag-Cu-Zn) have been investigated in terms of thermal and metallographic. Silver alloy soldering has been found to have lower electrical conductivity than conventional soldering. The winding temperatures measured on the generating generator show that silver alloy soldering is more efficient. Reducing the stator winding temperature in hydroelectric power plants allows more energy production and also reduces costs by preventing winding failures
Öğretmenlik ve Dijital Emek
No full textGünümüz bilgi toplumlarında maddi karşılığı olmayan emek, dijital dünyanın önemli başlıklarından
biri haline gelmiştir. Dijital emek, çevrimiçi medya için bilgi ve içerik oluşturulurken ödenmemiş
emeğin sömürülmesi olarak tanımlanmaktadır. Dijital emek diğer sektörlerde olduğu gibi eğitim
sektöründe de sıkça görünür olmuştur. Öyle ki öğretmenler günümüz teknolojileri sayesinde ders
sunularını kendi hazırlamak yerine internetten hazırlanmış olan hazır sunuları herhangi bir ücret
ödemeden indirip ders materyali olarak kullanabilir olmuştur. Bu araştırma ile öğretmenlerin dijital
emek hassasiyetleri belirlenmeye çalışılmıştır. Araştırma nitel araştırma paradigması doğrultusunda
şekillendirilmiştir. Araştırma verileri yarı yapılandırılmış görüşme formu ile toplanmış, veriler içerik
analizi yöntemi ile analiz edilmiştir. Yapılan analizler sonucunda elde edilen bulgulara ve bu bulgular
doğrultusunda ortaya çıkan sonuçlara, tartışmaya ve önerilere ayrıca yer verilecektir
Identification of optimum working conditions in hydroelectric power plants for cavitation
No full textTurbine faults in hydroelectric power plants not only reduce the operation time of the plant, but also cause a serious loss of income. One of the most important turbine faults is the turbine wheel wear caused by the cavitation and accordingly the other machine equipment faults which cause excessive vibration. In this study, cavitation intensity that the turbines have been exposed in operating conditions has been investigated. As a result of the calculations, optimum working conditions of the units have been determined with graphs obtained in various operating conditions. The obtained data can be easily applied to all hydroelectric power plants exposed to cavitation, and safe operating conditions can be determined
Uzunçayır baraj gölü fitoplankton tür kompozisyonu ve fiziko-kimyasal parametrelerin belirlenmesi
No full textUzunçayır Baraj Gölü fitoplankton dağılımı ve bazı fiziko-kimyasal parametreleri, Nisan 2017 - Mart 2018 tarihleri arasında araştırma alanı olarak seçilen Uzunçayır Baraj Gölünde (Tunceli) belirlenmiş olan yedi istasyon da incelenmiştir. Araştırma süresince Uzunçayır Baraj Göl’ü yüzey sularında yıl boyu alınan parametrelerin ortalama değerleri; sıcaklık için 13,2 ºC, pH 8,5 çözünmüş oksijen miktarı 11.1 mg/l olarak, toplam azot miktarı 1.13 olarak, toplam fosfor miktarı 0.007 mg P/L, olarak elektriksel iletkenlik 304 ms/cm, klorofil-a 1,1 mg /L ve seki disk derinliği 2,3 m olarak hesaplanmıştır.
Uzunçayır Baraj Gölü’nde Çalışma süresince; tüm istasyonlarda fitoplankton olarak; Bacillariophyta (72 takson), Charophyta (6 takson), Chlorophyta (18 takson), Chrysophyta (3 takson), Cyanophyta (15 takson), Dinophyta (13 takson) ve Euglonozoa (8) olmak üzere toplam 135 takson kaydedilmiştir. Çalışma sırasında örnekler yüzeyden plankton kepçesiyle alınmış, fitoplankton topluluklarının aylık durumları izlenmişti
Age and some growth parameters of Squalius cephalus (Linnaeus,1758) inhabiting Karasu River (East Anatolia, Turkey)
No full textThe present study was carried out concerns a research of the age determination and some growth parameters of chub, Squalius cephalus (Linnaeus, 1758) living in 14 different station from Karasu River (East Anatolia, Turkey). For this purpose, a total of 196 fish specimen (100 females and 96 males) of S.cephalus were captured between 2014 and 2016 by electroshocker. Female/male ratio was 1/0.96. Minimum maximum length and weight of captured fishes were determined as 7.6-23.9 cm and 4.8-198.2 g, respectively. The age groups were determined between 0 and 6. The parameters of von Bertalanffy growth function estimated as L∞=31.98 cm, k=0.136 year-1, t 0 =-1.40 year for all individuals. The growth performance index (Ф›) value was computed as 2.143. The length and weight relationships W=0.0060L3.27 (R2 =0.98) for all individuals. Significant statistical differences in condition factors between age groups and sexes were not found (P 0.05, t-test)