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An inflammatory bowel disease-associated SNP increases local thyroglobulin expression to develop inflammation in miniature dachshunds
Get PDFjournal articleInflammatory colorectal polyp (ICRP) in miniature dachshunds (MDs) is a chronic inflammatory bowel disease (IBD) characterized by granulomatous inflammation that consists of neutrophil infiltration and goblet cell hyperplasia in the colon. Recently, we identified five MD-associated single-nucleotide polymorphisms (SNPs), namely PLG, TCOF1, TG, COL9A2, and COL4A4, by whole-exome sequencing. Here, we investigated whether TG c.4567C>T (p.R1523W) is associated with the ICRP pathology. We found that the frequency of the T/T SNP risk allele was significantly increased in MDs with ICRP. In vitro experiments showed that TG expression in non-immune cells was increased by inducing the IL-6 amplifier with IL-6 and TNF-α. On the other hand, a deficiency of TG suppressed the IL-6 amplifier. Moreover, recombinant TG treatment enhanced the activation of the IL-6 amplifier, suggesting that TG is both a positive regulator and a target of the IL-6 amplifier. We also found that TG expression together with two NF-κB targets, IL6 and CCL2, was increased in colon samples isolated from MDs with the T/T risk allele compared to those with the C/C non-risk allele, but serum TG was not increased. Cumulatively, these results suggest that the T/T SNP is an expression quantitative trait locus (eQTL) of TG mRNA in the colon, and local TG expression triggered by this SNP increases the risk of ICRP in MDs via the IL-6 amplifier. Therefore, TG c.4567C>T is a diagnostic target for ICRP in MDs, and TG-mediated IL-6 amplifier activation in the colon is a possible therapeutic target for ICRP.journal articl
Nonsuppurative Encephalitis with Detection of a Neurotropic Astrovirus Gene in a Cow
Get PDFjournal article群馬県内の酪農場において，非化膿性脳炎を呈した牛の大脳からアストロウイルス（AstV）の特異遺伝子断片が検出された．当該牛は横臥し泌乳量減少，食欲不振となり，予後不良と診断された．剖検では，著変は認めず，病理組織検査では，大脳，間脳，中脳，橋及び延髄に囲管性細胞浸潤，グリア集簇が認められ，それらの変化は脳幹でより重度であった．病原検索では，大脳及び主要臓器から有意な細菌は分離されず，他のウイルスは不検出であった．検出されたAstV遺伝子断片の塩基配列は，国内初発例の神経指向性AstVの非構造蛋白質領域と95％一致した．本症例の脳炎にAstVが関与した可能性があり，国内2例目の報告であると考えられる．本研究により，国内の複数の地域における神経指向性AstVの存在が示唆され，国内にAstVがまん延している可能性が推察された．At a dairy farm in Gunma Prefecture, Japan, a specific gene fragment of astrovirus (AstV) was detected in the cerebrum of a cow with nonsuppurative encephalitis. The cow laid down, lactation decreased, lost its appetite, and the prognosis was poor. No gross abnormalities were detected during the necropsy. Histopathological examination of the cerebrum, diencephalon, midbrain, pons, and medulla oblongata revealed multiple vessels cuffed by numerous lymphocytes and glial nodules, which were more severe in the brain stem. No pathogenic bacteria were isolated from the cerebrum and other organs, and no other viruses were detected. The nucleotide sequence of the detected AstV gene fragment had a 95% coincidence with the non-structural protein region of neurotropic AstV reported in the first confirmed case in Japan. AstV may have been involved in this case of encephalitis, which is considered to be the second reported case in Japan. This study suggests the existence of neurotropic AstV in multiple regions of Japan and infers the possibility that AstV is prevalent in Japan.journal articl
The Effect of Free Access to the Indoor Room in Hot Summer on the Behavior of Red pandas
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Consumer Preferences for Sustainable Dairy Products : Effects of GHG Reduction and Feed Self-sufficiency on Milk Choice
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Effect of media composition on yield and spear quality of white asparagus in forcing culture using bag
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