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    Toxoplasma gondii in northeastern Portugal: a narrative review of a neglected zoonotic infection in a one health context

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    Toxoplasmosis is a zoonosis caused by Toxoplasma gondii, a protozoan parasite that infects warm-blooded animals, including humans1. Despite its global distribution and considerable impact on public health, animal production, and the environment, it remains overlooked, exemplifying a One Health issue2,3. Transmission occurs mainly through ingestion of infective stages: sporulated oocysts, via contaminated water, soil, or food, tissue cysts in undercooked meat, or tachyzoites, through transplacental transmission or unpasteurized dairy products1,4. Though often asymptomatic, toxoplasmosis can cause severe outcomes in immunocompromised individuals and during pregnancy. Small ruminants are particularly susceptible, with infection linked to reproductive losses and zoonotic transmission via meat5. In Portugal, T. gondii infection, and toxoplasmosis, remains underreported and poorly studied. The Northeastern region (Trás-os- Montes) is especially relevant due to the high density of small ruminant farming and close human, animal and environment interactions6. A narrative review of the literature on the epidemiological status of T. gondii infection in Northeast Portugal was carried out. Sources included PubMed, Scopus, Web of Science, Google Scholar, and institutional reports (EFSA, ECDC, INE). The EFSA/ECDC/20237 report a 4.2% rise in congenital toxoplasmosis and a 29% positivity in small ruminants, the highest in five years. In Portugal, according to the only national survey (1979–1980)8, seroprevalence was 47%, with the North presenting the highest percentage (51%). Subsequent studies revealed regional fluctuations8–12 and more recently, a 72.8% value was reported amongst workers with occupational exposure13. In the Northeastern region (2004–2010)14–17, active circulation of T. gondii was demonstrated in various animal species including wild and livestock, particularly small ruminants, as well as in women of childbearing age. The detection of genotype II, associated with moderate virulence, in meat intended for consumption reinforces the zoonotic risk18. These findings highlight the urgent need for updated and integrated epidemiological studies, supported by a One Health approach

    A review of best methods for 3D scanning and modeling of terrain and buildings using drones and photogrammetry

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    Recent advancements in drone technology and photogrammetry have revolutionized the field of 3D terrain and building modelling. Drones equipped with photogrammetric software provide efficient, accurate, and cost-effective solutions for capturing high-resolution 3D models of both natural and urban environments. This literature review consolidates research on best practices for terrain and building scanning using drones and photogrammetry, focusing on methodologies, integration of technologies, challenges, and future directions. Additionally, the review explores applications in urban planning, environmental monitoring, disaster management, and heritage preservation

    Digital innovation in tourism: exploring the potential of chatbots

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    In the last decade there has been a substantial increase in references to Artificial Intelligence (AI). AI has entered people’s lives, without them often realizing it. AI is increasingly present in our lives and in our daily lives, from our homes, transport, travel, industry, etc. It is inseparable to talk about AI without talking about chatbots. Therefore, the main objective of this study is to study the potential of chatbots in the tourism industry. For this purpose, a questionnaire was carried out among Higher Education students in Portugal, as it is understood that it is a good universe of study, since these technologies are still more associated with young audiences. On the other hand, also as a result of programs such as Erasmus, these young people travel more and are more aware of the potential of chatbots in tourism. The results of the questionnaire are presented in light of the literature and the responses collected, as well as the limitations of the present study. Future work that may mitigate the limitations of this research work are also presented.e authors are grateful to the UNIAG, R&D unit fimded by the FCT— Portuguese Foundation for the Development of Science and Technology, Ministry of Science, Technology and Higher Education. "Project Code Reference: UIDB/04752/2020 e UIDP/04752/2020"

    Habilidade empática do cuidador formal de uma pessoa idosa: um estudo exploratório

