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Monitoring of Vegetation Disturbance around Protected Areas in Central Tanzania Using Landsat Time-Series Data
Get PDFArticleUnderstanding vegetation disturbance around protected areas (PAs) is critical as it significantly affects the sustainable conservation of wildlife. However, there is a lack of analyses of consistent long-term data on vegetation disturbance. In this study, the LandTrendr algorithm and Google Earth Engine were used to access satellite data and explore the vegetation dynamics history across the Ruaha–Rungwa landscape, Tanzania. We characterized vegetation disturbance patterns and change attributes, including disturbance occurrence trends, rate, and severity, by using each pixel’s normalized burn ratio index time series. Between 2000 and 2019, 36% of the vegetation was significantly disturbed by anthropogenic activities. The results of this study show that the disturbance trends, severity, and patterns are highly variable and strongly depend on the management approaches implemented in the heterogeneous landscape: Ruaha National Park (RNP), Rungwa–Kizigo–Muhesi Game Reserves (RKMGR), and the surrounding zones. The disturbance rates and severity were pronounced and increased toward the edges of the western RKMGR. However, the disturbance in the areas surrounding the RNP was lower. The characterization of the vegetation disturbance over time provides spatial information that is necessary for policy makers, managers, and conservationists to understand the ongoing long-term changes in large PAs.journal articl
Role of insulin, insulin sensitivity, and abomasal functions monitors in evaluation of the therapeutic regimen in ketotic dairy cattle using combination therapy with referring to milk yield rates
Get PDFArticleBackground: Ketosis is one of the most critical metabolic disorders that occur in dairy cows after parturition due to negative energy balance around calving. Aim: The study evaluated a specific therapeutic regimen of ketosis in Holstein dairy cattle by using the combination therapy including hormones, corticosteroids, propylene glycol, and vitamin B12 as well as the use of milk yield rates, insulin, insulin sensitivity, and abomasal functions monitors as diagnostic biomarkers for the recovery of ketotic cows either pre-therapy (0 days) or post-therapy (7 and 14 days). Methods: This study was conducted on ketotic cattle (n = 20) belonged to different dairy farms in Cairo and Giza governorates, Egypt. The diseased cows were undergoing clinical and biochemical investigations for the estimation of serum insulin. Quantitative Insulin Sensitivity Check Index (RQUICKI) and abomasal functions monitor mainly serum levels of gastrin, pepsinogen, and chloride. Results: The milk production rates, cost: benefit analysis ratio, and benefit of the dairy farm in ketotic animals were significantly increased post-treatment. An improvement of insulin sensitivity was stated as serum insulin, and RQUICKI were remarkably increased in post-therapeutic ketotic cows. Monitors of the abomasal function revealed abomasal functions improvement through the significant elevation of blood gastrin and a substantial reduction in serum pepsinogen due to treatment. Conclusion: The study revealed high efficacy of the applied therapeutic strategy regime. It led to a high recovery rate and a very low relapse rate for ketosis. An improvement in milk yield rates, insulin sensitivity, and abomasal function monitors was reported. Hypoinsulinaemia was still reported, however, serum insulin was improved.journal articl
Irisin/FNDC5: A participant in camel metabolism
Get PDFArticleThe quantification, localization, production, function, and regulation of irisin/FNDC5 in camel species have not been previously studied. The objective of this study was to detect the irisin content in Arabian camel blood and tissues and study the gene expression of FNDC5 and PGC-1α in camel skeletal muscles and white adipose tissue depots under basal conditions. To monitor if exercise influences blood and tissue irisin protein levels as well as FNDC5 and PGC-1α gene expression levels, we analyzed irisin concentrations in the serum, skeletal muscles (soleus and gastrocnemius), and white adipose tissues (hump, subcutaneous, visceral, epididymal, and perirenal) in both control (n = 6) and exercised group (n = 6) using ELISA and determined the cellular localization of irisin/FNDC5 and the mRNA levels of FNDC5 and PGC-1α in skeletal muscles and adipose tissues via immunohistochemistry and real-time PCR, respectively. The possible regulatory roles of exercise on some hormones and metabolites as well as the detection of links between serum irisin and other circulating hormones (insulin, leptin, and cortisol) and metabolites (glucose, free fatty acids, triglycerides, and ATP) were explored for the first time in camels. Our results indicated that exercise induces tissue-specific regulation of the camel irisin, FNDC5, and PGC-1α levels, which subsequently regulates the circulating irisin level. Significant associations were detected between the levels of irisin/FNDC5/PGC-1α in camels and the metabolic and hormonal responses to exercise. Our study suggested that irisin regulates, or is regulated by, glucose, FFA, insulin, leptin, and cortisol in camels. The novel results of the present study will serve as baseline data for camels.journal articl
Evaluation of nosocomial infections through contact patterns in a small animal hospital using social network analysis and genotyping techniques
Get PDFArticleNosocomial infections or hospital-acquired infections (HAIs) are common health problems affecting patients in human and animal hospitals. Herein, we hypothesised that HAIs could be spread through human and animal movement, contact with veterinary medical supplies, equipment, or instruments. We used a combination of social network analysis and genotyping techniques to find key players (or key nodes) and spread patterns using Escherichia coli as a marker. This study was implemented in the critical care unit, outpatient department, operation room, and ward of a small animal hospital. We conducted an observational study used for key player determination (or key node identification), then observed the selected key nodes twice with a one-month interval. Next, surface swabs of key nodes and their connecting nodes were analysed using bacterial identification, matrix-assisted laser desorption/ionisation-time of flight mass spectrometry, and pulsed-field gel electrophoresis. Altogether, our results showed that veterinarians were key players in this contact network in all departments. We found two predominant similarity clusters; dendrogram results suggested E. coli isolates from different time points and places to be closely related, providing evidence of HAI circulation within and across hospital departments. This study could aid in limiting the spread of HAIs in veterinary and human hospitals.journal articl
美唄市と根室市におけるツルコケモモ（Vaccinium oxycoccos L.）の葉緑体ゲノムの遺伝変異
Get PDFArticle本実験は日本におけるツルコケモモの塩基情報に関する基礎的知見を得るため，北海道に自生するツルコケモモの葉緑体ゲノム5領域の塩基配列を調べた。その結果，塩基配列を決定した領域のうち，rbc L領域とtrn L（UAA）3' exon - trn F（GAA）領域で遺伝変異が認められ，3種類のハプロタイプの存在が明らかになった。また，これらの遺伝変異には地域的な違いが見られた。journal articl
