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The “Bone Block Technique” : reconstruction of bone defects caused by osteomyelitis using corticocancellous bone blocks from the iliac crest and the induced membrane technique
No full textBackground: The Induced Membrane Technique (IMT), commonly known as the Masquelet Technique (MT), has shown promising results in the reconstruction of bone defects caused by osteomyelitis. However, it is not a standardized surgical protocol but a treatment concept that has undergone various modifications, often yielding heterogeneous outcomes. Methods: This retrospective, single-center clinical cohort study included 49 patients treated with the Bone Block Technique (BBT) between 2013 and 2019 for bone defects resulting from osteomyelitis. The primary outcomes were time to bone healing, reinfection rate, and time to full weight-bearing. Additionally, infectious disease parameters, surgical site complications (SSCs), and epidemiological data were evaluated. Results: Data from 49 patients (mean age: 51 years, range: 17.6–76.9; 28.6% female) were analyzed, with a mean follow-up of 6.1 years (range: 4–10.5). The average bone defect length was 4.2 cm (range: 2.1–8.4 cm), predominantly involving the lower extremity. Primary bone consolidation was achieved in 93%, and secondary consolidation (requiring additional surgery) in 7%. Revision surgery due to recurrent infection was necessary in 16.6% of cases. The average time to full weight-bearing was 101.3 days. Conclusions: The BBT, as a modified approach based on the original IMT, represents a viable and reproducible option for bone defect reconstruction. When applied in accordance with the principles of the Diamond Concept, this technique facilitates reliable primary consolidation with a low complication rate
Mediterrane Ernährung und Long COVID: Eine Studie zur Untersuchung des Einflusses der Ernährung auf das Post-COVID-Syndrom
No full textLiteraturverzeichnis: Seite 70-7
Soil microbial communities are more disrupted by extreme drought than by gradual climate shifts under different land-use intensities
No full textIntroduction: Extreme events like droughts are expected to increase in frequency due to climate change and will affect ecosystems and their associated key functional components particularly soil microbial communities. Studies simultaneously addressing a range of climate stressors, such as extreme drought events and gradual long-term shifts in precipitation and temperature on soil microbial diversity, community composition and function in agricultural systems are limited.
Methods: Here, we present a data set from a field site in Central Germany comprising two spring growing seasons, one with normal precipitation amount, the other experiencing an extreme drought. Further, the experiment included a climate treatment simulating climate change induced gradual shifts in precipitation and temperature in croplands and grasslands under varying management intensities.
Results and discussion: Our findings demonstrate that the extreme drought had a stronger effect on microbial biomass, functions and community composition than the mild experimental climate treatment mediated by soil moisture differences. The fungal communities were more responsive to the drought than the bacterial community, particularly in croplands, where we observed higher C-cycling enzymatic activities under drought. In contrast, microbial functions in grasslands remained largely unchanged in grasslands under drought, implying lower sensitivity to drought in grassland than cropland systems. However, intensively managed grasslands appeared less stable in community composition and function than extensively managed grasslands, which was also observed in constructed co-occurrence networks. Overall, our results suggest that intensively managed systems are more vulnerable to extreme drought conditions with an increase of fungi with pathogenic potential which may further destabilize soil microbial communities in the future These findings underscore the need to consider multiple stressors such as extreme events and land-use intensity in order to understand the soil microbial response to global change
The role of the ALDH family in predicting prognosis and therapy response in pancreatic cancer
No full textBackground: Pancreatic cancer ranks as the fourth leading cause of cancer-related deaths in the USA. The human aldehyde dehydrogenase (ALDH) family comprises 19 functional members and has been implicated in prognosis and therapy resistance. However, it remains unclear which specific ALDHs are associated with adverse prognoses in pancreatic cancer. Methods: We obtained transcriptomic and clinical data for pancreatic adenocarcinoma (PAAD) from the TCGA, corresponding mutational data, and normal pancreatic tissue transcriptomic data from GTEx. Prognostic analysis was carried out using Kaplan–Meier analysis. KEGG and GO analyses were used for biological signaling pathways, and ESTIMATE algorithms were used for tumor microenvironment (TME) assessment. CIBERSORT algorithm, immune infiltration analysis, and OncoPredict algorithms were employed for predicting chemotherapy sensitivity. Results: Our study identified four of the 19 ALDH genes (ALDH1L1, ALDH3A1, ALDH3B1, ALDH5A1) that were significantly associated with pancreatic cancer prognosis. High expression of ALDH1L1, ALDH3A1, and ALDH3B1 was associated with shorter overall survival, while ALDH5A1 expression was associated with longer overall survival of pancreatic cancer patients. Clinicopathological analysis revealed a significant association with KRAS mutational status and ALDH3A1 expression. Immune correlation analysis indicated that high expression of ALDH3A1 and ALDH3B1 was associated with lower expression of CD8+ T cell-associated gene expression. ESTIMATE analyses further revealed that high expression of ALDH3A1 and ALDH3B1 was associated with lower levels of immune cell infiltration. PAAD tumors with low ALDH3A1 expression were more sensitive to paclitaxel. Immunohistochemical analysis demonstrated high expression of ALDH3A1 in pancreatic cancer cells of human tumor tissues compared to normal pancreatic tissues. Conclusions: This study unveils specific ALDH family members relevant for prognosis and chemotherapy response in pancreatic cancer patients. These findings contribute valuable insights into prognostic biomarkers and their potential clinical utility in the treatment of pancreatic adenocarcinoma