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    Este estudo de natureza quantitativa, descritiva e exploratória, tem como objetivo analisar a habilidade empática de auxiliares de ação direta de pessoas idosas em função das suas características sociodemográficas e profissionais. Para a recolha de dados foi utiizado um questionário composto por itens sociodemográficos e profissionais, além da versão validada para a população portuguesa do Índice de Reatividade Interpessoal. Colaboraram neste estudo 177 profissionais, na sua maioria mulheres (96,61%), casadas ou em união de facto (60,45%), com educação secundária completa (56,60%) e uma média etária de 46,54 anos. O tempo médio de experiência profissional foi de 10,15 anos. Os resultados indicaram que os níveis médios de empatia foram superiores à média teórica da escala, com os seguintes fatores em ordem decrescente: Preocupação Empática, Tomada de Perspetiva, Fantasia e Desconforto Pessoal. Importa salientar que estes valores não se diferenciaram em função da idade, do estado civil e do nível de escolaridade dos participantes. Constatou-se, porém, que os cuidadores com mais experiência e maior satisfação profissional apresentaram níveis mais elevados de empatia afetiva. Além disso, aqueles que assinalaram maior satisfação profissional evidenciaram maiores níveis de empatia cognitiva e afetiva. Infere-se dos dados obtidos a necessidade da continuidade relacional e institucional por parte dos cuidadores, e do investimento em programas que fortaleçam o desenvolvimento de habilidades empáticas nestes profissionais, o que poderá ter um impacto significativo na melhoria dos serviços prestados e na qualidade de vida das pessoas idosas.Este trabalho foi apoiado pela FCT - Fundação para a Ciência e Tecnologia no âmbito do Centro de Investigação em Educação Básica com a referência UIDB/05777/2020 (hwps://doi.org/10.54499/UIDB/05777/2020).info:eu-repo/semantics/publishedVersio

    Controlling Listeria monocytogenes contamination in fresh goat milk cheeses: Dynamic modelling during storage

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    Predictive models applied for artisanal fresh goat cheeses are invaluable tools for rapid decision-making in quality and safety management. Listeria monocytogenes is a common foodborne pathogen associated with recent safety alerts in ready-to-eat (RTE) products in Andalucia. Managing artisanal production variables, such as storage conditions and final product features (pH or water activity [aw]) might be crucial for controlling L. monocytogenes in these traditional products. This study evaluated the time-temperature dependence of L. monocytogenes during shelf-life of fresh goat milk cheese artisanally produced in Andalucía. The effect of storage temperature (4–25 ◦C) over 5–20 days was tested in lab-scale fresh goat milk cheeses initially inoculated with a three-strain cocktail of the pathogen (ca. 2–3 log cfu/g). The Huang (model A) and Baranyi (model B) models coupled with Ratkowsky were fitted to the collected growth data to describe the relationship between L. monocytogenes concentration, growth rate (μmax, log cfu/d), storage temperature, and time. The results highlighted the higher variability of growth data at 4 ◦C, the linear relationship between μmax and temperature, the inverse correlation between ymax (exceeding 5 log cfu/g at all the temperatures) and ΔY (with a maximum of 4.386 and minimum of 2.461, at 4 and 25 ◦C, respectively) and a faster decrease of pH at higher temperatures. Model validity, assessed through goodness-of-fit and both internal and external validations (under both isothermal temperatures and a dynamic temperature workflow simulating real production scenario), revealed good model adjustment to L. monocytogenes growth in artisanal commercial cheeses, after determining a correction factor of 1.806 and 1.856, for models A and B, respectively. Predictions indicated that an initially contaminated artisanal fresh goat milk cheese would not meet regulatory limits after 4 days under refrigeration conditions (4 ◦C), aerobic conditions, or opened packaging. These results highlight the potential public health risk of artisanal fresh goat milk cheeses related to L. monocytogenes transmission if manufacturing and storage conditions are not well controlled, emphasizing the need for stringent microbial control efforts and mitigation measures.The authors are grateful to EU PRIMA program and the International Joint Programming (Project Reference PCI2019-103453) R&D Projects 2019 from the Spanish Ministry of Science and Innovation (Plan Estatal de Investigación Científica y Técnica y de Innovación 2017–2020: State R&D Program Oriented to the Challenges of the Society) for funding the ArtiSaneFood project (PRIMA/0001/2018)

    Combined action of dietary-based approaches and therapeutic agents on cholesterol metabolism and main related diseases

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    Dyslipidaemia is among the major causes of severe diseases and, despite being wellestablished, the hypocholesterolaemic therapies still face significant concerns about potential side effects (such as myopathy, myalgia, liver injury digestive problems, or mental fuzziness in some people taking statins), interaction with other drugs or specific foods. Accordingly, this review describes the latest developments in the most effective therapies to control and regulate dyslipidaemia. Scope and approach: Herein, the metabolic dynamics of cholesterol and their integration with the current therapies: statins, bile acid sequestrants, fibrates, niacin, proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors, reconstituted high-density lipoprotein (rHDL), or anti-inflammatory and immunemodulating therapies), were compared focusing their effectiveness, patients’ adhesion and typical side-effects. Likewise, the interaction of these therapies with recommended dietary habits, focusing functional foods and nutraceuticals uptake were also considered. Key findings and conclusions: Since none of the current therapeutic alternatives represent an ideal solution (mainly due to side-effects or patients’ tolerance), the potential adjuvant action of selected diets (and other healthy habits) was proposed as a way to improve the cholesterol-lowering effectiveness, while reducing the adverse effects caused by dose-increase or continuous uptake of alternating therapeutic agents. In general, the relevance of well-adapted diets must be acknowledged and their potential effects must be exhorted among patients, who need to be aware of the associated multifactorial advantages.This work was supported by UID/QUI/50006/2019 with funding from FCT/MCTES through national funds. J.C.M. Barreira acknowledges Fundação para a Ciência e a Tecnologia (FCT) for his contract (CEECIND/04479/2017); C.S.G.P. Pereira thanks FCT for her PhD grant (2021.09490.BD).info:eu-repo/semantics/publishedVersio

    Production of ethanolic biodiesel from waste cooking oils and purification through adsorption using olive pits based materials

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    Mestrado de dupla diplomação com a UTFPR - Universidade Tecnológica Federal do Paran

    Stock market effects of corporate malpractices and misconduct: evidence from the short-seller Hindenburg

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    This study used event study methodology to examine the impact Hindenburg Research shortseller reports on targeted firms. The results show negative abnormal returns in firms when those reports reveal bad news about malpractices and misconduct. Our results show a higher negative stock market reaction to the Hindenburg reports when target firms are small, have higher leverage, higher Tobin’s Q, and corporate malpractice involves financial fraud. Our findings evidence that adverse information disclosed in the Hindenburg report led to a “torpedo effect”, resulted in sharp, immediate, and persistent share price drops.This paper is financed by Portuguese national funds through FCT – Fundação para a Ciência e a Tecnologia, I.P., projects numbers UIDB/00685/2020 (António Martins) and FCT/MCTES (PIDDAC): UNIAG, UIDB/04752/2020 and UIDP/04752/2020 (Nuno Moutinho).info:eu-repo/semantics/publishedVersio

    Preparação e caracterização de adsorventes obtidos de caroço de azeitona para remoção de estrogênios

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    Mestrado de dupla diplomação com a UTFPR - Universidade Tecnológica Federal do Paran

    Variabilidade temporal e espacial das precipitações e suscetibilidade à seca no distrito de Bragança - Portugal

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    Grande parte do nordeste de Portugal é composta por zonas áridas, caracterizadas por uma suscetibilidade de moderada a severa à desertificação e à seca. Foi realizada uma análise de tendências, tanto individual quanto combinada, da precipitação e do índice de seca SPI em vinte estações meteorológicas no Distrito de Bragança, em Portugal, abrangendo um período de quarenta anos (1957-1997). A análise revelou que, ao longo do período de estudo, à medida que a escala temporal do SPI aumentou (de 1 para 12 meses), houve um aumento na frequência de meses secos, bem como na ocorrência de meses com secas severas e extremas. Os resultados indicam uma redução gradual ao longo da escala temporal, dos meses classificados como ‘’Próximo ao normal’’ na classificação de SPI, passando de 69% no SPI 1 para 65,5% no SPI 12. Em contrapartida, a percentagem de meses de secas aumentou gradualmente, com valores passando de 14,3% dos meses de seca no SPI 1 a 17% no SPI 12. A percentagem de meses de ‘’seca severa’’ e ‘’seca extrema’’ seguiram o mesmo padrão de aumento dos meses secos, aumentando conforme a escala de análise de SPI 1 a SPI 12. No geral, as tendências identificadas para precipitação e seca sugerem uma intensificação gradual na vulnerabilidade à desertificação na região, embora sem um padrão de variação uniforme na região ao longo das 4 décadas estudadas.Much of northeastern Portugal is arid, characterized by moderate to severe susceptibility to desertification and drought. An individual and combined trend analysis of precipitation and the SPI drought index was performed at twenty meteorological stations in the Bragança District of Portugal, covering a forty-year period (1957–1997). The analysis revealed that, over the study period, as the SPI time scale increased (from 1 to 12 months), there was an increase in the frequency of dry months, as well as in the occurrence of months with severe and extreme droughts. The results indicate a gradual reduction over the time scale of months classified as ‘’Near normal’’ in the SPI classification, going from 69% in SPI 1 to 65.5% in SPI 12. In contrast, the percentage of drought months increased gradually, with values going from 14.3% of drought months in SPI 1 to 17% in SPI 12. The percentage of months of ‘’severe drought’’ and ‘’extreme drought’’ followed the same pattern of increase as dry months, increasing as the scale of analysis from SPI 1 to SPI 12. Overall, the trends identified for precipitation and drought suggest a gradual intensification in vulnerability to desertification in the region, althou

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