MLUH-E-380_2, Milvus milvus milvus (Linnaeus, 1758), eggshell
No full textInventory No.: MLUH-E-380_2, Object: eggshell, Species: Milvus milvus milvus (Linnaeus, 1758), Preservation: complete preservation, Locality_loc.: Schielheide Obersdorf, Locality today: Germany, Country: GermanyDate: 21/05/1916, Collection_coll.: M. Schönwetter, published in Handbuch der Oologie, Schönwetter, Max: Vol. I, p. 156, Identification by: M. Schönwetter, Aquisition: 1928, Aquired from: Dr. Henrici, ex Collection: ex. Coll. Dr. Henric
Global meta-analysis shows that threatened flowering plants have higher pollination deficitshHanyang Lin, Tiffany M. Knight [und viele weitere]
No full textMost flowering plant species rely on animal pollinators to reproduce, but insufficient pollen receipt, or pollen limitation, commonly occurs and is mediated by plant traits. Pollen limitation could either exacerbate extinction threat or arise as a consequence of population and range declines in threatened plants, leading to the expectation that pollen limitation should be higher in threatened compared to non-threatened plants. To test this, we perform a meta-analysis on a global dataset of pollen limitation from 2633 pollen supplementation experiments, integrating plant threat status and thirteen reproduction and life history traits. Threatened plant species have 26% higher levels of pollen limitation than non-threatened species. This pattern is moderated by plant traits and geographic location: we find higher levels of pollen limitation for threatened compared to non-threatened species for pollinator-dependent plants and for plants found in Asia and temperate zones. Using path analysis, we find that plant traits, study region, and threat status are causally linked to pollen limitation. We suggest that plant traits such as autofertility, which strongly predict pollen limitation, should be considered in global databases on plant threat. Further, preventing pollen limitation through habitat and pollinator management is a promising path to preventing plant extinction
MLUH-E-423_1, Pernis apivorus (Linnaeus, 1758), eggshell
No full textInventory No.: MLUH-E-423_1, Object: eggshell, Species: Pernis apivorus (Linnaeus, 1758), Preservation: complete preservation, Locality_loc.: Eichsfeld, Locality today: Eichsfeld, Country: GermanyDate: ?, Collection_coll.: M. Schönwetter, published in Handbuch der Oologie, Schönwetter, Max: Vol. I, p. 155, Identification by: M. Schönwetter, Aquisition: 1941, Aquired from: Dr. Henrici, ex Collection: ex. Coll. Dr. Henric
MLUH-E-57_4, Falco columbarius Linnaeus, 1758, eggshell
No full textInventory No.: MLUH-E-57_4, Object: eggshell, Species: Falco columbarius Linnaeus, 1758, Preservation: complete preservation, Locality_loc.: Pendle Hill; Lancashire, Locality today: Pendle Hill, Country: Great BritainCollector_leg.: R. H. Wrigly, Date: 27/05/1882, Collection_coll.: M. Schönwetter, published in Handbuch der Oologie, Schönwetter, Max: Vol. I, p. 191, Identification by: M. Schönwetter, Aquisition: 1934, Aquired from: C.H. Gowland, ex Collection: ex. Coll. C. H. Gowland; ex. Coll. R. H. Wrigl
Identification of a proteolysis-targeting-chimera that addresses activated checkpoint kinase-1 reveals its non-catalytic functions in tumor cells
No full textCheckpoint kinase-1 (CHK1) controls DNA replication and repair. Tumor cells depend on CHK1, whose high levels are associated with worse patient prognosis. We define a bona fide proteolysis-targeting-chimera (PROTAC) for CHK1. PROTAC MA203 contains the type I kinase inhibitor rabusertib, which preferentially inhibits activated CHK1, and the cereblon (CRBN) ligand pomalidomide. MA203 accelerates CRBN-dependent proteasomal degradation of CHK1 in solid tumor-derived cells and acute leukemia cells. Chemotherapy-induced DNA replication stress and a consequent activation of CHK1 accelerate this event-driven process which promotes DNA damage and tumor cell apoptosis. Biochemical and cellular target engagement studies confirm the potency and selectivity of MA203. MA203 does not damage healthy differentiated and primitive hematopoietic cells, stromal cells, and retinal epithelial cells. MA203 is superior to its corresponding kinase inhibitor concerning DNA damage, dysregulation of BCL2 proteins, and apoptosis induction. These processes occur independently of the tumor-suppressive transcription factor p53. Elimination of CHK1 protein as structural element, but not its inhibition per se, triggers a proteasomal degradation of key DNA replication and repair proteins. Genetic CHK1 elimination confirms that such newly recognized functions of CHK1 rely on functions beyond its well-known catalytic activity. Thus, kinase-independent functions of CHK1 can be exploited with innovative pharmacological agents
MLUH-E-394_1, Milvus migrans (Boddaert, 1783), eggshell
No full textInventory No.: MLUH-E-394_1, Object: eggshell, Species: Milvus migrans (Boddaert, 1783), Preservation: slight damage, Locality_loc.: Panam S. Tibet, Locality today: Bainang County, Country: ChinaCollector_leg.: Dr. E. Schäfer, Date: 21/05/1939, Collection_coll.: M. Schönwetter, published in Handbuch der Oologie, Schönwetter, Max: Vol. I, p. 157, Identification by: M. Schönwetter, Aquisition: 1940, Aquired from: Dr. E. Schäfer, ex Collection: ex. Coll. Dr. E. Schäfe